Potential for introducing improved production practices in food legumes with increased food security in Afghanistan

The restricted maximum likelihood method was used to assess performance following the introduction of improved varieties of chickpea and mungbean (an important source of plant protein in Afghanistan) as compared to local varieties using 242 farmer participatory demonstrations laid out in eight districts in Baghlan, Balkh and Uruzgan provinces in Afghanistan from 2009 to 2012. The impact of the varieties introduced on the enhancement of security of food and nutrition of farmers adopting such technologies was also assessed. Taking an average over the study period, chickpea improved varieties (Madad and Sehat) recorded 56 and 72 % more yield over the local ones, respectively, while in case of mungbean varieties, Mai 2008 and Maash 2008 recorded 22 and 30 % more yield over local ones respectively. Though there is a significant yield difference between the improved and the local varieties of both crops, the difference between the improved varieties of chickpea was not significant while it was significant in the case of mungbean. The study revealed a non-zero variance component for variety type [improved vs. local] × year within district interaction for the yield of chickpea while none of the interactions in mungbean had a positive variance component. Risk analysis showed that at a chosen probability level of 90 %, the improved varieties yielded more than local varieties in both crops (> 1.0 t ha1). Thus, the study highlighted the scope for enhancing the security of both food and nutrition in Afghanistan through improved productivity of pulse crops.

Potential for introducing improved production practices in food legumes with increased food security in Afghanistan

The restricted maximum likelihood method was used to assess performance following the introduction of improved varieties of chickpea and mungbean (an important source of plant protein in Afghanistan) as compared to local varieties using 242 farmer participatory demonstrations laid out in eight districts in Baghlan, Balkh and Uruzgan provinces in Afghanistan from 2009 to 2012. The impact of the varieties introduced on the enhancement of security of food and nutrition of farmers adopting such technologies was also assessed. Taking an average over the study period, chickpea improved varieties (Madad and Sehat) recorded 56 and 72 % more yield over the local ones, respectively, while in case of mungbean varieties, Mai 2008 and Maash 2008 recorded 22 and 30 % more yield over local ones respectively. Though there is a significant yield difference between the improved and the local varieties of both crops, the difference between the improved varieties of chickpea was not significant while it was significant in the case of mungbean. The study revealed a non-zero variance component for variety type [improved vs. local] × year within district interaction for the yield of chickpea while none of the interactions in mungbean had a positive variance component. Risk analysis showed that at a chosen probability level of 90 %, the improved varieties yielded more than local varieties in both crops (> 1.0 t ha1). Thus, the study highlighted the scope for enhancing the security of both food and nutrition in Afghanistan through improved productivity of pulse crops.(AU)

Association Study Reveals Novel Genes Related to Yield and Quality of Fruit in Cape Gooseberry (Physalis peruviana L.).

Association mapping has been proposed as an efficient approach to assist plant breeding programs to investigate the genetic basis of agronomic traits. In this study, we evaluated 18 traits related to yield, (FWP, NF, FWI, and FWII), fruit size-shape (FP, FA, MW, WMH, MH, HMW, DI, FSI, FSII, OVO, OBO), and fruit quality (FIR, CF, and SST), in a diverse collection of 100 accessions of Physalis peruviana including wild, landraces, and anther culture derived lines. We identified seven accessions with suitable traits: fruit weight per plant (FWP) > 7,000 g/plant and cracked fruits (CF) < 4%, to be used as parents in cape gooseberry breeding program. In addition, the accessions were also characterized using Genotyping By Sequencing (GBS). We discovered 27,982 and 36,142 informative SNP markers based on the alignment against the two cape gooseberry references transcriptomes. Besides, 30,344 SNPs were identified based on alignment to the tomato reference genome. Genetic structure analysis showed that the population could be divided into two or three sub-groups, corresponding to landraces-anther culture and wild accessions for K = 2 and wild, landraces, and anther culture plants for K = 3. Association analysis was carried out using a Mixed Linear Model (MLM) and 34 SNP markers were significantly associated. These results reveal the basis of the genetic control of important agronomic traits and may facilitate marker-based breeding in P. peruviana.

Genome-wide association study of pre-harvest sprouting resistance in Chinese wheat founder parents

Abstract Pre-harvest sprouting (PHS) is a major abiotic factor affecting grain weight and quality, and is caused by an early break in seed dormancy. Association mapping (AM) is used to detect correlations between phenotypes and genotypes based on linkage disequilibrium (LD) in wheat breeding programs. We evaluated seed dormancy in 80 Chinese wheat founder parents in five environments and performed a genome-wide association study using 6,057 markers, including 93 simple sequence repeat (SSR), 1,472 diversity array technology (DArT), and 4,492 single nucleotide polymorphism (SNP) markers. The general linear model (GLM) and the mixed linear model (MLM) were used in this study, and two significant markers (tPt-7980 and wPt-6457) were identified. Both markers were located on Chromosome 1B, with wPt-6457 having been identified in a previously reported chromosomal position. The significantly associated loci contain essential information for cloning genes related to resistance to PHS and can be used in wheat breeding programs.

Modeling the effects of temperature and pH on the resistance of Alicyclobacillus acidoterrestris in conventional heat-treated fruit beverages through a meta-analysis approach.

In this work, all publicly-accessible published findings on Alicyclobacillus acidoterrestris heat resistance in fruit beverages as affected by temperature and pH were compiled. Then, study characteristics (protocols, fruit and variety, °Brix, pH, temperature, heating medium, culture medium, inactivation method, strains, etc.) were extracted from the primary studies, and some of them incorporated to a meta-analysis mixed-effects linear model based on the basic Bigelow equation describing the heat resistance parameters of this bacterium. The model estimated mean D* values (time needed for one log reduction at a temperature of 95 °C and a pH of 3.5) of Alicyclobacillus in beverages of different fruits, two different concentration types, with and without bacteriocins, and with and without clarification. The zT (temperature change needed to cause one log reduction in D-values) estimated by the meta-analysis model were compared to those ('observed' zT values) reported in the primary studies, and in all cases they were within the confidence intervals of the model. The model was capable of predicting the heat resistance parameters of Alicyclobacillus in fruit beverages beyond the types available in the meta-analytical data. It is expected that the compilation of the thermal resistance of Alicyclobacillus in fruit beverages, carried out in this study, will be of utility to food quality managers in the determination or validation of the lethality of their current heat treatment processes.