The structural-functional-connectivity coupling of the aging brain.

Aging primarily affects memory and executive functions, a relationship that may be underpinned by the fact that almost all adults over 60 years old develop small vessel disease (SVD). The fact that a wide range of neuropathologies could only explain up to 43% of the variation in age-related cognitive impairment suggests that other factors, such as cognitive reserve, may play a role in the brain's resilience against aging-related cognitive decline. This study aims to examine the relationship between structural-functional-connectivity coupling (SFC), and aging, cognitive abilities and reserve, and SVD-related neuropathologies using a cohort of n = 176 healthy elders from the Harvard Aging Brain Study. The SFC is a recently proposed biomarker that reflects the extent to which anatomical brain connections can predict coordinated neural activity. After controlling for the effect of age, sex, and years of education, global SFC, as well as the intra-network SFC of the dorsolateral somatomotor and dorsal attention networks, and the inter-network SFC between dorsolateral somatomotor and frontoparietal networks decreased with age. The global SFC decreased with total cognitive score. There were significant interaction effects between years of education versus white matter hyperintensities and between years of education versus cerebral microbleeds on inter-network SFC. Enlarged perivascular space in basal ganglia was associated with higher inter-network SFC. Our results suggest that cognitive ability is associated with brain coupling at the global level and cognitive reserve with brain coupling at the inter-functional-brain-cluster level with interaction effect from white matter hyperintensities and cerebral microbleed in a cohort of healthy elderlies.

Health-related physical fitness, physical activity and its correlates among school going adolescents in hilly state in north India: a cross sectional survey.

INTRODUCTION: Health-related physical fitness, which includes body composition, cardiorespiratory fitness, muscular endurance, flexibility, power, and strength are associated with risks of chronic diseases and promote good health and wellness. There have been reports of increasing levels of physical inactivity among children and adolescents, leading to increasing rates of obesity and decreased physical fitness. The present study was conducted among school going adolescents to estimate the levels and correlates of PF for timely intervention. METHODOLOGY: School based cross-sectional study was done among students of class 8-11th in Government schools of Garhwal division of Uttarakhand. Multistage stratified random sampling was applied for recruitment of study participants. We recruited a final sample size of 634 students. Validated questionnaires and standard methods for assessment of physical fitness, physical activity levels and other variables such as waist circumference, hip circumference, BMI and hemoglobin estimation were done. RESULTS: Average and above average cardiorespiratory fitness score as per Harvard step test among boys (54.3%) was significantly higher as compared to girls (21.3%) (χ2 = 88.93, p < 0.001). There was a significant association between gender and dominant handgrip strength (χ2 = 8.02, p = 0.01) as well as between gender and Shoulder stretch test (SST) of dominant (χ2 = 17.5, p < 0.05) as well as nondominant arm (χ2 = 13.5, p < 0.05). Sit and reach test results also showed a significant association with gender (χ2 = 27.17, p < 0.001). Gender, hemoglobin level, BMI and PAL scores significantly predicted cardiorespiratory fitness scores (R2 = 0.188, F value of the model = 37.69, p =< 0.001)). CONCLUSION: Physical fitness of school going adolescents in Garhwal division of Uttarakhand was better than other parts of India, with significant gender differences. Physical activity levels (PAL) were poor and are also a significant predictor of physical fitness. More emphasis needs to be paid on the health and fitness of girl students. School based policies to increase PAL among students through innovation and rewards may go a long way in improving the long-term health of the students.

Investigating the citing communities around three leading health-system frameworks.

Of numerous proposed frameworks for analyzing and impacting health systems, three stand out for the large number of publications that cite them and for their links to influential international institutions: Murray and Frenk (Bull World Health Organ 78:717-31, 2000) connected initially to the World Health Organization (WHO) and then to the Global Burden of Disease Project; Roberts et al. (Getting health reform right: a guide to improving performance and equity, Oxford University Press, Oxford, 2004) sponsored by the World Bank/Harvard Flagship Program; and de Savigny and Adam (Systems thinking for health systems strengthening, WHO, 2009) linked to the WHO and the Alliance for Health Policy and Systems Research. In this paper, we examine the citation communities that form around these works to better understand the underlying logic of these citation grouping as well as the dynamics of Global Health research on health systems. We conclude that these groupings are largely independent of one another, reflecting a range of factors including the goals of each framework and the problems that it was meant to explore, the prestige and authority of institutions and individuals associated with these frameworks, and the intellectual and geographic proximity of the citing researchers to each other and to the framework authors.

Classification of high-risk depressed mood groups in cancer patients based on Health Ecology Model.

BACKGROUND: Depressed mood affects a significant number of patients with cancer, and can impair their quality of life and interfere with successful treatment. Our study aims to create a predictive model for identifying high-risk groups of depressed mood in cancer patients, offering a theoretical support for preventing depressed mood in these individuals. METHODS: The China Health and Retirement Longitudinal Study (CHARLS) provided the data for this research, which used CES-D as a tool to identify individuals with depressed mood. Influencing factors of depressed mood in cancer patients was analyzed using a binary logistic regression model. Using the Harvard Cancer Index, we classified the high-risk patients for depressed mood. RESULTS: In present study, 52.96 % of cancer patients met criteria for depressed mood based on the CES-D. Significant correlations were found between depressed mood and factors such as gender, self-rated health, sleep duration, exercise, satisfaction with family, residence, education, life satisfaction, and medical insurance. Utilizing the Harvard Cancer Index, we classified patients into five risk levels for depressed mood, revealing a significant variation in the number of depressive patients across these levels (x2=99.82, P < 0.05). Notably, the incidence of depressed mood increased with the risk level among cancer patients (x2=103.40, P < 0.05). LIMITATIONS: Lack of data on tumor typing and subgroups makes it unlikely to explore the specifics of depressed mood in patients with various types of cancer. CONCLUSION: The determinants of depressed mood in cancer patients are multi-dimensional. The Harvard Cancer Index may be helpful in identifying high-risk populations.

John and Eliza Ware Rotch Farrar: A dual-career marriage in sickness and in health-but mostly sickness.

The story of John Farrar (1779-1853) and Eliza Ware Rotch (1791-1870) is neither a tale about a female mathematician nor one of the "making" of a mathematical career. Rather, the productivity of John, who is known to historians of American mathematics for the Harvard College series of mathematics and natural philosophy textbooks that bears his name, had already begun to decline by the time he married Eliza in 1828. She then embarked on her own career of adapting novels and biographies for children and writing self-improvement manuals. Despite their joint efforts to continue writing and to preserve their social and professional connections, the activities of both John and Eliza were effectively brought to an end by the decades John spent fully incapacitated by illness. An exploration of how their marriage was interwoven with their careers adds details to their biographies and suggests connections to the historical analysis of dual-career marriages and to examinations of how such unions can be impacted by the poor health of one or both partners.

A proposed structural connectivity matrices approach for the superior fronto-occipital fascicle in the Harvard-Oxford Atlas comparative framework following the Pandya comparative extrapolation principle.

A key set of connections necessary for the most complex brain functions are the long association cortico-cortical fiber tracts. These pathways have been described by the Dejerines and others using post mortem histological or brain dissection techniques. Given methodological limitations, these fiber connections have not been delineated completely in humans. Although the stem portions of fiber tracts have been identified in humans, their precise origins and terminations remain to be determined. By contrast, the origins and terminations as well as the stems of long cortico-cortical association fiber pathways in monkeys have been detailed in the macaque monkey brain using experimental tract tracing methods. Deepak Pandya made major contributions to the delineation of fiber tracts in the monkey brain. In the early 1990s, he compared his observations in monkeys with the original descriptions in humans by the Dejerines. With the advent of diffusion-weighted imaging, Dr. Pandya extended this line of investigation to the human brain with Dr. Nikos Makris. In this translational analysis of long association cortico-cortical fiber tracts, they applied a principle of extrapolation from monkey to human. In the present study, we addressed the reasoning and the complex methodology in translating brain structural connectivity from monkey to human in one cortico-cortical fiber tract, namely the superior fronto-occipital fascicle, which was delineated in both species by Dr. Pandya and colleagues. Furthermore, we represented this information in the form of connectional matrices in the context of the HOA2.0-ComPaRe framework, a homological monkey-to-human translational system used in neuroimaging.

Exploring the Effect of Yoga on Exercise Endurance As Assessed by Cardiorespiratory Efficiency Tests in Exercise Physiology Laboratory: A Pilot Study.

Background Today's world of cut-throat competition is boggling with stress as the most common problem among the modern generation, and reduction in stress demands a radical solution. Yoga comes as a rescuer that focuses on improving one's physical and spiritual well-being. It can increase one's strength and flexibility. Yoga practitioners have asserted the effect of physical exercise involved in it on balancing physical and spiritual health for decades, but only recently has there been a move to substantiate these claims through research. This study aimed at assessing the effect of yogic practice on exercise endurance and physical fitness as assessed by important physical fitness parameters through cardiorespiratory efficiency tests in an Exercise Physiology Laboratory. Methodology A total of 60 Bachelor of Medicine, Bachelor of Surgery (MBBS) students from a rural medical college in central India were recruited for the study. Thirty MBBS students who had undergone yogic training for six months comprised the trained or the case group, and another group of 30 students comprising the untrained group were recruited for the study from different levels of the course within the age group of 17-25 years. Body mass index (BMI) and body surface area (BSA) were calculated. Resting pulse rate and blood pressure, resting respiratory rate, maximal oxygen consumption (VO2 max), physical fitness index (PFI), breath holding time (BHT), and 40 mm Hg endurance test time was measured.  Results The mean PFI (%) in males was 88.82±5.56 and 96.05±7.44, and that in females was 82.06±8.95 and 96.55±6.47 in the control and case groups, respectively. The mean 40 mm Hg endurance test (in seconds) in males was 36.47±8.45 and 48.88±8.64 and in females was 29.79±10.30 and 38.4±10.69 in the control and test groups, respectively. The mean BHT (in seconds) in males was 44.80±14.18 and 58.91±12.35, and that in females was 42.29±15.37 and 54.60±13.36 as in control and case groups, respectively. The VO2 max evaluated by the modified Harvard step test was 2.41±0.58 L/min in control males and 3.6±0.90 L/min in the case group of males, and it was 2.14±0.49 L/min in the control group of females, and 3.76±0.69 L/min in case group of females. Conclusion By studying the dynamics of the various cardiorespiratory responses, we have determined the values of fitness parameters in the case group. It was found that the yoga group had statistically significantly higher VO2 max per minute and better PFI, BHT, and 40 mm Hg endurance values (p<0.05).

Talcott Parsons on building personality system theory via psychoanalysis.

This article examines Talcott Parsons's efforts at building the theory of personality system as a special case of his general theory of action and places those efforts in historical context. I demonstrate how, during the middle decades of the twentieth century, Parsons employed elements of classic Freudian thought to advance a new appreciation of the personality system and its relations to other action systems. I begin with an overview of the reception of psychoanalysis at the Boston Psychoanalytic Society and Institute, the Massachusetts General Hospital, and the Harvard Department of Social Relations, showing how Parsons's thinking on the personality system cannot be understood apart from his association with these three institutions. I then turn to how Parsons endeavored to integrate his particular brand of sociology with his own interpretation of Freud's writings to explain how the personality system functions and develops. I conclude by showing that while Parsons's involvements with psychoanalysis became more intermittent after the mid-1950s, to the end of his life he remained steadfast in his enthusiasm for Freud's theory of personality. In short, Parsons always believed that for sociological theory to progress, it needed to engage with psychoanalysis.

The Professors' Professor: The American Students of August Krogh.

This paper examines the creation and development of an international social network between physiologists in Denmark and the United States in the period 1907-1939. At the center of the network was the Danish physiologist and 1920 Nobel Laureate August Krogh and his Zoophysiological Laboratory at the University of Copenhagen. In total, sixteen Americans were visiting researchers at the Zoophysiological Laboratory until 1939, and more than half of them were at some point in their career affiliated with Harvard University. For many of them, their visit would be the start of a long-term connection with Krogh and the broader network. This paper shows how the American visitors, Krogh, and the Zoophysiological Laboratory benefitted from being part of this network of top researchers in physiology and medicine. The visits themselves provided the Zoophysiological Laboratory with intellectual stimulus and more manpower for its research, while the American visitors received training and developed research ideas. Beyond the visits, the network gave the members, especially the central figures such as August Krogh, access to advice, job offers, funding and travel opportunities.

A Proposed Human Structural Brain Connectivity Matrix in the Center for Morphometric Analysis Harvard-Oxford Atlas Framework: A Historical Perspective and Future Direction for Enhancing the Precision of Human Structural Connectivity with a Novel Neuroanatomical Typology.

A complete structural definition of the human nervous system must include delineation of its wiring diagram (e.g., Swanson LW. Brain architecture: understanding the basic plan, 2012). The complete formulation of the human brain circuit diagram (BCD [Front Neuroanat. 2020;14:18]) has been hampered by an inability to determine connections in their entirety (i.e., not only pathway stems but also origins and terminations). From a structural point of view, a neuroanatomic formulation of the BCD should include the origins and terminations of each fiber tract as well as the topographic course of the fiber tract in three dimensions. Classic neuroanatomical studies have provided trajectory information for pathway stems and their speculative origins and terminations [Dejerine J and Dejerine-Klumpke A. Anatomie des Centres Nerveux, 1901; Dejerine J and Dejerine-Klumpke A. Anatomie des Centres Nerveux: Méthodes générales d'étude-embryologie-histogénèse et histologie. Anatomie du cerveau, 1895; Ludwig E and Klingler J. Atlas cerebri humani, 1956; Makris N. Delineation of human association fiber pathways using histologic and magnetic resonance methodologies; 1999; Neuroimage. 1999 Jan;9(1):18-45]. We have summarized these studies previously [Neuroimage. 1999 Jan;9(1):18-45] and present them here in a macroscale-level human cerebral structural connectivity matrix. A matrix in the present context is an organizational construct that embodies anatomical knowledge about cortical areas and their connections. This is represented in relation to parcellation units according to the Harvard-Oxford Atlas neuroanatomical framework established by the Center for Morphometric Analysis at Massachusetts General Hospital in the early 2000s, which is based on the MRI volumetrics paradigm of Dr. Verne Caviness and colleagues [Brain Dev. 1999 Jul;21(5):289-95]. This is a classic connectional matrix based mainly on data predating the advent of DTI tractography, which we refer to as the "pre-DTI era" human structural connectivity matrix. In addition, we present representative examples that incorporate validated structural connectivity information from nonhuman primates and more recent information on human structural connectivity emerging from DTI tractography studies. We refer to this as the "DTI era" human structural connectivity matrix. This newer matrix represents a work in progress and is necessarily incomplete due to the lack of validated human connectivity findings on origins and terminations as well as pathway stems. Importantly, we use a neuroanatomical typology to characterize different types of connections in the human brain, which is critical for organizing the matrices and the prospective database. Although substantial in detail, the present matrices may be assumed to be only partially complete because the sources of data relating to human fiber system organization are limited largely to inferences from gross dissections of anatomic specimens or extrapolations of pathway tracing information from nonhuman primate experiments [Front Neuroanat. 2020;14:18, Front Neuroanat. 2022;16:1035420, and Brain Imaging Behav. 2021;15(3):1589-1621]. These matrices, which embody a systematic description of cerebral connectivity, can be used in cognitive and clinical studies in neuroscience and, importantly, to guide research efforts for further elucidating, validating, and completing the human BCD [Front Neuroanat. 2020;14:18].

Cross-Cultural Measurement Invariance of the Harvard Trauma Questionnaire Across Nine Adolescent Samples.

Trauma researchers often make claims about the severity of posttraumatic stress disorder (PTSD) across populations, and yet cross-cultural measurement invariance (MI) is rarely assessed. Nine youth samples with Harvard Trauma Questionnaire (HTQ) responses were grouped based on sampling strategy used into two sets: representative (Denmark, the Faroe Islands, Iceland, and Lithuania, n = 1,457), and convenience (Greenland, India, Kenya, Malaysia, and Uganda, n = 2,036). Confirmatory factor analysis (CFA) was used to gauge whether configural, metric, scalar, and residual invariance of different models held between national samples within the two sets. Configural invariance held for most PTSD models in convenience samples, not in representative samples. Metric invariance was less common, and scalar and residual in general did not hold. Cultural similarity between samples seemed to be associated with invariance. Findings suggest that although PTSD symptoms may cluster similarly across culturally distal groups, comparisons of the severity of symptoms using the HTQ across adolescent samples are not likely valid.

Evaluation of Vectra® XT 3D Surface Imaging Technology in Measuring Breast Symmetry and Breast Volume.

BACKGROUND: Breast symmetry is an essential component of breast cosmesis. The Harvard Cosmesis scale is the most widely adopted method of breast symmetry assessment. However, this scale lacks reproducibility and reliability, limiting its application in clinical practice. The VECTRA® XT 3D (VECTRA®) is a novel breast surface imaging system that, when combined with breast contour measuring software (Mirror®), aims to produce a more accurate and reproducible measurement of breast contour to aid operative planning in breast surgery. OBJECTIVES: This study aims to compare the reliability and reproducibility of subjective (Harvard Cosmesis scale) with objective (VECTRA®) symmetry assessment on the same cohort of patients. METHODS: Patients at a tertiary institution had 2D and 3D photographs of their breasts. Seven assessors scored the 2D photographs using the Harvard Cosmesis scale. Two independent assessors used Mirror® software to objectively calculate breast symmetry by analysing 3D images of the breasts. RESULTS: Intra-observer agreement ranged from none to moderate (kappa - 0.005-0.7) amongst the assessors using the Harvard Cosmesis scale. Inter-observer agreement was weak (kappa 0.078-0.454) amongst Harvard scores compared to VECTRA® measurements. Kappa values ranged 0.537-0.674 for intra-observer agreement (p < 0.001) with Root Mean Square (RMS) scores. RMS had a moderate correlation with the Harvard Cosmesis scale (rs = 0.613). Furthermore, absolute volume difference between breasts had poor correlation with RMS (R2 = 0.133). CONCLUSION: VECTRA® and Mirror® software have potential in clinical practice as objectifying breast symmetry, but in the current form, it is not an ideal test. LEVEL OF EVIDENCE IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

HOA2.0-ComPaRe: A next generation Harvard-Oxford Atlas comparative parcellation reasoning method for human and macaque individual brain parcellation and atlases of the cerebral cortex.

Comparative structural neuroanatomy is a cornerstone for understanding human brain structure and function. A parcellation framework that relates systematically to fundamental principles of histological organization is an essential step in generating structural comparisons between species. In the present investigation, we developed a comparative parcellation reasoning system (ComPaRe), which is a formal ontological system in human and non-human primate brains based on the cortical cytoarchitectonic mapping used for both species as detailed by Brodmann. ComPaRe provides a theoretical foundation for mapping neural systems in humans and other species using neuroimaging. Based on this approach, we revised the methodology of the original Harvard-Oxford Atlas (HOA) system of brain parcellation to produce a comparative framework for the human (hHOA) and the rhesus monkey (mHOA) brains, which we refer to as HOA2.0-ComPaRe. In addition, we used dedicated segmentation software in the publicly available 3D Slicer platform to parcellate an individual human and rhesus monkey brain. This method produces quantitative morphometric parcellations in the individual brains. Based on these parcellations we created a representative template and 3D brain atlas for the two species, each based on a single subject. Thus, HOA2.0-ComPaRe provides a theoretical foundation for mapping neural systems in humans and other species using neuroimaging, while also representing a significant revision of the original human and macaque monkey HOA parcellation schemas. The methodology and atlases presented here can be used in basic and clinical neuroimaging for morphometric (volumetric) analysis, further generation of atlases, as well as localization of function and structural lesions.

The Impact of Obesity on Cardiovascular Fitness in Young Individuals.

Background Young individuals are often at a higher risk for cardiovascular disease and obesity due to lifestyle changes like less physical activity and a sedentary lifestyle. Objective The aim of this study is to determine cardiovascular fitness in young individuals and to study the effects of obesity on their cardiovascular fitness. Material and methods In this study, 100 young individuals, out of which 50 were individuals with obesity and 50 were controls, including males and females, of the age group 18-25 years were included. Cardiovascular fitness was assessed in them using body mass index (BMI) and waist-to-hip ratio (WHR). Parameters like SBP (systolic blood pressure), DBP (diastolic blood pressure), PR (pulse rate), and HFI (Harvard fitness index) were measured. Results There was no difference found in the PR of the group with obesity compared to the control group (79.020/min ± 8.651 versus 79.42/min ± 6.737; p value = 0.797). However, a significant increase was observed in both SBP and DBP amongst the group with obesity compared to the control group (SBP = 122.72 mmHg ± 12.287 versus 110.92 mmHg ± 11.803; p-value < 0.001, DBP = 81.96 mmHg ± 7.913 versus 73.24 mmHg ± 11.06; p-value < 0.001). There was a significant reduction in HFI in the group with obesity than in the control group (57.44% ± 9.322 versus 80.34% ± 12.594; p-value < 0.001). When we compared males with obesity and females with obesity, we observed a non-significant difference in PR between males with obesity and females with obesity (77.12/min ± 6.02 versus 80.92/min ± 10.44; p-value = 0.122). However, we found a significant increase in SBP in males with obesity compared to females with obesity (127.76 mmHg ± 10.93 versus 117.68 mmHg ± 11.66; p-value < 0.01). A significant decrease in DBP in males with obesity (78.80 mmHg ± 7.55 versus 85.12 mmHg ± 7.07; < 0.01) than in females with obesity was also observed. Along with a non-significant increase in HFI value in males with obesity compared to females with obesity (58.96% ± 8.14 versus 55.92% ± 10.31; p-value = 0.253). Conclusion Results suggest that both male and female young individuals with obesity are at higher risk for developing cardiovascular comorbidities in the future. So, we need to focus on encouraging activities that promote physical fitness.

Containing the spread of mumps on college campuses.

College campuses are vulnerable to infectious disease outbreaks, and there is an urgent need to develop better strategies to mitigate their size and duration, particularly as educational institutions around the world adapt to in-person instruction during the COVID-19 pandemic. Towards addressing this need, we applied a stochastic compartmental model to quantify the impact of university-level responses to contain a mumps outbreak at Harvard University in 2016. We used our model to determine which containment interventions were most effective and study alternative scenarios without and with earlier interventions. This model allows for stochastic variation in small populations, missing or unobserved case data and changes in disease transmission rates post-intervention. The results suggest that control measures implemented by the University's Health Services, including rapid isolation of suspected cases, were very effective at containing the outbreak. Without those measures, the outbreak could have been four times larger. More generally, we conclude that universities should apply (i) diagnostic protocols that address false negatives from molecular tests and (ii) strict quarantine policies to contain the spread of easily transmissible infectious diseases such as mumps among their students. This modelling approach could be applied to data from other outbreaks in college campuses and similar small population settings.

Land-use history impacts spatial patterns and composition of woody plant species across a 35-hectare temperate forest plot.

Land-use history is the template upon which contemporary plant and tree populations establish and interact with one another and exerts a legacy on the structure and dynamics of species assemblages and ecosystems. We use the first census (2010-2014) of a 35-ha forest-dynamics plot at the Harvard Forest in central Massachusetts to describe the composition and structure of the woody plants in this plot, assess their spatial associations within and among the dominant species using univariate and bivariate spatial point-pattern analysis, and examine the interactions between land-use history and ecological processes. The plot includes 108,632 live stems ≥ 1 cm in diameter (2,215 individuals/ha) and 7,595 standing dead stems ≥ 5 cm in diameter. Live tree basal area averaged 42.25 m2/ha, of which 84% was represented by Tsuga canadensis (14.0 m2/ ha), Quercus rubra (northern red oak; 9.6 m2/ ha), Acer rubrum (7.2 m2/ ha) and Pinus strobus (eastern white pine; 4.4 m2/ ha). These same four species also comprised 78% of the live aboveground biomass, which averaged 245.2 Mg/ ha. Across all species and size classes, the forest contains a preponderance (> 80,000) of small stems (<10-cm diameter) that exhibit a reverse-J size distribution. Significant spatial clustering of abundant overstory species was observed at all spatial scales examined. Spatial distributions of A. rubrum and Q. rubra showed negative intraspecific correlations in diameters up to at least a 150-m spatial lag, likely indicative of crowding effects in dense forest patches following intensive past land use. Bivariate marked point-pattern analysis, showed that T. canadensis and Q. rubra diameters were negatively associated with one another, indicating resource competition for light. Distribution and abundance of the common overstory species are predicted best by soil type, tree neighborhood effects, and two aspects of land-use history: when fields were abandoned in the late 19th century and the succeeding forest types recorded in 1908. In contrast, a history of intensive logging prior to 1950 and a damaging hurricane in 1938 appear to have had little effect on the distribution and abundance of present-day tree species. Our findings suggest that current day composition and structure are still being influenced by anthropogenic disturbances that occurred over a century ago.

A pilot study adapting and validating the Harvard Trauma Questionnaire (HTQ) and PTSD checklist-5 (PCL-5) with Indian women from slums reporting gender-based violence.

BACKGROUND: Despite high rates of gender-based violence (GBV) in India, culturally sensitive measures that examine universal and culturally relevant trauma reactions are lacking. Although the Harvard Trauma Questionnaire (HTQ) has been used in India, no study has adapted the measure in full for use with this population. Similarly, the  PTSD checklist-5 (PCL-5) has not yet been validated in India. This study describes the adaptation, validation, and results from the adapted HTQ, and embedded PCL-5, for Indian women from slums reporting GBV. METHOD: This study used the adaptation framework proposed by the HTQ measure developers. The adapted HTQ contained a (1) trauma screen relevant for stressors faced by Indian women from slums, (2) description of the index trauma, (3) description of any ongoing stressors, (4) universal trauma reactions (i.e., PTSD measured by the PCL-5), and culturally relevant trauma reactions (i.e., idioms of distress measured by a scale developed for the study). This measure was piloted on 111 women from Indian slums in face-to-face interviews. Trauma characteristics, types of ongoing stressors, and psychometric properties of the PCL-5 and idioms of distress scale were explored. These scales were validated against measures of depression (PHQ-9), anxiety (GAD-7), and somatic complaints (PHQ-15). RESULTS: The majority of participants (77%) reported physical beatings, 18% reported unwanted sexual touch, and 28.8% reported infidelity as the primary emotional abuse. Further, 96.7% of GBV was perpetrated by partner or family member and over half reported ongoing stressors (e.g., poverty-related strain). The PCL-5 embedded in the HTQ yielded good internal consistency (Cronbach's alpha = .88) as did the idioms of distress scale with deletion of one item (Cronbach's alpha = .80). Both scales were externally valid, yielding large correlations with depression, anxiety, and somatic complaints (rs between .54 and .80, ps < .05). DISCUSSION: This is the first study to develop a comprehensive measure of trauma exposure with universal and culturally relevant trauma reactions in India. This study also enhances HTQ usage in India by delineating all the steps in the adaptation process. Results can inform the development of trauma-focused interventions for Indian women from slums.

Changes in ALFF and ReHo values in methamphetamine abstinent individuals based on the Harvard-Oxford atlas: A longitudinal resting-state fMRI study.

Methamphetamine (MA) abuse has become a global public health problem due to damage to various systems throughout the body, especially the central nervous system. However, the differences in resting-state brain function between short-term and long-term abstinence, the pros and cons of treatments, and the relationship between resting-state brain function and behavioral tests are unknown. Sixty-three MA abstinent individuals were followed up for nearly 1 year and treated with three different methods. The amplitude of low-frequency fluctuation (ALFF) and regional homogeneity (ReHo) based on the Harvard-Oxford atlas (HOA) were measured by resting-state functional magnetic resonance imaging (fMRI). Impulsivity was evaluated by the Barratt Impulsivity Scale-11 (BIS-11). Brain regions with significant increases in ALFF and ReHo values in the long-term abstinent group compared to the short-term abstinent group were around the right frontal pole (McKetin et al., 2012, https://doi.org/10.1111/j.1360-0443.2012.03933.x) and right middle frontal gyrus (Wang et al., 2015, https://doi.org/10.1371/journal.pone.0133431). There were no significant differences among the three groups that experienced long-term abstinence. The changes in ALFF and ReHo in the right middle frontal gyrus were significantly associated with BIS total scores, BIS attention scores, and BIS nonplanning scores. The right middle frontal gyrus is a critical region in MA long-term abstinent individuals exposed to therapeutic intervention, and this region may be useful, when combined with BIS-11, as a potential biomarker to identify the effect of abstinence with therapeutic intervention in MA individuals.

Correlation between physical efficiency index using Harvard step test and heart rate variation in college students.

The purpose of this study is to investigate the relationship between physical efficiency index (PEI) calculated by the Harvard step test and heart rate variation (HRV), and to identify parameters of HRV that can predict PEI in college students. Sixteen college students were participated in this study and they were randomly divided into two groups; higher PEI group (HPEI, n=6) and lower PEI group (LPEI, n=10). To investigate the relationship between PEI and HRV, we were measured HRV and Harvard step test. HRV test was the resting, immediately, 15 min and 30 min after the Harvard step test using electrocardiography device polyG-I. Relationship between PEI and HRV were determined Pearson correlation coefficient, and multiple regression analysis was performed for examining HRV parameters to predict PEI. As shown in the result, not only PEI was negatively correlated with root mean squared differences between adjacent normal RR intervals (RMSSD), but had a positive correlation with low-frequency/high-frequency, but also normalized low frequency (normLF), the ratio of LF, and RMSSD, the change in RR interval showed a significant difference at each time point of measurement according to PEI levels. But, there were no significant differences among the HRV variables except normLF and RMS-SD. Our findings suggest a critical information that PEI calculated by the Harvard step test can be used as an index to predict the autonomic nerve function, and high PEI may have a positive effect on changes in autonomic nerve activity during recovery after exercise intervention.