ABSTRACT
OBJECTIVE: Infection with human tumor viruses is one of the hypothesized causes of cancer. The current investigation aimed to explore the presence and quantitative analysis of a new human tumor virus, Merkel cell polyomavirus (MCPyV) in tissue samples of 114 patients with oral cavity lesions including oral squamous cell carcinoma (OSCC), oral lichen planus (OLP), Dysplasia and oral irritation fibroma (OIF) in Northern Iran. METHODS: From 114 formalin fixed paraffin embedded samples; 35 with SCC, 29 with OLP, 14 with dysplasia and 36 with OIF were cut, deparaffinized and DNA was extracted. Quantitative detection of MCPyV large T antigen was performed by absolute quantitative Real-Time PCR. RESULT: MCPyV DNA was detected in 30.6% (n: 11/36) of IF, 24.1% (n; 7/29) of OLP, 21.4% (n:3/14) of dysplasia and 20% (n;7/35) of OSCC samples. The mean MCPyV DNA copy number was 2.32×10-2 ± 3.97 ×10-2, 2.02×10-2 (SD=3.13×10-2), 2.69×10-4 (SD=2.51×10-4), and 2.56×10-4 (SD=6.73×10-4) per cell in OSCC, dysplasia and both of OLP and OIF samples, respectively (P=0.76). CONCLUSION: This study provides the first data from Iran regarding the presence of MCPyV genome in oral cavity lesions and oral cancer. These results also emphasize that MCPyV has an active role in the occurrence of oral lesions and progression to cancer. Further studies should be carried out to clarify the role of MCPyV in oral cavity lesions.
Subject(s)
Merkel cell polyomavirus/isolation & purification , Mouth Diseases/epidemiology , Mouth Neoplasms/epidemiology , Polyomavirus Infections/epidemiology , Tumor Virus Infections/epidemiology , Adolescent , Adult , Aged , Aged, 80 and over , Biopsy , Carcinoma, Merkel Cell/epidemiology , Carcinoma, Merkel Cell/virology , Child , DNA, Viral/analysis , Female , Fibroma/epidemiology , Fibroma/virology , Head and Neck Neoplasms/epidemiology , Head and Neck Neoplasms/virology , Humans , Iran/epidemiology , Lichen Planus, Oral/epidemiology , Lichen Planus, Oral/virology , Male , Merkel cell polyomavirus/genetics , Middle Aged , Mouth/virology , Mouth Diseases/virology , Mouth Neoplasms/virology , Polyomavirus Infections/virology , Prevalence , Real-Time Polymerase Chain Reaction , Skin Neoplasms/epidemiology , Skin Neoplasms/virology , Squamous Cell Carcinoma of Head and Neck/epidemiology , Squamous Cell Carcinoma of Head and Neck/virology , Tumor Virus Infections/virology , Young AdultABSTRACT
BACKGROUND: Epstein-Barr Virus (EBV) is a human oncogenic virus that can lead to cancer in lymphoid and epithelial cells and is one of the hypothesized causes of oral cavity lesions including oral squamous cell carcinoma (OSCC), but the etiological association remains undetermined. The present investigation aimed to explore the EBV presence, viral load, and EBV-encoded small RNA (EBER) sequence variation in tissue samples of patients with OSCC and other oral cavity lesions including oral lichen planus (OLP), and oral irritation fibroma (OIF). METHODS: In total, 88 oral cavity samples (23 with OSCC, 29 with OLP, and 36 with OIF diagnosis) were examined by Real-Time PCR technique and some of them were sequenced. RESULTS: Viral EBER sequence was detected in 6 out of the 23 OSCC (31.4%), 6 out of the 29 OLP (20.7%), and 3 out of the 36 OIF cases (8.3%). The mean EBV copy number was higher in OSCC samples (1.2 × 10-2 ± 1.3 × 10-2 copies/cell) compared to OLP (2.2 × 10-3 ± 2.6 × 10-3 copies/cell) and OIF (2.4 × 10-4 ± 2.0 × 10-4 copies/cell) samples, although this difference was not statistically significant (P = 0.318). The EBER gene was amplified and sequenced in 5 OSCC, 3 OLP, and 2 OIF samples with high EBV viral load. One OSCC, two OLP, and two OIF isolates showed different nucleotide variations compared with EBV-WT and AG876 prototype sequences: C6834T, C6870T, C6981T, C7085T, C7085G, and C7094T. CONCLUSION: In our study the presence of more than one genome copies per tumor cell indicates the possible role of EBV infection in oral cancers. However, more studies should be conducted to clarify the role of EBV in OSCC carcinogenesis.
Subject(s)
Carcinoma, Squamous Cell , Epstein-Barr Virus Infections , Head and Neck Neoplasms , Lichen Planus, Oral , Mouth Neoplasms , Carcinoma, Squamous Cell/genetics , Herpesvirus 4, Human/genetics , Humans , Mouth Neoplasms/genetics , RNA , Squamous Cell Carcinoma of Head and NeckABSTRACT
Irritation fibroma are the most common form of reactive lesions found within the oralcavity. These lesions normally attain a small size and show a slow growth rate. This paper reports a case ofgigantic intraoral irritational fibroma with a history of rapid growth. Complete excision was performed andthe specimen was sent for immunohistochemistry (IHC) staining. It was found positive for vimentin, hencereported as irritation fibroma.
Los fibromas irritativos son las forma más común de lesiones reactivas encontradas en la cavidad oral. Estas lesiones normalmente son de pequeño tamaño y muestran una tasa de crecimiento lenta. Este artículo reporta el caso de un fibroma irritativo gigante intraoral con historia de crecimiento rápido. Se realizó la extirpación completa y fue enviado para análisis inmunohistoquímico. Arrojó positivo para Vimentina, por lo tanto, se reportó como fibroma irritativo.
