A Novel Shunt Zigzag Double-Tap Low-Harmonic Multi-Pulse Rectifier Based on a Three-Stage Power Electronic Phase-Shifting Transformer.

To solve the problem of the large size of traditional industrial frequency phase-shift transformers and the harmonic distortion of multi-pulse wave rectifier systems, this paper proposes a three-stage shunt zigzag power electronic phase-shift transformer based on a double-tap multi-pulse wave rectifier, which combines the power factor correction (PFC) converter with the voltage-type SPWM inverter circuit to form a power electronic converter to realize the frequency boost and power factor correction. Through AC-DC-AC conversion, the frequency of the three-phase AC input voltage is increased, the number of core and coil turns in the transformer is reduced to reduce the size of the phase-shifter transformer, a zigzag structure of the phase-shifter transformer is used to solve the unbalanced distribution of current between the diode bridges, and a passive harmonic suppression method on the DC side is used to generate a loop current by using a group of single-phase rectifier bridges to regulate the input line current of the phase-shifter transformer. The phase-shifted voltage is input into two three-phase diode rectifier bridges to rectify and supply power to the load. Simulation and semi-physical test results show that the proposed method reduces the total harmonic distortion (THD) value of the input current of the phase-shifted transformer to 7.17%, and the THD value of the grid-side input current is further reduced to 2.49%, which meets the harmonic standard and realizes the purpose of power factor correction as well as being more suitable for high-power applications.

Study on Spectrum Shifting and Pulse Splitting of Mode-Locked Fiber Lasers Based on NPR Technology.

We conducted a systematic investigation into the spectral and pulse characteristics of C and L-band Nonlinear Polarization Rotation (NPR) mode-locked fiber lasers effectively employing nonlinear polarization rotation technology. In our experimental setup, we achieved a stable mode-locked state at 1560.076 nm, exhibiting a 3 dB spectral bandwidth of 9.1 nm. As the pump power increased, we observed spectral shifts accompanied by shifts in the first Kelly sideband and the generation of new Kelly sidebands. In this paper, the phenomenon of spectral deviation is elucidated through the interplay of self-phase modulation, group velocity drift, and polarization-dependent isolator (PD-ISO) filter effect, with an analysis of the formation and deviation of Kelly sidebands. Notably, spectral shift persisted even when the pump power exceeded 200 mW. However, continuous pump power escalation led to soliton splitting, resulting in the formation of new soliton beams. Based on the simultaneous generation of spectral shift and pulse splitting, our study contributes to an enhanced understanding of soliton dynamics in ultrafast fiber lasers and lays a foundation for the application of high-repetition-frequency harmonic mode-locked lasers with tunable wavelengths.

Nuclear Transformation of the Marine Pennate Diatom Nitzschia sp. Strain NIES-4635 by Multi-Pulse Electroporation.

Nitzschia is one of the largest genera of diatoms found in a range of aquatic environments, from freshwater to seawater. This genus contains evolutionarily and ecologically unique species, such as those that have lost photosynthetic capacity or those that live symbiotically in dinoflagellates. Several Nitzschia species have been used as indicators of water pollution. Recently, Nitzschia species have attracted considerable attention in the field of biotechnology. In this study, a transformation method for the marine pennate diatom Nitzschia sp. strain NIES-4635, isolated from the coastal Seto Inland Sea, was established. Plasmids containing the promoter/terminator of the fucoxanthin chlorophyll a/c binding protein gene (fcp, or Lhcf) derived from Nitzschia palea were constructed and introduced into cells by multi-pulse electroporation, resulting in 500 µg/mL nourseothricin-resistant transformants with transformation frequencies of up to 365 colonies per 108 cells. In addition, when transformation was performed using a new plasmid containing a promoter derived from a diatom-infecting virus upstream of the green fluorescent protein gene (gfp), 44% of the nourseothricin-resistant clones exhibited GFP fluorescence. The integration of the genes introduced into the genomes of the transformants was confirmed by Southern blotting. The Nitzschia transformation method established in this study will enable the transformation this species, thus allowing the functional analysis of genes from the genus Nitzschia, which are important species for environmental and biotechnological development.

On the Effect of High Stimulation Rates on Temporal Loudness Integration in Cochlear Implant Users.

Long stimuli have lower detection thresholds or are perceived louder than short stimuli with the same intensity, an effect known as temporal loudness integration (TLI). In electric hearing, TLI for pulse trains with a fixed rate but varying number of pulses, i.e. stimulus duration, has mainly been investigated at clinically used stimulation rates. To study the effect of an overall effective stimulation rate at 100% channel crosstalk, we investigated TLI with (a) a clinically used single-channel stimulation rate of 1,500 pps and (b) a high stimulation rate of 18,000 pps, both for an apical and a basal electrode. Thresholds (THR), a line of equal loudness (BAL), and maximum acceptable levels (MALs) were measured in 10 MED-EL cochlear implant users. Stimulus durations varied from a single pulse to 300 ms long pulse trains. At 18,000 pps, the dynamic range (DR) increased by 7.36±3.16 dB for the 300 ms pulse train. Amplitudes at THR, BAL, and MAL decreased monotonically with increasing stimulus duration. The decline was fitted with high accuracy with a power law function (R2=0.94±0.06). Threshold slopes were -1.05±0.36 and -1.66±0.30 dB per doubling of duration for the low and high rate, respectively, and were shallower than for acoustic hearing. The electrode location did not affect the amplitudes or slopes of the TLI curves. THR, BAL, and MAL were always lower for the higher rate and the DR was larger at the higher rate at all measured durations.

Neurophysiologic cut off values for safe resection of patients with supratentorial gliomas.

BACKGROUND: Gliomas have infiltrative nature and tumor volume has direct prognostic value. Optimal resection limits delineated by high-frequency monopolar stimulation with multipulse short train technique is still a matter of debate for safe surgery without (or with acceptable) neurological deficits. It is also an enigma whether the same cut-off values are valid for high and low grades. We aimed to analyze the value of motor mapping/monitoring findings on postoperative motor outcome in diffuse glioma surgery. METHODS: Patients who were operated on due to glioma with intraoperative neuromonitorization at our institution between 2017 and 2021 were analyzed. Demographic information, pre- and post-operative neurological deficit, magnetic resonance images, resection rates, and motor evoked potential (MEP) findings were analyzed. RESULTS: Eighty-seven patients of whom 55 had high-grade tumors were included in the study. Total/near-total resection was achieved in 85%. Subcortical motor threshold (ScMTh) from resection cavity to the corticospinal tract was ≤ 2mA in 17; 3 mA in 14; 4 mA in 6; 5 mA in 7, and ≥5mA in 50 patients. On the 6th month examination, six patients (5 with high-grade tumor) had motor deficits. These patients had changes in MEP that exceeded critical threshold during monitoring. Receiver operating characteristic analysis revealed 2.5 mA ScMTh as the cut-off point for limb paresis after awakening and 6 months for the groups. CONCLUSIONS: Subcortical mapping with MEP monitoring helps to achieve safe wider resection. The optimal safe limit for SCMTh was determined as 2.5 mA. Provided that safe threshold values are maintained in MEP, surgeon may force the functional limits by lowering the SCMTh to 1 mA, especially in low-grade gliomas.

Multi-pulse dynamic patterns in a mode-locked erbium-doped fiber laser based on an Sb2S3-PVA saturable absorber.

Multi-pulse dynamic patterns have been experimentally documented in a passively mode-locked (PML) erbium-doped fiber (EDF) laser using an Sb2S3-PVA saturable absorber (SA). The fundamental mode-locking operation, with a repetition rate of ∼3.22 MHz, a pulse width of ∼2.5 ps, a signal-to-noise ratio (SNR) of ∼50 dB and a peak power over 200 W, was achieved under a pump power from 280 to 360 mW with appropriate polarization states introduced by the polarization controllers (PCs). By rotating the orientation of the intra-cavity PCs carefully and slowly at a pump power of 350 mW, it was found that a multi-pulse bunch was transformed gradually from a single-pulse to a twelve-pulse bunch, with several intermediate transition states of multi-pulse bunches being observed. In addition, other characteristic modes including disordered multi-pulses and soliton rains have been experimentally observed by meticulously adjusting the polarization states of PCs at a pump power of 350 mW. Our systematic study clearly demonstrates that Sb2S3has potential as an effective SA for generating different operation states of multi-pulses in PML anomalous-dispersion EDF lasers.

Micro-Motion Extraction for Marine Targets by Multi-Pulse Delay Conjugate Multiplication and Layered Tracking.

The detection and recognition of marine targets can be improved by utilizing the micro-motion induced by ocean waves. However, distinguishing and tracking overlapping targets is challenging when multiple extended targets overlap in the range dimension of the radar echo. In this paper, we propose a multi-pulse delay conjugate multiplication and layered tracking (MDCM-LT) algorithm for micro-motion trajectory tracking. The MDCM method is first applied to obtain the conjugate phase from the radar echo, which enables high-precision micro-motion extraction and overlapping state identification of extended targets. Then, the LT algorithm is proposed to track the sparse scattering points belonging to different extended targets. In our simulation, the root mean square errors of the distance and velocity trajectories were better than 0.277 m and 0.016 m/s, respectively. Our results demonstrate that the proposed method has the potential to improve the precision and reliability of marine target detection through radar.

Two phases based training method for designing codewords for a set of perceptrons with each perceptron having multi-pulse type activation function.

This paper proposes a two phases-based training method to design the codewords to map the cluster indices of the input feature vectors to the outputs of the new perceptrons with the multi-pulse type activation functions. Our proposed method is applied to classify two types of the tachycardias. First, the total number of the new perceptrons is initialized as the dimensions of the input feature vectors. Next, a set of new perceptrons with each new perceptron having a single pulse type activation function is designed. Then, the new perceptrons with the multi-pulse type activation function are designed based on those new perceptrons with the single pulse type activation function. After that, the codewords are assigned according to the outputs of the new perceptrons with the multi-pulse type activation functions. Finally, a condition on the codewords is checked. The significance of this work is to guarantee to achieve the no classification error efficiently through using more than one new perceptron with the multi-pulse type activation if the feature space can be linearly partitioned into the multiple clusters. The computer numerical simulation results show that our proposed method outperforms the conventional perceptrons with the sign type activation function.

Investigating the input power quality of multi-pulse AC-DC power converter fed induction motor drives.

Optimization of supply current total harmonic distortion (THD) of multi-pulse AC-DC power converter fed induction motor drive (IMD) is always a challenging issue. Higher amount of supply current THD degrades the input power quality of IMD. The supply current THD should be controlled in such a way so that it adheres the power quality standard of IEEE-519. With the increase of the pulse number of multi-pulse AC-DC converter, supply current THD increases. In this work, an investigation has been carried out on 6-pulse, 12-pulse, 18-pulse, and 24-pulse AC-DC power converters based IMD. A thorough analysis of input current profile, THD, dynamic responses including stator current, speed, torque profile of the induction motor are highlighted in this work with the various load perturbation conditions. This work will provide a message to the industrial community about the proper selection of AC-DC power converter for IMD application considering power quality and circuit configuration issues. All the investigating works are conducted in MATLAB/Simulink platform.

A New Insight into High-Aspect-Ratio Channel Drilling in Translucent Dielectrics with a KrF Laser for Waveguide Applications.

A new insight into capillary channel formation with a high aspect ratio in the translucent matter by nanosecond UV laser pulses is discussed based on our experiments on KrF laser multi-pulse drilling of polymethyl methacrylate and K8 silica glass. The proposed mechanism includes self-consistent laser beam filamentation along a small UV light penetration depth caused by a local refraction index increase due to material densification by both UV and ablation pressure, followed by filamentation-assisted ablation. A similar mechanism was shown to be realized in highly transparent media, i.e., KU-1 glass with a multiphoton absorption switched on instead of linear absorption. Waveguide laser beam propagation in long capillary channels was considered for direct electron acceleration by high-power laser pulses and nonlinear compression of excimer laser pulses into the picosecond range.

eABR THR Estimation Using High-Rate Multi-Pulse Stimulation in Cochlear Implant Users.

We estimated the electrically-evoked auditory brainstem response thresholds (eABR THRs) in response to multi-pulses with high burst rate of 10,000 pulses-per-second (pps). Growth functions of wave eV amplitudes, root mean square (RMS) values, peak of phase-locking value (PLV), and the lowest valid data point (LVDP) were calculated in 1-, 2-, 4-, 8-, and 16-pulses conditions. The growth functions were then fitted and extrapolated with linear and exponential functions to find eABR THRs. The estimated THRs were compared to psychophysical THRs determined for multi-pulse conditions as well as to the clinical THRs measured behaviorally at the rate of 1,000 pps. The growth functions of features showed shallower growth slopes when the number of pulses increased. eABR THRs estimated in 4-, 8-, and 16-pulses conditions were closer to the clinical THRs, when compared to 1- and 2-pulses conditions. However, the smallest difference between estimated eABR THRs and clinical THRs was not always achieved from the same number of pulses. The smallest absolute difference of 30.3 µA was found for the linear fittings on growth functions of eABR RMS values in 4-pulses condition. Pearson's correlation coefficients (PCCs) between eABR THRs and psychophysical THRs were significant and relatively large in all but 16-pulses conditions. The PCCs between eABR THRs and clinical THRs, however, were smaller and in less cases significant. Results of this study showed that eABRs to multi-pulse stimulation could, to some extent, represent clinical stimulation paradigms, and thus in comparison to single pulses, could estimate clinical THRs with smaller errors.

Intelligent Recognition of Chirp Radar Deceptive Jamming Based on Multi-Pulse Information Fusion.

The perception of jamming types is very important for protecting our radar in complex electromagnetic environments. Radar active deceptive jamming based on digital radio frequency memory (DRFM) has a high coherence with the target echo, which confuses the information of the target echo and achieves the effect of hiding the real target. Traditional deceptive jamming recognition methods need to extract complex features and artificially set classification thresholds, which is inefficient. The existing neural network-based jamming identification methods still follow the pattern of signal modulation-type identification, so there are fewer types of jamming that can be identified, and the identification accuracy is low in the case of low jamming-to-noise ratios (JNR). This paper studies the input of jamming recognition networks and proposes an improved intelligent identification method for chirp radar deceptive jamming. This method fuses three short-time Fourier transform time-frequency graphs disturbed by three consecutive pulse periods into a new graph as the input of the convolutional neural network (CNN). Using a CNN to classify the time-frequency image has realized the recognition of a variety of common deceptive jamming techniques. Similarly, by changing the network input, the original signal is used to replace the echo signal, which improves the accuracy of the jamming recognition in the case of a low JNR.

High frequency ultrasound nonlinear scattering from porphyrin nanobubbles.

Emerging contrast imaging studies have highlighted the potential of nanobubbles for both intravascular and extravascular applications. Reports to date on nanobubbles have generally utilized low frequencies (<12 MHz), high concentrations (>109 mL-1), and B-mode or contrast-mode on preclinical and clinical systems. However, none of these studies directly examined nanobubble acoustic signatures systematically to implement nonlinear imaging schemes in a methodical manner based on nanobubble behaviour. Here, nanobubble nonlinear behaviour is investigated at high frequencies (12.5, 25, 30 MHz) and low concentration (106 mL-1) in a channel phantom, with different pulse types in single- and multi-pulse sequences to examine behaviour under conditions relevant to high frequency imaging. Porphyrin nanobubbles are demonstrated to initiate nonlinear scattering at high frequencies in a pressure-threshold dependent manner, as previously observed at low frequencies. This threshold behaviour was then utilized to demonstrate enhanced nanobubble imaging with pulse inversion, amplitude modulation, and a combination of the two, progressing towards the improved sensitivity and expanded utility of these ultrasound contrast agents.

Evaluating and Comparing Behavioural and Electrophysiological Estimates of Neural Health in Cochlear Implant Users.

Variations in neural health along the cochlea can degrade the spectral and temporal representation of sounds conveyed by cochlear implants (CIs). We evaluated and compared one electrophysiological measure and two behavioural measures that have been proposed as estimates of neural health patterns, in order to explore the extent to which the different measures provide converging and consistent neural health estimates. All measures were obtained from the same 11 users of the Cochlear Corporation CI. The two behavioural measures were multipulse integration (MPI) and the polarity effect (PE), both measured on each of seven electrodes per subject. MPI was measured as the difference between thresholds at 80 pps and 1000 pps, and PE as the difference in thresholds between cathodic- and anodic-centred quadraphasic (QP) 80-pps pulse trains. It has been proposed that good neural health corresponds to a large MPI and to a large negative PE (lower thresholds for cathodic than anodic pulses). The electrophysiological measure was the effect of interphase gap (IPG) on the offset of the ECAP amplitude growth function (AGF), which has been correlated with spiral ganglion neuron density in guinea pigs. This 'IPG offset' was obtained on the same subset of electrodes used for the behavioural measures. Despite high test-retest reliability, there were no significant correlations between the neural health estimates for either within-subject comparisons across the electrode array, or between-subject comparisons of the means. A phenomenological model of a population of spiral ganglion neurons was then used to investigate physiological mechanisms that might underlie the different neural health estimates. The combined experimental and modelling results provide evidence that PE, MPI and IPG offset may reflect different characteristics of the electrode-neural interface.

Investigation of Electrically Evoked Auditory Brainstem Responses to Multi-Pulse Stimulation of High Frequency in Cochlear Implant Users.

We investigated the effects of electric multi-pulse stimulation on electrically evoked auditory brainstem responses (eABRs). Multi-pulses with a high burst rate of 10,000 pps were assembled from pulses of 45-µs phase duration. Conditions of 1, 2, 4, 8, and 16 pulses were investigated. Psychophysical thresholds (THRs) and most comfortable levels (MCLs) in multi-pulse conditions were measured. Psychophysical temporal integration functions (slopes of THRs/MCLs as a function of number of pulses) were -1.30 and -0.93 dB/doubling of the number of pulses, which correspond to the doubling of pulse duration. A total of 15 eABR conditions with different numbers of pulses and amplitudes were measured. The morphology of eABRs to multi-pulse stimuli did not differ from those to conventional single pulses. eABR wave eV amplitudes and latencies were analyzed extensively. At a fixed stimulation amplitude, an increasing number of pulses caused increasing wave eV amplitudes up to a certain, subject-dependent number of pulses. Then, amplitudes either saturated or even decreased. This contradicted the conventional amplitude growth functions and also contradicted psychophysical results. We showed that destructive interference could be a possible reason for such a finding, where peaks and troughs of responses to the first pulses were suppressed by those of successive pulses in the train. This study provides data on psychophysical THRs and MCLs and corresponding eABR responses for stimulation with single-pulse and multi-pulse stimuli with increasing duration. Therefore, it provides insights how pulse trains integrate at the level of the brainstem.

Multiple pulse electroporation of lactic acid bacteria Lactococcus lactis and Lactobacillus casei.

Genetic manipulation of lactic acid bacteria is often difficult due to the inability to transform them with high efficiency. Multi-pulse electroporation offers a simple approach to increase transformation efficiencies. Using cells grown with 1% glycine and pretreated with lithium acetate and dithiothreitol, multi-pulse electroporation (five pulses of 12.5 kV cm-1) of Lactococcus lactis JB704 cells resulted in a transformation efficiency of up to 1.2 × 106 colony forming units (CFU) µg-1 pGK13, an 8-fold increase in the transformation efficiency compared to single pulse electroporation. Other cell growth and pretreatment conditions with JB704 resulted in lower transformation efficiencies but had 4-fold to 27-fold higher transformation efficiencies with the five pulse electroporations. With similarly grown and pretreated Lactobacillus casei 32G cells, multi-pulse electroporation (five pulses of 7.5 kV cm-1) resulted in a mean transformation efficiency of 7.3 × 103 CFU µg-1 pTRKH2, a 4-fold increase in the transformation efficiency compared to single pulse electroporation.

Rationalization of solid-state NMR multi-pulse decoupling strategies: Coupling of spin I = ½ and half-integer quadrupolar nuclei.

In this paper we undertake a study of the decoupling efficiency of the Multiple-Pulse (MP) scheme, and a rationalization of its parameterization and of the choice of instrumental set up. This decoupling scheme is known to remove the broadening of spin-1/2 spectra I, produced by the heteronuclear scalar interaction with a half-integer quadrupolar nucleus S, without reintroducing heteronuclear dipolar interaction. The resulting resolution enhancement depends on the set-up of the length of the series of pulses and delays of the MP, and some intrinsic material and instrumental parameters. Firstly through a numerical approach, this study investigates the influence of the main intrinsic material parameters (heteronuclear dipolar and J coupling, quadrupolar interaction, spin nature) and instrumental parameters (spinning rate, pulse field strength) on efficiency and resolution enhancement of the scalar decoupling scheme. A guideline is then proposed to obtain quickly and easily the best resolution enhancement via the rationalization of the instrumental and parameter set up. It is then illustrated and tested through experimental data, probing the efficiency of MP-decoupling set up using this guideline. Various spin systems were tested (31P-51V in VOPO4, 31P-93Nb in NbOPO4, 119Sn-17O in Y2Sn2O7), combined with simulations results.

Experimental and numerical investigation on the transient vascular thermal response to multi-pulse Nd:YAG laser.

BACKGROUND AND OBJECTIVE: Port wine stains (PWS) are congenital vascular malformations that progressively darken and thicken with age. Laser therapy is currently the most effective way in clinical practice for PWS. A 1,064 nm Nd:YAG laser in the near-infrared band can achieve a deeper treatment depth compared to the current widely adopted pulsed dye laser. However, because of its relatively weak absorption by blood, single-pulse Nd:YAG laser requires high energy density to cause effective vessel damage, but may inflict undesirable burning to surrounding collagen. Multi-pulse laser has great potential in clinical treatment because it needs less energy density for each pulse. This paper presented an experimental and theoretical study of the transient thermal effects of low-energy multi-pulse Nd:YAG laser on blood vessels. STUDY DESIGN/MATERIALS AND METHODS: In vivo experiments were performed on dorsal skin chamber. By using a high speed camera (up to 2,000 fps), the complete and dynamic thermal response of blood vessels during laser irradiation and between pulse intervals was obtained. In vitro experiment in capillary tubes and Numerical simulations by two-scale heat transfer model were also conducted to further explore the in vivo experimental findings. RESULTS: The complete and dynamic response of blood vessels were obtained, including vessel dilation, thrombus formation, partial vessel constriction, thread-like constriction, cavitation and bubbles, and hemorrhage. Thread-like constriction is the desirable treatment end point, which will only occur after thrombus completely occludes the vessel lumen. Cavitation can cause hemorrhage when thrombus fails to occlude the vessel lumen. In vitro experiment found that vessel constriction was due to the constriction of thrombus induced by laser irradiation. Theoretical investigation revealed that the mechanism for the effective reduction of energy density by multi-pulse Nd:YAG laser was due to enhanced light absorption of the blood with thrombus formation. CONCLUSIONS: For multi-pulse treatment, laser parameters are recommended as repetition rate of 10 Hz and pulse number of 10. The incident energy in each pulse should be strong enough to induce blood coagulation through seven or eight pulses and should be lower than the threshold of blood cavitation. Lasers Surg. Med. 49:852-865, 2017. © 2017 Wiley Periodicals, Inc.

Efficient transformation of Staphylococcus aureus using multi-pulse electroporation.

A new multi-pulse electroporation system was evaluated to transform Staphylococcus aureus. Compared to the conventional electroporation system, it yielded high transformation efficiency to obtain more than 3.9×105S. aureus RN4220 transformed cells/1µg plasmid DNA using a single electroporation by manipulating the poring pulse and transfer pulse.

Multi-Pulse Excitation for Underwater Analysis of Copper-Based Alloys Using a Novel Remote Laser-Induced Breakdown Spectroscopy (LIBS) System.

In this work, the use of multi-pulse excitation has been evaluated as an effective solution to mitigate the preferential ablation of the most volatile elements, namely Sn, Pb, and Zn, observed during laser-induced breakdown spectroscopy (LIBS) analysis of copper-based alloys. The novel remote LIBS prototype used in this experiments featured both single-pulse (SP-LIBS) and multi-pulse excitation (MP-LIBS). The remote instrument is capable of performing chemical analysis of submersed materials up to a depth of 50 m. Laser-induced breakdown spectroscopy analysis was performed at air pressure settings simulating the conditions during a real subsea analysis. A set of five certified bronze standards with variable concentration of Cu, As, Sn, Pb, and Zn were used. In SP-LIBS, signal emission is strongly sensitive to ambient pressure. In this case, fractionation effect was observed. Multi-pulse excitation circumvents the effect of pressure over the quantitative analysis, thus avoiding the fractionation phenomena observed in single pulse LIBS. The use of copper as internal standard minimizes matrix effects and discrepancies due to variation in ablated mass.