ABSTRACT
Notopterygium incisum Ting ex H. T. Chang is a rare and endangered traditional Chinese medicinal plant. In this research, we built a comprehensive habitat suitability (CHS) model to analyze the potential suitable habitat distribution of this species in the present and future in China. First, using nine different algorithms, we built an ensemble model to explore the possible impacts of climate change on the habitat distribution of this species. Then, based on this model, we built a CHS model to further identify the distribution characteristics of N. incisum-suitable habitats in three time periods (current, 2050s, and 2070s) while considering the effects of soil and vegetation conditions. The results indicated that the current suitable habitat for N. incisum covers approximately 83.76 × 103 km2, and these locations were concentrated in the Tibet Autonomous Region, Gansu Province, Qinghai Province, and Sichuan Province. In the future, the areas of suitable habitat for N. incisum would significantly decrease and would be 69.53 × 103 km2 and 60.21 × 103 km2 in the 2050s and 2070s, respectively. However, the area of marginally suitable habitat would remain relatively stable. This study provides a more reliable and comprehensive method for modelling the current and future distributions of N. incisum, and it provides valuable insights for highlighting priority areas for medicinal plant conservation and resource utilization.
ABSTRACT
Objective: To study coumarins from Notopterygium incisum and their anti-inflammatory effect. Methods: Coumarins were separated and purified by repeated column chromatography on silica gel and HPLC, and their chemical structures were determined by spectral data analyses of MS and NMR. Lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells accompanying overproduction of pro-inflammatory mediator nitric oxide (NO) were applied to investigate the anti-inflammatory effect of these purified coumarins. Results: Twenty-four coumarins were obtained and identified as isoimperatorin (1), angenomalin (2), psoralen (3), bergapten (4), capillarin (5), osthenol (6), 5-dehydronotopterol (7), anhydronotopoloxide (8), 7'-O-methylnotoptol (9), bergamottin (10), 7-isopentenyloxy-6-methoxy-coumarin (11), pabulenol (12), notopterol (13), demethylfuropinarine (14), notoptol (15), cnidilin (16), 6-isopentenyloxyumbelliferone (17), nodakenitin (18), isopimpinellin (19), nodakenin (20), decuroside V (21), decuroside I (22), marmesin-11-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (23), and forbesoside (24), respectively. Coumarins 7-10, 13, and 15 exhibited potent inhibitory activity against LPS-induced NO production in RAW 264.7 cells with half maximal inhibitory concentration values from 8.50 to 35.12 μmol/L. Conclusion: Compound 7 is a new natural product; compound 17 is obtained from the roots and rhizomes of N. incisum for the first time; Unsaturated double bond on C-5 in the aromatic ring significantly improved the anti-inflammatory activity of coumarins.
ABSTRACT
Objective: To investigate the coumarin compounds from Notopterygium incisum and their anti-oxidant activities. Methods The coumarin compounds and their analogues were separated and purified by recrystallization, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative HPLC. The structure was identified by modern spectroscopy. The isolated compound was tested for anti-oxidant activity by DPPH and ABTS assay. Results Ten compounds were isolated from the dichloromethane extraction layer of 70% ethanol extract, which were identified as bergaptol (1), d-laserpitin (2), falcarindiol (3), phenethyl ferulate (4), selinidin (5), archangelicin (6), notoptol (7), nodakenetin (8), (+)-cis-khellactone (9), and oxypeucedanin hydrate (10). The anti-oxidant activity of these compounds was tested by DPPH and ABTS methods. Conclusion Compounds 2 and 6 are isolated from this plant for the first time. Compounds 1 and 7 have shown the strongest anti-oxidant activity. The structure-activity relationship (SAR) studies revealed the -OH and unsaturated double bond on C-5′ in the aromatic ring significantly improved the anti-oxidant activity than other coumarin compounds.
ABSTRACT
Objective: To investigate the chemical constituents of Notopterygium incisum. Methods: The chemical constituents were isolated by silica gel column chromatography, HPLC, and Sephadex LH-20 column chromatography. Their structures were identified on the basis of spectral data analysis. Results: Fourteen compounds were isolated and identified as 7-hydroxycoumarin (1), 5,7-dimethoxycoumarin (2), nodakenetin (3), bergaptol (4), bergapten (5), isopimpinellin (6), byakangelicin (7), (+)-cis-khellactone (8), (-)-trans-khellactone (9), p-hydroxypenehtyl anisate (10), 5-methoxy-8-hydroxypsoralen (11), ferulic acid (12), isoimperatorin (13), and isopsoralen (14). Conclusion: Compounds 2, 7-9, and 11 are isolated from the plants of Notopterygium H. Boiss. for the first time.
ABSTRACT
Objective: To analyze the genetic diversity and genetic structure of 245 plant samples in Notopterygium incisum from different regions. Methods: Genetic diversity and genetic structure of 245 samples of N. incisum were studied using chloroplast cpDNA trnT-trnL direct sequencing method and Neighbor-joining (NJ) method. Results: Haplotype diversity (Hd) and nucleotide diversity (Pi) at the species level were 0.873 and 0.00407, respectively. At the population level, Hd ranged with 0.000-0.900, and Pi ranged with 0.000-0.0544. It indicated that N. incisum had a moderate level of genetic diversity than N. franchetii plants of the genus Notopterygium Boiss. Molecular variance analysis (AMOVA) indicated that high genetic differentiation (77.55%) existed among populations, and gene flow was low (Nm=0.145) among populations. Based on the NJ tree, the 31 wild populations tested were clustered into two groups. Conclusion: N. incisum has the midium cpDNA diversity and higher population genetic differentiation.
