ABSTRACT
Objective: A reversed phase HPLC method was described for determination of prim O glucosylcimifugin and 4′ O ? D glucosyl 5 O methylvisamminol in Ganmaoqingre Granules. Methods:The sample was separated on ODS column with mobile phase of methanol 40 mmol?L -1 sodium acetate pH 6.9 (35∶65) for prim O glucosylcimifugin and H 2O methanol THF(62∶38∶1) for 4′ O ? glucosyl 5 O methylvisamminol. The flow rate was 0.8 mL?min -1 , and the detection was set at 254 nm. Results: The calibration curves were linear in the range of 0.72 ?g?mL -1 ～6.5?g?mL -1 for prim O glucosylcimifugin and 0.92?g?mL -1 ～16.5?g?mL -1 for 4′ O ? D glucosyl 5 O methylvisamminol( r =0.9999). The average recovery was 100.3% and 94.7%. The content of prim O glucosylcimifugin and 4′ O ? D glucosyl 5 O methylvisamminol in Ganmaoqingre Granules was 0.133 mg?g -1 and 0.167 mg?g -1 , respectively. Conclusion: The method is fast and specific for both constitutents of Ganmaoqingre Granules.
