Taxonomic notes on the collection of the tribe Lamiini (Coleoptera, Cerambycidae) housed in the Natural History Museum, London.

In the present paper, the genus Mimomyagrus Breuning, 1970 is synonymized with Combe Thomson, 1864, and Mimomyagruspfanneri Breuning, 1970 is considered a junior synonym of Combebrianus (White, 1858). The female of Arctolamiasinica Bi & Chen, 2022 is described for the first time and this species is reported as new to Myanmar. Type material of Lamiapunctator Fabricius, 1776 [= Anoplophorachinensis (Forster, 1771)], Cerambyxgalloprovincialis Olivier, 1800 [= Monochamusgalloprovincialis (Olivier, 1800)] and Melanaustergranulipennis Breuning, 1938 [= Monochamusguerryi Pic, 1903] are confirmed to be preserved in Natural History Museum, London.

At a crossroads: Genetic lineages and dispersal routes of Morimusasper (Sulzer, 1776) s.l. (Coleoptera, Cerambycidae) in Bulgaria.

The present study fills a knowledge gap in the distribution and genetic variation of Morimus populations in the Balkans, by studiyng the representatives of the genus in Bulgaria - M.asperfunereus Mulsant, 1862, M.verecundusbulgaricus Danilevsky, 2016 and M.orientalis Reitter, 1894. Additional information is provided for Albania and northern Greece. The mitochondrial cytochrome C oxidase subunit I (COI) marker and the nuclear internal transcribed spacer 2 (ITS2) were used for the genetic analyses. Three of the previously-defined mitochondrial lineages (Lb/HgA, L2 and L3) were detected in Bulgaria, as well as a new lineage (Str) from the Strandzha Mountains (south-eastern Bulgaria). A total of 24 distinct haplotypes, 20 of them in Bulgaria, were found. Bulgarian populations of Morimus demonstrated relatively high nucleotide diversity. The L3 COI lineage was confirmed as the most diverse and frequent in the Balkans. The L3 lineage is dominant in most of Bulgaria, but was not identified in the easternmost parts near the Black Sea coast, where the L2 and Str lineages were found. New data highlighted two dispersal routes of the L2 mitochondrial lineage on the Balkan Peninsula: 1) northwards along the Black Sea coast and 2) westwards, across the Balkans where only disjunct populations remain. North-western Bulgaria seems to be the eastern limit of the basal lineage Lb/HgA distribution. Our results show high levels of genetic exchange between most of the mitochondrially defined lineages, yet some of the easternmost populations probably remained isolated for comparatively longer periods.

First evidence of a horizontally-acquired GH-7 cellobiohydrolase from a longhorned beetle genome.

Xylophagous larvae of longhorned beetles (Coleoptera; Cerambycidae) efficiently break down polysaccharides of the plant cell wall, which make the bulk of their food, using a range of carbohydrate-active enzymes (CAZymes). In this study, we investigated the function and evolutionary history of the first identified example of insect-encoded members of glycoside hydrolase family 7 (GH7) derived from the Lamiinae Exocentrus adspersus. The genome of this beetle contained two genes encoding GH7 proteins located in tandem and flanked by transposable elements. Phylogenetic analysis revealed that the GH7 sequences of E. adspersus were closely related to those of Ascomycete fungi, suggesting that they were acquired through horizontal gene transfer (HGT) from fungi. However, they were more distantly related to those encoded by genomes of Crustacea and of protist symbionts of termites and cockroaches, supporting that the same enzyme family was recruited several times independently in Metazoa during the course of their evolution. The recombinant E. adspersus GH7 was found to primarily break down cellulose polysaccharides into cellobiose, indicating that it is a cellobiohydrolase, and could also use smaller cellulose oligomers as substrates. Additionally, the cellobiohydrolase activity was boosted by the presence of calcium chloride. Our findings suggest that the combination of GH7 cellobiohydrolases with other previously characterized endo-ß-1,4-glucanases and ß-glucosidases allows longhorned beetles like E. adspersus to efficiently break down cellulose into monomeric glucose.

The collection of the genus Epepeotes Pascoe, 1866 housed in the Natural History Museum, London (Coleoptera, Cerambycidae).

Data on the collection of the genus Epepeotes Pascoe, 1866 deposited in the Natural History Museum, London are presented. A total of 23 species/subspecies, including type specimens of 18 names, of them 13 valid, are recorded. Epepeotesuncinatuslineatopunctatus Breuning, 1960 is restored to subspecies-level status. Lectotypes are designated for Epepeotesuncinatusuncinatus Gahan, 1888 and Epepeotesandamanicus Gahan, 1893. Epepeotesluscusluscus (Fabricius, 1787) is newly recorded in Cambodia and Singapore, and Epepeotesuncinatusuncinatus Gahan, 1888 is newly recorded in Bangladesh. Images of the type and other significant specimens are provided for 23 taxa, mainly for the first time.

Duplication of horizontally acquired GH5_2 enzymes played a central role in the evolution of longhorned beetles.

The rise of functional diversity through gene duplication contributed to the adaption of organisms to various environments. Here we investigate the evolution of putative cellulases of the subfamily 2 of glycoside hydrolase family 5 (GH5_2) in the Cerambycidae (longhorned beetles), a megadiverse assemblage of mostly xylophagous beetles. Cerambycidae originally acquired GH5_2 from a bacterial donor through horizontal gene transfer (HGT), and extant species harbor multiple copies that arose from gene duplication. We ask how these digestive enzymes contributed to the ability of these beetles to feed on wood. We analyzed 113 GH5_2, including the functional characterization of 52 of them, derived from 25 species covering most subfamilies of Cerambycidae. Ancestral gene duplications led to five well-defined groups with distinct substrate specificity, allowing these beetles to break down, in addition to cellulose, polysaccharides that are abundant in plant cell walls (PCWs), namely, xyloglucan, xylan, and mannans. Resurrecting the ancestral enzyme originally acquired by HGT, we show it was a cellulase that was able to break down glucomannan and xylan. Finally, recent gene duplications further expanded the catalytic repertoire of cerambycid GH5_2, giving rise to enzymes that favor transglycosylation over hydrolysis. We suggest that HGT and gene duplication, which shaped the evolution of GH5_2, played a central role in the ability of cerambycid beetles to use a PCW-rich diet and may have contributed to their successful radiation.

The Effect of Trap Color on Catches of Monochamus galloprovincialis and Three Most Numerous Non-Target Insect Species.

Black pheromone-baited traps are commonly used for monitoring Monochamus galloprovincialis, a vector of Bursaphelenchus xylophilus, although few studies have been conducted on its response to color (black, white, and clear). The objective of our studies was to evaluate the attractiveness of different colors to M. galloprovincialis and non-target species: Spondylis buprestoides and predatory Thanasimus formicarius and T. femoralis. Laboratory tests of fifteen colors against immature and mature M. galloprovincialis revealed some differences in their color preference. In two field tests, eight colors of coroplast vanes in cross-vane traps were compared with unpainted white (a reference (RF)). The first test confirmed the laboratory results, i.e., RF was slightly more attractive to M. galloprovincialis than pastel yellow, reseda green, and cyan blue, but trap color had no significant effect on any of the insect species studied. In the second test, the attractiveness of RF was highest and significantly different from pure white (for all four species), light blue, and pine green (except S. buprestoides). Overall, the unpainted white traps appeared to be most effective in catching M. galloprovincialis. Thanasimus spp. responded to the colors similarly to M. galloprovincialis; therefore, either trap design or lure composition should be modified to reduce their catches.

A revision of the genus Cylindroeme Vives (Coleoptera, Cerambycidae, Cerambycinae).

The genus Cylindroeme Vives, 2019 is taxonomically revised. The type species, Cylindroemevietnamica Vives, 2019 is redescribed and a second species from Qinghai (China), Cylindroemeshii sp. nov., is described and illustrated. The genus is redescribed and a key to the known species is presented. The genus is also recorded from China for the first time.

Taxonomic study on the genus Xenicotela Bates from China (Cerambycidae, Lamiinae, Lamiini).

A taxonomic review of the Chinese species of the genus Xenicotela Bates, 1884 is presented. A new species, Xenicotelagriseomaculata sp. nov., is described from Chongqing, China, and a new combination, Xenicotelaconvexicollis (Gressitt, 1942) comb. nov., is proposed.

3-Hydroxyhexan-2-one and 3-Methylthiopropan-1-ol as Pheromone Candidates for the South American Cerambycid Beetles Stizocera phtisica and Chydarteres dimidiatus dimidiatus, and Six Related Species.

Here, we study the pheromone chemistry of two South American cerambycid beetle species, and their behavioral responses to candidate pheromone components. Adult males of Stizocera phtisica Gounelle (subfamily Cerambycinae: tribe Elaphidiini) produced a sex-specific blend of (R)-3-hydroxyhexan-2-one with lesser amounts of 3-methylthiopropan-1-ol. In field bioassays, traps baited with racemic 3-hydroxyhexan-2-one and 3-methylthiopropan-1-ol did not catch conspecific beetles, but did catch both sexes of a sympatric species, Chydarteres dimidiatus dimidiatus (F.) (Cerambycinae: Trachyderini). We found that males of this species also produce (R)-3-hydroxyhexan-2-one and 3-methylthiopropan-1-ol, and small amounts of 2-phenylethanol. Subsequent bioassays with these compounds showed that a blend of 3-hydroxyhexan-2-one and 3-methylthiopropan-1-ol constitutes the aggregation-sex pheromone of C. d. dimidiatus, with 2-phenylethanol not influencing the attraction of conspecifics. During the field bioassays, six other species in the Cerambycinae also were caught in significant numbers, including Aglaoschema ventrale (Germar) (tribe Compsocerini), congeners Chrysoprasis aurigena (Germar), Chrysoprasis linearis Bates, and an unidentified Chrysoprasis species (Dichophyiini), and Cotyclytus curvatus (Germar) and Itaclytus olivaceus (Laporte & Gory) (both Clytini), suggesting that one or more of the compounds tested are also pheromone components for these species.

Species survey of the subfamily Cerambycinae (Coleoptera: Cerambycidae) in the Rio Doce State Park, Minas Gerais, Brazil.

The Rio Doce State Park ("PERD") is the largest Atlantic Forest remnant in Minas Gerais State, Brazil, with predominantly semi-deciduous forests. The longhorned beetles of the Cerambycinae subfamily (Coleoptera: Cerambycidae) are distributed worldwide, developing on healthy, stressed or recently dead trees. Faunistic surveys are necessary to understand about the Minas Gerais' cerambycid fauna due to a lack of research, especially in the eastern region of the state. A list of species of the subfamily Cerambycinae (Coleoptera: Cerambycidae), collected in the PERD during the rainy season (September 2013- February 2014), is presented. The beetles were collected using a light trap and through their emergence from Anadenanthera colubrina (Vell.) Brenan (Fabaceae) logs. A total of 663 individuals of 33 species, 30 genera, and 15 tribes of the Cerambycinae subfamily were collected. The species Malacopterus tenellus (Fabricius, 1801) was registered for the first time in Minas Gerais State, while A. colubrina is a new host plant for 14 Cerambycinae species. The geographical distribution, number of host plants and materials examined are presented for each species collected. This is the first list of Cerambycidae species from the Rio Doce State Park.

A new species of Miccolamia Bates, 1884 (Coleoptera: Cerambycidae: Lamiinae) from northeast India, with comments on related species.

A new species of Cerambycidae, Miccolamia (Miccolamia) arunachalensis Sreedevi Ghate sp. nov. (Lamiinae: Desmiphorini) is described from northeast India. This is the fourth species of Miccolamia from India. Additionally, an updated key to the Indian species is also provided along with comments on related species of the subgenus.

Description of two new Acanthoderini, and description of the male of Oreodera advena Martins amp; Galileo (Coleoptera, Cerambycidae, Lamiinae).

Two new species of Acanthoderini are described: Aegomorphus antonkozlovi, from Peru; and Plistonax antonkozlovi, from Panama. The male of Oreodera advena Martins Galileo, 2005 is described for the first time, and a new Colombian department record is provided.

On the enigmatic genus Oectropsis Blanchard (Coleoptera, Cerambycidae, Lamiinae): tribal allocation, synonymy, and new species.

The species of the genus Oectropsis Blanchard, 1851 (Cerambycidae, Lamiinae) are exclusively distributed west of the central Andes region (Chile) and were probably isolated due to phenomena caused by the Andes Mountains. Recent taxonomic revisions of the genus revealed that the species do not have the main features of Acanthocinini, the tribe in which this genus is currently allocated. Instead, they share characteristics with Pogonocherini, especially Pogonocherus Dejean, 1821, whose majority of species are distributed in North America. In addition to the tribal transference, we describe a new species, update a previous key, and we discuss the taxonomic status of some tribes.

Molecular phylogenetic assessment of the tribal classification of Lamiinae (Coleoptera: Cerambycidae).

Lamiinae is the most diverse subfamily of longhorned beetles, with about 20,000 described species classified into 80 tribes. Most of the tribes of Lamiinae were proposed during the 19th century and the suprageneric classification of the subfamily has never been assessed under phylogenetic criteria. In this study, we present the first tribal-level phylogeny of Lamiinae, inferred from 130 terminals (representing 46 tribes, prioritizing generic type species of the tribes) and fragments of two mitochondrial and three nuclear markers (cox1, rrnL, Wg, CPS and LSU; 5,024 aligned positions in total). Analyses were performed under Maximum Likelihood and Bayesian methods based on two datasets: a dataset including all taxa available for the study, and a reduced dataset with 111 terminals where taxa only contributing with mitochondrial markers were excluded from the matrix. The monophyly of Lamiinae was corroborated in three of the four analyses and 11 of the 35 tribes with more than one species represented in the analyses were consistently recovered as monophyletic. However, 15 tribes were not retrieved as monophyletic, requiring a revision of their boundaries: Acanthocinini, Acanthoderini, Agapanthiini, Apomecynini, Desmiphorini, Dorcaschematini, Enicodini, Hemilophini, Monochamini, Onciderini, Parmenini, Phytoeciini, Pogonocherini, Pteropliini and Saperdini. Based on these results, when strong support values for paraphyly were recovered, we argue a number of tribe synonymies, including Moneilemini as synonym of Acanthocinini; Onocephalini of Onciderini; Dorcadionini, Gnomini, Monochamini and Rhodopinini of Lamiini; and Obereini and Phytoeciini of Saperdini. Other taxonomic changes proposed in this study based on the criterion of monophyly and supported by morphological characters include the transfer of Tricondyloides and Stenellipsis to Enicodini, and of Dylobolus stat. rest., which is removed as subgenus of Mecas and restituted as genus, to Hemilophini. Furthermore, our analyses suggest that Ostedes and Neohoplonotus should be removed from Acanthocinini and Parmenini, respectively, and Colobotheini should be redefined to encompass several genera currently placed in Acanthocinini.

New species and new combination in the genus Plistonax Thomson, 1864 (Cerambycidae, Lamiinae, Acanthoderini).

Plistonax Thomson, 1864 is a Neotropical genus of longhorned beetles (Cerambycidae: Lamiinae) currently composed of six species. Herein, we describe a new species (Plistonax albituberculatus Silva Júnior Souza sp. nov.) from Brazilian States of Rio de Janeiro and Bahia. We also propose the transference of Exalphus hefferni Audureau, 2017 to Plistonax, resulting in the following comb. nov.: Plistonax hefferni (Audureau, 2017).

Notes on Onciderini (Coleoptera, Cerambycidae, Lamiinae): description of a new species of Lesbates Dillon amp; Dillon and new distribution records.

Lesbates chavesi sp. nov. is described and a key to species of the genus is presented. In addition, nine new records of Onciderini for the states of Rio de Janeiro and Paraná (Brazil) are provided.

A new genus and species of Oemini (Coleoptera, Cerambycidae) from Sichuan Province, western China.

A new genus and species of Oemini (Oemina), Sichuanortia zhouchaoi, is described from Sichuan Province, western China. The new genus is similar to the genus Oemospila Gahan, 1906 in general appearance, but it is clearly distinguished by the deeply furrowed gula instead of the relatively smooth gula as in the latter.

Species of Lamiinae (Insecta, Coleoptera, Cerambycidae) from east Paraná State (Brazil), with new geographic records.

A list of species of Lamiinae (Cerambycidae) collected in Iguaçu National Park (PNI) during the period from March 2012 to December 2015, using different collection methods, is provided. A total of 134 species were sampled, all recorded for the first time in the PNI and including 38 new distribution records for the state of Paraná, and one new record for Brazil. The geographic distribution and material examined are stated for each species. Each new record also accompanied by an illustration of the species.

Notes on the biology and distribution of the tribe Agallissini in North America (Coleoptera: Cerambycidae: Cerambycinae).

New distributional records, new larval host records, various collecting notes, and observations are reported for the North American species of the tribe Agallissini LeConte, 1873 (Coleoptera: Cerambycidae: Cerambycinae): Agallissus lepturoides (Duponchel Chevrolat, 1841), Osmopleura chamaeropis (Horn, 1893), and Zagymnus clerinus (LeConte, 1873). The species are illustrated and distribution maps are provided.

Descriptions, redescription, notes, and new ranks in American Cerambycidae (Coleoptera).

Four new species of Lamiinae are described and illustrated: Estoloides jaliscana (Lamiinae, Desmiphorini), from Mexico (Jalisco); Allotigrinestola nuevoleonis (Desmiphorini), from Mexico (Nuevo Léon); Pogonillus panamensis (Desmiphorini), from Panama; and Phrynidius nayaritensis (Lamiinae, Apomecynini), from Mexico (Nayarit). Drycothaea stictica stictica Bates, 1881 (Lamiinae, Calliini) is redescribed, and notes on it and Drycothaea stictica lepidiota Bates, 1885 are provided. Ornithia mexicana mexicana (Sturm, 1843) (Cerambycinae, Dryobiini) and O. m. zapotensis Tippmann, 1960 are considered distinct species.