The quantity, function and anti-tumor effect of Mucosal associated invariant T cells in patients with bladder cancer.

BACKGROUND: Bladder cancer (BC), a prevalent malignancy in the urinary system, often poses challenges for effective treatment. Immunotherapy, harnessing the immune system, has exhibited promise in early-stage clinical trials. Mucosal associated invariant T (MAIT) cells, a subset of immune cells implicated in various diseases, including certain cancer, have yet to be explored in BC patients. We aimed to investigate the quantity, function, and anti-tumor effects of MAIT cells in BC patients. METHODS: A total of 75 newly diagnosed BC patients and 183 healthy volunteers were included. Blood samples were collected and analyzed to evaluate the quantity and function of MAIT cells. Surgical resection provided BC tissues for further analysis, and the clinical features of BC tumors were collected and their relationship with MAIT cells was explored. RESULTS: MAIT cells were identified in both healthy individuals and BC patients. The proportion of MAIT cells in the peripheral blood of BC patients did not significantly differ from that of healthy controls. However, the study revealed a correlation between the proportion of IFN-γ producing MAIT cells and tumor number and invasion in BC patients. Furthermore, MAIT cells exhibited cytotoxic effects on BC cells in vitro and in vivo. CONCLUSIONS: This study sheds light on the role of MAIT cells in BC. While the quantity of MAIT cells showed no significant change in BC patients, their functional attributes and association with tumor characteristics suggest their potential as an immunotherapy target in BC treatment.

Peripheral blood mucosa-associated constant T cell expression in children with bronchial asthma and its relevance to severity / 中国医师进修杂志

Objective To investigate the peripheral blood mucosa-associated constant T cell (MAIT) expression in children with bronchial asthma and its relevance to severity.Methods Ninetyeight children with bronchial asthma who were treated in Affliated Dongfeng Hospital from May 2016 to May 2019 were selected as the asthma group.Another 60 healthy children who underwent health checkup during the same period were as control group.The levels of MAIT were detected by flow cytometry analyzer using BD FACSAria Ⅱ flow cytometry,and the lung function was detected by Yage lung function tester.The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficacy of peripheral blood MAIT for bronchial asthma.Pearson analysis was used to examine the relationship between MAIT cells and pulmonary function.Results There were no significant differences in the general data of gender,age,and body mass index between the two groups (P ＞ 0.05).The asthma control test (ACT) score,the percentage of first second forced expiratory volume occupied estimated value (FEV1％),the percentage of first second forced expiratory volume occupied forced vital capacity (FEV1/FVC),the percentage of peak expiratory flowoccupied estimated value (PEF),and MAIT cells in asthma group were significantly lower than those in control group (P ＜ 0.05).Serum MAIT identified bronchial asthma and healthy controls with an area under curve (AUC) of 0.900,with a sensitivity of 82.65％,and a specificity of 85.00％.Serum MAIT identified the mild-sustained group and the healthy control group with an AUC of 0.910,with a sensitivity of 94.64％,and a specificity of 81.67％.Correlation analysis showed that MAIT cells in children with bronchial asthma were significantly positively correlated with pulmonary function indexes such as FEV1％,FEV1/FVC and PEF (r =0.159,0.222,0.213,P ＜ 0.05).Conclusions The peripheral blood MA1T in children with bronchial asthma is significantly reduced,which is related to the levels of lung function indicators.MA1T can be used as one of the indicators for the diagnosis and clinical prognosis of patients with bronchial asthma.

Peripheral blood mucosa-associated constant T cell expression in children with bronchial asthma and its relevance to severity / 中国医师进修杂志

Objective@#To investigate the peripheral blood mucosa-associated constant T cell(MAIT) expression in children with bronchial asthma and its relevance to severity.@*Methods@#Ninety-eight children with bronchial asthma who were treated in Affliated Dongfeng Hospital from May 2016 to May 2019 were selected as the asthma group. Another 60 healthy children who underwent health checkup during the same period were as control group. The levels of MAIT were detected by flow cytometry analyzer using BD FACSAria II flow cytometry, and the lung function was detected by Yage lung function tester. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficacy of peripheral blood MAIT for bronchial asthma. Pearson analysis was used to examine the relationship between MAIT cells and pulmonary function.@*Results@#There were no significant differences in the general data of gender, age, and body mass index between the two groups (P>0.05). The asthma control test (ACT) score, the percentage of first second forced expiratory volume occupied estimated value (FEV1%), the percentage of first second forced expiratory volume occupied forced vital capacity (FEV1/FVC), the percentage of peak expiratory flowoccupied estimated value (PEF), and MAIT cells in asthma group were significantly lower than those in control group (P<0.05). Serum MAIT identified bronchial asthma and healthy controls with an area under curve (AUC) of 0.900, with a sensitivity of 82.65%, and a specificity of 85.00%. Serum MAIT identified the mild-sustained group and the healthy control group with an AUC of 0.910, with a sensitivity of 94.64%, and a specificity of 81.67%. Correlation analysis showed that MAIT cells in children with bronchial asthma were significantly positively correlated with pulmonary function indexes such as FEV1%, FEV1/FVC and PEF (r=0.159, 0.222, 0.213, P<0.05).@*Conclusions@#The peripheral blood MAIT in children with bronchial asthma is significantly reduced, which is related to the levels of lung function indicators. MAIT can be used as one of the indicators for the diagnosis and clinical prognosis of patients with bronchial asthma.