ABSTRACT
Objective: To establish the HPLC method for determination of oxysophocarpine (OSC) and optimize the extraction and purification technology of OSC from Sophora alopecuroides by inverse phase membrane. Methods: Based on single-factor test, the influence of aqueous phase and organic phase volume ratio, the concentration of sodium hydroxide and hydrochloric acid, and the extraction cycle time were investigated using orthogonal design method. Results: OSC was determined by Shim-pack VP-ODS chromatographic column (250 mm×4.6 mm, 5 μm), mobile phase was methanol-0.2% phosphoric acid aqueous solution (7:93), gradient elution, flow rate was 1 mL/min, column temperature was 30℃, and detection wavelength was 221 nm. The ratio of aqueous phase and organic phase volume was 1:1, hydrochloric acid concentration was 0.03 mol/L, sodium hydroxide concentration was 0.5 mol/L, water pump flow rate was 6 mL/min, and cycle time was 60 min. The extraction rate of OSC 98.21% in 60 min was under the best experimental conditions. OSC had good linearity relationship within the range of 0.01-0.7 mg/mL, r2=0.9978, and the respective average recovery rate was 97.47%, RSD=1.95%. Conclusion: This extraction technology is simple operation, with low organic solvent consumption, and can be used for alkaloids extraction.
