ABSTRACT
OBJECTIVE:To study the effects of LYS polysaccharides on learning & memory and monoamine neurotrans-mitter content in SAMP8 brain.METHODS:50 6-month-old SAMP8 mice were randomized to 5 groups(10 in each group):SAMP8 group,huperzine A control group,low-,mid-and high-dose groups of polysaccharides.Another 10 6-month-old SAMR1 mice were assigned to normal control group.After medication for 40 days,the learning and memory abilities of mice in each group were detected with Morris water maze method.The norepinephrine(NE),dopamine(DA) and 5-HT contents in brain were determined by HPLC.RESULTS:The learning and memory abilities of SAMP8 group decreased significantly,and the NE,DA and 5-HT contents in brain decreased significantly compared with normal group(P
ABSTRACT
Aim To observe the effects of LYS polysaccharides on expressions of APP, PS1, PS2 and ApoE in SAMP8 mouse brain. Methods 50 6-month-old SAMP8 mice were used and divided randomly into 5 groups: SAMP8 untreated control group, huperzine A control group, low-, mid-and high-dose groups of polysaccharides, with 10 mice in each group. 10 6-month-old SAMR1 mice were used as normal control. After each group was treated by corresponding drug for 40 days continuously, the mRNA contents of APP, PS1, PS2 and ApoE in brain tissue were assayed by reverse transcription polymerase chain reaction.Results The low-, mid-and high-dose groups of polysaccharides could reduce the mRNA contents of APP, PS1 and PS2, which showed a dose-effect relationship in some degrees. But it could not affect the content of ApoE. Conclusion LYS polysaccharides could inhibit the expressions of APP, PS1, and PS2 in SAMP8 mouse brain.