ABSTRACT
The change of lung lymph after endotoxin-induced lung injury was studied using a sheep model with chronic lung lymph fistula. After endotoxin injection Ppa、PMV、 Q_L、CL_p、L_A/P_A、and Kf all increased markedly. The ratios of effects of pressure and permeability on Q_L were 1:0.53 and 1:2.31 during phase I and phase H respectively. The results indicated that the formation of pulmonary edema after injury related mainly to the increases of PMV and Kf and to the decrease of effective colloid osmotic pressure. The PMV was the main factor of formating edema during phase I and the permeability was that during phase Ⅱ.
ABSTRACT
A method, which was a modification of that of Stothert et al, was adopted to establish chronic lung lymph fistula in goats from Chongqing area. The procedure consisted mainly that a segment of the thoracic duct was isolated to serve as the efferent of the caudal mediastinal lymph node to collect lung lymph, and an irrigation tube was inserted for the periodic admnistration of heparin solution to prevent the sanguinous lymph from clotting in the early postoperative days. A series of difficulties including high surgical mortality, stoppage of lymph flow, and chyle contamination of the lung lymph were overcome. A trial of the operated goats on steam inhalation injury precipitated instant increase of lung lymph f .ow and an elevation of lymph/plasma ratio of total protein concentration, which suggested that our animal model was suitable and effective in the study of the exchanges of fluid and protein in the lungs. The details of the technique were described and discussed.
ABSTRACT
We observed the pulmonary responses and changes in TXB2 and 6-keto-PGF1a concentrations in plasma and lung lymph in endotoxin induced lung injury. The typical pulmonary responses appeared after the injury. The TXB2 concentrations in plasma and lung lymph increased by 24.5 and 30.7 times and those of 6-keto-PGFla by 10.6 and 15.7 times, respectively, as compared with baseline. The TXB2 and 6-keto-PGF1a concentrations in lymph were 68 and 75 percent higher than those in plasm a respectively. The results indicated that TXA2 and-PGI2 synthesis increased in the lung after injury and that TXA2 and PGI2 were important media in the lung injury.
