Potential of vermicomposting with mixtures of animal manure and vegetable leaves in the development of Eisenia foetida, microbial biomass, and enzymatic activity under semi-arid conditions.

Vermicomposting is the bio-oxidation and stabilization of organic matter involving relationships between the action of earthworms and microorganisms and the activation and dynamics of several enzyme activities. Semi-arid farmers to make (extra) money and organic production, produce their vermicompost using plant residues and animal manure, but there is no information about the final product generated. Thus, this study aimed to analyze the potential of vermicomposting with mixtures of animal manure and vegetable leaves in the development of Eisenia foetida, microbial biomass, and enzymatic activity in the semi-arid region, Brazil. The experimental design applied was randomized block in a 6 × 4 factorial scheme with four replicates, with six treatments (mixtures of cattle manure, goat manure, cashew leaves, and catanduva leaves) and evaluated at four-time intervals (30, 60, 90, and 120 days of vermicomposting). The treatments were placed in polyethylene pots in the same site, environmental conditions, and residues proportions as used by farmers. The characteristics analyzed were the number of earthworms (NE), total earthworm biomass (TEB) and earthworm multiplication index (MI), microbial biomass carbon (MBC), and activities of enzymes ß-glucosidase, dehydrogenase, alkaline and acid phosphatases. The cattle manure vermicomposted shows the highest average values observed for NE, MI, TEB, MBC, and enzymatic activity, regardless of the plant leaves mix. In general, the enzymes activities were found in the descending order of ß-glucosidase > alkaline phosphatase > dehydrogenase > acid phosphatase. The maturation dynamics of vermicompost were characterized by a decline in the microbial population and number and biomass of earthworms in the substrate and consequently a decrease in new enzyme synthesis and degradation of the remaining enzyme pool. Microbial biomass and enzymatic activity were indicators for changes in the quality of vermicompost.

Role of selenite on the nitrogen conservation and greenhouse gases mitigation during the goat manure composting process.

This study aimed to explore the roles of selenite (Se) on nitrogen conservation and greenhouse gases (GHGs) mitigation during the composting process. Six levels of Se(IV) dosages (i.e. 0, 2, 4, 6, 8 and 10 mg/kg) were examined for 80-day composting of goat manure and wheat straw mixtures, where the different blending proportions were marked as T1 (Control), T2, T3, T4, T5 and T6, respectively. The results showed that adding Se(IV) was beneficial for reducing NH3 by 3.50-42.41% by buffering pH and promoting nitrification. For N2O, it showed different responses to different Se(IV) dosages, and it was increased by 29.62-71.29% in T2-T4 but reduced by 30.45-69.54% in T5-T6. Methane (CH4), another main component of GHGs, was increased by 1.35-107.42% by adding 2-10 mg/kg Se(IV). To further evaluate the effect of Se(IV) on GHGs, global warming potential value was calculated, which was 103.32-499.80 and minimum value was in T5. Furthermore, the physicochemical indexes, especially temperature and OM, had vital effects on microbial community. Overall, the results obtained from this study demonstrated that the application of Se (IV) in composting was reasonable to generate Se-rich organic fertilizer, and the 8 mg/kg was suggested from perspectives of nitrogen conservation and GHGs reduction.

Accelerated atrazine degradation and altered metabolic pathways in goat manure assisted soil bioremediation.

The intensive and long-term use of atrazine in agriculture has resulted in serious environmental pollution and consequently endangered ecosystem and human health. Soil microorganisms play an important role in atrazine degradation. However, their degradation efficiencies are relatively low due to their slow growth and low abundance, and manure amendment as a practice to improve soil nutrients and microbial activities can solve these problems. This study investigated the roles of goat manure in atrazine degradation performance, metabolites and bacterial community structure. Our results showed that atrazine degradation efficiencies in un-amended soils were 26.9-35.7% and increased to 60.9-84.3% in goat manure amended treatments. Hydroxyatrazine pathway was not significantly altered, whereas deethylatrazine and deisopropylatrazine pathways were remarkably enhanced in treatments amended with manure by encouraging the N-dealkylation of atrazine side chains. In addition, goat manure significantly increased soil pH and contents of organic matters and humus, explaining the change of atrazine metabolic pathway. Nocardioides, Sphingomonas and Massilia were positively correlated with atrazine degradation efficiency and three metabolites, suggesting their preference in atrazine contaminated soils and potential roles in atrazine degradation. Our findings suggested that goat manure acts as both bacterial inoculum and nutrients to improve soil microenvironment, and its amendment is a potential practice in accelerating atrazine degradation at contaminated sites, offering an efficient, cheap, and eco-friendly strategy for herbicide polluted soil remediation.

Exhausted grape marc and organic residues composting with polyethylene cover: Process and quality evaluation as plant substrate.

In Argentina, wine production is one of the most important economic activities, producing a large quantities of organic wastes. Composting is a viable alternative to treat these residues, with the possibility to obtain high-quality products. The objective of the present study was to evaluate the effect of the addition of goat manure, leaves from garden raking and alfalfa to exhausted grape marc and the influence of plastic cover on the composting process and the quality of the finished product. Composting was carried out in turned piles in a total randomized design. Temperature, moisture, pH, electrical conductivity, micro and macro elements, biological parameters, stability and structure were determined with the goal to assess product quality. Mixture compost presented higher macro and micro elements content (especially P, Mn and Zn) than grape marc compost. Plastic cover showed no significant effect on physico-chemical parameters, while microorganisms were affected, with higher cellulolytic and nitrifying content in uncovered piles. In all composts, parameters indicated stability and no pathogens (Salmonella sp.) were detected. Pot assay with Lactuca sativa suggested that all compost revealed adequate substrate quality, with higher plant biomass values than commercial substrate and sand (Control), even with fertilization. Mixture compost showed significantly higher biomass and nutrient absorption values relative to the remaining evaluated substrates, suggesting that the use of goat manure and leaves added to exhausted grape marc, as raw material resulted in higher compost quality. All together indicated that the use of polyethylene cover negatively affect microorganisms content and a higher diversity of organic residues composted would result in higher microbiological activity and nutritional grade product.

Morfometria de cladódios de Opuntia stricta sob lâminas de irrigação e adubação orgânica no semiárido paraibano / Morphometry of Opuntia stricta cladodes submitted to different irrigation levels and organic fertilizer in the paraiba semi-arid region / Morfometría de cladodios de Opuntia stricta bajo cuchillas de riego y fertilización orgánica en el semiárido paraibano

A Palma Forrageira é uma planta xerófila de grande importância para a manutenção da pecuária nordestina, sobretudo, durante os períodos de estiagem. No entanto, diante da necessidade da recuperação dos palmais destruídos pela cochonilha do carmim, faz-se necessário conhecer os efeitos das práticas de manejo sobre as novas variedades introduzidas. Nesse sentido, este trabalho objetivou avaliar o efeito de diferentes lâminas de irrigação e fontes de adubação orgânica sobre a morfometria de cladódios da palma forrageira 'Orelha de Elefante Mexicana' (Opuntia stricta [Haw.] Haw.) cultivada no Semiárido. O experimento foi conduzido na Fazenda Riachão, Boa Vista, Paraíba. A área de plantio utilizada estava no segundo ciclo depois de dois anos de implantação. Utilizou-se um fatorial duplo 3 x 6 (tipos de adubação; lâminas de irrigação) em delineamento em blocos casualizados, com cinco repetições. Utilizaram-se dois tipos de esterco, bovino e caprino e a testemunha, associados com a ausência de irrigação e cinco lâminas diferentes (0; 1,5; 2,5; 3,5; 4,5 e 5,5 L de água por semana por planta). Ao fim de três meses, foram mensurados a área do cladódio, índice de área de cladódio e volume de cladódios total. Observou-se efeito significativo a 1% de probabilidade, pelo Teste F, da lâmina de irrigação sob todos os parâmetros analisados, sendo a lâmina de 5,5 L/semana a que propiciou os melhores resultados para a maioria das variáveis. Os tipos de esterco isoladamente não diferiram estatisticamente entre si, no entanto, o esterco caprino associado com a irrigação apresentou melhores resultados.(AU)

The forage palm is a xerophilous crop of great importance for the maintenance of livestock in the Northeast region of Brazil during periods of drought. However, due to the need to recover the palm trees destroyed by the prickly pear cochineal, it is important to know the effects of management practices on the new varieties that have been introduced in the area. In this sense, this study aimed at evaluating the effect of different irrigation levels and sources of organic fertilization on the morphometric attributes of forage palm cladodes of the Erect Prickly Pear (Opuntia stricta [Haw.] Haw.) grown in a semi-arid environment. The experiment was conducted at Fazenda Riachão, in the city of Boa Vista, state of Paraíba. The planting area used was at its second cycle after two years of implantation. Two types of manure ­ cattle and goat, as well as control, associated with absence of irrigation and five different levels of irrigation, ranging from 0; 1.5; 2.5; 3,.5; 4.5 and 5.5 L of water per week per plant were used. A 3 x 6 double factorial model (types of fertilization, irrigation levels) was used in a randomized block design, with five replications. At the end of a three-months' period, the cladode area, cladode area index, and total cladode volume were measured. A significant effect could be observed at 1% probability by F Test regarding the irrigation level for all analyzed parameters, with the 5.5 L/week irrigation mode providing the best results for most of the studied parameters. The manure types alone did not differ statistically from each other; however, the goat manure associated with irrigation presented better results than those for cattle manure.(AU)

La palma forrajera es una planta xerófila de gran importancia para el mantenimiento del ganado del noreste, especialmente durante los períodos de sequía. Sin embargo, en vista de la necesidad de recuperación de las palmas destruidas por la cochinilla carmínica, es necesario conocer los efectos de las prácticas de manejo en las nuevas variedades introducidas. En ese sentido, esta investigación tuvo como objetivo evaluar el efecto de diferentes cuchillas de riego y fuentes de fertilización orgánica en la morfometría de cladodios de la palma forrajera 'Oreja de Elefante Mexicano' (Opuntia stricta [Haw.] Haw.) cultivada en el Semiárido. El experimento se realizó en la Hacienda Riachão, Boa Vista, Paraíba. El área de plantación utilizada estaba en el segundo ciclo después de dos años de implantación. Se usó un factorial doble 3 x 6 (tipos de fertilización; cuchillas de riego) en un diseño de bloques al azar con cinco repeticiones. Se utilizaron dos tipos de estiércol, siendo de ganado y cabras y el control, asociados con la ausencia de riego y cinco diapositivas diferentes (0; 1.5; 2.5; 3.5; 4.5 y 5.5 L de agua por semana por planta). Después de tres meses, se midieron el área del cladodio, el índice del área del cladodio y el volumen total del cladodio. Se observó un efecto significativo con una probabilidad del 1%, mediante la Prueba F, de la cuchilla de riego bajo todos los parámetros analizados, con la cuchilla de 5.5 L/semana proporcionando los mejores resultados para la mayoría de las variables. Los tipos de estiércol por sí solos no difirieron estadísticamente, sin embargo, el estiércol de cabra asociado con el riego presentó mejores resultados.(AU)

Nutrient status and phytotoxicity analysis of goat manure discharged from farms in South Korea.

The present study evaluated the phytotoxicity effect of goat manure (GM) collected from six different regions in South Korea, namely, Chupungnyeong (T1), Hoengseong (T2), Goesan (T3), Sancheong (T4), Jangsu (T5) and Namwon (T6). Phytotoxicity was assessed by means of the analysis of germination index (GI), relative seed germination (RSG), relative root elongation (RRE) and vigor index (VI) using five commercial crop varieties, namely, sesame (Sesamum indicum L.), chrysanthemum (Chrysanthemum indicum L.), carrot (Daucus carota), radish (Raphanus sativus) and cabbage (Brassica rapa). Physico-chemical parameter values were recorded at appreciable levels in all GM extracts. The effect of seedling growth was significantly different (p ≤ .05) due to the variability of nutrient content and phytotoxic effect of the extracts on the different crop seeds. Of the extracts, Goesan (T3) and Sancheong (T4) recorded the best results in the range of GI (%) (54.1-128.8) and VI (930.7-1044) and GI (%) (70.1-167.3) and VI (609.2-3034), respectively, and also showed no inhibitory effect in any of the crop seeds. Overall results also revealed that radish crops showed excellent and non-phytotoxic results in all manure extracts compared to the other crops.

Microbial population assessment during IMO-composting production

In this study, we investigate the used of IMO produce from cooked rice in rice straw composting. The objective of this study is to identify the effect of composting using IMO and different combination of biowaste on composting of rice straw. Methodology and results: Different types of treatment were used involving rice straw and goat manure with addition or non-addition of IMO. Composting was done for 30 days in a plastic barrel and was manually turned. Temperature was measured daily while samples were analysed for moisture content, pH value and electrical conductivity (EC). Temperatures in rice straw compost contains goat manure have higher values up to 43 °C. Rice straw compost with treatment of IMO contain pro-long thermophilic phase compared to treatment without IMO. pH recorded 7.0-8.7 during the process with slight fluctuation due to the microbial activities present. EC showed higher value in rice straw compost with goat manure due to the present of soluble salt in manure. Throughout the composting time, we observed the reduction of moisture value ranging from 43% to 34%. Microbial succession in compost treated with IMO showed high population with 3.16×109 CFU/g for mesophilic microorganism during the initial phase and 7.9×108 CFU/g for thermophilic microorganism. Conclusion, significance and impact of study: Hence, it can be concluded that the IMO introduce during composting provide higher diversity of microorganisms and could pro-long the thermophilic phase, thus accelerating the process of degradation.

Interferência da incorporação de matéria orgânica no solo no controle da podridão negra da mandioca, causada por Scytalidium lignicola / Interference of incorporation of soil organic matter in control of cassava black root, caused by Scytalidium lignicola

A mandioca é de grande relevância para países do Sudeste da Ásia, África e América do Sul. Em Pernambuco as podridões vem induzindo a queda na produção e reduzindo as áreas de cultivo ao longo do tempo. O combate a essa doença ainda é um desafio, pois não existe fungicida registrado para o controle ou prevenção da doença e por ser causada por fitopatógenos habitantes do solo. Dentre a gama de fungos causadores de podridões está o Scytalidium lignicola, causador da podridão negra. Este trabalho teve como objetivo verificar o efeito de doses e fontes de matéria orgânica incorporados à solos arenosos com inoculação de Scytalidium lignicola sobre a podridão negra da mandioca cv. Pai Antônio. O experimento foi realizado com os materiais orgânicos, cama de aviário (CA) e esterco caprino (EC), incorporados ao solo arenoso nas concentrações (10, 20 e 30%) (v/v). As variáveis analisadas foram: severidade da doença, respiração basal do solo, carbono da biomassa microbiana das amostras, atributos químicos (pH, P, Na e K) e atributos bioquímicos fosfatase ácida e alcalina e urease. Os tratamentos com maiores poderes supressivos à podridão negra da mandioca foram EC 10 e 20% e CA 20 e 30%. A dose e o material orgânico que apresentou melhor desempenho sobre a supressividade da podridão negra da mandioca foi EC 10 e 20%.

Cassava is of great relevance to countries in Southeast Asia, Africa and South America In Pernambuco the rot comesin ducing drop in production and reducing the area under cultivate o nover time. Thefight against this disease remainsa challenge because there is no fungi cideregistered for the control or prevention of disease, is a disease caused by pathogen present in the soil. A mongthe range o ffungi that causerotis Scytalidium lignicola causes black rot. Thisstudy aimed to verify the effectof doses and sources of organic matterin corporated into sandy soil sinoculated with Scytalidium lignicola on the black rotof cassava cv.Father Antonio. The experimentwas conducted withorganic materials, litter (CA) andgoat manure(EC) incorporated into thesandy soilconcentrations(10, 20 and 30%) (v / v). The variables analyzed were: disease severity, soil basal respiration, microbial biomass carbonsamples, the chemical (pH, P, Na andK) and biochemical attribute sand alkaline phosphatase and urease. The suppressive treatments with greater powers to black rotof cassava were EC 10and 20%and CA20 and 30%. The dose and organic material that performed bettering the suppressiveness of cassava black root, caused by Scytalidium lignicola was EC 10 and 20%.