Simulations of surface charge density changes during the untreated solid tumour growth.

Understanding untreated tumour growth kinetics and its intrinsic behaviour is interesting and intriguing. The aim of this study is to propose an approximate analytical expression that allows us to simulate changes in surface charge density at the cancer-surrounding healthy tissue interface during the untreated solid tumour growth. For this, the Gompertz and Poisson equations are used. Simulations reveal that the unperturbed solid tumour growth is closely related to changes in the surface charge density over time between the tumour and the surrounding healthy tissue. Furthermore, the unperturbed solid tumour growth is governed by temporal changes in this surface charge density. It is concluded that results corroborate the correspondence between the electrical and physiological parameters in the untreated cancer, which may have an essential role in its growth, progression, metastasis and protection against immune system attack and anti-cancer therapies. In addition, the knowledge of surface charge density changes at the cancer-surrounding healthy tissue interface may be relevant when redesigning the molecules in chemotherapy and immunotherapy taking into account their polarities. This can also be true in the design of completely novel therapies.

Dose-response study for the highly aggressive and metastatic primary F3II mammary carcinoma under direct current.

Electrochemical treatment has been suggested as an effective alternative to local cancer therapy. Nevertheless, its effectiveness decreases when highly aggressive primary tumors are treated. The aim of this research was to understand the growth kinetics of the highly aggressive and metastatic primary F3II tumor growing in male and female BALB/c/Cenp mice under electrochemical treatment. Different amounts of electric charge (6, 9, and 18 C) were used. Two electrodes were inserted into the base, perpendicular to the tumor's long axis, keeping about 1 cm distance between them. Results have shown that the F3II tumor is highly sensitive to direct current. The overall effectiveness (complete response + partial response) of this physical agent was ≥75.0% and observed in 59.3% (16/27) of treated F3II tumors. Complete remission of treated tumors was observed in 22.2% (6/27). An unexpected result was the death of 11 direct current-treated animals (eight females and three males). It is concluded that direct current may be addressed to significantly affect highly aggressive and metastatic primary tumor growth kinetics, including the tumor complete response. Bioelectromagnetics. 39:460-475, 2018. © 2018 Wiley Periodicals, Inc.

Comparison of the Quality Adjusting of Nonlinear Models for Organs, Carcass and Body Components in Meat-Type (Coturnix Coturnix Coturnix) and Laying-Type (Coturnix Coturnix Japonica) Quail

The objective of this study was to evaluate the adjustment quality of nonlinear models to data organs growth, carcass and body components of meat-type (Coturnix coturnix coturnix) and japanese laying-type (Coturnix coturnix japonica) quail. A total of 1350 quails from one to 42 d old were distributed in a completely randomized design, with five replicates each. To determine the organs growth (gut, heart, liver and gizzard), carcass and body components (wing, thigh and drumstick, back and breast), two quails per repetition were slaughtered weekly. The data were evaluated in function of different nonlinear models (Logistical, Brody, Richards, Von Bertalanffy and Gompertz). All models studied adjusted the data, differing in adjustment quality. Brody model showed the best description of gut length to all treatments. For the data gizzard weight, heart, liver and gut, the models that best adjusted, presenting smaller residual mean square and numbers iterations were Gompertz and Logistical. The Gompertz, Logistic and Von Bertalanffy models were the most adequate to describe the thigh and drumstick growth, back and breast, and Gompertz models and Logistic to describe the wing growth and carcass, showing lesser number of iterations to achieve the convergence of date, as well as low residual mean square and squares sums of the regression residuals. The Gompertz model was the most appropriate to describe the organs growth and body components in meat- and laying-type quail when evaluated in growth phase.(AU)

Comparison of the Quality Adjusting of Nonlinear Models for Organs, Carcass and Body Components in Meat-Type (Coturnix Coturnix Coturnix) and Laying-Type (Coturnix Coturnix Japonica) Quail

The objective of this study was to evaluate the adjustment quality of nonlinear models to data organs growth, carcass and body components of meat-type (Coturnix coturnix coturnix) and japanese laying-type (Coturnix coturnix japonica) quail. A total of 1350 quails from one to 42 d old were distributed in a completely randomized design, with five replicates each. To determine the organs growth (gut, heart, liver and gizzard), carcass and body components (wing, thigh and drumstick, back and breast), two quails per repetition were slaughtered weekly. The data were evaluated in function of different nonlinear models (Logistical, Brody, Richards, Von Bertalanffy and Gompertz). All models studied adjusted the data, differing in adjustment quality. Brody model showed the best description of gut length to all treatments. For the data gizzard weight, heart, liver and gut, the models that best adjusted, presenting smaller residual mean square and numbers iterations were Gompertz and Logistical. The Gompertz, Logistic and Von Bertalanffy models were the most adequate to describe the thigh and drumstick growth, back and breast, and Gompertz models and Logistic to describe the wing growth and carcass, showing lesser number of iterations to achieve the convergence of date, as well as low residual mean square and squares sums of the regression residuals. The Gompertz model was the most appropriate to describe the organs growth and body components in meat- and laying-type quail when evaluated in growth phase.

Growth curves in meat-type and laying quail: a Bayesian perspective / Curvas de crescimento em codornas de corte e postura: uma perspectiva Bayesiana

The aim of this study was to assess the goodness of fit for nonlinear models, using the best model to describe body growth curves, comparing the parameters obtained for gender and one meat-type (Coturnix coturnix coturnix) and two laying (Coturnix coturnix japonica) quail strains, as well as nesting via MCMC (Markov chain Monte Carlo processes) methods under a Bayesian approach. A total of 1,350 one-day-old mixed quail were used: 400 of meat-type, 450 of yellow laying, and 500 of red laying strains distributed in a completely randomized design with three treatments (each treatment corresponded to one strain) and five replications. The experimental period consisted of 1 to 42 days of age. At 21 days of age, quail sexing was performed by means of sexual dimorphism, being individually identified at one day of age with numbered rings, allowing determining growth curves by gender. Birds were reared in a conventional system, fed ad libitum with diets formulated to meet nutritional requirements. Body weight was determined weekly and assessed using nonlinear models: Logistic, Brody, Von Bertalanffy, and Gompertz, whose parameters were estimated under a Bayesian approach via MCMC algorithm by means of BRugs package from the software R. DIC (Deviance Information Criterion) criterion was used to select the best nonlinear model, i.e. the lower the DIC value is, the better the model goodness of fit to thedata. Gompertz model was better adjusted to the data regardless the gender or strain. Meat-type quailhad the highest asymptotic weights and the highest age at which growth rate was maximum, followedby red and yellow strains. All nestings presented significant differences (p < 0.05) between gender forcontrasted parameters. Meat-type, yellow, and red females presented values significantly (p < 0.05)higher for asymptotic weight (370, 203, and 215 g, respectively) when compared to males (274, 131,and 143 g, respectively), which were earlier in body growth.(AU)

O objetivo deste trabalho foi avaliar a qualidade de ajuste dos modelos não lineares aos dados, utilizando o melhor modelo para descrever as curvas de crescimento corporal, comparando os parâmetros obtidos para gênero e uma linhagem de codornas de corte (Coturnix coturnix coturnix) e duas de postura (Coturnix coturnix japonica), bem como o aninhamento, via método MCMC (Cadeias de Markov em processos de Monte Carlo), sob o enfoque Bayesiano. Foram utilizadas 1.350 codornas mistas, com um dia de idade, sendo: 400 da linhagem de corte, 450 de postura amarela e 500 de postura vermelha, distribuídas por um delineamento inteiramente casualizado, com três tratamentos (cada tratamento corresponde a uma linhagem) e cinco repetições. O período experimental foi de 1 a 42 dias de idade. Aos 21 dias de idade foi realizada a sexagem por dimorfismo sexual, sendo que elas foram identificadas individualmente com um dia com anilhas numeradas, possibilitando a determinação das curvas de crescimento por gênero. As aves foram criadas em um sistema convencional, alimentadas ad libitum com rações formuladas para atender às exigências nutricionais. O peso corporal foi determinado semanalmente e avaliados por meio dos modelos não lineares: Logístico, Brody, Von Bertalanffy e Gompertz. Para a seleção domelhor modelo não linear utilizou-se o critério DIC (Deviance Information Criterion), em que quantomenor o valor de DIC, melhor é a qualidade do ajuste do modelo aos dados. Gompertz melhor se ajustouaos dados, independentemente do gênero ou linhagem. As codornas de corte tiveram os maiores pesosassintóticos e a maior idade em que a taxa de crescimento é máxima, seguido pela vermelha e amarela.Todos aninhamentos apresentaram diferenças significativas (p < 0,05) entre gênero para os parâmetroscontrastados. As fêmeas de corte, amarela e vermelha apresentaram valores significativamente (p <0,05) maiores para o peso assintótico (370, 203 e 215 g, respectivamente), comparados aos...(AU)

Growth curves in meat-type and laying quail: a Bayesian perspective / Curvas de crescimento em codornas de corte e postura: uma perspectiva Bayesiana

The aim of this study was to assess the goodness of fit for nonlinear models, using the best model to describe body growth curves, comparing the parameters obtained for gender and one meat-type (Coturnix coturnix coturnix) and two laying (Coturnix coturnix japonica) quail strains, as well as nesting via MCMC (Markov chain Monte Carlo processes) methods under a Bayesian approach. A total of 1,350 one-day-old mixed quail were used: 400 of meat-type, 450 of yellow laying, and 500 of red laying strains distributed in a completely randomized design with three treatments (each treatment corresponded to one strain) and five replications. The experimental period consisted of 1 to 42 days of age. At 21 days of age, quail sexing was performed by means of sexual dimorphism, being individually identified at one day of age with numbered rings, allowing determining growth curves by gender. Birds were reared in a conventional system, fed ad libitum with diets formulated to meet nutritional requirements. Body weight was determined weekly and assessed using nonlinear models: Logistic, Brody, Von Bertalanffy, and Gompertz, whose parameters were estimated under a Bayesian approach via MCMC algorithm by means of BRugs package from the software R. DIC (Deviance Information Criterion) criterion was used to select the best nonlinear model, i.e. the lower the DIC value is, the better the model goodness of fit to thedata. Gompertz model was better adjusted to the data regardless the gender or strain. Meat-type quailhad the highest asymptotic weights and the highest age at which growth rate was maximum, followedby red and yellow strains. All nestings presented significant differences (p < 0.05) between gender forcontrasted parameters. Meat-type, yellow, and red females presented values significantly (p < 0.05)higher for asymptotic weight (370, 203, and 215 g, respectively) when compared to males (274, 131,and 143 g, respectively), which were earlier in body growth.

O objetivo deste trabalho foi avaliar a qualidade de ajuste dos modelos não lineares aos dados, utilizando o melhor modelo para descrever as curvas de crescimento corporal, comparando os parâmetros obtidos para gênero e uma linhagem de codornas de corte (Coturnix coturnix coturnix) e duas de postura (Coturnix coturnix japonica), bem como o aninhamento, via método MCMC (Cadeias de Markov em processos de Monte Carlo), sob o enfoque Bayesiano. Foram utilizadas 1.350 codornas mistas, com um dia de idade, sendo: 400 da linhagem de corte, 450 de postura amarela e 500 de postura vermelha, distribuídas por um delineamento inteiramente casualizado, com três tratamentos (cada tratamento corresponde a uma linhagem) e cinco repetições. O período experimental foi de 1 a 42 dias de idade. Aos 21 dias de idade foi realizada a sexagem por dimorfismo sexual, sendo que elas foram identificadas individualmente com um dia com anilhas numeradas, possibilitando a determinação das curvas de crescimento por gênero. As aves foram criadas em um sistema convencional, alimentadas ad libitum com rações formuladas para atender às exigências nutricionais. O peso corporal foi determinado semanalmente e avaliados por meio dos modelos não lineares: Logístico, Brody, Von Bertalanffy e Gompertz. Para a seleção domelhor modelo não linear utilizou-se o critério DIC (Deviance Information Criterion), em que quantomenor o valor de DIC, melhor é a qualidade do ajuste do modelo aos dados. Gompertz melhor se ajustouaos dados, independentemente do gênero ou linhagem. As codornas de corte tiveram os maiores pesosassintóticos e a maior idade em que a taxa de crescimento é máxima, seguido pela vermelha e amarela.Todos aninhamentos apresentaram diferenças significativas (p < 0,05) entre gênero para os parâmetroscontrastados. As fêmeas de corte, amarela e vermelha apresentaram valores significativamente (p <0,05) maiores para o peso assintótico (370, 203 e 215 g, respectivamente), comparados aos...

Description of Aspergillus flavus growth under the influence of different factors (water activity, incubation temperature, protein and fat concentration, pH, and cinnamon essential oil concentration) by kinetic, probability of growth, and time-to-detection models.

A Box-Behnken design was used to determine the effect of protein concentration (0, 5, or 10g of casein/100g), fat (0, 3, or 6g of corn oil/100g), aw (0.900, 0.945, or 0.990), pH (3.5, 5.0, or 6.5), concentration of cinnamon essential oil (CEO, 0, 200, or 400µL/kg) and incubation temperature (15, 25, or 35°C) on the growth of Aspergillus flavus during 50days of incubation. Mold response under the evaluated conditions was modeled by the modified Gompertz equation, logistic regression, and time-to-detection model. The obtained polynomial regression models allow the significant coefficients (p<0.05) for linear, quadratic and interaction effects for the Gompertz equation's parameters to be identified, which adequately described (R2>0.967) the studied mold responses. After 50days of incubation, every tested model system was classified according to the observed response as 1 (growth) or 0 (no growth), then a binary logistic regression was utilized to model A. flavus growth interface, allowing to predict the probability of mold growth under selected combinations of tested factors. The time-to-detection model was utilized to estimate the time at which A. flavus visible growth begins. Water activity, temperature, and CEO concentration were the most important factors affecting fungal growth. It was observed that there is a range of possible combinations that may induce growth, such that incubation conditions and the amount of essential oil necessary for fungal growth inhibition strongly depend on protein and fat concentrations as well as on the pH of studied model systems. The probabilistic model and the time-to-detection models constitute another option to determine appropriate storage/processing conditions and accurately predict the probability and/or the time at which A. flavus growth occurs.

Growth curves in meat-type and laying quail: a Bayesian perspective / Curvas de crescimento em codornas de corte e postura: uma perspectiva Bayesiana

The aim of this study was to assess the goodness of fit for nonlinear models, using the best model to describe body growth curves, comparing the parameters obtained for gender and one meat-type (Coturnix coturnix coturnix) and two laying (Coturnix coturnix japonica) quail strains, as well as nesting via MCMC (Markov chain Monte Carlo processes) methods under a Bayesian approach. A total of 1,350 one-day-old mixed quail were used: 400 of meat-type, 450 of yellow laying, and 500 of red laying strains distributed in a completely randomized design with three treatments (each treatment corresponded to one strain) and five replications. The experimental period consisted of 1 to 42 days of age. At 21 days of age, quail sexing was performed by means of sexual dimorphism, being individually identified at one day of age with numbered rings, allowing determining growth curves by gender. Birds were reared in a conventional system, fed ad libitum with diets formulated to meet nutritional requirements. Body weight was determined weekly and assessed using nonlinear models: Logistic, Brody, Von Bertalanffy, and Gompertz, whose parameters were estimated under a Bayesian approach via MCMC algorithm by means of BRugs package from the software R. DIC (Deviance Information Criterion) criterion was used to select the best nonlinear model, i.e. the lower the DIC value is, the better the model goodnes

O objetivo deste trabalho foi avaliar a qualidade de ajuste dos modelos não lineares aos dados, utilizando o melhor modelo para descrever as curvas de crescimento corporal, comparando os parâmetros obtidos para gênero e uma linhagem de codornas de corte (Coturnix coturnix coturnix) e duas de postura (Coturnix coturnix japonica), bem como o aninhamento, via método MCMC (Cadeias de Markov em processos de Monte Carlo), sob o enfoque Bayesiano. Foram utilizadas 1.350 codornas mistas, com um dia de idade, sendo: 400 da linhagem de corte, 450 de postura amarela e 500 de postura vermelha, distribuídas por um delineamento inteiramente casualizado, com três tratamentos (cada tratamento corresponde a uma linhagem) e cinco repetições. O período experimental foi de 1 a 42 dias de idade. Aos 21 dias de idade foi realizada a sexagem por dimorfismo sexual, sendo que elas foram identificadas individualmente com um dia com anilhas numeradas, possibilitando a determinação das curvas de crescimento por gênero. As aves foram criadas em um sistema convencional, alimentadas ad libitum com rações formuladas para atender às exigências nutricionais. O peso corporal foi determinado semanalmente e avaliados por meio dos modelos não lineares: Logístico, Brody, Von Bertalanffy e Gompertz, cujos parâmetros foram estimados sob enfoque Bayesiano via algoritmo MCMC, utilizando o pacote BRugs do programa R. Par

Growth curves in meat-type and laying quail: a Bayesian perspective / Curvas de crescimento em codornas de corte e postura: uma perspectiva Bayesiana

The aim of this study was to assess the goodness of fit for nonlinear models, using the best model to describe body growth curves, comparing the parameters obtained for gender and one meat-type (Coturnix coturnix coturnix) and two laying (Coturnix coturnix japonica) quail strains, as well as nesting via MCMC (Markov chain Monte Carlo processes) methods under a Bayesian approach. A total of 1,350 one-day-old mixed quail were used: 400 of meat-type, 450 of yellow laying, and 500 of red laying strains distributed in a completely randomized design with three treatments (each treatment corresponded to one strain) and five replications. The experimental period consisted of 1 to 42 days of age. At 21 days of age, quail sexing was performed by means of sexual dimorphism, being individually identified at one day of age with numbered rings, allowing determining growth curves by gender. Birds were reared in a conventional system, fed ad libitum with diets formulated to meet nutritional requirements. Body weight was determined weekly and assessed using nonlinear models: Logistic, Brody, Von Bertalanffy, and Gompertz, whose parameters were estimated under a Bayesian approach via MCMC algorithm by means of BRugs package from the software R. DIC (Deviance Information Criterion) criterion was used to select the best nonlinear model, i.e. the lower the DIC value is, the better the model goodnes

O objetivo deste trabalho foi avaliar a qualidade de ajuste dos modelos não lineares aos dados, utilizando o melhor modelo para descrever as curvas de crescimento corporal, comparando os parâmetros obtidos para gênero e uma linhagem de codornas de corte (Coturnix coturnix coturnix) e duas de postura (Coturnix coturnix japonica), bem como o aninhamento, via método MCMC (Cadeias de Markov em processos de Monte Carlo), sob o enfoque Bayesiano. Foram utilizadas 1.350 codornas mistas, com um dia de idade, sendo: 400 da linhagem de corte, 450 de postura amarela e 500 de postura vermelha, distribuídas por um delineamento inteiramente casualizado, com três tratamentos (cada tratamento corresponde a uma linhagem) e cinco repetições. O período experimental foi de 1 a 42 dias de idade. Aos 21 dias de idade foi realizada a sexagem por dimorfismo sexual, sendo que elas foram identificadas individualmente com um dia com anilhas numeradas, possibilitando a determinação das curvas de crescimento por gênero. As aves foram criadas em um sistema convencional, alimentadas ad libitum com rações formuladas para atender às exigências nutricionais. O peso corporal foi determinado semanalmente e avaliados por meio dos modelos não lineares: Logístico, Brody, Von Bertalanffy e Gompertz, cujos parâmetros foram estimados sob enfoque Bayesiano via algoritmo MCMC, utilizando o pacote BRugs do programa R. Par

Effects of dietary digestible lysine levels on protein and fat deposition in the carcass of broilers

An experiment was carried out to evaluate the effects of different levels of digestible lysine in the diets of male and female broilers on protein and fat deposition. A total of 2160 Avian Farms broilers. A completely randomized experimental design was applied, and treatments consisted of the effects of three digestible lysine levels nested within each sex, with 12 replicates and 30 birds per experimental unit. The adopted digestible lysine levels corresponded to 92.5, 100.0, and 107.5% of the nutritional requirements of phases 1 to 21 days, 22 to 42 days, and 43 to 56 days of age, respectively. In each phase, the experimental diets contained similar calorie and protein levels within each sex. No significant effects of lysine levels were found on dry matter and fat percentages in the carcass of birds during the evaluated periods. Also, there were no significant effects of lysine levels on protein and fat deposition in males or females. However, males presented higher protein deposition and lower fat deposition than females during the total experimental period. Gompertz equations showed that females deposit more fat and less protein than males, and that this affected the fall in the curve of protein deposition, when the curve of fat deposition was still rising. Therefore, it was concluded that the older the broilers at slaughter, the higher their body fat content and the lower their body protein content, particularly in females.

Effects of dietary digestible lysine levels on protein and fat deposition in the carcass of broilers

An experiment was carried out to evaluate the effects of different levels of digestible lysine in the diets of male and female broilers on protein and fat deposition. A total of 2160 Avian Farms broilers. A completely randomized experimental design was applied, and treatments consisted of the effects of three digestible lysine levels nested within each sex, with 12 replicates and 30 birds per experimental unit. The adopted digestible lysine levels corresponded to 92.5, 100.0, and 107.5% of the nutritional requirements of phases 1 to 21 days, 22 to 42 days, and 43 to 56 days of age, respectively. In each phase, the experimental diets contained similar calorie and protein levels within each sex. No significant effects of lysine levels were found on dry matter and fat percentages in the carcass of birds during the evaluated periods. Also, there were no significant effects of lysine levels on protein and fat deposition in males or females. However, males presented higher protein deposition and lower fat deposition than females during the total experimental period. Gompertz equations showed that females deposit more fat and less protein than males, and that this affected the fall in the curve of protein deposition, when the curve of fat deposition was still rising. Therefore, it was concluded that the older the broilers at slaughter, the higher their body fat content and the lower their body protein content, particularly in females.