The trade-off between grain weight and grain number in wheat is explained by the overlapping of the key phases determining these major yield components.

Enhancing grain yield is a primary goal in the cultivation of major staple crops, including wheat. Recent research has focused on identifying the physiological and molecular factors that influence grain weight, a critical determinant of crop yield. However, a bottleneck has arisen due to the trade-off between grain weight and grain number, whose underlying causes remain elusive. In a novel approach, a wheat expansin gene, TaExpA6, known for its expression in root tissues, was engineered to express in the grains of the spring wheat cultivar Fielder. This modification led to increases in both grain weight and yield without adversely affecting grain number. Conversely, a triple mutant line targeting the gene TaGW2, a known negative regulator of grain weight, resulted in increased grain weight but decreased grain number, potentially offsetting yield gains. This study aimed to evaluate the two aforementioned modified wheat genotypes (TaExpA6 and TaGW2) alongside their respective wild-type counterparts. Conducted in southern Chile, the study employed a Complete Randomized Block Design with four replications, under well-managed field conditions. The primary metrics assessed were grain yield, grain number, and average grain weight per spike, along with detailed measurements of grain weight and dimensions across the spike, ovary weight at pollination (Waddington's scale 10), and post-anthesis expression levels of TaExpA6 and TaGW2. Results indicated that both the TaExpA6 and the triple mutant lines achieved significantly higher average grain weights compared to their respective wild types. Notably, the TaExpA6 line did not exhibit a reduction in grain number, thereby enhancing grain yield per spike. By contrast, the triple mutant line showed a reduced grain number per spike, with no significant change in overall yield. TaExpA6 expression peaked at 10 days after anthesis (DAA), and its effect on grain weight over the WT became apparent after 15 DAA. In contrast, TaGW2 gene disruption in the triple mutant line increased ovary size at anthesis, leading to improved grain weight above the WT from the onset of grain filling. These findings suggest that the trade-off between grain weight and number could be attributed to the overlapping of the critical periods for the determination of these traits.

Textural characteristics and abundance of microplastics in Tecolutla beach sediments, Gulf of Mexico.

Abundance of microplastics in coastal sediments is considered an emerging pollution problem worldwide. In Mexico, studies focused on microplastics in coastal sediments are little. In this study, we analyzed the textural characteristics and investigated the abundance of microplastics in the Tecolutla beach sediments, SW Gulf of Mexico. The extraction of microplastics in sediments was done by density separation. Microplastics were identified by stereomicroscope and scanning electron microscopy, and were classified based on their morphology, color, length, texture, and abundance. Among 181 microplastics, fiber type is predominant (number of microplastics n = 166; 92%) and followed by film (n = 12; 6%) and fragment (n = 3; 2%) types. The most abundant color of microplastics is black (n = 81; 45%) and second dominant color is blue (n = 65; 36%); most of them are fiber type. Based on the textural characteristics of sediments, it was observed that microplastics were derived from the fluvial contribution of the Tecolutla River and were deposited in the coastal environment by ocean currents. This study revealed that the abundance of microplastics was high in fine-grained sediments and was directly proportional to skewness. The results of this study can act as a reference for understanding the abundance of microplastics in the Gulf of Mexico coastal sediments.

Retrieval analysis of neck fracture on uni-modular total hip arthroplasty stems: The contributions of material processing and stem design.

Total hip arthroplasty stem fracture is an important contributor to morbidity rate and increases the cost of revision surgery. Failure is usually caused by issues related to overload, inadequate stem support, inappropriate stem design or dimensions and material processing. In this study, the role of the relationship between material characterization and biomechanical performance in the fracture of retrieved stems was explored. The stems were manufactured with forged stainless steel, had the same length, 12/14 trunnion, and 28-mm head. These stems were evaluated by macroscopic and microscopic examination to identify the causes of premature failure. Each stem was sectioned into four regions, and the cross-sections were used for the microhardness and grain size analysis. Finite element analysis (FEA) was carried out, considering the stem positioned at the femur, a musculoskeletal model, and biomechanical loading. All stems had fractured through a fatigue mechanism, mainly a unidirectional bending loading condition, with crack nucleation on the lateral side and propagation on the medial side. The numerical analysis revealed maximum mechanical stress on the lateral side of the stem neck, but this was below the yield stress calculated via the hardness. The use of a shorter head neck length could reduce the maximum mechanical stress at the neck. At a cross-section near the plane of the stem fracture, the hardness was lower than that normally reported by the ASM, and there were heterogonous and coarse grain sizes on the lateral side. The main cause of failure of the two stems analyzed was a combination of low hardness and coarse grain size, due to inappropriate materials processing, worsen by a high level of stress on the lateral side of the neck due to the large stem-head offset selected by the orthopedic surgeon.

Pericarp growth dynamics associate with final grain weight in wheat under contrasting plant densities and increased night temperature.

BACKGROUND AND AIMS: The pericarp weight comprises <17 % of wheat grain weight at harvest. The pericarp supports the hydration and nutrition of both the embryo and endosperm during early grain filling. However, studies of the pericarp and its association with final grain weight have been scarce. This research studied the growth dynamics of wheat pericarp from anthesis onwards and its relationship to final grain weight under contrasting plant densities and night warming. METHODS: Two spring wheat cultivars contrasting in kernel weight (Bacanora and Kambara) were sown in field conditions during seasons 2012-13 and 2014-15. Both genotypes were grown under contrasting plant density (control, 370 plants m-2; and low plant density, 44 plants m-2) and night temperatures, i.e. at ambient and increased (>6 °C) temperature for short periods before and after anthesis. From anthesis onward, grains were harvested every 3 or 4 d. Grain samples were measured and the pericarp was removed with a scalpel. Whole grain and pericarp fresh and dry weight were weighed with a precision balance. At harvest, 20 grains from ten spikes were weighed and grain dimensions were measured. KEY RESULTS: Fresh weight, dry matter and water content of pericarp dynamics showed a maximum between 110 and 235 °Cd. Maximum dry matter of the pericarp ranged between 4.3 and 5.7 mg, while water content achieved values of up to 12.5 mg. Maximum values and their timings were affected by the genotype, environmental condition and grain position. Final grain weight was closely associated with maximum dry matter and water content of the pericarp. CONCLUSIONS: Maximum pericarp weight is a determinant of grain weight and size in wheat, which is earlier than other traits considered as key determinants of grain weight during grain filling. Better growing conditions increased maximum pericarp weight, while higher temperature negatively affected this trait.

Changing nature of Saharan dust deposition in the Carpathian Basin (Central Europe): 40 years of identified North African dust events (1979-2018).

Several billion tonnes of mineral dust is emitted, and transported through winds every year from arid-semiarid areas. North African dust hot spots located in the Sahara are responsible for 50-70% of the global mineral dust budget. Dust-loaded air-masses originated from these sources can be transported over long distances and can also affect remote areas, such as North and South Americas, Europe, and the Middle East. In this study, we analysed 218 identified Saharan dust events (SDEs) in the Carpathian Basin (Central Europe) during 1979 to 2018. Systematic identification of SDEs and analyses of dust emission, dust source area activity, dust transporting wind systems, and transport routes revealed that different synoptic meteorological patterns are responsible for SDEs, and these are occurring mostly in spring and summer. The characteristic synoptic meteorological background of episodes was also identified, and three major types of atmospheric pressure-system patterns were distinguished. In recent years, several intense wintertime dust deposition events have been recorded in Central Europe. All of the identified unusual episodes were characterised by severe washout of mineral dust material and were related to very similar synoptic meteorological situations. Enhanced southward propagation of a high-latitude upper-level atmospheric trough to north-western Africa and orographic blocking of Atlas Mountains played an essential role in the formation of severe dust storms, whereas the long-range transport was associated with the northward branch of the meandering jet. The occurrence and southerly penetration of high-latitude upper-level atmospheric trough to low-latitudes and the increased meridionality of the dominant flow patterns may be associated with enhanced warming of the Arctic, leading to more meandering jet streams. Particles size of sampled dust material of some intense deposition episodes were very coarse with a considerable volumetric proportion of > 20 µm particles.

Columbus' environmental impact in the New World: Land use change in the Yaque River valley, Dominican Republic.

Columbus' arrival in the New World in AD 1492 on the northern coast of Hispaniola was followed by a suite of changes in land-use. We reconstruct environmental change from a 225-cm-long sediment core from site Los Indios from an abandoned and sediment-filled meander of the Yaque River, Cibao Valley, northeastern Dominican Republic. The sediment record starts ca. AD 195 (ca. 1755 cal. yr BP) and the history of the meander infill was monitored by changing grain size distributions, organic matter concentration and pollen from wetland plants. From ca. AD 200 to ca. AD 1525, the pollen record indicates a diverse forest assemblage; however, the presence of pollen from potential crop plants suggest nearby small-scale subsistence crop cultivation. More abundant charcoal after ca. AD 1410 shows Amerindians increasingly used fire. The record of grain size distributions shows that the meander was temporarily part of a low energetic drainage system in which bedload and suspended sediments accumulated. After European colonization of Hispaniola increasing spores of coprophilous fungi evidence that Europeans had introduced during the first decades of colonization cattle in the Cibao Valley which gradually resulted in more open forest. The charcoal record around ca. AD 1650 reflects intensive forest clearing, suggesting that small-scale Pre-Colonial practice of crop cultivation became replaced by large-scale agriculture on the moist and nutrient rich soils along the Yaque River. Further deforestation and signals of erosion suggest that the population of colonists and introduced enslaved labour force must have increased rapidly. After ca. AD 1740 charcoal influx decreased suggesting that last deforestation activities used selective cutting to produce fire wood and timber for construction, rather than burning forest in situ. Two centuries after European colonization, by the 18th century, land-use within the Cibao Valley had become a balance between substantial livestock and crop cultivation (pollen grains have evidenced cereals, maize, and potentially also sugar cane, amaranthaceous crops and tobacco). After ca. AD 1950, swamp vegetation of Typha and Cyperaceae decreased, pointing to an almost fully terrestrialized meander with only few bodies of standing water, reflecting the present-day setting. This multiproxy reconstruction of anthropogenic environmental change shows a clear differentiation between an immediate introduction of livestock and after some 150 years the development of a European style agriculture, providing a context for archaeological investigations.

Efeitos do tamanho da partícula e da concentração de sedimentos suspensos sobre a turbidez / Effect of particle size and suspended sediment concentration on turbidity

RESUMO Neste estudo foram avaliados os efeitos do tamanho da partícula e de variações da concentração de sedimentos suspensos (CSS) sobre as leituras de turbidez de três sensores óptico-nefelométricos, com iguais características ópticas e geométricas, utilizando amostras de sedimentos suspensos coletadas na seção fluviométrica da bacia do Turcato (19,5 km²), localizada na Região Sul do Brasil. O material coletado passou por processos de peneiramento e pipetagem para fracionamento nas granulometrias areia e silte. Para cada diâmetro foram separadas frações com diferentes concentrações de sedimentos, variando entre 0,01 e 5,00 g.L-1. Os resultados demonstram grande influência da CSS e do tamanho da partícula sobre a turbidez. Para qualquer situação, o aumento da turbidez é inverso ao tamanho da partícula e diretamente relacionado ao aumento da CSS. As variações da turbidez, expressas em função da CSS (sensibilidade do sensor), são maiores para menores diâmetros, atingindo valores quase constantes para partículas de areia. As leituras de turbidez dos três sensores não apresentaram diferença significativa no nível de 5% pelo teste de Mann-Whitney rank sum . Para uma mesma CSS, a leitura de turbidez pode ser diferente, conforme a classe granulométrica analisada. A turbidez registrada pelo sensor para a classe silte equivale a uma turbidez observada para uma CSS dez vezes menor para a classe das areias.

ABSTRACT This study evaluates the effects of particle size and variations of CSS on turbidity readings of three optical-nephelometric sensors using sediment samples collected in the field. Samples were collected in fluviometric station at Turcato basin (19.5 km²), located in the South region of Brazil. The collected material underwent screening and pipetting processes for fractionation in sand and silt grain size. For each diameter were separated fractions with different sediment concentration ranging between 0.01 and 5.00 g.L-1. The results demonstrate great influence of CSS and particle size on turbidity readings. The increase in turbidity is opposite to particle size and directly related to CSS. Variations in turbidity, expressed in CSS function (sensor sensitivity), are higher for smaller diameters, reaching almost constant values for sand particles. Turbidity readings of the three sensors showed no significant difference in the level of 5% by the Mann-Whitney rank sum test. For a given CSS, the turbidity reading may be different, depending on the granulometric class analyzed. Turbidity recorded by the sensor to the silt-class is equivalent to a sensor response observed for a CSS ten times lower for the sand class.

Differentially Accumulated Proteins in Coffea arabica Seeds during Perisperm Tissue Development and Their Relationship to Coffee Grain Size.

Coffee is one of the most important crops for developing countries. Coffee classification for trading is related to several factors, including grain size. Larger grains have higher market value then smaller ones. Coffee grain size is determined by the development of the perisperm, a transient tissue with a highly active metabolism, which is replaced by the endosperm during seed development. In this study, a proteomics approach was used to identify differentially accumulated proteins during perisperm development in two genotypes with regular (IPR59) and large grain sizes (IPR59-Graudo) in three developmental stages. Twenty-four spots were identified by MALDI-TOF/TOF-MS, corresponding to 15 proteins. We grouped them into categories as follows: storage (11S), methionine metabolism, cell division and elongation, metabolic processes (mainly redox), and energy. Our data enabled us to show that perisperm metabolism in IPR59 occurs at a higher rate than in IPR59-Graudo, which is supported by the accumulation of energy and detoxification-related proteins. We hypothesized that grain and fruit size divergences between the two coffee genotypes may be due to the comparatively earlier triggering of seed development processes in IPR59. We also demonstrated for the first time that the 11S protein is accumulated in the coffee perisperm.

Using Artificial Orthographies for Studying Cross-Linguistic Differences in the Cognitive and Neural Profiles of Reading.

Reading and writing are cultural inventions that have become vital skills to master in modern society. Unfortunately, writing systems are not equally learnable and many individuals struggle to become proficient readers. Languages and their writing systems often have co-varying characteristics, due to both psycholinguistic and socio-cultural forces. This makes it difficult to determine the source of cross-linguistic differences in reading and writing. Nonetheless, it is important to make progress on this issue: a more precise understanding of the factors that affect reading disparities should improve reading instruction theory and practice, and the diagnosis and treatment of reading disorders. In this review, we consider the value of artificial orthographies as a tool for unpacking the factors that create cognitive and neural differences in reading acquisition and skill. We do so by focusing on one dimension that differs among writing systems: grain size. Grain size, or the unit of spoken language that is mapped onto a visual graph, is thought to affect learning, but its impact is still not well understood. We review relevant literature about cross-linguistic writing system differences, the benefits of using artificial orthographies as a research tool, and our recent work with an artificial alphasyllabic writing system for English. We conclude that artificial orthographies can be used to elucidate cross-linguistic principles that affect reading and writing.