[Study of gene expression of transcription factors T cells during aging].

The level of expression of transcription factor genes (GATA-3, TBX21, IL23A), and changing of oxidative modification of proteins in young and elderly healthy persons was studied. The results of evaluation of gene expression, GATA-3 in lymphocytes showed the increased expression of GATA-3 in elderly people in comparison with the young. Women demonstrated higher expression of GATA-3 in compare with men. Study of IL-2p showed reduced levels of expression in aged humans compared to young. TVH21 expression level showed a reduction expression in both men and women. Comparative analysis of protein oxidation in blood plasma of young and elderly people showed an increase in the intensity of oxidative modification of proteins in the elderly.

Increased Prooxidative Activity Stimulating the Oxidative Modification of Proteins in the Umbilical Venous Plasma and Placenta of Preeclampsia / 대한주산의학회잡지

OBJECTIVE: This study was performed to compare the prooxidative activity stimulating the protein carbonyl formation by cephalosporins in the umbilical venous and placenta of preeclampsia with that of normal pregnancy. METHODS: Lipid peroxide levels in the umbilical venous plasma and placental tissue homogenates of normal pregnancy (n=12) and preeclampsia (n=12) were measured by thiobarbituric acid reaction. The basal protein carbonyl contents in the umbilical venous plasma and placental tissue homogenates of normal pregnancy (n=12) and preeclampsia (n=12) were determined by the 2,4-dinitrophenylhydrazine (DNPH) method. After samples of them were mixed and incubated up to 5 hours with 0.2 mL of 1 mM moxalactam or cephalothin, the protein carbonyl contents in them were measured by DNPH. RESULTS: Protein carbonyls formation by moxalactam and cephalothin in the umbilical venous plasma and of women with preeclampsia were significantly higher than that of women with normal pregnancy (8.5+/-2.0 vs. 6.6+/-1.4 nmol/mg protein, p<0.05, 7.6+/-1.6 vs. 6.2+/-1.2 nmol/mg protein, p<0.05). Protein carbonyls formation by moxalactam and cephalothin in the placental tissue homogenates of women with preeclampsia were significantly higher than that of women with normal pregnancy (17.6+/-5.3 vs. 13.0+/-4.2 nmol/mg protein, p<0.05, 16.1+/-5.2 vs. 12.5+/-4.4 nmol/mg protein, p<0.05). There were significant positive correlations between lipid peroxide and cephalosporins induced protein carbonyls levels of umbilical venous plasma, and placental tissue homogenates (p<0.01). CONCLUSION: These results suggest that increase in the prooxidative activity stimulating the oxidative modification of proteins in placenta may be involved in the pathogenesis of preecalmpsia.