Negative cross-resistance to clomazone in imazethapyr-resistant Echinochloa crus-galli caused by increased metabolization.

Herbicide resistance is frequently reported in E. crus-galli globally with target and non-target site resistance mechanism to acetolactate synthase (ALS)-inhibiting herbicides. However, resistance to certain herbicides can result in increased sensitivity to other herbicides, a phenomenon called negative cross-resistance. The objective of this study is to identify the occurrence of negative cross-resistance (NCR) to the pro-herbicide clomazone in populations of E. crus-galli resistant to ALS inhibitors due to increased metabolization. Clomazone dose-response curves, with and without malathion, were performed in imazethapyr-resistant and -susceptible E. crus-galli biotypes. CYPs genes expression and antioxidant enzymes activity were also evaluated. The effective dose to reduce 50% (ED50) of dry shoot weight obtained in the clomazone dose-response curves of the metabolic based imazethapyr-resistant and -susceptible biotypes groups were 22.712 and 58.745 g ha-1, respectively, resulting in a resistance factor (RF) of 0.37, indicating the occurrence of NCR. The application of malathion prior to clomazone increased the resistance factor from 0.60 to 1.05, which indicate the reversion of the NCR. Some CYP genes evaluated were expressed in a higher level, ranging from 2.6-9.1 times according to the biotype and the gene, in the imazethapyr-resistant than in -susceptible biotypes following clomazone application. Antioxidant enzyme activity was not associated with NCR. This study is the first report of NCR directly related to the mechanism of resistance increased metabolization in plants. The occurrence of NCR to clomazone in E. crus-galli can help delay the evolution of herbicide resistance.

Influence of soil properties and soil moisture on the efficacy of indaziflam and flumioxazin on Kochia scoparia L.

BACKGROUND: Kochia (Kochia scoparia L.) is a highly competitive, non-native weed found throughout the western United States. Flumioxazin and indaziflam are two broad-spectrum pre-emergence herbicides that can control kochia in a variety of crop and non-crop situations; however, under dry conditions, these herbicides sometimes fail to control this important weed. There is very little information describing the effect of soil properties and soil moisture on the efficacy of these herbicides. RESULTS: Soil organic matter (SOM) explained the highest proportion of variability in predicting the herbicide dose required for 80% kochia growth reduction (GR80 ) for flumioxazin and indaziflam (R2 = 0.72 and 0.79 respectively). SOM had a greater impact on flumioxazin phytotoxicity compared to indaziflam. Flumioxazin and indaziflam kochia phytotoxicity was greatly reduced at soil water potentials below -200 kPa. CONCLUSION: Kochia can germinate at soil moisture potentials below the moisture required for flumioxazin and indaziflam activation, which means that kochia control is greatly influenced by the complex interaction between soil physical properties and soil moisture. This research can be used to gain a better understanding of how and why some weeds, like kochia, are so difficult to manage even with herbicides that normally provide excellent control. © 2016 Society of Chemical Industry.