Pangenome and multi-tissue gene atlas provide new insights into the domestication and highland adaptation of yaks.

BACKGROUND: The genetic diversity of yak, a key domestic animal on the Qinghai-Tibetan Plateau (QTP), is a vital resource for domestication and breeding efforts. This study presents the first yak pangenome obtained through the de novo assembly of 16 yak genomes. RESULTS: We discovered 290 Mb of nonreference sequences and 504 new genes. Our pangenome-wide presence and absence variation (PAV) analysis revealed 5,120 PAV-related genes, highlighting a wide range of variety-specific genes and genes with varying frequencies across yak populations. Principal component analysis (PCA) based on binary gene PAV data classified yaks into three new groups: wild, domestic, and Jinchuan. Moreover, we proposed a 'two-haplotype genomic hybridization model' for understanding the hybridization patterns among breeds by integrating gene frequency, heterozygosity, and gene PAV data. A gene PAV-GWAS identified a novel gene (BosGru3G009179) that may be associated with the multirib trait in Jinchuan yaks. Furthermore, an integrated transcriptome and pangenome analysis highlighted the significant differences in the expression of core genes and the mutational burden of differentially expressed genes between yaks from high and low altitudes. Transcriptome analysis across multiple species revealed that yaks have the most unique differentially expressed mRNAs and lncRNAs (between high- and low-altitude regions), especially in the heart and lungs, when comparing high- and low-altitude adaptations. CONCLUSIONS: The yak pangenome offers a comprehensive resource and new insights for functional genomic studies, supporting future biological research and breeding strategies.

An Optimized Instance Segmentation of Underlying Surface in Low-Altitude TIR Sensing Images for Enhancing the Calculation of LSTs.

The calculation of land surface temperatures (LSTs) via low-altitude thermal infrared remote (TIR) sensing images at a block scale is gaining attention. However, the accurate calculation of LSTs requires a precise determination of the range of various underlying surfaces in the TIR images, and existing approaches face challenges in effectively segmenting the underlying surfaces in the TIR images. To address this challenge, this study proposes a deep learning (DL) methodology to complete the instance segmentation and quantification of underlying surfaces through the low-altitude TIR image dataset. Mask region-based convolutional neural networks were utilized for pixel-level classification and segmentation with an image dataset of 1350 annotated TIR images of an urban rail transit hub with a complex distribution of underlying surfaces. Subsequently, the hyper-parameters and architecture were optimized for the precise classification of the underlying surfaces. The algorithms were validated using 150 new TIR images, and four evaluation indictors demonstrated that the optimized algorithm outperformed the other algorithms. High-quality segmented masks of the underlying surfaces were generated, and the area of each instance was obtained by counting the true-positive pixels with values of 1. This research promotes the accurate calculation of LSTs based on the low-altitude TIR sensing images.

Efficient Vertical Structure Correlation and Power Line Inference.

High-resolution three-dimensional data from sensors such as LiDAR are sufficient to find power line towers and poles but do not reliably map relatively thin power lines. In addition, repeated detections of the same object can lead to confusion while data gaps ignore known obstacles. The slow or failed detection of low-salience vertical obstacles and associated wires is one of today's leading causes of fatal helicopter accidents. This article presents a method to efficiently correlate vertical structure observations with existing databases and infer the presence of power lines. The method uses a spatial hash key which compares an observed tower location to potential existing tower locations using nested hash tables. When an observed tower is in the vicinity of an existing entry, the method correlates or distinguishes objects based on height and position. When applied to Delaware's Digital Obstacle File, the average horizontal uncertainty decreased from 206 to 56 ft. The power line presence is inferred by automatically comparing the proportional spacing, height, and angle of tower sets based on the more accurate database. Over 87% of electrical transmission towers were correctly identified with no false negatives.

High Altitude Head and Neck Paragangliomas: A First Sub-Himalayan Experience.

Objectives: High-altitude natives have a high incidence of parangangliomas (PGL) of the head and neck, especially the carotid body tumor. The aim of this study is to describe the clinical presentation, pattern, altitude of residence, distribution, management, and follow-up of head and neck paragangliomas (HNPGL) in our sub-Himalayan population. Study Design: Retrospective cohort study. Setting: Academic tertiary care hospital. Methods: Hospital records of 20 patients of HNPGL diagnosed from December 2017 to December 2021 were retrieved for analysis. Results: Twenty patients with 23 HNPGL, with a mean age of 41.74 years were managed in our institute. The female-to-male ratio was 2.3: 1 and the mean follow-up was 29.95 months. Nine had carotid body (CBPGL), 7 had tympanic (TPGL), 2 had jugular (JPGL), and 2 had vagal paragangliomas (VPGL). Multiple PGL were seen in 4 patients (20%). Majority of cases (all CBPGL and 57.14% of TPGL) were residents of the high altitude, and the rest were from the low altitude. Fifteen patients (8 CBPGL, 7 TPGL) were operated. There were no major complications except in a patient with large carotid body tumor required anastomosis of carotid artery. Five patients received stereotactic radiotherapy, and 1 malignant PGL received chemoradiotherapy. Conclusion: In this study, JPGL and VPGL are common at low altitudes, whereas carotid body and tympanic PGL were the most common tumor at high altitudes. Being a retrospective and study small sample size, a definite conclusion is not established, however, a genetic analysis and inclusion of a wider population in a future prospective study may establish the hypothesis.

Research on Classification of Grassland Degeneration Indicator Objects Based on UAV Hyperspectral Remote Sensing and 3D_RNet-O Model.

Real-time and high-precision land cover classification is the foundation for efficient and quantitative research on grassland degradation using remote sensing techniques. In view of the shortcomings of manual surveying and satellite remote sensing, this study focuses on the identification and classification of grass species indicating grassland degradation. We constructed a UAV-based hyperspectral remote sensing system and collected field data in grassland areas. By applying artificial intelligence technology, we developed a 3D_RNet-O model based on convolutional neural networks, effectively addressing technical challenges in hyperspectral remote sensing identification and classification of grassland degradation indicators, such as low reflectance of vegetation, flat spectral curves, and sparse distribution. The results showed that the model achieved a classification accuracy of 99.05% by optimizing hyperparameter combinations based on improving residual block structures. The establishment of the UAV-based hyperspectral remote sensing system and the proposed 3D_RNet-O classification model provide possibilities for further research on low-altitude hyperspectral remote sensing in grassland ecology.

A comparison of quality of life between older adults living in high and low altitude areas.

Background: High altitude is known to have a significant impact on human physiology and health, therefore, understanding its relationship with quality of life is an important research area. This study compared the quality of life (QOL) in older adults living in high and low altitude areas, and examined the independent correlates of QOL in those living in a high altitude area. Methods: Older adults living in three public nursing homes in Xining (high altitude area) and one public nursing home in Guangzhou (low altitude area) were recruited. The WHOQOL-BREF was used to measure the QOL. Results: 644 older adults (male: 39.1%) were included, with 207 living in high altitude and 437 living in low altitude areas. After controlling for the covariates, older adults living in the high altitude area had higher QOL in terms of physical (P = 0.035) and social domains (P = 0.002), but had lower QOL in psychological (P = 0.009) domain compared to their counterparts living in the low altitude area. For older adults living in the high altitude area, smoking status was associated with higher social QOL (P = 0.021), good financial status was associated with higher physical QOL (P = 0.035), and fair or good health status was associated with higher physical (p < 0.001) and psychological QOL (P = 0.046), while more severe depressive symptoms were associated with lower QOL. Conclusion: Appropriate interventions and support to improve depressive symptoms and both financial and health status should be developed for older adults living in high altitude areas to improve their QOL.

A Real-Time Path Planning Method for Urban Low-Altitude Logistics UAVs.

To solve the problem of poor real-time performance in path planning algorithms for unmanned aerial vehicles (UAVs) in low-altitude urban logistics, a path planning method combining modified Beetle Antennae Search (BAS) with the Simulated Annealing (SA) algorithm is proposed. Firstly, based on the requirements of task execution and constraints of UAV flight, a fitness function for real-time search of waypoints is designed while ensuring the safety and obstacle avoidance of the UAV. Then, to improve the search accuracy and real-time performance, determining the initial search direction in the BAS algorithm is improved, while the search step size and antennae sensing length are updated in real-time according to the distance between the UAV and the obstacle. Finally, the SA algorithm is combined with the BAS algorithm to update the waypoints, expanding the search range of each waypoint, avoiding the process of updating the waypoints from becoming trapped in the local optimal waypoints. Meanwhile, the effectiveness of the next waypoint is evaluated based on the Metropolis criterion. This paper generates a virtual urban logistics distribution environment based on the density and distribution of urban buildings, and compares the performance of algorithms in obstacle-sparse, obstacle-moderate, and obstacle-dense environments. The simulation results demonstrate that the improved method in this paper has a more significant capacity for environmental adaptation. In terms of the path length, waypoints, safety obstacle avoidance, and smoothness, the planned path outperforms the original BAS method. It satisfies the needs of real-time path planning for UAVs involved in urban low-altitude logistics.

Diverse diets and low-fiber, low-tannin foraging preferences: Foraging criteria of Tibetan macaques (Macaca thibetana) at low altitude in Huangshan.

Nutrient composition and food availability determine food choices and foraging strategies of animals, while altitude and geographical location affect species distribution and food availability. Tibetan macaques (Macaca thibetana) have sophisticated foraging strategies as the largest species in Macaca. They are important in understanding the ecological evolution of the entire genus. However, the mechanism of food selection in Tibetan macaques at low altitudes remains unclear. In this study, we researched a wild Tibetan macaques group (Tianhu Mountain Group, 29 individuals) living in a low-altitude area around Mt. Huangshan, Anhui Province, China. We used instantaneous scan sampling to observe these macaques' foraging behavior from September 2020 to August 2021. We recorded the dietary composition and food availability, compared the nutrient content of staple food and non-food items, and analyzed the role of key nutrients in food selection. We found that Tibetan macaques forage on 111 plants belonging to 93 genera and 55 families. The food types included fruits (52.5%), mature leaves (17.0%), bamboo shoots (14.4%), young leaves (6.3%), flowers (4.5%), others (2.1%), stems (1.9%), and tender shoots (1.3%). Tibetan macaques forage for a maximum of 76 plant species during spring. However, dietary diversity was highest during summer (H' = 3.052). Monthly fruit consumption was positively correlated with food availability. Staple foods are lower in fiber, tannin, and water than non-foods. In addition, the time spent foraging for specific foods was negatively correlated with the fiber and tannin content of the food. The results showed that Tibetan macaques' foraging plant species and food types were diverse, and their foraging strategies varied seasonally. Our findings confirmed the effect of nutrients on food choice in Tibetan macaques. We highlighted the important role of fiber and tannin in their food choices and suggested that the foraging behavior of Tibetan macaques is highly flexible and adaptive.

Low-Altitude Infrared Slow-Moving Small Target Detection via Spatial-Temporal Features Measure.

Robust detection of infrared slow-moving small targets is crucial in infrared search and tracking (IRST) applications such as infrared guidance and low-altitude security; however, existing methods easily cause missed detection and false alarms when detecting infrared small targets in complex low-altitude scenes. In this article, a new low-altitude slow-moving small target detection algorithm based on spatial-temporal features measure (STFM) is proposed. First, we construct a circular kernel to calculate the local grayscale difference (LGD) in a single image, which is essential to suppress low-frequency background and irregular edges in the spatial domain. Then, a short-term energy aggregation (SEA) mechanism with the accumulation of the moving target energy in multiple successive frames is proposed to enhance the dim target. Next, the spatial-temporal saliency map (STSM) is obtained by integrating the two above operations, and the candidate targets are segmented using an adaptive threshold mechanism from STSM. Finally, a long-term trajectory continuity (LTC) measurement is designed to confirm the real target and further eliminate false alarms. The SEA and LTC modules exploit the local inconsistency and the trajectory continuity of the moving small target in the temporal domain, respectively. Experimental results on six infrared image sequences containing different low-altitude scenes demonstrate the effectiveness of the proposed method, which performs better than the existing state-of-the-art methods.

The DOA Estimation Method for Low-Altitude Targets under the Background of Impulse Noise.

Due to the discontinuity of ocean waves and mountains, there are often multipath propagation effects and obvious pulse characteristics in low-altitude detection. If the conventional direction of arrival (DOA) estimation method is directly used for direction finding, it will lead to a large error. In view of serious misalignment in the DOA estimation of multipath signals under the background of impulse noise, a DOA estimation method based on spatial difference and a modified projection subspace algorithm is proposed in this paper. Firstly, the covariance matrix of the received data vector is used for spatial difference to eliminate the multipath effects of low-altitude targets. Secondly, the modified projection matrix is constructed using the signal source estimated with the least squares criterion and then used for modifying the covariance matrix, thus eliminating the cross-covariance matrices that affect the estimation accuracy. Finally, the modified covariance matrix is used for the DOA estimation of targets. Simulations show that the proposed algorithm achieves a higher accuracy in the DOA estimation of low-altitude targets than conventional algorithms under two common impulse noise models, without requiring prior knowledge of impulse noise.

Impact of high altitude on the incidence of postoperative venous thromboembolism and its genetic susceptibility: A meta-analysis and systematic review.

BACKGROUND: The effect of high-altitude (HA) on venous thromboembolism (VTE) and its mechanism remains ambiguous. To clarify this, we aimed to conduct a meta-analysis and systematic review to evaluate the incidence of VTE at HA and comparatively low altitude (LA) and figure out the intrinsic risk factors such as susceptibility genes of patients with VTE at HA. METHODS: We selected studies that explored the risk factors for HA and VTE by searching PubMed, Embase, and Web of Science to analyze the impact of HA on VTE. All relevant studies before August 2021 were screened using the terms ([high altitude] OR [plateau] OR [mountain]) AND ([venous thromboembolism] OR [deep vein thrombosis] OR [pulmonary embolism]). Latest studies on the gene of HA-VTE patients were also summarized and analyzed. RESULTS: Fifteen studies were eventually assessed, and the overall numbers of subjects with and without VTE were 1475 and 286,926 respectively. The overall incidence of VTE, deep vein thrombosis (DVT) and pulmonary embolism (PE) in the HA group was significantly higher than that in the LA group (P < 0.01). The overall incidence of VTE, DVT and PE in the HA group was significantly higher than that in the LA group at 30 days post operation (P < 0.05, P < 0.05 and P < 0.01, respectively). At 90 days post operation, incidence of VTE and PE in the HA group was higher than that in the LA group (P < 0.01and P < 0.01, respectively), but there was no difference in the incidence of DVT (P = 0.07). Regarding endogenous factors, the analysis of genes in patients with HA-VTE revealed numerous targeted genes such as ANG, ACE, lncRNA-LINC00 659/UXT-AS1 and GP4. CONCLUSIONS: We observed a significant association between HA and the overall incidence of VTE and that at 30/90 days post operation, indicating that HA may be a risk factor for VTE.

Newborns physiological differences in low- and high-altitude settings of Ecuador.

Newborns show physiological differences in low- and high-altitude settings of Ecuador; those differences are especially relevant because most important cities in Ecuador are located at high altitude, above 2500 m. This study is an epidemiological, observational, and cross-sectional research performed at San Francisco Hospital in Quito (at 2850 m) and General Hospital in Manta (at 6 m) in the Manabí province. We studied 204 full-term newborns, healthy without any prenatal comorbidities, singleton pregnancy, mestizos, and born of healthy parents born. We found significant differences between the values of red blood cells (RBC), leucocytes, hematocrit, and hemoglobin. There was a difference of 27% more in RBC, 3% at hematocrit, and 0.4 g at hemoglobin in the high-altitude cohort. The leucocyte difference is 1270 cells/µl, which means a difference of 6%. At high-altitude settings, the mean pH was lower than normal values and pO2, pCO2, and HCO3. High-altitude newborns showed RBC of > 4,500,000 cells/µl; leukocytes > 19,000; pO2 ≤ 72 mm Hg; hemoglobin > 17.50 g/dl; and hematocrit > 54%. Both cohorts showed physiological changes of transition to extrauterine life. We observed higher polycythemia, respiratory acidosis, and hypoxemia among high-altitude newborns. High-altitude setting intensifies the physiological changes in hematological and arterial blood gases parameters.

Low-Altitude Windshear Estimation Method Based on Four-Dimensional Frequency Domain Compensation for Fuselage Frustum Conformal Array.

In this paper, a low-altitude wind speed estimation method based on the fuselage frustum conformal array system is proposed. Firstly, based on the signal model of the fuselage conformal array radar, the four-dimensional joint phase compensation of the echo data in the Doppler domain and three-dimensional space-frequency domain is performed by using the four-dimensional frequency domain compensation method. Secondly, the clutter covariance matrix is estimated by the compensated echo data, and a space-time Adaptive Processing (STAP) processor suitable for low-altitude windshear target is constructed to suppress clutter. Finally, the maximum Doppler value of each distance cell is extracted, and the wind velocity is estimated. Simulation results show that the proposed method can effectively suppress clutter and accurately estimate wind speed.

Co-inoculation of yeasts starters: A strategy to improve quality of low altitude Arabica coffee.

The study aimed to improve the quality of dry-processed coffee grown at low altitudes through yeast inoculation, using three species (Saccharomyces cerevisiae CCMA 0543, Torulaspora delbrueckii CCMA 0684, and Candida parapsilosis CCMA 0544) singly and with co-inoculation for fermentation. Important chemical compounds and groups were analyzed by liquid and gas chromatography and Fourier-transform infrared spectroscopy (FTIR). The inoculated coffees with yeast populations around 106 cell/g obtained the highest scores, and the co-inoculation with C. parapsilosis CCMA 0544 and T. delbrueckii CCMA 0684 had the highest score in the sensory analysis (85). Different descriptors were observed in each treatment, and body, flavor, balance, and aftertaste are strongly related to C. parapsilosis CCMA 0544. The fermentation process improved the quality of low-altitude coffees, and the combination of non-Saccharomyces yeasts (C. parapsilosis CCMA 0544 and T. delbrueckii CCMA 0684) was the most indicated as starter cultures.

Heterogeneity in Hematological Parameters of High and Low Altitude Tibetan Populations.

INTRODUCTION: High altitude hypoxia is believed to be experienced at elevations of more than 2500 meters above sea level. Several studies have shed light on the biochemical aspects of high altitude acclimatization, where participants were sojourners to the high altitude from low altitude areas. However, information regarding the difference between the high altitude adapted Tibetans living at high altitude and their counterparts who reside at low altitude are lacking. To understand this, we have measured various hematological parameters in the Tibetan populations, who are residing in both high and low altitudes in India. METHODS: A total of 168 individuals (79 from high altitude (≥4500 meters) and 89 from low altitude (~850 meters) were recruited for this study. Hematological parameters such as red blood cells (RBC) count, hematocrit (HCT), hemoglobin concentration (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were measured from the individuals from high and low altitudes. Serum erythropoietin (EPO) was measured by ELISA. Statistical analyses were performed to compare data from both of the altitudes. Gender-wise comparison of data was reported. Correlation analysis was performed within relevant parameters. RESULTS: Highly significant differences (p <0.0001) between high and low altitude Tibetans were detected in RBC count, HCT, Hb, MCHC in both males and females and in MCV in females. In the case of MCHC, however, age and BMI were potential confounders. Nominally significant differences (p <0.05) were detected in MCV and MCH within males. No significant difference in serum EPO level was found between altitude groups, in any gender. No significant correlation was found between serum EPO with Hb as well as serum EPO with HCT. DISCUSSION: Our study explores significantly lower RBC count, HCT, Hb, MCH, MCHC and higher MCV in long-term Tibetan residents living at low altitude compared to their high altitude counterparts, which is likely due to the outcome of hematological adaptation to a relatively hyperoxic environment in low altitude areas.

Optical and gravito-inertial contributions to the perception and control of height in a simulated Low-Altitude Flight context.

Low-Altitude Flight (LAF) is a flight formation consisting of rapid close ground flight. Perception and control of self-motion, allowing for optimal information collection and rapid adaptation, are of fundamental importance during LAF, but remain largely unexplored. This study aimed to analyse the impact of visuo-vestibular stimuli on the monitoring of height in a motion-based simulated LAF context. Thirteen non-pilots were tested in different environmental conditions, in which optical and gravito-inertial (GI) information were manipulated. The visual environment, displayed with a VR headset, was a low-textured landscape with identical and equally spaced trees throughout the trials. The GI environment was designed thanks to a motion-based simulator. Results showed that participants had better performances in a visuo-vestibular environment than in a visual-only setting, indicating that multi-sensory information was picked-up faster than a mono-sensory structure. Additionally, we found differences in the contribution of vestibular inputs depending on the kind of task. Practitioner summary: Low-Altitude-Flight (LAF) manoeuvres require delicate aircraft control. Two experiments using a large flight simulator investigated how visual and vestibular stimulation contribute to LAF perception and control. Results suggest that both sources of stimulation need to be combined for accurate performance, with consequences for simulator-based training scenarios. Abbreviations: LAF: low altitude flight; GI: gravito-inertial; 1/2/3D: 1/2/3 dimensions; VR: virtual reality; Mvt: movement; GVE: good visual environment; DVE: degraded visual environment; SSQ: simulator motion sickness questionnary; RT: reaction time; DIMSS: dynamic interface modelling and simulation system metric; corrAcf: maximum correlation coefficient; corrLag: maximum correlation lag; DFT: deviation from target; StdJ: standard deviation of the joytick value; NCR: number of control reversal.

Levels of Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), Testosterone and Prolactin at Moderate Altitude Inhabitant Male.

BACKGROUND AND OBJECTIVE: Male fertility depends on the availability of the potent androgen called testosterone. Testosterone production is regulated by the hypothalamic anterior pituitary axis. Two anterior pituitary hormones Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) are involved in spermatogenesis and testosterone production, respectively. Hypoxia, resulting from high altitude, may induce a change in these four hormones and may affect male fertility. This study was done to evaluate and compare the changes that occur in FSH, LH, testosterone and prolactin in males lived in moderate versus low altitude. MATERIALS AND METHODS: This study was conducted on 300 individuals who were categorized based on the altitude of their habitat into two equal groups, namely: Inhabitants at moderate altitudes and inhabitants at low altitudes. A venous blood sample was collected from each individual to measure the levels of FSH, LH and prolactin. RESULTS: Both LH and testosterone levels were significantly lower in high altitude inhabitants compared with low altitude inhabitants (p<0.01). The FSH level showed a significant statistical difference between two groups with a lower level in individuals living at high altitudes compared with low altitude inhabitants but on a value (p<0.05). CONCLUSION: Moderate altitude hypoxia suppresses LH, FSH and testosterone levels as much as high altitude hypoxia does and these changes may depend on prolactin level.

Normoxic low-altitude simulation (at 714 mmHg) improves limb blood perfusion in mice with hindlimb ischemia.

Humans have fewer cardiovascular events and improved outcomes after cardiovascular events when living at low and moderate altitudes (<3000 m) above sea level. We have previously shown that low-altitude simulation using reductions in barometric pressure enhances vasodilation ex vivo in arterial segments and reduces systemic vascular resistance in vivo and can also improve left ventricular function after a myocardial infarction. We hypothesize that low-altitude simulation could also improve hindlimb ischemia, a model of peripheral artery disease in humans. We performed femoral artery ligation to generate hindlimb ischemia in 3-month-old C57BL6 mice. Control group mice (n = 10) recovered at 754 mmHg (control) for 14 days. Treatment group mice (n = 15) were placed in a low-altitude simulation chamber (at 714 mmHg) to recover from surgery for 3-hours daily for 14 days. Hindlimb perfusion imaging using a laser Doppler line scanner was performed for all mice prior to the surgery, and then on days 1, 3, 7, and 14 post-surgery. At 2 weeks, ischemic reserve was significantly higher in the treatment group mice (0.50 ± 0.13 vs. 0.20 ± 0.06; p = 0.01). Treatment mice had higher functional scores and were able to walk better at two weeks. There was approximately three times less HIF1α found via western blotting and a small but statistically significant improvement of lectin perfusion in calf tissue of treatment mice. We conclude that low-altitude simulation improves blood perfusion in murine hindlimb ischemia. This approach may have therapeutic implications for humans with peripheral artery disease.

Managing the drone revolution: A systematic literature review into the current use of airborne drones and future strategic directions for their effective control.

Commercial and private deployment of airborne drones is revolutionising many ecosystems. To identify critical issues and research gaps, our systematic literature review findings suggest that historic issues such as privacy, acceptance and security are increasingly replaced by operational considerations including interaction with and impacts on other airspace users. Recent incidents show that unrestricted drone use can inflict problems on other airspace users like airports and emergency services. Our review of current regulatory approaches shows a need for further policy and management response to both manage rapid and efficient drone usage growth, and facilitate innovation (e.g. intraurban package delivery), with one promising strategic response being low altitude airspace management (LAAM) systems for all drone use cases.

Assessment of Multi-Image Unmanned Aerial Vehicle Based High-Throughput Field Phenotyping of Canopy Temperature.

Canopy temperature (CT) has been related to water-use and yield formation in crops. However, constantly (e.g., sun illumination angle, ambient temperature) as well as rapidly (e.g., clouds) changing environmental conditions make it difficult to compare measurements taken even at short time intervals. This poses a great challenge for high-throughput field phenotyping (HTFP). The aim of this study was to i) set up a workflow for unmanned aerial vehicles (UAV) based HTFP of CT, ii) investigate different data processing procedures to combine information from multiple images into orthomosaics, iii) investigate the repeatability of the resulting CT by means of heritability, and iv) investigate the optimal timing for thermography measurements. Additionally, the approach was v) compared with other methods for HTFP of CT. The study was carried out in a winter wheat field trial with 354 genotypes planted in two replications in a temperate climate, where a UAV captured CT in a time series of 24 flights during 6 weeks of the grain-filling phase. Custom-made thermal ground control points enabled accurate georeferencing of the data. The generated thermal orthomosaics had a high spatial accuracy (mean ground sampling distance of 5.03 cm/pixel) and position accuracy [mean root-mean-square deviation (RMSE) = 4.79 cm] over all time points. An analysis on the impact of the measurement geometry revealed a gradient of apparent CT in parallel to the principle plane of the sun and a hotspot around nadir. Averaging information from all available images (and all measurement geometries) for an area of interest provided the best results by means of heritability. Correcting for spatial in-field heterogeneity as well as slight environmental changes during the measurements were performed with the R package SpATS. CT heritability ranged from 0.36 to 0.74. Highest heritability values were found in the early afternoon. Since senescence was found to influence the results, it is recommended to measure CT in wheat after flowering and before the onset of senescence. Overall, low-altitude and high-resolution remote sensing proved suitable to assess the CT of crop genotypes in a large number of small field plots as is required in crop breeding and variety testing experiments.