Preservation of cv. Jubileu peaches in a conventional cold storage system under a dynamic controlled atmosphere / Conservação de pêssegos cv. Jubileu em sistema refrigerado convencional, atmosfera controlada e dinâmica

The peach tree (Prunus persica) has a considerable global economic impact because its fruits are consumed worldwide. As climacteric fruits, peaches ripen after harvest and are also highly perishable postharvest. The aim of this study was to investigate alternatives for extending storage time and preventing a decline in quality in peaches using conventional cold storage (CS) compared with controlled atmosphere (CA) and ultralow oxygen (ULO) systems. A completely randomized design was used, with a 3 x 6 factorial scheme (3 storage systems x 6 storage times), thirteen parameters assessed by analysis of variances and significance by Tukey's test and regression. Total soluble solids (TSS) varied from 12.72 to 16.07ºBrix, titratable acidity (TA) declined during storage and pH varied significantly among the systems used from 40 days of storage onwards. The best TSS/TA ratio was obtained under ULO after 40 days, while firmness and weight loss declined in CA and ULO, contrasting with the brightly colored pulp. Reducing and non-reducing sugars decreased under CA and ULO at 30 and 40 days of storage, whereas phenolic compounds and antioxidant activity remained similar in these systems at 30, 40 and 50 days. Carotenoids remained stable for up to 40 days in ULO.CA and ULO maintained the best physical and chemical quality parameters, both systems being the most recommended. The phytochemical compounds analyzed in this study changed little during storage in the systems analyzed.(AU)

O pessegueiro (Prunus persica) apresenta grande impacto econômico global, por produzirem frutos apreciados em todo o mundo. Seus frutos climatérios amadurecem após a colheita, além disso, possuem alta perecibilidade pós-colheita. O objetivo deste trabalho foi buscar alternativas que ampliem ainda mais o tempo de armazenamento evitando-se o decaimento da qualidade dos pêssegos, utilizando o sistema refrigerado convencional (SAR) em contraponto com a atmosfera controlada (SAC) e dinâmica com ultrabaixo oxigênio (SAUO). O delineamento experimental foi inteiramente casualizado em um esquema fatorial 3 x 6 (3 sistemas x 6 períodos) de armazenamento, e treze parâmetros analisados pelo teste análise de variância, significância pelo teste Tukey e regressão. Os valores de sólidos solúveis variaram de 12,72ºBrix a 16,07ºBrix, a acidez titulável reduziu ao longo do armazenamento, já o pH apresentou variação significativa a partir de 40 dias entre os sistemas utilizados. A relação SS/AT alcançou os melhores índices no SAUO até 40 dias, já a firmeza de polpa e a perda de massa apresentaram redução nos SAC e SAUO, contrastando com elevada coloração da polpa. Açúcares redutores e não redutores apresentaram redução no SAC e SAUO aos 30 e 40 dias de armazenamento, já para os compostos fenólicos e atividade antioxidante, nestes sistemas, com 30, 40 e 50 dias os valores mantiveram-se semelhantes. Os carotenóides foram mantidos até 40 dias no SAUO. O SAC manteve os melhores parâmetros de qualidade físico-químicos analisados, juntamente com SAUO, sendo ambos os mais indicados. Os compostos fitoquímicos analisados neste estudo apresentaram poucas alterações durante os dias de armazenamento nos sistemas analisados.(AU)

A hierarchical state estimation and control framework for monitoring and dissolved oxygen regulation in bioprocesses.

The integration of state estimation and control is a promising approach to overcome challenges related to unavailable or noisy online measurements and plant-model mismatch. Extended Kalman filter (EKF) and moving horizon estimator (MHE) are widely used methods that have complementary features. EKF provides fast estimation and MHE optimal performance. In this paper, a novel hierarchical EKF/MHE approach combined with a dynamic matrix controller (DMC), denoted as EKF/MHE-DMC, is proposed for process monitoring and dissolved oxygen control in airlift bioreactors. The approach is successfully tested in simulated cultivations of Escherichia coli for recombinant protein production, considering specific scenarios of step set point tracking, step disturbance rejection, plant-model mismatch, and measurement noise. Results also show that, given a model that describes the measured dissolved oxygen precisely, as assumed in this study for the in silico experiments, the EKF/MHE-DMC approach is able to estimate the cell, protein, substrate, and dissolved oxygen concentrations based only on the measurement of the latter, reducing the estimation error by 93.8% when compared to a benchmark case employing EKF and DMC. The general structure of the proposed EKF/MHE-DMC framework could be adapted for implementation on other relevant bioprocess systems employing their derived process models.

Different responses in the expression of alginases, alginate polymerase and acetylation genes during alginate production by Azotobacter vinelandii under oxygen-controlled conditions.

Alginate production and gene expression of genes involved in alginate biosynthesis were evaluated in continuous cultures under dissolved oxygen tension (DOT) controlled conditions. Chemostat at 8% DOT showed an increase in the specific oxygen uptake rate [Formula: see text] from 10.9 to 45.3 mmol g-1 h-1 by changes in the dilution rate (D) from 0.06 to 0.10 h-1, whereas under 1% DOT the [Formula: see text] was not affected. Alginate molecular weight was not affected by DOT. However, chemostat at 1% DOT showed a downregulation up to 20-fold in genes encoding both the alginate polymerase (alg8, alg44), alginate acetylases (algV, algI) and alginate lyase AlgL. alyA1 and algE7 lyases gene expressions presented an opposite behavior by changing the DOT, suggesting that A. vinelandii can use specific depolymerases depending on the oxygen level. Overall, the DOT level have a differential effect on genes involved in alginate synthesis, thus a gene expression equilibrium determines the production of alginates of similar molecular weight under DOT controlled.