Can we redevelop ammonia nitrogen contaminated sites without remediation? The key role of subsurface pH in human health risk assessment.

Nitrogen fertilizer supports global food production, but its manufacturing results in substantial ammonia nitrogen (AN) contaminated sites which remain largely unexplored. In this study, ten representative AN contaminated sites were investigated, covering a wide range of subsurface pH, temperature, and AN concentration. A total of 7232 soil samples and 392 groundwater samples were collected to determine the concentration levels, migration patterns, and accurate health risks of AN. The results indicated that AN concentrations in soil and groundwater reached 12700 mg/kg and 12600 mg/L, respectively. AN concentrations were higher in production areas than in non-production areas, and tended to migrate downward from surface to deeper soil. Conventional risk assessment based on AN concentration identified seven out of the ten sites presenting unacceptable risks, with remediation costs and CO2 emissions amounting to $1.67 million and 17553.7 tons, respectively. A novel risk assessment model was developed, which calculated risks based on multiplying AN concentration by a coefficient fNH3 (the ratio of NH3 to AN concentration). The mean fNH3 values, primarily affected by subsurface pH, varied between 0.02 and 0.25 across the ten sites. This new model suggested all investigated sites posed acceptable health risks related to AN exposure, leading to their redevelopment without AN-specific remediation. This research offers a thorough insight into AN contaminated site, holds great realistic significance in alleviating global economic and climate pressures, and highlights the need for future research on refined health risk assessments for more contaminants.

Eyelid Lesion of Molluscum contagiosum: A Case Report and Literature Review.

Molluscum contagiosum (MC) is a common viral infection in children that affects the skin and oral mucous membranes. It is caused by the molluscum contagiosum virus (MCV), a double-stranded DNA virus in the Poxviridae family. Transmission takes place via direct skin contact, self-inoculation, and exposure to contaminated objects. Clinically, it is characterized by the presence of a single or multiple enlarged dome-shaped or doughnut-shaped flesh-colored papules with central umbilication, usually called "mollusca". The diagnosis of MC is based mainly on clinical observations, in addition to histopathological examinations to reveal characteristic molluscum bodies, also known as Henderson-Patterson bodies. Current treatment methods include mechanical, chemical, immune modulation, and antiviral treatments. In this context, we present a case involving a 42-year-old male infected with MC, outlining both the clinical and histopathological findings.

Application of Virginia Henderson's Theory Within an Intimate Partner Violence Scenario.

The practice column focuses on incorporating a nursing theoretical framework into nursing care in a clinical scenario involving intimate partner violence. Accordingly, as exemplified by utilizing Virginia Henderson's theory, there is guidance to define, focus, and evaluate nursing care in these multifaceted clinical cases.

Thomas Shields Henderson - Brooklyn Dentist and Irish Nationalist.

An unusual card advertising the dental services of T.S. Henderson revives the story of an Irish dentist who left his homeland and came to Brooklyn, New York to practice. He was a fervent Irish Nationalist and was active in Irish causes. Henderson was an abuser of alcohol and eventually found dead in Albany, New York. The death was considered a suicide, but was it?

Virginia Henderson's Writings on the Nature of Nursing: An Exemplar of Nursing Practice.

Virginia Henderson's views on the nature of nursing continues to serve patients. Henderson emphasized that with the increasing complexity and technology in healthcare, nursing has never had a more important opportunity to contribute to the placement of the patient in the best conditions to achieve health. The aim of this article is to highlight a case study that employed Henderson's principles and plan of care to assist a child with a diagnosis of hemolytic uremic syndrome (HUS) through the performance of activities centering on achieving health and recovery.

Holistic care of patients with diabetic foot ulcers during the COVID-19 era: integration of Henderson's Need Theory.

The COVID-19 pandemic has inhibited the practice of diabetic foot ulcer care, particularly in the community. Comprehensive theory-based nursing care is needed to prevent further complications. Unfortunately, a study combining theory with nursing care in diabetic foot ulcer care has not been explored. When caring for patients with diabetic foot ulcers, who are also at increased risk of severe complications from COVID-19, it is important to take a holistic view of the patient and consider all of their needs and the factors affecting them. Henderson's Need Theory and the 14 basic needs contained within it was chosen to be integrated in the care of patients with diabetic foot ulcers during the pandemic, with the hope that the findings will help nurses to optimise care in both hospital-based and community practice.

Describing acid-base balance using three different methods of analysis in a feline acute haemorrhage-resuscitation model.

OBJECTIVE: To describe acid-base status using the Henderson-Hasselbalch, Stewart and semi-quantitative methods of analysis in a feline haemorrhage-resuscitation model. STUDY DESIGN: Randomized crossover study. ANIMALS: A total of six domestic cats (mean age, 21 months; weight, 4.9 kg). METHODS: Venous blood samples were taken before haemorrhage, after haemorrhage at 30 minute intervals during fluid resuscitation and at 24 hours. The cats were anaesthetized and underwent following treatments: no purposeful haemorrhage and resuscitation (NoPHR), purposeful haemorrhage followed by either lactated Ringer's solution (LRS) or 6% tetrastarch 130/0.4 (Voluven) for resuscitation. LRS and Voluven were administered at 60 and 20 mL kg-1 hour-1, respectively, for 120 minutes. Variables used for the analysis methods were measured or calculated from the blood samples and then compared among treatments over time using a general linear mixed model (p < 0.05; data reported as mean and standard deviation). RESULTS: The total blood loss at 120 minutes was 10.2 ± 2.3, 29.3 ± 9.0 and 29.1 ± 6.3 mL kg-1 for NoPHR, LRS and Voluven, respectively. Total volumes of LRS and Voluven administered were 120 and 40 mL kg-1, respectively. All cats became acidaemic during anaesthesia regardless of treatment. The Henderson-Hasselbalch method indicated that anaesthetized cats undergoing severe haemorrhage and resuscitation manifest a mixed acidosis. The Stewart method indicated two counter metabolic processes that contributed to the overall pH-decrease in apparent strong ion difference (acidosis) and decrease in total weak acids (alkalosis). The semi-quantitative method identified the free water and chloride effects as variables causing acidosis and the albumin effect causing alkalosis. CONCLUSIONS AND CLINICAL RELEVANCE: In an experimental haemorrhage and resuscitation model in cats, blood pH was similar among treatments over time regardless of severe haemorrhage and resuscitation with LRS or Voluven or mild haemorrhage and no resuscitation.

Mathematical Description of the Enzymatic Activity of Proteins with Ionizable Groups Exhibiting Deviations from the Henderson-Hasselbalch Equation.

The ionization equilibrium implied in the calculation of the specific activity is classically described through the Henderson-Hasselbalch equation. An extension for the description of anomalous ionization profiles using the Hill equation is presented in this communication. The proposed framework was applied to the description of the specific enzymatic activity curve as a function of pH of five enzymes presenting different ionization states in their active site. The developed equation improves the description of relative enzymatic curves that deviate from the bell curve predicted by the application of the Henderson-Hasselbalch equation, regardless of the ionization scheme related to the active site.

Melanocytic marker Melan-A detects molluscum contagiosum bodies.

Melan-A is one of the most commonly used immunohistochemical assays (IHC) in dermatopathology laboratories to detect the presence and outline the distribution of melanocytes. It is a cytoplasmic stain that detects a melanocyte-specific cytoplasmic protein involved in the formation of stage II melanosomes. Clinically, Melan-A is primarily used to detect and confirm melanocytic tumors although it is also positively expressed in adrenal cortical tumors and sex cord stromal tumors. We found that Melan-A also detected and highlighted Henderson-Patterson bodies of molluscum poxvirus. To determine if other melanocytic markers detect molluscum contagiosum bodies, S-100, HMB-45, MITF, and SOX-10 were also tested. In 15 tested molluscum cases, Melan-A stains were positive in all cases, whereas the other tested melanocytic markers were negative. Our results confirm that Melan-A is very sensitive in detecting molluscum contagiosum bodies and could be clinically useful to supplement the hematoxylin and eosin (H&E) in cases that are very inflamed or only have limited biopsy material.

Pectus excavatum en adolescentes: cuidados de Enfermería en el postoperatorio / Pectus excavatum in adolescents: nursing care during the postoperative period

El pectus excavatum es una deformidad congénita de la pared torácica caracterizada por el hundimiento del esternón. Esta alteración, aparte de ser una cuestión estética, conlleva un impacto psicosocial en el individuo. Se presenta el caso de un varón de 16 años sometido a intervención quirúrgica de pectus excavatum. Se realizó una valoración según las 14 Necesidades de Virginia Henderson y se definió un plan de cuidados individualizado empleando la taxonomía diagnóstica de la North American Nursing Diagnosis Association (NANDA), describiendo los resultados esperados según la Nursing Outcomes Classification (NOC) y enunciado las intervenciones necesarias según la Nursing Intervention Classification (NIC). Se identificaron seis diagnósticos enfermeros NANDA: “[00118] Trastorno de la imagen corporal”, “[00120] Baja autoestima situacional”, “[00053] Aislamiento social”, “[00121] Trastorno de la identidad personal”, “[00132] Dolor agudo”, “[00046] Deterioro de la integridad tisular”. Se identificaron 11 resultados esperados NOC y ocho intervenciones NIC con sus correspondientes actividades enfermeras. La evaluación del plan de cuidados mostró que se alcanzaron las puntuaciones diana de los indicadores de los NOC a excepción de los indicadores “[150303] Interacción con miembros de la familia” y “[150307] Participación en organización de actividades” que se mantuvieron. Además, en algunos como ”[120507] Comunicación abierta” y “[120014] Adaptación a cambios corporales por cirugía” se superó la puntuación diana. En conclusión, el paciente mostró un mayor grado de aceptación de su imagen corporal, y recuperó progresivamente el estado de ánimo y la relación con sus iguales.(AU)

Pectus excavatum is a congenital deformity of the thoracic wall characterized by a caved-in sternum. This alteration, besides being an aesthetic issue, entails a psychosocial impact on the individual. We present the case of a 16-year-old male patient undergoing surgery for pectus excavatum. An assessment was conducted according to Virginia Henderson’s 14 Needs, and an individualized plan of care was defined, using the diagnostic taxonomy by the North American Nursing Diagnosis Association (NANDA), describing the expected outcomes according to the Nursing Outcomes Classification (NOC), and stating the interventions required according to the Nursing Intervention Classification (NIC). Six NANDA nursing diagnoses were identified: “[00118] Body Image Disorder”, “[00120] Situational Low Self-Esteem”, “[00053] Social Isolation”, “[00121] Disturbed Personal Identity”, “[00132] Acute Pain”, “[00046] Impaitment of Skin Integrity”. Eleven (11) expected NOC outcomes were identified, as well as eigh NIC interventions with their relevant nursing activities. The assessment of the plan of care showed that the target scores for the NOC indicatiors were reached, except for “[150303] Interacts with Family Members” and “[150307] Participates in Organized Activities”, which were sustained. Besides, the target score was exceeded in some indicators such as ”[120507] Open Communication” and “[120014] Adjustment to Body Changes due to Surgery”. In conclusion, patients showed a higher level of acceptance of their body image, and recovered gradually their mood and relationship with peers.(AU)

Bioelectrical signal associated with sweet taste transduction in humans is a hyperpolarizing potential on the lingual epithelium.

The lingual surface potential (LSP), which hyperpolarizes in response to salt and bitter stimuli, is thought to be a bioelectrical signal associated with taste transduction in humans. In contrast, a recent study reported sweet and sour stimuli to evoke a depolarization of the LSP. We questioned the origin of such a depolarization because liquid junction potentials (JPs), which arise at the interfaces of recording electrode and taste solutions, are neglected in the report. We recorded the LSPs to sucrose and NaCl solutions on the human tongue using an Ag/AgCl electrode. To estimate JPs generated by each taste solution, we made an agar model to simulate the human tongue. The lingual surface was rinsed with a 10 mM NaCl solution that mimics the sodium content of the lingual fluid. In the human tongue, sucrose dissolved in distilled water evoked a depolarizing LSP that could be attributed to JPs, resulting from the change in electrolyte concentration of the taste solution. Sucrose dissolved in 10 mM NaCl solution evoked a hyperpolarizing LSP which became more negative in a concentration-dependent manner (300-1500 mM). Lactisole (3.75 mM), an inhibitor of sweet taste, significantly reduced the LSPs and decreased perceived intensity of sweetness by human subjects. The negative JPs generated by 100 mM NaCl in the agar model were not different from the LSPs to 100 mM NaCl. When the electrolyte environment on the lingual surface is controlled for JPs, the bioelectrical signal associated with sweet taste transduction is a hyperpolarizing potential.

Crosstalk of physiological pH and chemical pKa under the umbrella of physiologically based pharmacokinetic modeling of drug absorption, distribution, metabolism, excretion, and toxicity.

Introduction: Physiological pH and chemical pKa are two sides of the same coin in defining the ionization of a drug in the human body. The Henderson-Hasselbalch equation and pH-partition hypothesis form the theoretical base to define the impact of pH-pKa crosstalk on drug ionization and thence its absorption, distribution, metabolism, excretion, and toxicity (ADMET).Areas covered: Human physiological pH is not constant, but a diverse, dynamic state regulated by various biological mechanisms, while the chemical pKa is generally a constant defining the acidic dissociation of the drug at various environmental pH. Works on pH-pKa crosstalk are scattered in the literature, despite its significant contributions to drug pharmacokinetics, pharmacodynamics, safety, and toxicity. In particular, its impacts on drug ADMET have not been effectively linked to the physiologically based pharmacokinetic (PBPK) modeling and simulation, a powerful tool increasingly used in model-informed drug development (MIDD).Expert opinion: Lacking a full consideration of the interactions of physiological pH and chemical pKa in a PBPK model limits scientists' capability in mechanistically describing the drug ADMET. This mini-review compiled literature knowledge on pH-pKa crosstalk and its impacts on drug ADMET, from the viewpoint of PBPK modeling, to pave the way to a systematic incorporation of pH-pKa crosstalk into PBPK modeling and simulation.

Iniciativas solidarias para ancianos confinados por pandemia de COVID-19: Análisis de contenido temático en prensa / Iniciativas de solidariedade para idosos confinadas pela pandemia de COVID-19: Análise de conteúdo temático na imprensa / Solidarity initiatives for the elderly confined by the COVID-19 pandemic: Thematic content analysis in press

Introducción: El confinamiento derivado del Estado de Alarma ante la pandemiapor COVID-19 dejó a los ancianos solos en una situación de mayor vulnerabilidad.Objetivo: Describir las iniciativas solidarias hacia los ancianos en la prensa escrita en elcontexto del confinamiento en España por Coronavirus. Material y métodos: Estudiocualitativo mediante análisis de contenido temático. Se empleó el buscador Google paralocalizar artículos en medios de divulgación periodística en formato digital del ámbitoespañol (local, regional o nacional), publicados entre el 14 de marzo y el 8 de mayo de2020. Realizamos una ficha en Excel con: título, fuente, ámbito, fecha, quincena, sección,autor, verbatín, agentes implicados, medio de contacto, necesidad implicada segúncategorización de Virginia Henderson y dirección web. Resultados: De los 100 artículosanalizados, la mayoría se publicaron en la primera quincena de confinamiento en ámbitolocal. Cubrían necesidades como "detectar y evitar peligros", "alimentación","comunicación", "movilización" y "aprendizaje". Los agentes implicados fueron lasociedad civil, también ONGs e instituciones públicas mediante Protección Civil o CruzRoja. Los medios empleados de comunicación fueron carteles, teléfono, teleasistencia y mail. Conclusiones: La pandemia del coronavirus ha desencadenado una red desolidaridad hacia los ancianos y la prensa se ha hecho eco de ello.(AU)

Introduction: The confinement derived from the Alarm State in the face of theCOVID-19 pandemic left the elderly alone in a situation of greater vulnerability.Objective: To describe the solidarity initiatives towards the elderly in the written press inthe context of confinement in Spain by Coronavirus. Material and methods: Qualitativestudy through thematic content analysis. The Google search engine was used to locatearticles in journalistic dissemination media in digital format of the Spanish scope (local,regional or national), published between March 14 and May 8, 2020. We made an Excelfile with: title, source, scope, date, fortnight, section, author, verbatim, agents involved,means of contact, necessity implied according to Virginia Henderson's categorization andweb address.Results: Of the 100 articles analyzed, most were published in the first fortnight ofconfinement at the local level. They covered needs such as "detect and avoid dangers","food", "communication", "mobilization" and "learning". The agents involved were civilsociety, also NGOs and public institutions through Civil Protection or the Red Cross. Themeans of communication used were posters, telephone, telecare and mail. Conclusions:The coronavirus pandemic has unleashed a network of solidarity towards the elderly andthe press has echoed this.(AU)

Introdução: O confinamento derivado do Estado de Alarme diante da pandemia deCOVID-19 deixou os idosos sozinhos em uma situação de maior vulnerabilidade.Objetivo: Descreva as iniciativas de solidariedade para idosos na imprensa escrita nocontexto do confinamento na Espanha por Coronavírus. Material e métodos: Estudoqualitativo através da análise de conteúdo temática. O mecanismo de busca do Google foiusado para localizar artigos em mídia de divulgação jornalística em formato digital doescopo em espanhol (local, regional ou nacional), publicado entre 14 de março e 8 demaio de 2020. Criamos um arquivo em Excel com: title, fonte, escopo, data, quinzena,seção, autor, literalmente, agentes envolvidos, meios de contato, necessidade envolvidade acordo com a categorização e o endereço da web de Virginia Henderson. Resultados:Dos 100 artigos analisados, a maioria foi publicada na primeira quinzena de confinamentoem nível local. Eles cobriram necessidades como "detectar e evitar perigos", "comida","comunicação", "mobilização" e "aprendizado". Os agentes envolvidos eram a sociedade civil, também ONGs e instituições públicas através da Proteção Civil ou da CruzVermelha. Os meios de comunicação utilizados foram cartazes, telefone, telecare ecorreio. Conclusões: A pandemia de coronavírus desencadeou uma rede de solidariedadepara com os idosos e a imprensa ecoou isso.(AU)

Ionizable Drug Self-Associations and the Solubility Dependence on pH: Detection of Aggregates in Saturated Solutions Using Mass Spectrometry (ESI-Q-TOF-MS/MS).

It is widely accepted that solubility-pH profiles of ionizable compounds follow the Henderson-Hasselbalch equation. However, several studies point out that compounds often undergo additional processes in saturated solutions, such as sub-micellar oligomerization, micellar aggregation, or drug-buffer complexation among others, which make the experimental profiles deviate from the behavior predicted by the Henderson-Hasselbalch equation. Often, the presence of additional processes is supported by the analysis of experimental data through solubility computer programs. However, the purpose of this work is to experimentally prove the aggregation phenomena for a series of bases for which deviations from the theoretical profile have been observed. To this end, five monoprotic bases (lidocaine, maprotiline, cyproheptadine, bupivacaine, and mifepristone) susceptible to form ionic aggregates in solution have been selected, and mass spectrometry has been the technique of choice to prove the presence of aggregation. High declustering potentials have been applied to prevent aggregates from forming in the ionization source of the mass spectrometer. In addition, haloperidol has been used as a negative control since according to its profile, it is not suspected to form ionic aggregates. In all instances, except for haloperidol, the analysis of the saturated solutions revealed the presence of mixed-charged dimers (aggregates formed by a neutral molecule and a charged one) and even trimers in the case of mifepristone and bupivacaine. For lidocaine, the most soluble of the compounds, the presence of neutral aggregates was also detected. These experiments support the hypothesis that the simple Henderson-Hasselbalch equation may explain the solubility-pH behavior of certain compounds, but it can be somewhat inaccurate in describing the behavior of many other substances.

Assessing plastic size distribution and quantity on a remote island in the South Pacific.

Plastics are an environmental threat; however, their fate once in the pelagic environment is poorly known. We compare results from assessments of floating plastics in the South Pacific Ocean with accumulated beach plastics from Henderson Island. We also compare accumulated plastic mass on Henderson during 2015 and 2019 and investigate the presence of nanoplastics. There were differences between the size classes of beach and pelagic plastics, and an increase in microplastics (0.33-5 mm) on the beach between 2015 and 2019. Micro- and nanoplastics were found at all sites (mean ± SE: 1960 ± 356 pieces/kg dw). Across the whole beach this translates to >4 billion plastic particles in the upper 5 cm. This is concerning, particularly given Henderson is uninhabited and distant from urban centres (~2350 km from Pape'ete, French Polynesia). The vast number of small particles on Henderson may make nearshore filter feeders susceptible to ingestion and subsequent detrimental impacts.

Jackson-Henderson technique for the revision surgery following PDA rupture in a dog.

A 3-month-old female intact Maltese dog, was referred for further investigation and management of a patent ductus arteriosus (PDA), which was diagnosed with a grade V murmur during thoracic auscultation and a palpable thrill in the left third intercostal space during routine vaccination. Echocardiographic findings included left ventricle dilatation, high velocity continuous ductal flow in the pulmonary valve and a patent ductus arteriosus. Hematological and biochemical abnormalities were not detected. A left forth intercostal thoracotomy was performed and during dissection, the medial wall of the duct was perforated resulting in hemorrhage. Digital pressure was applied over the ductus and bleeding ceased. An attempt for further dissection aggravated bleeding so it was decided to abandon surgery and to reoperate the dog using a different technique. Three months after surgery a second procedure was scheduled. A Jackson-Henderson technique was chosen for the ductus ligation, which was completed through a left fourth intercostal thoracotomy and the dog was discharged 2 days postoperatively. The dog was reexamined at 2 and 8 months after surgery and found with no clinical evidence of heart disease. In conclusion the Jackson-Henderson technique was used in the second surgery for closure of the ductus in order to avoid dissection of the medial aspect of the ductus, where adhesions were formed and the dog was free of clinical signs of heart disease 2 and 8 months postoperatively.

Describing precisely what nurses do.

Numerous important papers written by nurses and other scientists to improve nursing practice are not read by many nurses because two of the common ways authors use to describe what nurses do obscures the applicability of studies to nurses in general. Interventions (aka, procedures, skills, tasks) used and populations studied, including diseases, are less robust indicators of research results than are tests of nursing theory. Further, some of these important papers are not stored in or retrieved by accessing the Cumulative Index of Nursing and Allied Health Literature database. We believe many research papers by nurses and those who study nursing would benefit from an explicit rather than implicit test of nursing theory and we advise authors use Henderson's theoretical textbook because of its link to research and expert opinion professional literature. Significant papers should be reversely cited in her textbook to place them in the context of the knowledge of nursing she recorded for much of the 20th century.

Quantum mechanical reference spectrum simulation for precursors and degradation products of chemicals relevant to the Chemical Weapons Convention.

A selection of acidic, alkaline and neutral degradation products relevant to the Chemical Weapons Convention was studied in wide range of pH conditions to determine their spin systems as well as spectral parameters. The pH dependence of chemical shifts and J couplings was parameterized using Henderson-Hasselbalch-based functions using dichloromethane as additional shift reference in TSP-d4 referenced spectra. The resulting parameters allowed calculation of precise chemical shifts and J coupling constants in arbitrary pH conditions. The validity of the obtained spin system definitions and parameters as a source of quantum mechanically simulated reference data in chemical verification analysis is demonstrated.

The Acid-Base Equilibrium of Pyrazinoic Acid Drives the pH Dependence of Pyrazinamide-Induced Mycobacterium tuberculosis Growth Inhibition.

Pyrazinamide, a first-line antibiotic used against Mycobacterium tuberculosis, has been shown to act in a pH-dependent manner in vitro. Why pyrazinamide, an antitubercle prodrug discovered more than 65 years ago, exhibits this pH-dependent activity was unclear. Upon entering mycobacterial cells, pyrazinamide is deamidated to pyrazinoate by an enzymatic process and exists in an acid-base equilibrium with pyrazinoic acid. Thus, the effects of total pyrazinoic acid (pyrazinoic acid + pyrazinoate) on M. tuberculosis growth, pH homeostasis, and proton motive force over a range of pH values found in host tissues were investigated. Although M. tuberculosis was able to maintain pH homeostasis over an external pH range of 7.0 to 5.5, total pyrazinoic acid induced growth inhibition increased as culture medium pH was decreased from 7.3 to 6.4. Consistent with growth inhibition, total pyrazinoic acid increased both acidification of the bacterial cytoplasm and dissipation of membrane potential as the environmental pH decreased when added to the bacterial suspensions. The results suggest pyrazinoic acid is the active form of the drug, which acts as an uncoupler of proton motive force, likely a protonophore, providing a mechanistic explanation for the pH dependence of the drug activity.