ABSTRACT
ABSTRACT BACKGROUND: Extracts obtained from plants and fruits provide a relatively safe and practical alternative for the conventional medicine of gastrointestinal diseases. The specie Eugenia mattosii, popularly known in Brazil as "cerejinha", belongs to Myrtaceae family. Species of this family present pharmacological properties, and can be used in the treatment of gastrointestinal disorders. OBJECTIVE: The aim of this study was to determine the phytochemical profile and evaluate the gastroprotective activity of Eugenia mattosii fruits. METHODS: Phytochemical analysis was carried out by thin layer chromatography and gastroprotective assays were performed using two experimental models: acute ulcer model induced by ethanol/HCl and acute ulcer model induced by non-steroidal anti-inflammatory drug (indomethacin). Total lesion area (mm2) and relative lesion area (%) were determined. RESULTS: The results of the phytochemical analysis indicated that the bark and pulp and seeds of E. mattosii present phenolic compounds, terpenes and/or steroids. In gastric ulcer model induced by ethanol was evidenced significant reduction of damaged areas for doses of 50 and 250 mg/ kg of seeds methanol extract, while in the indomethacin-induced ulcer model, all parts of the fruit presented defense capability of the gastric mucosa by reducing lesions at doses of 50, 125 and 250 mg/kg. CONCLUSION: The results demonstrate that the specie E. mattosii has bioactive compounds that provide gastroprotective activity, presenting possible therapeutic potential.
RESUMO CONTEXTO: Extratos obtidos de plantas e frutos fornecem uma alternativa relativamente segura e prática para os remédios convencionais de doenças gastrointestinais. A espécie Eugenia mattosii, popularmente conhecida no Brasil como "cerejinha", pertence à família Myrtaceae. Espécies desta família apresentam propriedades farmacológicas e podem ser utilizadas no tratamento de distúrbios gastrointestinais. OBJETIVO: O objetivo deste estudo foi determinar o perfil fitoquímico e avaliar a atividade gastroprotetora dos frutos de Eugenia mattosii. MÉTODOS: A análise fitoquímica foi realizada por cromatografia em camada delgada e dois modelos experimentais foram utilizados para avaliação da atividade gastroprotetora em camundongos: modelo de úlcera gástrica induzida por anti-inflamatório não-esteroidal (indometacina) e modelo de úlcera gástrica induzida por etanol/HCl. RESULTADOS: Os resultados da análise fitoquímica indicaram que a casca e polpa e as sementes de E. mattosii apresentam compostos fenólicos, terpenos e/ou esteroides. No modelo de úlcera gástrica induzido pelo etanol, foi evidenciada redução significativa de áreas danificadas para doses de 50 e 250 mg/kg do extrato das sementes, enquanto no modelo de úlcera induzida por indometacina, todas as partes do fruto apresentaram capacidade de defesa da mucosa gástrica ao reduzir as lesões nas doses de 50, 125 e 250 mg/kg. CONCLUSÃO: Os resultados demonstram que a espécie E. mattosii possui compostos bioativos com atividade gastroprotetora, apresentando possível potencial terapêutico.
Subject(s)
Animals , Female , Mice , Stomach Ulcer/prevention & control , Plant Extracts/pharmacology , Protective Agents/pharmacology , Eugenia/chemistry , Fruit/chemistry , Gastric Mucosa/drug effects , Anti-Ulcer Agents/pharmacology , Seeds/chemistry , Stomach Ulcer/chemically induced , Brazil , Plant Extracts/administration & dosage , Indomethacin , Disease Models, Animal , Ethanol , Phytochemicals/pharmacology , Phytotherapy , Anti-Ulcer Agents/administration & dosage , Antioxidants/analysis , Antioxidants/pharmacologyABSTRACT
[ Objective] To compare the protective mechanism of Zuojin Pill (Rhizoma Coptidis: Fructus Evodiae = 6:1) and Fan Zuojin Pill (Rhizoma Coptidis: Fructus Evodiae = 1: 6) on gastric secretion and gastric mucosal barrier. [Methods] Pylorus - ligated rat model was applied. Rats were divided into normal saline group, Zuojin Pill groups (1.4 g/kg and 2.8 g/kg) and Fan Zuojin Pill groups (1.4g/kg and 2.8g/kg) and then the drugs were given by duodenal feeding. Gastric secretion and gastric mucus synthesis were examined. [Results] Zuojin Pill and Fan Zuojin Pill (1.4 g/kg and 2.8 g/kg) both inhibited gastric secretion and decreased total acid output and pepsin activity in a dose- effect manner. Zuojin Pill and Fan Zuojin Pill also increased the content of gastric adherent mucus (GAM) and tended to elevate the mucus level in gastric juice. [Conclusion] The preventive mechanism of Zuojin Pill and Fan Zuojin Pill on gastric mucosa may be related to the regulation of gastric secretion and gastric mucus synthesis.
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[Objective] To observe the effects of blood-activating and stasis-removing (BASR) herbs on the proliferation and apoptosis of gastric mucosa cells (GMC) in experimental chronic atrophic gastritis (CAG) rats. [Methods] CAG rat models were established with comprehensive method and then were given BASR herbs. Argyrophilic protein of nucleolar organizer regions ( AgNOR) count was carried out after silver nitrate staining. The expression of proliferating cell nuclear antigen (PCNA) was examined with immunohistochemical method and apoptosis was detected by in-situ cell-end labeling method. [Results] The number of AgNOR and the indexes of PCNA and apoptosis were increased in CAG model rats ( P
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AIM: To study the effects of extracts B1, B2, and B3 from faeces trogopterorum on the experimental gastric ulcer in rats. METHODS: Two different animal models of gastric ulcers were used in this experiment: Shay's model (n = 72) and the reserpine-induced ulcer model (n = 76). The total volume and the pH of the gastric juices were recorded. The lesion scores of gastric mucosa were also recorded. RESULTS: The lesion scores of gastric mucosa in the Shay's model of animals in the WLZ-B1 groups treated with either 40 g/kg or 80 g/kg were 8.6 ± 10.8 and 1.6 ± 1.9 respectively, which were lower than that of the 0.9% NaCl control group (47.0 ± 31.4, P < 0.05, P < 0.01). The lesion scores for the 80 g/kg group was lower compared to those of the Ran group (20.5 ± 16.4, P < 0.01). The pH of the gastric juices of the 80 g/kg group (3.425 ± 0.143) was higher than that of the 0.9% NaCl group (2.836 ± 0.632, P < 0.05). In the reserpine model, the lesion score of the 40 g/kg group of the WLZ-B1 (20.7 ± 16.5) was also lower than that of the 0.9% NaCl control group (76.3 ± 50.6, P < 0.05). CONCLUSION: B1 is the most effective of the three sections in inhibiting gastric secretion, protecting gastric mucosa and preventing experimental ulceration.
