ABSTRACT
Chronic systemic inflammation is one of the leading pathogenetic pathways for the development of atherosclerosis in obese patients. In this regard, it seems promising to evaluate the effect of the diet and physical exertion on the proinflammatory activity of monocytes. The purpose of this research was to evaluate the effect of the diet and regular physical trainings on the secretion of monocyte chemotactic factor 1 (MCP-1) by monocytes in obese patients with coronary artery disease. Material and methods. 27 obese participants (body mass index >30 kg/m2) with a confirmed diagnosis of coronary heart disease were recruited. All participants were prescribed with 12 weeks of a specialized diet with a restriction of simple carbohydrates and salt, a 500-kcal daily energy deficit, and with inclusion of cruciferous (200 g per day), seasonal dark berries (70 g per day) and green tea (200 ml per day). The regular assisted physical trainings were also administered. The body composition, blood biochemical parameters and MCP-1 secretion rates in the primary culture of monocytes isolated from blood samples via the immunomagnetic separation method were assessed before and after the intervention. Results. As a result, after the 12-weeks intervention the reliable body weight loss (-4.0%), waist circumference (-4.2%), visceral fat (-5.4%), total cholesterol (-9.8%), LDL-cholesterol (-16.6%) and triglycerides (-26.0%), an improvement in the results of the 6-minute walk test (+10.33%) was achieved, as well as an LPS-stimulated monocytes secretion of MCP-1 decreased by 2.8 times (p=0.005). Conclusion. Overall, the results suggest that diet and regular physical activity in patients with obesity and coronary heart disease may decrease the functional "proinflammatory" activity of monocytes.
Subject(s)
Chemokine CCL2 , Coronary Disease , Monocytes , Obesity , Humans , Monocytes/metabolism , Obesity/diet therapy , Obesity/blood , Obesity/metabolism , Obesity/therapy , Male , Chemokine CCL2/blood , Chemokine CCL2/metabolism , Middle Aged , Female , Coronary Disease/diet therapy , Coronary Disease/metabolism , Coronary Disease/blood , AgedABSTRACT
Objective To investigate the application effect of calcitriol combined with Kuntai capsule in patients with polycystic ovary syndrome. Methods Ninety-seven cases with polycystic ovary syndrome were enrolled in the study and were randomly divided into two groups, 48 cases in the control group were given clomifene citrate capsules, and 49 cases in the observation group were treated with calcitriol combined with Kuntai capsule on the basis of the control group. The treatment effect,endometrial thickness,number of mature follicles,cervical mucus score and sex hormones (luteinizing hormone (LH),androgen,testosterone (T),follicle stimulating hormone (FSH),estradiol (E2)) before and after the treatment,the serum monocyte chemotactic factor -1 (MCP-1) and Leptin level of the two groups were observed and compared. And the rate of pregnancy and the incidence of adverse reactions were recorded. Results The total efficiency of the observation group was 95.92%(47/49),higher than that of the control group (79.17%(38/48))(χ2 ＝6.277,P＝0.012).The endometrial thickness,the number of mature follicles and the cervical mucus score of the observation group were significantly higher than those of the control group(t＝4. 978,7. 175,7. 387,P＜0. 05). After 3 months,the serum levels of T,FSH,LH,MCP-1 and Leptin in the observation group were lower than those in the control group,and the serum E2 level was higher than in the control group(P＜0. 05). The pregnancy rate of the observation group the was 69. 39%( 34/49 ), higher than that of the control group ( 47. 92%( 23/48 )), the difference was statistically significant (χ2＝4. 613,P＝0. 032). The incidence of adverse reactions was 12. 24%(6/49) in the observation group and 8. 33%(4/48) in the control group. There was no significant difference between the two groups(P＞0. 05). Conclusion Calcitriol combined with Kuntai capsule can improve the serum MCP-1,Leptin levels and sex hormone levels in patients with polycystic ovary syndrome,and improve the prognosis of pregnancy rate. Besides,the effect is remarkable with high medication safety.
ABSTRACT
@#In the field of cataract, there is such a widespread phenomenon: the majority of patients have complained thatthe second-eye phacoemulsification is often more painful than the first-eye surgery. Recent studies on this phenomenon have found that after cataract surgery in the first eye, in the second-eye aqueous humor there is a high expression of cytokines which is monocyte chemotactic factor-1.It is the focus of the present study to analyze the mechanism of the increase of pain in the second eye with cataract surgery from the molecular level. Monocyte chemotactic factor-1(monocyte chemoattractant protein, MCP-1)which is considered to be a new member of the family of pain,is an important factor resulting in pain, and its specific receptor CCR2 binding exerts its biological activity. This is a review of the inflammatory cytokine research progress on the increase of pain during the second-eye cataract surgery.
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Objective To study the relationship of serum monocyte chemotactic factor 1 ( MCP-1) and von willebrand factor (vWF) in patients with acute cerebral infarction and the therapeutic effect of butyl phthalide soft capsule.Methods As the research subjects,160 patients of acute cerebral infarction were randomly divided into two groups,80 cases in the observation group,80 cases in the control group.80 cases of healthy physical examination in our hospital were enrolled as the healthy group.The treatment control group was given conventional treatment,treat-ment observation group on the basis of conventional treatment combined with butyl phthalide soft capsule,0.2g orally, three times a day.They were treated for three months.Serum MCP-1 and vWF were detected.Results Before treat-ment,serum MCP-1 and vWF in the observation group and the control group were significantly higher than those in the healthy group(P<0.01).Before treatment,the serum MCP-1 was (480.2 ±34.2)pg/mL,vWF was (2.70 ± 0.45)μg/L,serum MCP-1 and vWF was positively correlated(r=0.286 2,P<0.05).The two groups after treat-ment serum MCP-1 and vWF were significantly decreased,and compared with the control group,the decrease of ser-um vWF and MCP-1 in the observation group was more obvious(P<0.05).Conclusion Serum MCP-1 and vWF has obvious relationship in acute cerebral infarction,butyl phthalide soft capsule can significantly lower the serum con-centration.
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Objective To explore the change of monocyte chemotactic factor-1 protein(MCP-1)and matrix metalloproteinase-9( MMP-9)of patients with coronary artery disease( CAD)following percutaneous coronary interventional( PCI). Methods Fifty patients underwent PCI procedures for CAD compromising a single coronary artery were selected as PCI group and 30 healthy individuals with normal findings by coronary angiography were selected as the control group. Plasma MCP-1 and MMP-9 were measured in all the subjects. Results The plasma MCP-1 level of patients with CAD after PCI was(19. 87 ± 5. 31)ng/ L,higher than that before operation((15. 71 ± 5. 23)ng/ L,t = 3. 95,P < 0. 01). Whereas in the control group,the MCP-1 level after coronary angiography was(13. 78 ± 5. 58)ng/ L,which was as same as that before operation (12. 42 ± 5. 39 ng/ L,P = 0. 34). Plasma MMP-9 level in the CAD patients after PCI procedures was(22. 69 ± 5. 97)mg/ L,higher than that before operation((19. 52 ± 5. 72)mg/ L,t = 2. 71,P < 0. 01). There was no significant difference in term of plasma MMP-9 level in control group befor and after operation((17. 53 ± 5. 51) mg/ L vs.(16. 69 ± 5. 42)mg/ L,P = 0. 55). Conclusion Plasma MCP-1 and MMP-9 increase in CAD patients following PCI procedures. But their roles in the vascular restenosis following the procedures need further investigation.
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Objective To explore the influence of edaravone on serum endothelin(ET),soluble intercellular adhesion moleculel(sICAM-I)and monocyte chemotactic factor 1(MCP-1)and neurological function in acute cerebral infarction patients.Methods 90 patients with acute cerebral infarction were randomly divided into treatment group(additional edaravone)and control group(routine treatment).The serum levels of ET,sICAM-1 and MCP-1were detected by ELESA pretherapy and after treatment.Neurological functional deficit scores(NDS)was utilized for assessment pretherapy and after treatment.Results The levels of serum ET,sICAM-1 and MCP-1 in acute cerebral infarction groups were significantly higher than those of control group(t=4.55,4.62,4.75,4.43,4.61,4.73,all P＜0.05);The levels of serum ET,sICAM-1 and MCP-1,NDS in both two groups after treatment were significantly lower than those before treatment(t=4.12,4.23,4.15,3.91,3.92,3.83,4.52,4.36,all P＜0.05),and the levels of serum ET,sICAM-1,MCP-1 and NDS in the treatment group were significantly lower than those in the control group(t=3.72,3.63,3.53,3.72,all P＜0.05).Conclusion Edaravone can decrease the levels of ET,sICAM-1 and MCP-1in acute cerebral infarction patients.It can lighten acute inflammation and brain injury,protect the tissue of brain.
