Effects of carbon source on the formation, stability, bioactivity and biodiversity of the aerobic granule sludge.

Three aerobic granular sludge systems were operated as sequencing batch reactors (SBR) with acetate, ethanol and glucose as carbon source. The SBR cycle was 6 h, with an anaerobic phase followed by an aerobic phase. The acetate granules (>1.5 mm) had the greatest microbial diversity and better results in terms of removal efficiency for carbon and nutrients (TN ≈ 72% and TP ≈ 42%) and also in the resistance tests. However, partial disintegration was observed. On the other hand, when ethanol was the substrate, the granules were stable, good nitrogen removal was achieved (TN ≈ 53%), but phosphorus removal was not favored (TP ≈ 31%). Glucose presented the lowest efficiency values for nitrogen (TN ≈ 44%) and phosphorous removal (TP ≈ 21%), and the granules formed (<1 mm) had the lowest microbial diversity. Therefore, the carbon source had a high impact on the characteristics of the granules.

Aerobic granular sludge: Cultivation parameters and removal mechanisms.

Aerobic granular sludge (AGS) has been the focus of many investigations, and the main parameters responsible for AGS formation are hydrodynamic shear force, short periods and feast-famine cycles. However, some other parameters are associated with AGS maintenance after long periods of operation. This review evaluates the parameters responsible for AGS formation and maintenance and some reference values are proposed. In addition, some discussions are addressed about the main metabolic pathways that AGS uses for the removal of some compounds, such as nutrients, organic matter, dyes, recalcitrant compounds, among others. Finally, the main microbial groups present in the AGS and their respective functions are discussed. It is also highlighted that many parameters that are taken as reference currently for AGS cultivation and maintenance can be optimized for energy savings, implementation costs, among others, as well as a greater recovery of resources during wastewater treatment, within the scope of the biorefinery concept.