A shift in transitional forests of the North American boreal will persist through 2100.

High northern latitude changes with Arctic amplification across a latitudinal forest gradient suggest a shift towards an increased presence of trees and shrubs. The persistence of change may depend on the future scenarios of climate and on the current state, and site history, of forest structure. Here, we explore the persistence of a gradient-based shift in the boreal by connecting current forest patterns to recent tree cover trends and future modeled estimates of canopy height through 2100. Results show variation in the predicted potential height changes across the structural gradient from the boreal forest through the taiga-tundra ecotone. Positive potential changes in height are concentrated in transitional forests, where recent positive changes in cover prevail, while potential change in boreal forest is highly variable. Results are consistent across climate scenarios, revealing a persistent biome shift through 2100 in North America concentrated in transitional landscapes regardless of climate scenario.

Audio tagging of avian dawn chorus recordings in California, Oregon and Washington.

Background: Declines in biodiversity and ecosystem health due to climate change are raising urgent concerns. In response, large-scale multispecies monitoring programmes are being implemented that increasingly adopt sensor-based approaches such as acoustic recording. These approaches rely heavily on ecological data science. However, developing reliable algorithms for processing sensor-based data relies heavily on labelled datasets of sufficient quality and quantity. We present a dataset of 1,575 dawn chorus soundscape recordings, 141 being fully annotated (n = 32,994 annotations) with avian, mammalian and amphibian vocalisations. The remaining recordings were included to facilitate novel research applications. These recordings are paired with 48 site-level climatic, forest structure and topographic covariates. This dataset provides a valuable resource to researchers developing acoustic classification algorithms or studying biodiversity and wildlife behaviour and its relationship to environmental gradients. The dawn chorus recordings were collected as part of a long-term Northern Spotted Owl monitoring program; this demonstrates the complementary value of harnessing existing monitoring efforts to strengthen biodiversity sampling. New information: This dataset of dawn chorus soundscape recordings is one of the few open-access acoustic datasets annotated with non-biotic and both interspecific (across species) and intraspecific (within species) bird, mammal and amphibian sonotypes and the first that is paired with climatic, forest structure and topographical covariates extracted at recorder locations. This makes it a valuable resource for researchers studying the dawn chorus and its relationship to the environment.

Ectomycorrhizal fungi alter soil food webs and the functional potential of bacterial communities.

Most of Earth's trees rely on critical soil nutrients that ectomycorrhizal fungi (EcMF) liberate and provide, and all of Earth's land plants associate with bacteria that help them survive in nature. Yet, our understanding of how the presence of EcMF modifies soil bacterial communities, soil food webs, and root chemistry requires direct experimental evidence to comprehend the effects that EcMF may generate in the belowground plant microbiome. To this end, we grew Pinus muricata plants in soils that were either inoculated with EcMF and native forest bacterial communities or only native bacterial communities. We then profiled the soil bacterial communities, applied metabolomics and lipidomics, and linked omics data sets to understand how the presence of EcMF modifies belowground biogeochemistry, bacterial community structure, and their functional potential. We found that the presence of EcMF (i) enriches soil bacteria linked to enhanced plant growth in nature, (ii) alters the quantity and composition of lipid and non-lipid soil metabolites, and (iii) modifies plant root chemistry toward pathogen suppression, enzymatic conservation, and reactive oxygen species scavenging. Using this multi-omic approach, we therefore show that this widespread fungal symbiosis may be a common factor for structuring soil food webs.IMPORTANCEUnderstanding how soil microbes interact with one another and their host plant will help us combat the negative effects that climate change has on terrestrial ecosystems. Unfortunately, we lack a clear understanding of how the presence of ectomycorrhizal fungi (EcMF)-one of the most dominant soil microbial groups on Earth-shapes belowground organic resources and the composition of bacterial communities. To address this knowledge gap, we profiled lipid and non-lipid metabolites in soils and plant roots, characterized soil bacterial communities, and compared soils amended either with or without EcMF. Our results show that the presence of EcMF changes soil organic resource availability, impacts the proliferation of different bacterial communities (in terms of both type and potential function), and primes plant root chemistry for pathogen suppression and energy conservation. Our findings therefore provide much-needed insight into how two of the most dominant soil microbial groups interact with one another and with their host plant.

Occurrence patterns of sympatric forest wallabies: assessing the influence of structural habitat attributes on the coexistence of Thylogale thetis and T. stigmatica.

Background: We studied the occurrence of two sympatric wallabies, the red-necked pademelon (Thylogale thetis) and the red-legged pademelon (T. stigmatica) in northeastern New South Wales, Australia in relation to structural habitat attributes. At our study site, both species inhabit closed forest environments and have overlapping distributions, but T. thetis leaves the forest at night to graze adjacent grassy forest edges whereas T. stigmatica remains within the forest and browses forest vegetation. The objectives of the study were to investigate how structural attributes of two forest types, wet sclerophyll forest and rainforest, relate to the fine-scale occurrence of these two wallaby species within the forested environment. Methods: We gathered occurrence data from 48 camera trap stations divided equally between rainforest and wet sclerophyll forest. At each camera point, we also measured a range of structural habitat attributes to determine habitat affiliations for the two Thylogale species. Principal component analyses were used to describe major trends in habitat, and generalised linear models were used to describe the efficacy of the variables in predicting habitat occurrence of each species. Results: The number of occurrences of Thylogale thetis was significantly greater than occurrences of T. stigmatica, which was driven by significantly greater occurrences of T. thetis in wet sclerophyll forest. There was both spatial and temporal partitioning between the two species; there was a significant difference in the occurrences of the two species at individual cameras and T. stigmatica had a different activity schedule than T. thetis in wet sclerophyll forest, where the latter reached its greatest rate of occurrence. At a finer (camera station) scale, occurrences of T. thetis increased with proximity to roads and grassy edges and at sites that were less rocky and less steep. T. stigmatica occurrence increased in the presence of rainforest elements like vines, palms and ferns, more ground-level cover and tree-fall gaps and at sites with fewer emergent eucalypts. Conclusion: Our findings have implications for managing these pademelons and their habitats. T. thetis is a common species that was encountered more often than T. stigmatica, and it responded positively to human disturbance like roadsides and grassy edges, presumably because these areas provided good grazing opportunities. By comparison, T. stigmatica is a threatened species, and it responded to natural disturbance like tree-fall gaps where lateral cover was greater, and where rainforest food plants may be more abundant. Our results suggest, therefore, that conservation of the threatened T. stigmatica requires the preservation of intact rainforest.

Age-Related Conservation in Plant-Soil Feedback Accompanied by Ectomycorrhizal Domination in Temperate Forests in Northeast China.

This study investigates the effects of forest aging on ectomycorrhizal (EcM) fungal community and foraging behavior and their interactions with plant-soil attributes. We explored EcM fungal communities and hyphal exploration types via rDNA sequencing and investigated their associations with plant-soil traits by comparing younger (~120 years) and older (~250 years) temperate forest stands in Northeast China. The results revealed increases in the EcM fungal richness and abundance with forest aging, paralleled by plant-soil feedback shifting from explorative to conservative nutrient use strategies. In the younger stands, Tomentella species were prevalent and showed positive correlations with nutrient availability in both the soil and leaves, alongside rapid increases in woody productivity. However, the older stands were marked by the dominance of the genera Inocybe, Hymenogaster, and Otidea which were significantly and positively correlated with soil nutrient contents and plant structural attributes such as the community-weighted mean height and standing biomass. Notably, the ratios of longer-to-shorter distance EcM fungal exploration types tended to decrease along with forest aging. Our findings underscore the integral role of EcM fungi in the aging processes of temperate forests, highlighting the EcM symbiont-mediated mechanisms adapting to nutrient scarcity and promoting sustainability in plant-soil consortia.

Physiological meaning of bimodal tree growth-climate response patterns.

Correlation coefficients are widely used to identify and quantify climate signals in proxy archives. Significant relationships between tree-ring chronologies and meteorological measurements are typically applied by dendroclimatologists to distinguish between more or less relevant climate variation for ring formation. While insignificant growth-climate correlations are usually found with cold season months, we argue that weak relationships with high summer temperatures not necessarily disprove their importance for xylogenesis. Here, we use maximum latewood density records from ten treeline sites between northern Scandinavia and southern Spain to demonstrate how monthly growth-climate correlations change from narrow unimodal to wide bimodal seasons when vegetation periods become longer and warmer. Statistically meaningful relationships occur when minimum temperatures exceed 'biological zero' at around 5° C. We conclude that the absence of evidence for statistical significance between tree growth and the warmest summer temperatures at Mediterranean sites is no evidence of absence for the physiological importance of high summer temperatures for ring formation. Since correlation should never be confused with causation, statistical values require mechanistic understanding, and different interpretations are needed for insignificant correlations within and outside the growing season.

Brushing aside doubts: an evaluation of the beat-sheet brushing technique for detecting the Nearctic tree trunk sheetweaver (Araneae: Linyphiidae).

It is important to have reliable information on the presence/absence, population structure, and density of animals across their natural range. Detecting small organisms, however, such as the Nearctic tree trunk sheetweaver spider Drapetisca alteranda Chamberlin 1909 (Araneae: Linyphiidae), presents challenges due to its diminutive size and cryptic nature. We used a capture/recapture study to determine the detection and recapture probabilities of this spider using a standard beat sheet technique adopted for surveying tree trunks. Spiders were released on 3 different tree species that provided a range of microhabitats, including variable bark surface area and furrow depth/width. Microhabitat features played a small role in the timing of spider recapture (i.e., slower rate of recapture as furrowing increased). However, our results demonstrated 100% detection across replicate experiments and individual recapture probabilities exceeding 90% in most situations, with no significant differences in recapture observed among tree species and with respect to tree circumference. Furthermore, we show that most spiders could be recaptured within 2 sampling revolutions around the tree trunk, and there was no difference in the probability of collecting male and female spiders (although they differ markedly in size). Finally, we found no difference among brushers, supporting the idea that this method is replicable across collectors and studies. Collectively, we establish confidence in the ecological knowledge obtained with this technique and encourage its application with similar species and systems.

Unpunctual in diversity: The effect of stand species richness on spring phenology of deciduous tree stands varies among species and years.

Climatic drivers alone do not adequately explain the regional variation in budburst timing in deciduous forests across Europe. Stand-level factors, such as tree species richness, might affect budburst timing by creating different microclimates under the same site macroclimate. We assessed different phases of the spring phenology (start, midpoint, end, and overall duration of the budburst period) of four important European tree species (Betula pendula, Fagus sylvatica, Quercus robur and Tilia cordata) in monocultures and four-species mixture stands of a common garden tree biodiversity experiment in Belgium (FORBIO) in 2021 and 2022. Microclimatic differences between the stands in terms of bud chilling, temperature forcing, and soil temperature were considerable, with four-species mixtures being generally colder than monocultures in spring, but not in winter. In the colder spring of 2021, at the stand level, the end of the budburst period was advanced, and its overall duration shortened, in the four-species mixtures. At species level, this response was significant for F. sylvatica. In the warmer spring of 2022, advances in spring phenology in four-species stands were observed again in F. sylvatica and, less markedly, in B. pendula but without a general response at the stand level. Q. robur showed specific patterns with delayed budburst start in 2021 in the four-species mixtures and very short budburst duration for all stands in 2022. Phenological differences between monocultures and four-species mixtures were linked to microclimatic differences in light availability rather than in temperature as even comparatively colder microclimates showed an advanced phenology. Compared to weather conditions, tree species richness had a lower impact on budburst timing, but this impact can be of importance for key species like F. sylvatica and colder springs. These results indicate that forest biodiversity can affect budburst phenology, with wider implications, especially for forest- and land surface models.

Mycorrhization in trees: ecology, physiology, emerging technologies and beyond.

Mycorrhization has been an integral part of plants since colonization by the early land plants. Over decades, substantial research has highlighted its potential role in improving nutritional efficiency and growth, development and survival of crop plants. However, the focus of this review is trees. Evidence have been provided to explain ecological and physiological significance of mycorrhization in trees. Advances in recent technologies (e.g., metagenomics, artificial intelligence, machine learning, agricultural drones) may open new windows to apply this knowledge in promoting tree growth in forest ecosystems. Dual mycorrhization relationships in trees and even triple relationships among trees, mycorrhizal fungi and bacteria offer an interesting physiological system to understand how plants interact with other organisms for better survival. Besides, studies indicate additional roles of mycorrhization in learning, memorizing and communication between host trees through a common mycorrhizal network (CMN). Recent observations in trees suggest that mycorrhization may even promote tolerance to multiple abiotic (e.g., drought, salt, heavy metal stress) and biotic (e.g. fungi) stresses. Due to the extent of physiological reliance, local adaptation of trees is heavily impacted by the mycorrhizal community. This knowledge opens the possibility of a non-GMO avenue to promote tree growth and development. Indeed, mycorrhization could impact growth of trees in nurserys and subsequent survival of the inoculated trees in field conditions. Future studies might integrate hyperspectral imaging and drone technologies to identify tree communities that are deficient in nitrogen and spray mycorrhizal spore formulations on them.

Trophic niches of Collembola communities change with elevation, but also with body size and life form.

Climate change will likely increase habitat loss of endemic tree species and drives forest conversion in mountainous forests. Elevation gradients provide the opportunity to predict possible consequences of such changes. While species compositions of various taxa have been investigated along elevation gradients, data on trophic changes in soil-dwelling organisms are scarce. Here, we investigated trophic changes of the Collembola communities along the northern slope of Changbai Mountain, China. We sampled Collembola in primary forests at seven elevations (800-1700 m asl). We measured individual body lengths and bulk stable isotopes on species level. We further categorized Collembola species into life forms. The community-weighted means of Δ15N and Δ13C values as well as minimum Δ15N values and isotopic uniqueness of Collembola communities increased with increasing elevation, while the range of Δ15N values decreased. Maximum and minimum of Δ13C values differed between elevations but showed no linear trend. Further, Δ15N values of Collembola species occurring across all elevations increased with elevation. Changes in Δ15N values with elevation were most pronounced in hemiedaphic species, while Δ13C values increased strongest with elevation in euedaphic species. Δ15N values increased with decreasing body size in hemiedaphic and euedaphic species. Overall, the results suggest that Collembola species functioning as primary decomposers at lower elevations shift towards functioning as secondary decomposers or even predators or scavengers at higher elevation forests. The results further indicate that access to alternative food resources depends on Collembola life form as well as body size and varies between ecosystems.

On functional groups and forest dynamics.

Functional trait variation measured on continuous scales has helped ecologists to unravel important ecological processes. However, forest ecologists have recently moved back toward using functional groups. There are pragmatic and biological rationales for focusing on functional groups. Both of these approaches have inherent limitations including binning clearly continuous distributions, poor trait-group matching, and narrow conceptual frameworks for why groups exist and how they evolved. We believe the pragmatic use of functional groups due to data deficiencies will eventually erode. Conversely, we argue that existing conceptual frameworks for why a limited number of tree functional groups may exist is a useful, but flawed, starting point for modeling forests that can be improved through the consideration of unmeasured axes of functional variation.

Biochar effects on NTFP-enriched secondary forest growth and soil properties in Amazonian Ecuador.

Deforestation in the Amazon has resulted in large areas of depleted soils on abandoned pastures and agricultural sites that present a restoration challenge central to protecting biodiversity and ecosystem function in the region. Biochar - charcoal made from waste materials - can improve soil physical, chemical, and biological properties, but the few tropical field trials to date do not give consistent results regarding tree growth. This study presents three years of soil performance and tree growth of a secondary forest shading nontimber forest product (NTFP) plantations of Ocotea quixos (Lauraceae), Myroxylon balsamum (Fabaceae), and their mixture. Open kiln and traditional mound biochars were added at 10 t ha-1 at two sites with contrasting soil types. Biochar additions resulted in pronounced effects on soil properties that varied over time and with depth in the soil profile. Biochar additions generally increased soil organic matter, electrical conductivity, and plant nutrients (in particular K, Ca, and N), but there were interactive effects of NTFP treatments, and stronger responses on the poorer soil type. Biochar amendments resulted in increased tree growth, with a 29 ± 12% increase in aboveground biomass (AGB) on plots amended with kiln biochar and a 23 ± 9% increase in plots with mound biochar compared to controls. Tree species also varied in growth responses to biochar additions, with the largest increases observed in Jaccaranda copaia and Piptocoma discolor. Significant interactions between biochar and NTFP treatments were also seen for tree growth responses, such as Cecropia spp., which only showed increased biomass on mound biochar plots planted with Ocotea quixos. Overall, our results demonstrate a stronger effect of biochar in less favorable soil conditions, and an overriding effect of the legume NTFP in richer soils, and suggest that additions of biochar and legumes are important options to increase productivity and ecological resilience in tropical forest restoration.

The geophysical toolbox applied to forest ecosystems - A review.

Studying the forest subsurface is a challenge because of its heterogeneous nature and difficult access. Traditional approaches used by ecologists to characterize the subsurface have a low spatial representativity. This review article illustrates how geophysical techniques can and have been used to get new insights into forest ecology. Near-surface geophysics offers a wide range of methods to characterize the spatial and temporal variability of subsurface properties in a non-destructive and integrative way, each with its own advantages and disadvantages. These techniques can be used alone or combined to take advantage of their complementarity. Our review led us to define three topics how near-surface geophysics can support forest ecology studies: 1) detection of root systems, 2) monitoring of water quantity and dynamics, and 3) characterisation of spatial heterogeneity in subsurface properties at the stand level. The number of forest ecology studies using near-surface geophysics is increasing and this multidisciplinary approach opens new opportunities and perspectives for improving quantitative assessment of biophysical properties and exploring forest response to the environment and adaptation to climate change.

Giants of the Amazon: How does environmental variation drive the diversity patterns of large trees?

For more than three decades, major efforts in sampling and analyzing tree diversity in South America have focused almost exclusively on trees with stems of at least 10 and 2.5 cm diameter, showing highest species diversity in the wetter western and northern Amazon forests. By contrast, little attention has been paid to patterns and drivers of diversity in the largest canopy and emergent trees, which is surprising given these have dominant ecological functions. Here, we use a machine learning approach to quantify the importance of environmental factors and apply it to generate spatial predictions of the species diversity of all trees (dbh ≥ 10 cm) and for very large trees (dbh ≥ 70 cm) using data from 243 forest plots (108,450 trees and 2832 species) distributed across different forest types and biogeographic regions of the Brazilian Amazon. The diversity of large trees and of all trees was significantly associated with three environmental factors, but in contrasting ways across regions and forest types. Environmental variables associated with disturbances, for example, the lightning flash rate and wind speed, as well as the fraction of photosynthetically active radiation, tend to govern the diversity of large trees. Upland rainforests in the Guiana Shield and Roraima regions had a high diversity of large trees. By contrast, variables associated with resources tend to govern tree diversity in general. Places such as the province of Imeri and the northern portion of the province of Madeira stand out for their high diversity of species in general. Climatic and topographic stability and functional adaptation mechanisms promote ideal conditions for species diversity. Finally, we mapped general patterns of tree species diversity in the Brazilian Amazon, which differ substantially depending on size class.

Positive interactions between mycorrhizal fungi and bacteria are widespread and benefit plant growth.

Bacteria, ectomycorrhizal (EcM) fungi, and land plants have been coevolving for nearly 200 million years, and their interactions presumably contribute to the function of terrestrial ecosystems. The direction, stability, and strength of bacteria-EcM fungi interactions across landscapes and across a single plant host, however, remains unclear. Moreover, the genetic mechanisms that govern them have not been addressed. To these ends, we collected soil samples from Bishop pine forests across a climate-latitude gradient spanning coastal California, fractionated the soil samples based on their proximity to EcM-colonized roots, characterized the microbial communities using amplicon sequencing, and generated linear regression models showing the impact that select bacterial taxa have on EcM fungal abundance. In addition, we paired greenhouse experiments with transcriptomic analyses to determine the directionality of these relationships and identify which genes EcM-synergist bacteria express during tripartite symbioses. Our data reveal that ectomycorrhizas (i.e., EcM-colonized roots) enrich conserved bacterial taxa across climatically heterogeneous regions. We also show that phylogenetically diverse EcM synergists are positively associated with plant and fungal growth and have unique gene expression profiles compared with EcM-antagonist bacteria. In sum, we identify common mechanisms that facilitate widespread and diverse multipartite symbioses, which inform our understanding of how plants develop in complex environments.

Germination temperature sensitivity differs between co-occurring tree species and climate origins resulting in contrasting vulnerability to global warming.

Climate change is shifting temperatures from historical patterns, globally impacting forest composition and resilience. Seed germination is temperature-sensitive, making the persistence of populations and colonization of available habitats vulnerable to warming. This study assessed germination response to temperature in foundation trees in south-western Australia's Mediterranean-type climate forests (Eucalyptus marginata (jarrah) and Corymbia calophylla (marri)) to estimate the thermal niche and vulnerability among populations. Seeds from the species' entire distribution were collected from 12 co-occurring populations. Germination thermal niche was investigated using a thermal gradient plate (5-40°C). Five constant temperatures between 9 and 33°C were used to test how the germination niche (1) differs between species, (2) varies among populations, and (3) relates to the climate of origin. Germination response differed among species; jarrah had a lower optimal temperature and thermal limit than marri (T o 15.3°C, 21.2°C; ED50 23.4°C, 31°C, respectively). The thermal limit for germination differed among populations within both species, yet only marri showed evidence for adaptation to thermal origins. While marri has the capacity for germination at higher thermal temperatures, jarrah is more vulnerable to global warming exceeding safety margins. This discrepancy is predicted to alter species distributions and forest composition in the future.

A new non-destructive method for the estimation of annual height increment in standing trees.

Measurement of tree growth is needed in many scientific and production sectors, especially in forestry for wood and biomass production. The assessment of annual height increment in standing living trees, under ambient field conditions, is challenging or even impossible. This study develops a new simple non-destructive method for the estimation of annual height increment in standing trees, based on sampling two increment cores for each targeted tree.,. Τhe method combines the tree annual ring-analysis and trigonometry. The extracted data by the method application can be widely used in many forest disciplines, such as forest ecology, silviculture, and forest management.•Annual height increment in standing trees is a valuable parameter needed in biology, ecology, and forestry.•Measurement of annual height increment in standing trees is a very difficult tusk.•This study develops a new non-destructive and simple method for the estimation of annual height increment in standing trees.

Shedding light on the effects of climate and anthropogenic pressures on the disappearance of Fagus sylvatica in the Italian lowlands: evidence from archaeo-anthracology and spatial analyses.

Fagus sylvatica is one of the most representative trees of the European deciduous broadleaved forests, yet the impact of changing climatic conditions and anthropogenic pressures (anthromes) on its presence and distribution in the coastal and lowland areas of the Mediterranean Basin has long been overlooked. Here, we first analysed the local forest composition in two different time intervals (350-300 Before Current Era, BCE and 150-100 BCE) using charred wood remains from the Etruscan site of Cetamura (Tuscany, central Italy). Additionally, we reviewed all the relevant publications and the wood/charcoal data obtained from anthracological analysis in F. sylvatica, focusing on samples that date back to 4000 years before present, to better understand the drivers of beech presence and distribution during the Late Holocene (LH) in the Italian Peninsula. Then, we combined charcoal and spatial analyses to test the distribution of beech woodland at low elevation during LH in Italy and to evaluate the effect of climate change and/or anthrome on the disappearance of F. sylvatica from the lowlands. We collected 1383 charcoal fragments in Cetamura belonging to 21 woody taxa, with F. sylvatica being the most abundant species (28 %), followed by other broadleaved trees. We identified 25 sites in the Italian Peninsula with beech charcoals in the last 4000 years. Our spatial analyses showed a marked decrease in habitat suitability of F. sylvatica from LH to the present (ca. 48 %), particularly in the lowlands (0-300 m above sea level, a.s.l.) and in areas included between 300-600 m a.s.l. with a subsequent shift upwards of the beech woodland of ca. 200 m from the past to the present. In the lowland areas, where F. sylvatica has disappeared, anthrome alone and climate + anthorme had a main effect on beech distribution whitin 0-50 m a.s.l., while the climate from 50 to 300 m a.s.l. Furthermore, climate affect also the beech distrinution in the areas >300 m a.s.l., while climate + anthrome and antrhome alone were mainly focused on the lowland areas. Our results highlight the advantage of combining different approaches, such as charcoal analysis and spatial analyses, to explore biogeographic questions about the past and current distribution of F. sylvatica, with important implications for today's forest management and conservation policies.

Sunflower sea star predation on urchins can facilitate kelp forest recovery.

The recent collapse of predatory sunflower sea stars (Pycnopodia helianthoides) owing to sea star wasting disease (SSWD) is hypothesized to have contributed to proliferation of sea urchin barrens and losses of kelp forests on the North American west coast. We used experiments and a model to test whether restored Pycnopodia populations may help recover kelp forests through their consumption of nutritionally poor purple sea urchins (Strongylocentrotus purpuratus) typical of barrens. Pycnopodia consumed 0.68 S. purpuratus d-1, and our model and sensitivity analysis shows that the magnitude of recent Pycnopodia declines is consistent with urchin proliferation after modest sea urchin recruitment, and even small Pycnopodia recoveries could generally lead to lower densities of sea urchins that are consistent with kelp-urchin coexistence. Pycnopodia seem unable to chemically distinguish starved from fed urchins and indeed have higher predation rates on starved urchins owing to shorter handling times. These results highlight the importance of Pycnopodia in regulating purple sea urchin populations and maintaining healthy kelp forests through top-down control. The recovery of this important predator to densities commonly found prior to SSWD, whether through natural means or human-assisted reintroductions, may therefore be a key step in kelp forest restoration at ecologically significant scales.