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1.
Plants (Basel) ; 12(5)2023 Feb 24.
Article in English | MEDLINE | ID: mdl-36903893

ABSTRACT

The Brazilian semiarid region stands out in terms of sour passion fruit production. Local climatic conditions (high air temperature and low rainfall), combined with its soil properties (rich in soluble salts), increase salinity effects on plants. This study was carried out in the experimental area "Macaquinhos" in Remígio-Paraíba (Brazil). The aim of this research was to evaluate the effect of mulching on grafted sour passion fruit under irrigation with moderately saline water. The experiment was conducted in split-plots in a 2 × (2 × 2) factorial scheme to evaluate the effects of the combination of irrigation water salinity of 0.5 dS m-1 (control) and 4.5 dS m-1 (main plot), passion fruit propagated by seed and grafted onto Passiflora cincinnata, with and without mulching (subplots), with four replicates and three plants per plot. The foliar Na concentration in grafted plants was 90.9% less than that of plants propagated via seeds; however, it did not affect fruit production. Plastic mulching, by reducing the absorption of toxic salts and promoting greater absorption of nutrients, contributed to greater production of sour passion fruit. Under irrigation with moderately saline water, the plastic film in the soil and seed propagation promote higher production of sour passion fruit.

2.
Semina ciênc. agrar ; 42(1): 137-154, jan.-fev. 2021. tab, graf
Article in English | VETINDEX | ID: biblio-1501912

ABSTRACT

This study was conducted to evaluate the gas exchange, growth, and quality of passion fruit cultivars under irrigation with waters of different salinity levels. The experiment was conducted in a greenhouse, in Pombal-PB, Brazil, using a randomized block design in a 5 ? 2 factorial arrangement, with five levels of electrical conductivity of irrigation water (0.3, 1.1, 1.9, 2.7, and 3.5 dS m-1) and two passion fruit cultivars (BRS Sol do Cerrado and Guinezinho), with three plants per plot and four replicates. The reduction in transpiration, intercellular CO2 concentration and CO2 assimilation rate in passion fruit plants grown with saline water was related to factors of stomatal and non-stomatal origin. Gas exchange and growth of passion fruit cultivars were negatively affected by water salinity from 0.3 dS m-1 at 75 days after sowing. Despite the reduction in the growth of the passion fruit cultivars, irrigation with water of up to 3.5 dS m-1 could be used to obtain seedlings with acceptable quality. Based on the relative yield of total dry phytomass, the passion fruit cultivars BRS Sol do Cerrado and Guinezinho were classified as moderately sensitive to water salinity during the seedling phase.


Desenvolveu-se este trabalho com o objetivo de avaliar as trocas gasosas, o crescimento e a qualidade de cultivares de maracujazeiro sob irrigação com águas de diferentes níveis salinos. O experimento foi conduzido em casa de vegetação, em Pombal-PB, utilizando-se o delineamento de blocos casualizados em arranjo fatorial 5 × 2, sendo cinco níveis de condutividade elétrica da água de irrigação (0,3; 1,1; 1,9; 2,7 e 3,5 dS m-1) e duas cultivares de maracujazeiro (BRS Sol do Cerrado e Guinezinho), com três plantas por parcela e quatro repetições. A redução na transpiração, concentração intercelular de CO2 e taxa de assimilação de CO2 nas plantas de maracujazeiro cultivadas com águas salinas está relacionado a fatores de origem estomáticos e não estomáticos. As trocas gasosas e o crescimento das cultivares de maracujazeiro foram afetados de forma negativa pela salinidade da água a partir de 0,3 dS m-1, aos 75 dias após o semeio. Apesar da redução no crescimento nas cultivares de maracujazeiro, a irrigação com água de até 3,5 dS m-1 pode ser utilizada para obtenção de mudas com qualidade aceitável. Com base no rendimento relativo de fitomassa seca total, as cultivares de maracujazeiro BRS Sol do Cerrado e Guinezinho foram classificados como moderadamente sensível a salinidade da água na fase de formação de mudas.


Subject(s)
Salt Stress , Molting/physiology , Passiflora/physiology , Passiflora/chemistry , Saline Waters/analysis
3.
Semina Ci. agr. ; 42(1): 137-154, jan.-fev. 2021. tab, graf
Article in English | VETINDEX | ID: vti-31479

ABSTRACT

This study was conducted to evaluate the gas exchange, growth, and quality of passion fruit cultivars under irrigation with waters of different salinity levels. The experiment was conducted in a greenhouse, in Pombal-PB, Brazil, using a randomized block design in a 5 ? 2 factorial arrangement, with five levels of electrical conductivity of irrigation water (0.3, 1.1, 1.9, 2.7, and 3.5 dS m-1) and two passion fruit cultivars (BRS Sol do Cerrado and Guinezinho), with three plants per plot and four replicates. The reduction in transpiration, intercellular CO2 concentration and CO2 assimilation rate in passion fruit plants grown with saline water was related to factors of stomatal and non-stomatal origin. Gas exchange and growth of passion fruit cultivars were negatively affected by water salinity from 0.3 dS m-1 at 75 days after sowing. Despite the reduction in the growth of the passion fruit cultivars, irrigation with water of up to 3.5 dS m-1 could be used to obtain seedlings with acceptable quality. Based on the relative yield of total dry phytomass, the passion fruit cultivars BRS Sol do Cerrado and Guinezinho were classified as moderately sensitive to water salinity during the seedling phase.(AU)


Desenvolveu-se este trabalho com o objetivo de avaliar as trocas gasosas, o crescimento e a qualidade de cultivares de maracujazeiro sob irrigação com águas de diferentes níveis salinos. O experimento foi conduzido em casa de vegetação, em Pombal-PB, utilizando-se o delineamento de blocos casualizados em arranjo fatorial 5 × 2, sendo cinco níveis de condutividade elétrica da água de irrigação (0,3; 1,1; 1,9; 2,7 e 3,5 dS m-1) e duas cultivares de maracujazeiro (BRS Sol do Cerrado e Guinezinho), com três plantas por parcela e quatro repetições. A redução na transpiração, concentração intercelular de CO2 e taxa de assimilação de CO2 nas plantas de maracujazeiro cultivadas com águas salinas está relacionado a fatores de origem estomáticos e não estomáticos. As trocas gasosas e o crescimento das cultivares de maracujazeiro foram afetados de forma negativa pela salinidade da água a partir de 0,3 dS m-1, aos 75 dias após o semeio. Apesar da redução no crescimento nas cultivares de maracujazeiro, a irrigação com água de até 3,5 dS m-1 pode ser utilizada para obtenção de mudas com qualidade aceitável. Com base no rendimento relativo de fitomassa seca total, as cultivares de maracujazeiro BRS Sol do Cerrado e Guinezinho foram classificados como moderadamente sensível a salinidade da água na fase de formação de mudas.(AU)


Subject(s)
Passiflora/chemistry , Passiflora/physiology , Saline Waters/analysis , Salt Stress , Molting/physiology
4.
rev. udca actual. divulg. cient ; 23(1): e1303, ene.-jun. 2020. tab, graf
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1127544

ABSTRACT

RESUMEN El maracuyá es un fruto tropical que se cultiva primordialmente para la obtención de pulpa, siendo la corteza su principal subproducto que carece, en la actualidad, de interés industrial; sin embargo, con el fin de diversificar los derivados provenientes de la producción de maracuyá, se ha demostrado que la corteza contiene carotenoides, dentro de los que se destaca el β-caroteno y la luteína, que podrían ser utilizados en alimentos, debido a su poder antioxidante e, incluso, como colorante natural alternativo. Por lo anterior, el objetivo de este trabajo fue obtener un extracto rico en carotenoides a partir de su corteza; para ello, se evaluó la extracción con etanol, mediante tres técnicas: inmersión, baño termostático y soxhlet. El mayor rendimiento, se obtuvo con el método soxhlet; para esta técnica, se estudió el efecto de la concentración de etanol (entre 80% y 90% v/v), la proporción solvente-materia prima (con relaciones entre 40:1 y 50:1) y el tiempo (definido entre 90 y 150 minutos). Los resultados, se analizaron mediante modelo de superficie de respuesta, obteniendo el mayor rendimiento con etanol al 90%, utilizando 50mL/g corteza y 150 minutos de operación. A estas condiciones, el rendimiento de extracción fue de 2208,53µg β-caroteno/100g muestra. Por último, se determinó la diferencia de color entre el extracto óptimo y una solución de tartrazina, evidenciando una diferencia de color de 3,07 unidades cieLAB, lo cual, muestra que el producto de lixiviación de la corteza de maracuyá tiene potencial para su uso como aditivo alimentario, reemplazando colorantes sintéticos, como la tartrazina.


ABSTRACT Passion fruit is a tropical fruit that is usually cultivated to obtain pulp, being the cortex its main not exploited by-product, because of the actual absence of industrial interest. However, with the aim of diversifying the derivates from the production of passion fruit products, it has been demonstrated that the cortex contains carotenoids, such as β-carotene and lutein; which could be used in food due to its antioxidant capacity, or even like a food colorant. Therefore, the objective of this work was to obtain a carotenoid-rich extract from its cortex; for this, three techniques of extraction with ethanol were evaluated, by immersion, thermostatic bath and Soxhlet. Being the last technique that one with which the best yield was obtained. With the highest yield technique, the following factors were evaluated: ethanol concentration on 80% and 90% v/v, solvent-raw material ratio on 40:1 and 50:1 and time on 90 and 150min. The best yield was obtaining to 90% ethanol, 50mL solvent/g raw material and 150min, for an extraction yield of 2208.53µg β-caroten/100 g sample. Finally, the color difference between the optimal extract and a tartrazine solution was determined, evidencing a color difference of 3.07cieLAB units; this shows that the passion fruit leaching product has the potential to be used as a food additive, replacing synthetic dyes such as tartrazine.

5.
Food Chem Toxicol ; 118: 557-565, 2018 Aug.
Article in English | MEDLINE | ID: mdl-29886231

ABSTRACT

Antiinflammatory and antitumor activity has been reported in Passiflora edulis (yellow passion fruit) nevertheless the intrinsic mechanisms of action are not fully elucidated. The present study aimeds to perform a comparison between the antitumor activity involving the crude extract (HCE) and the supercritical fluid extract with ethanol as co-solvent (SFEtOH) from P. edulis f. flavicarpa Deg. The in vitro cytotoxicity was evaluated in MCF-7 cells, while the in vivo antitumor activity was assessed in male Balb/c mice inoculated with Ehrlich carcinoma cells. SFEtOH exhibited higher antitumor activity compared to HCE. Wherein, SFEtOH showed an EC50 of 264.6 µg/mL against MCF-7 cells as well as an increased inhibition of tumor growth of 48.5% (p < 0.001) in male Balb/c mice, thereby promoting an increased mice lifespan to approximately 42%. Moreover, SFEtOH caused lipid (p < 0.001) and protein (p < 0.001) oxidation by increasing glutathione redox cycle activity while decreased the thioredoxin reductase activity (p < 0.001). SFEtOH also induced oxidative DNA damage in Ehrlich ascites carcinoma (EAC) cells leading to G2/M cycle arrest and has increased apoptotic cells up to 48.2%. These data suggest that the probable mechanisms of antitumor effect are associated to the lipid, protein and DNA damage, leading to cell cycle arrest and triggering apoptosis via mitochondrial pathway, should be probable due to the presence of medium and long chain fatty acids such as lauric acid.


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Apoptosis/drug effects , Cell Cycle Checkpoints/drug effects , Fatty Acids/metabolism , Oxidative Stress , Passiflora/chemistry , Animals , Antineoplastic Agents, Phytogenic/isolation & purification , Biomarkers , Drug Screening Assays, Antitumor , Gas Chromatography-Mass Spectrometry , Humans , MCF-7 Cells , Male , Mice, Inbred BALB C , Xenograft Model Antitumor Assays
6.
Food Chem ; 219: 1-6, 2017 Mar 15.
Article in English | MEDLINE | ID: mdl-27765203

ABSTRACT

Thermal food processing mainly aims to control microorganism in order to extend its shelf life. However, it may induce chemical and nutritional changes in foodstuff. The Nuclear Magnetic Resonance (NMR) coupled to multivariate analysis was used to evaluate the effect of different thermal processing conditions (85 and 140°C for 4; 15; 30; and 60s) on the passion fruit juice using an Armfield pasteurizer. Through this approach it was possible to identify the changes in the juice composition. The temperature and the time lead to a hydrolysis of the sucrose to glucose and fructose. Additionally, juice submitted to 140°C for 60s results in the degradation of the sucrose and the formation of 5-(hydroxymethyl)-2-furfural (HMF). Despite no novel chemical marker has been identified, the 1H NMR chemometrics approach may contribute in the choice of the temperature and time to be employed in the juice processing.


Subject(s)
Fruit and Vegetable Juices/analysis , Magnetic Resonance Spectroscopy/methods , Temperature , Food Handling , Maillard Reaction
7.
Rev. colomb. biotecnol ; 18(2): 56-65, jul.-dic. 2016. ilus, tab
Article in Spanish | LILACS | ID: biblio-959840

ABSTRACT

El maracuyá amarillo es un cultivo de importancia económica para el Valle del Cauca, pero en los últimos años ha presentado una reducción de hasta un 80% en la producción debido a problemas virales. El objetivo del presente trabajo fue detectar y caracterizar parcialmente begomovirus que podrían estar afectando los cultivos de maracuyá amarillo localizados en dos zonas productoras del Valle del Cauca (La Unión y Palmira). Se colectaron hojas de maracuyá con síntomas típicos de enfermedad viral, se purificó su DNA genómico y se detectó la presencia de begomovirus bipartitas mediante PCR empleando cebadores específicos para el componente A y B, respectivamente. Cinco fragmentos de DNA correspondientes al genoma geminiviral A y B, fueron clonados, secuenciados y analizados con herramientas bioinformáticas. Los resultados evidencian por primera vez en Colombia de la presencia de un begomovirus en maracuyá amarillo. El análisis de la secuencias de nucleótidos indica que este begomovirus está más relacionado con el virus del mosaico enano del frijol, un geminivirus que afecta frijol en Colombia, y no con el virus de la distorsión severa de la hoja de maracuyá, el cual se reportó afectando maracuyá amarillo en Brasil. A la fecha, este resultado constituiría el primer reporte de un begomovirus (perteneciente a la familia Geminiviridae) que afecta maracuyá amarillo en Colombia y que podría ser diferente de otros begomovirus previamente reportados a nivel mundial afectando este cultivo.


The yellow passion fruit is a crop of economic importance to the Valle del Cauca, but in recent years has been a reduction of up to 80% in production due to viral problems. The aim of this study was to detect and characterize partially begomovirus that could be affecting crops of yellow passionfruit located in two growing areas of the Valle del Cauca (La Union and Palmira). Passionfruit leaves with typical symptoms of viral disease were collected, purified genomic DNA and its bipartite begomovirus were detected by PCR using primers specific for component A and B, respectively. Five DNA fragments corresponding to geminiviral A and B genome were cloned, sequenced and analyzed with bioinformatics tools. The results show for the first time in Colombia the presence of a begomovirus in yellow passionfruit. The analysis of the nucleotide sequences indicates that this begomovirus is more related to bean BDMV, a geminivirus affecting beans in Colombia, not PSLDV, which was reported affecting yellow passionfruit in Brazil. To date, this result would be the first report of a begomovirus (belonging to the family Geminiviridae) affecting yellow passion fruit in Colombia, and that might be different from other begomovirus previously reported worldwide affecting this crop.

8.
R. Ci. agrovet. ; 15(1): 3-7, 2016. tab
Article in Portuguese | VETINDEX | ID: vti-690827

ABSTRACT

Alelopatia se refere à capacidade que determinada planta possui de interferir no metabolismo de outra, por meio de compostos químicos liberados no meio. A tiririca é um exemplo típico deste tipo de planta, possuindo elevada concentração de ácido indolbutírico, podendo agir na germinação e enraizamento de outras plantas. Diante do exposto, objetivou-se com o presente trabalho avaliar o efeito de extratos de bulbos e folhas de tiririca e ácido giberélico na germinação de sementes de maracujá-amarelo. Para a obtenção dos extratos aquosos de tiririca utilizaram-se bulbos e folhas na concentração de 1 g 10 mL-1 (p/v) (1 g de matéria vegetal para 10 mL de água destilada), constituindo as soluções estoque. Foram utilizados extratos diluídos em cinco concentrações (20, 40, 60, 80 e 100%) e cinco concentrações de ácido giberélico (200, 400, 600, 800 e 1000 mg L-1 GA3 ). Como tratamento controle utilizou-se água destilada. O delineamento experimental foi inteiramente casualizado, apresentando 16 tratamentos, contendo cinco repetições, totalizando 80 unidades amostrais, contendo cada unidade dez sementes. Diariamente realizaram-se contagens do número de sementes germinadas para posterior cálculo de percentagem de germinação e Índice de Velocidade de Germinação. Sementes de maracujá-amarelo submetidas às concentrações de ácido giberélico tiveram sua germinação inibida, não sendo recomendado na germinação da espécie. Concentrações de extrato aquoso de folhas e bulbos de tiririca não influenciaram a germinação de sementes de maracujá-amarelo.(AU)


Allelopathy refers to the ability of certain plant has to interfere with the metabolism of other compounds by chemicals released into the environment. Cyperus rotundus is a typical example of such a plant, having a high IBA concentration and being able to influence and act on germination and rooting of the other plants. The aim of this study was to evaluate the effect of bulbs and leaves extracts of Cyperus rotundus and gibberellic acid on the yellow passion fruit germination cycle. To obtain the aqueous extract bulbs and leaves were used along with a concentration of 1 g 10 mL-1 (p/v) (1 g of material plant to 10 mL of distilled water), forming stock solutions. Extracts were diluted in five concentrations (20, 40, 60, 80 and 100%) and five concentrations of gibberellic acid (200, 400, 600, 800 and 1000 mg L-1 GA3 ) were used. As a control treatment we used distilled water. The experimental design was completely randomized, with 16 treatments, five replicates, totaling 80 sampling units, each unit containing ten seeds. Daily counts were performed on the number of germinated seeds for subsequent calculation of germination percentage and germination speed index. Seeds of yellow passion fruit treated with concentrations of gibberellic acid had their germination inhibited and it is not recommended in the germination of the species. Concentrations of aqueous extract of leaves and sedge did not affect the germination of yellow passion fruit.(AU)


Subject(s)
Germination/physiology , Passiflora , Allelopathy/physiology , Plant Extracts/analysis , Cyperus , Plant Development/physiology
9.
Rev. Ciênc. Agrovet. (Online) ; 15(1): 3-7, 2016. tab
Article in Portuguese | VETINDEX | ID: biblio-1488112

ABSTRACT

Alelopatia se refere à capacidade que determinada planta possui de interferir no metabolismo de outra, por meio de compostos químicos liberados no meio. A tiririca é um exemplo típico deste tipo de planta, possuindo elevada concentração de ácido indolbutírico, podendo agir na germinação e enraizamento de outras plantas. Diante do exposto, objetivou-se com o presente trabalho avaliar o efeito de extratos de bulbos e folhas de tiririca e ácido giberélico na germinação de sementes de maracujá-amarelo. Para a obtenção dos extratos aquosos de tiririca utilizaram-se bulbos e folhas na concentração de 1 g 10 mL-1 (p/v) (1 g de matéria vegetal para 10 mL de água destilada), constituindo as soluções estoque. Foram utilizados extratos diluídos em cinco concentrações (20, 40, 60, 80 e 100%) e cinco concentrações de ácido giberélico (200, 400, 600, 800 e 1000 mg L-1 GA3 ). Como tratamento controle utilizou-se água destilada. O delineamento experimental foi inteiramente casualizado, apresentando 16 tratamentos, contendo cinco repetições, totalizando 80 unidades amostrais, contendo cada unidade dez sementes. Diariamente realizaram-se contagens do número de sementes germinadas para posterior cálculo de percentagem de germinação e Índice de Velocidade de Germinação. Sementes de maracujá-amarelo submetidas às concentrações de ácido giberélico tiveram sua germinação inibida, não sendo recomendado na germinação da espécie. Concentrações de extrato aquoso de folhas e bulbos de tiririca não influenciaram a germinação de sementes de maracujá-amarelo.


Allelopathy refers to the ability of certain plant has to interfere with the metabolism of other compounds by chemicals released into the environment. Cyperus rotundus is a typical example of such a plant, having a high IBA concentration and being able to influence and act on germination and rooting of the other plants. The aim of this study was to evaluate the effect of bulbs and leaves extracts of Cyperus rotundus and gibberellic acid on the yellow passion fruit germination cycle. To obtain the aqueous extract bulbs and leaves were used along with a concentration of 1 g 10 mL-1 (p/v) (1 g of material plant to 10 mL of distilled water), forming stock solutions. Extracts were diluted in five concentrations (20, 40, 60, 80 and 100%) and five concentrations of gibberellic acid (200, 400, 600, 800 and 1000 mg L-1 GA3 ) were used. As a control treatment we used distilled water. The experimental design was completely randomized, with 16 treatments, five replicates, totaling 80 sampling units, each unit containing ten seeds. Daily counts were performed on the number of germinated seeds for subsequent calculation of germination percentage and germination speed index. Seeds of yellow passion fruit treated with concentrations of gibberellic acid had their germination inhibited and it is not recommended in the germination of the species. Concentrations of aqueous extract of leaves and sedge did not affect the germination of yellow passion fruit.


Subject(s)
Allelopathy/physiology , Cyperus , Plant Extracts/analysis , Germination/physiology , Passiflora , Plant Development/physiology
10.
Ci. Rural ; 45(2): 356-363, 02/2015. tab, graf
Article in English | VETINDEX | ID: vti-12327

ABSTRACT

The profile of volatile compounds of yellow passion fruit juice was analyzed by solid phase microextraction headspace (HS-SPME) and optimized static headspace (S-HS) extraction techniques. Time, temperature, NaCl concentration and sample volume headspace equilibrium parameters was adjusted to the S-HS technique. The gaseous phase in the headspace of samples was collected and injected into a gas chromatograph coupled to a mass spectrometer. In the HS-SPME technique was identified 44 volatile compounds from the yellow passion fruit juice, but with S-HS only 30 compounds were identified. Volatile esters were majority in both techniques, being identified ethyl butanoate, ethyl hexanoate, (3z)-3-hexenyl acetate, hexyl acetate, hexyl butanoate and hexyl hexanoate. Aldehydes and ketones were not identified in S-HS, but were in HS-SPME. β-Pinene, p-cymene, limonene, (Z)-β-ocimene, (E)-β-ocimene, γ-terpinene, α-terpinolene and (E) -4,8-dimethyl-1, 3,7 - nonatriene terpenes were identified in both techniques. This study showed that the S-HS optimized extraction technique was effective to recovery high concentrations of the major volatile characteristics compounds in the passion fruit, such as ethyl butanoate and ethyl hexanoate, which can be advantageous due to the simplicity of the method.(AU)


O perfil de compostos voláteis do suco de maracujá-amarelo foi analisado pelas técnicas de extração microextração em fase sólida (SPME-HS) e headspace estático (S-HS). Os parâmetros de equilíbrio do headspace tempo, temperatura, concentração de NaCl e volume da amostra foram ajustados para a técnica S-HS. Em ambas as técnicas de extração, a fase gasosa no headspace das amostras foi recolhida e injetada em cromatógrafo gasoso acoplado a espectrômetro de massas. Na técnica SPME-HS, foram identificados 44 compostosvoláteis no suco do maracujá-amarelo e, em S-HS, foram identificados 30 compostos. Ésteres voláteis foram majoritários em ambas as técnicas, sendo identificados butanoato de etila, hexanoato de etila, (3z)-3-hexenil acetato, hexil acetato, hexil butanoatoe hexil hexanoato. Aldeídos e cetonas não foram identificados com a técnica S-HS. Os terpenos β-pineno, p-cimeno, limoneno, (Z)-β-ocimeno, (E)-β-ocimeno, γ-terpineno, α-terpinoleno e (E)-4,8-dimetil-1,3,7-nonatrieno foram identificados em ambas as tιcnicas.. Este estudo mostrou que a técnica de extração otimizada S-HS foi eficaz para a recuperação de altas concentrações dos principais compostos voláteis característicos do maracujá, como o butanoato de etila e o hexanoate deetila, o que é vantajoso, devido à simplicidade do método.(AU)


Subject(s)
Passiflora/chemistry , Juices , Solid Phase Microextraction/methods , Chemistry Techniques, Analytical , Esters , Terpenes
11.
Ciênc. rural ; Ciênc. rural (Online);45(2): 356-363, 02/2015. tab, graf
Article in English | LILACS | ID: lil-732379

ABSTRACT

The profile of volatile compounds of yellow passion fruit juice was analyzed by solid phase microextraction headspace (HS-SPME) and optimized static headspace (S-HS) extraction techniques. Time, temperature, NaCl concentration and sample volume headspace equilibrium parameters was adjusted to the S-HS technique. The gaseous phase in the headspace of samples was collected and injected into a gas chromatograph coupled to a mass spectrometer. In the HS-SPME technique was identified 44 volatile compounds from the yellow passion fruit juice, but with S-HS only 30 compounds were identified. Volatile esters were majority in both techniques, being identified ethyl butanoate, ethyl hexanoate, (3z)-3-hexenyl acetate, hexyl acetate, hexyl butanoate and hexyl hexanoate. Aldehydes and ketones were not identified in S-HS, but were in HS-SPME. β-Pinene, p-cymene, limonene, (Z)-β-ocimene, (E)-β-ocimene, γ-terpinene, α-terpinolene and (E) -4,8-dimethyl-1, 3,7 - nonatriene terpenes were identified in both techniques. This study showed that the S-HS optimized extraction technique was effective to recovery high concentrations of the major volatile characteristics compounds in the passion fruit, such as ethyl butanoate and ethyl hexanoate, which can be advantageous due to the simplicity of the method.


O perfil de compostos voláteis do suco de maracujá-amarelo foi analisado pelas técnicas de extração microextração em fase sólida (SPME-HS) e headspace estático (S-HS). Os parâmetros de equilíbrio do headspace tempo, temperatura, concentração de NaCl e volume da amostra foram ajustados para a técnica S-HS. Em ambas as técnicas de extração, a fase gasosa no headspace das amostras foi recolhida e injetada em cromatógrafo gasoso acoplado a espectrômetro de massas. Na técnica SPME-HS, foram identificados 44 compostosvoláteis no suco do maracujá-amarelo e, em S-HS, foram identificados 30 compostos. Ésteres voláteis foram majoritários em ambas as técnicas, sendo identificados butanoato de etila, hexanoato de etila, (3z)-3-hexenil acetato, hexil acetato, hexil butanoatoe hexil hexanoato. Aldeídos e cetonas não foram identificados com a técnica S-HS. Os terpenos β-pineno, p-cimeno, limoneno, (Z)-β-ocimeno, (E)-β-ocimeno, γ-terpineno, α-terpinoleno e (E)-4,8-dimetil-1,3,7-nonatrieno foram identificados em ambas as tιcnicas.. Este estudo mostrou que a técnica de extração otimizada S-HS foi eficaz para a recuperação de altas concentrações dos principais compostos voláteis característicos do maracujá, como o butanoato de etila e o hexanoate deetila, o que é vantajoso, devido à simplicidade do método.

12.
Ci. Rural ; 44(2): 235-240, fev. 2014. tab
Article in Portuguese | VETINDEX | ID: vti-29201

ABSTRACT

Objetivou-se avaliar indicadores energéticos de um novo sistema de produção do maracujá amarelo na região de Marília-SP. Foram analisadas as "entradas" de energia de origem biológica, fóssil e industrial e a "saída" energética na forma de frutos produzidos por unidade de área, bem como cinco indicadores energéticos. A entrada energética totalizou 155.810,13 MJ/ha. Os adubos químicos e defensivos responderam por 71% e 24% da energia indireta industrial, respectivamente. A atividade gerou, por ciclo/ha, 587.700,00MJ, sendo a produtividade cultural 0,19MJ kg-1. A eficiência cultural foi de 3,77. A energia cultural líquida totalizou 431.889,87 MJ/ha. A eficiência energética (4.17) e o balanço energético (550.312,91MJ ha-1) foram positivos. Concluiu-se que o consumo de energia direta de origem fóssil, mesmo que significativo, ainda permite a produção do fruto de maneira ambientalmente sustentável. Os adubos químicos e defensivos foram os itens mais representativos da energia indireta industrial, em decorrência da intensificação da adubação como forma de prevenção de doenças.(AU)


The study aimed to evaluate energy indicators of a new production system of yellow passion fruit in Marília-SP. Analyzed the "inputs" of the biological energy, fossil and industrial and "output" energy in the form of fruits produced per unit area as well as five energy indicators. The input energy amounted 155,810.13MJ ha-1. Chemical fertilizers and pesticides accounted for 71% and 24% of indirect energy industry, respectively. The activity generated per cycle / ha, 587.700.00MJ, and cultural productivity 0.19MJ kg-1. The efficiency culture was 3.77. The cultural energy net totaled 431,889.87MJ ha-1. The energy efficiency (4.17) and energy balance (550,312.91MJ ha-1) was favorable. It was concluded that the direct energy consumption from fossil fuels, even though significant, yet permits the production of the fruit of an environmentally sustainable manner. Chemical fertilizers and pesticides were the most representative of indirect energy industry due to the intensification of fertilization as a means of disease prevention.(AU)


Subject(s)
Passiflora , 24444 , Energy Consumption/economics
13.
Ciênc. rural ; Ciênc. rural (Online);44(2): 235-240, fev. 2014. tab
Article in Portuguese | LILACS | ID: lil-701364

ABSTRACT

Objetivou-se avaliar indicadores energéticos de um novo sistema de produção do maracujá amarelo na região de Marília-SP. Foram analisadas as "entradas" de energia de origem biológica, fóssil e industrial e a "saída" energética na forma de frutos produzidos por unidade de área, bem como cinco indicadores energéticos. A entrada energética totalizou 155.810,13 MJ/ha. Os adubos químicos e defensivos responderam por 71% e 24% da energia indireta industrial, respectivamente. A atividade gerou, por ciclo/ha, 587.700,00MJ, sendo a produtividade cultural 0,19MJ kg-1. A eficiência cultural foi de 3,77. A energia cultural líquida totalizou 431.889,87 MJ/ha. A eficiência energética (4.17) e o balanço energético (550.312,91MJ ha-1) foram positivos. Concluiu-se que o consumo de energia direta de origem fóssil, mesmo que significativo, ainda permite a produção do fruto de maneira ambientalmente sustentável. Os adubos químicos e defensivos foram os itens mais representativos da energia indireta industrial, em decorrência da intensificação da adubação como forma de prevenção de doenças.


The study aimed to evaluate energy indicators of a new production system of yellow passion fruit in Marília-SP. Analyzed the "inputs" of the biological energy, fossil and industrial and "output" energy in the form of fruits produced per unit area as well as five energy indicators. The input energy amounted 155,810.13MJ ha-1. Chemical fertilizers and pesticides accounted for 71% and 24% of indirect energy industry, respectively. The activity generated per cycle / ha, 587.700.00MJ, and cultural productivity 0.19MJ kg-1. The efficiency culture was 3.77. The cultural energy net totaled 431,889.87MJ ha-1. The energy efficiency (4.17) and energy balance (550,312.91MJ ha-1) was favorable. It was concluded that the direct energy consumption from fossil fuels, even though significant, yet permits the production of the fruit of an environmentally sustainable manner. Chemical fertilizers and pesticides were the most representative of indirect energy industry due to the intensification of fertilization as a means of disease prevention.

14.
Biosci. j. (Online) ; 30(3 Supplement): 293-302, 2014. tab, graf
Article in English | LILACS | ID: biblio-947731

ABSTRACT

Water is a limiting factor in agricultural activity and its deficiency in the soil affects the physiological processes of the plant and later interferes in the production of the culture. Aiming to evaluate the influence of different rates of reference evapotranspiration (ETo) replacement, over the leaf gas exchange and yield of hybrid yellow passionfruit plants, this experiment was carried out from August/2009 to September/2010, in an area of the semiarid region of the state of Paraiba, Brazil. The treatments consisted of the combination of four levels of irrigation (33, 66, 100 and 133% of ETo replacement) and two hybrid yellow passion fruit species ('BRS Sol do Cerrado' and 'BRS Gigante Amarelo'), in an experimental randomized block design, with five replications. The following variables were evaluated: gas exchange ­ stomatal conductance (gs), transpiration (T), net photosynthesis (A), instantaneous water use efficiency (IWUE), internal carbon concentration (Ci) and instantaneous carboxylation efficiency (A/Ci); fruit weight (distributed into three classes: 'A', 'B' and 'C', per plant) and also, the total production per plant. The gas exchange rates were higher in the 'BRS Sol do Cerrado' hybrid, in which a higher production was registered when irrigated with 133% of the ETo. The 'BRS Gigante Amarelo' genotype is most indicated to grow under low water availability.


A água é um fator limitante à atividade agrícola e sua deficiência no solo afeta os processos fisiológicos da planta, interferindo, posteriormente, na produção da cultura. Com esse enfoque, foi conduzido este experimento em área do semiárido paraibano, no período de agosto/2009 a setembro de 2010, objetivando-se avaliar a influência de diferentes taxas de reposição da ETo, sobre trocas gasosas e rendimento de híbridos de maracujazeiroamarelo. Os tratamentos consistiram da combinação entre quatro lâminas de irrigação (33, 66, 100 e 133% de reposição da ETo) e dois híbridos de maracujazeiro-amarelo ('BRS Sol do Cerrado' e 'BRS Gigante Amarelo'), no delineamento experimental em blocos casualizados, com cinco repetições. Foram avaliados: trocas gasosas - condutância estomática (gs), transpiração (T), taxa de fotossíntese líquida (A), eficiência do uso da água (A/T), concentração de carbono interna (Ci) e eficiência instantânea de carboxilação (A/Ci); peso de frutos (distribuídos em três classes 'A', 'B' e 'C', por planta) e, também, a produção total por planta. As taxas de trocas gasosas foram maiores no híbrido 'BRS Sol do Cerrado', em que se registrou a maior produção, quando irrigado com 133% da ETo. O genótipo 'BRS Gigante Amarelo' é mais indicado para cultivo sob baixa disponibilidade hídrica.


Subject(s)
Photosynthesis , Crops, Agricultural , Passiflora , Agricultural Irrigation
15.
Biosci. j. (Online) ; 30(3 Supplement): 360-370, 2014. tab
Article in Portuguese | LILACS | ID: biblio-947848

ABSTRACT

Dentro do programa de melhoramento de maracujazeiro, a avaliação física, físico-química e molecular dos frutos dos genótipos desenvolvidos é de grande importância, pois permite identificar genótipos com qualidades físico-químicas desejáveis e adaptados para as regiões de cultivo. Sendo assim, objetivou-se avaliar com o presente trabalho as características físicas, físico-químicas, e estimar os parâmetros genéticos de 32 (trinta e dois) genótipos de maracujazeiro-azedo resistente a patógenos cultivados no Distrito Federal. As seguintes características foram analisadas: massa do fruto (g), comprimento (mm), diâmetro (mm), relação comprimento/diâmetro, espessura da casca (mm), massa da casca (g), massa da polpa (g), rendimento de polpa (%), número de sementes, sólidos solúveis totais - SST em °Brix, acidez total titulável - ATT (% de ácido cítrico), pH, relação SST/ATT e cinzas (%). As análises estatísticas e estimativas de parâmetros genéticos foram realizadas com o auxílio dos softwares SISVAR (FERREIRA, 2000) e GENES (CRUZ, 2007). Cerca de 38% dos genótipos avaliados apresentaram características físicas e físico-químicas que se destacaram para utilização destes pela indústria de sucos: pH de 2,7, acidez variando de 5,40 a 3,8 e sólidos solúveis totais de 13,7%. Com relação às características físicas os genótipos que se destacaram foram: 20#49, MSC e planta 7 apresentaram maior comprimento e diâmetro dos frutos. O rendimento de polpa foi superior a 38,7% e os genótipos que se destacaram para essa característica foram: EC-R (46,6%), 20#6 (45,1%), 20#39 (43,6%) e 20#40 (43,4%) sendo que EC-R e 20#6 também apresentaram maior número de sementes por fruto. A massa média dos frutos variou de 226,85 a 128,75g, sendo os maiores valores registrados para os genótipos Planta 7 e EC30. Pela análise dos coeficientes de herdabilidade no sentido amplo ficou evidenciada variância ambiental pronunciada para as características avaliadas.


Within the breeding program of passion, physical assessment, physical chemistry and molecular fruits of genotypes developed is of great importance, since it identifies genotypes with desirable physico-chemical and adapted to the growing regions. Therefore, this study aimed to evaluate the present work the physical, physico-chemical and genetic parameters of 32 (thirty-two) of passion fruit genotypes resistant pathogens grown in the Federal District. The following characteristics were analyzed: fruit mass (g), length (mm) diameter (mm), length / diameter ratio, shell thickness (mm), shell mass (g), pulp mass (g), yield pulp (%), number of seeds, total soluble solids - SST in ° Brix, titratable acidity - ATT (% citric acid), pH, TSS / TA and ash (%). Statistical analyzes and estimates of genetic parameters were performed with the aid of software SISVAR (FERREIRA, 2000) and GENES (CRUZ, 2007). About 38% of the genotypes showed physical and physical-chemical that stood out for use by the industry these juices: pH of 2.7, acidity ranging from 5.40 to 3.8 and total soluble solids of 13.7%. With respect to the physical characteristics of the genotypes that stood out were: 20 # 49, MSC and 7 plant had higher length and diameter. The pulp yield was greater than 38.7% and genotypes that were outstanding for this feature: EC-R (46.6%), 20 # 6 (45.1%) 20 # 39 (43,6% ) # 40 and 20 (43.4%) whereas EC-R and # 20 6 showed a higher number of seeds per fruit. The average fruit weight ranged from 226.85 to 128.75 g, with the highest values recorded for genotypes Plant 7 and EC30. By analyzing the coefficients of broad-sense heritability was evident environmental variance pronounced for traits.


Subject(s)
Crop Production , Passiflora , Plant Breeding , Genotype
16.
Semina Ci. agr. ; 34(1): 227-240, 2013.
Article in Portuguese | VETINDEX | ID: vti-471828

ABSTRACT

 In this work, the contents of phenolic compounds, B-carotene, ascorbic acid and antioxidant activity (DPPH and TEAC methods) were determined in the yellow passion fruit juice during storage at 5 and 24 C, at intervals of 10 days. The fruit quality during storage was determined, and the parameters weight loss, wrinkles, skin color and pathogenic degradation index were used. Samples of fruit at maturity stage with the skin color predominantly green were used for the tests. The results showed that total phenolic compounds, B-carotene and antioxidant activity of the yellow passion fruit juice were not affected by storage temperature and the content of total phenolic compounds of the juice increased with storage time. It as demonstrated that the effects of high temperature during storage is much more evident and impacting the appearance of the fruit, which in the nutritional quality of the juice. The antioxidant activity of juice, expressed in kidnapping DPPH decreased during storage. Based on the microbiological quality, the shelf life of yellow passion fruit was extended to 20 days in refrigerated storage. 


Neste trabalho, os conteúdos de compostos fenólicos totais, B-caroteno, ácido ascórbico e a atividade antioxidante (pelos métodos DPPH e TEAC) foram determinados no suco do maracujá-amarelo durante o armazenamento a 5 e 24 ºC, em intervalos de 10 dias, durante 40 dias. A qualidade dos frutos foi determinada durante o armazenamento, tomando como parâmetros as variáveis perda de massa, enrugamento, cor da casca e o índice de degradação por patógenos. Amostras de frutos em estádio de maturação com a cor da casca predominantemente verde, em início de amarelecimento, foram utilizadas nos ensaios. Os teores de compostos fenólicos totais e B-caroteno, e a atividade antioxidante do suco do maracujá-amarelo não foram influenciados pela temperatura de armazenamento. O conteúdo de compostos fenólicos totais do suco aumentou com o tempo de armazenamento. Os efeitos da temperatura de armazenamento foram mais evidentes e impactantes na aparência do fruto do que na qualidade nutricional do suco. A atividade antioxidante do suco, expressada em seqüestro do radical DPPH, diminuiu durante o armazenamento. Com base na qualidade microbiológica, a vida útil do maracujáamarelo se estendeu até 20 dias no armazenamento refrigerado.

17.
Semina ciênc. agrar ; 34(1): 227-240, 2013.
Article in Portuguese | LILACS-Express | VETINDEX | ID: biblio-1499119

ABSTRACT

In this work, the contents of phenolic compounds, B-carotene, ascorbic acid and antioxidant activity (DPPH and TEAC methods) were determined in the yellow passion fruit juice during storage at 5 and 24 C, at intervals of 10 days. The fruit quality during storage was determined, and the parameters weight loss, wrinkles, skin color and pathogenic degradation index were used. Samples of fruit at maturity stage with the skin color predominantly green were used for the tests. The results showed that total phenolic compounds, B-carotene and antioxidant activity of the yellow passion fruit juice were not affected by storage temperature and the content of total phenolic compounds of the juice increased with storage time. It as demonstrated that the effects of high temperature during storage is much more evident and impacting the appearance of the fruit, which in the nutritional quality of the juice. The antioxidant activity of juice, expressed in kidnapping DPPH decreased during storage. Based on the microbiological quality, the shelf life of yellow passion fruit was extended to 20 days in refrigerated storage.


Neste trabalho, os conteúdos de compostos fenólicos totais, B-caroteno, ácido ascórbico e a atividade antioxidante (pelos métodos DPPH e TEAC) foram determinados no suco do maracujá-amarelo durante o armazenamento a 5 e 24 ºC, em intervalos de 10 dias, durante 40 dias. A qualidade dos frutos foi determinada durante o armazenamento, tomando como parâmetros as variáveis perda de massa, enrugamento, cor da casca e o índice de degradação por patógenos. Amostras de frutos em estádio de maturação com a cor da casca predominantemente verde, em início de amarelecimento, foram utilizadas nos ensaios. Os teores de compostos fenólicos totais e B-caroteno, e a atividade antioxidante do suco do maracujá-amarelo não foram influenciados pela temperatura de armazenamento. O conteúdo de compostos fenólicos totais do suco aumentou com o tempo de armazenamento. Os efeitos da temperatura de armazenamento foram mais evidentes e impactantes na aparência do fruto do que na qualidade nutricional do suco. A atividade antioxidante do suco, expressada em seqüestro do radical DPPH, diminuiu durante o armazenamento. Com base na qualidade microbiológica, a vida útil do maracujáamarelo se estendeu até 20 dias no armazenamento refrigerado.

18.
Arq. Inst. Biol. ; 79(3)2012.
Article in Portuguese | VETINDEX | ID: vti-698641

ABSTRACT

The yellow passion fruit is a major fruit crop in Brazil. The post-harvest preservation of yellow passion fruits has deserved great attention since the fruit is perishable and subject to a fast deterioration and incidence of pathogenic microorganisms. The aim of this work was to evaluate the in vitro antifungal activity of lemon grass derivatives on the mycelial growth, sporulation, spore germination and length of germ tubes of Colletotrichum gloeosporioides and the effect of essential oil, extract, hydrolate and citral lemon grass (Cymbopogon citratus) for control of anthracnose on the yellow passion fruits. The treatments for the in vitro assay were essential oil (OE) (0.5, 1, 5, 10, 15, 25, 50 and 60 µL mL-1), autoclaved crude aqueous extract (EAA), filtered crude aqueous extract (EAF), hydrolate and citral (1, 5, 10, 20 and 25%). For the in vivo assay the fruits were treated with: OE (0.025, 0.05 and 0.1%), hydrolate (5 and 10%), EAA (10%) and citral (0.1%). Distilled water was used as a control. The anthracnose incidence and severity were evaluated every three days. The in vitro test verified that the 4 evaluated derivatives of lemon grass inhibited the mycelial growth, sporulation and spore germination. The higher the concentration of the derivatives, the higher the inhibition, which for the mycelia growth reached 55.8; 54.5; 30.0 and 19.9% for OE, EAF, EAA and hydrolate, respectively. In vivo tests showed that the OE 0.1% and EAA 10% promoted a reduction in the anthracnose severity.


O maracujá-amareloé uma das principais frutíferas cultivadas no Brasil. A conservação pós-colheita de frutos de maracujá tem merecido grande atenção visto que o fruto é perecível e está sujeito à rápida deterioração e incidência de micro-organismos patogênicos. Este trabalho teve por objetivo avaliar a atividade antifúngica in vitro de derivados de capim-limão (Cymbopogon citratus) sobre o crescimento micelial, esporulação, germinação de esporos e tamanho de tubos germinativos de Colletotrichum gloeosporioides e in vivo no controle da antracnose sobre frutos de maracujá-amarelo em pós-colheita. Os tratamentos para o ensaio in vitro foram óleo essencial (OE) (0,5, 1, 5, 10, 15, 25, 50 e 60 µL mL-1), extrato bruto aquoso autoclavado (EAA), extrato bruto aquoso filtrado (EAF), hidrolato e citral (1, 5, 10, 20 e 25%) e no ensaio in vivo, os frutos foram tratados com: OE (0,025; 0,05 e 0,1%), hidrolato (5 e 10%), EAA (10%) e citral (0,1%). Água destilada foi utilizada como testemunha. Foram avaliadas a incidência e a severidade da antracnose a cada três dias. Os quatro derivados de capim-limão inibiram o crescimento micelial, a esporulação e a germinação de esporos, sendo que maiores valores de inibição foram obtidos nas maiores concentrações testadas e, no crescimento micelial, atingiram 55,8; 54,5; 30,0 e 19,9% para OE, EAF, EAA e hidrolato, respectivamente. Na avaliação in vivo, OE 0,1% e EAA 10% promoveram significativa redução na severidade da antracnose.

19.
Arq. Inst. Biol. ; 79(3): 371-379, jul.-set. 2012. graf
Article in Portuguese | VETINDEX | ID: vti-5360

ABSTRACT

O maracujá-amarelo é uma das principais frutíferas cultivadas no Brasil. A conservação póscolheita de frutos de maracujá tem merecido grande atenção visto que o fruto é perecível e está sujeito à rápida deterioração e incidência de micro-organismos patogênicos. Este trabalho teve por objetivo avaliar a atividade antifúngica in vitro de derivados de capim-limão (Cymbopogon citratus) sobre o crescimento micelial, esporulação, germinação de esporos e tamanho de tubos germinativos de Colletotrichum gloeosporioides e in vivo no controle da antracnose sobre frutos de maracujá-amarelo em pós-colheita. Os tratamentos para o ensaio in vitro foram óleo essencial (OE) (0,5, 1, 5, 10, 15, 25, 50 e 60 μL mL-1), extrato bruto aquoso autoclavado (EAA), extrato bruto aquoso filtrado (EAF), hidrolato e citral (1, 5, 10, 20 e 25%) e no ensaio in vivo, os frutos foram tratados com: OE (0,025; 0,05 e 0,1%), hidrolato (5 e 10%), EAA (10%) e citral (0,1%). Água destilada foi utilizada como testemunha. Foram avaliadas a incidência e a severidade da antracnose a cada três dias. Os quatro derivados de capim-limão inibiram o crescimento micelial, a esporulação e a germinação de esporos, sendo que maiores valores de inibição foram obtidos nas maiores concentrações testadas e, no crescimento micelial, atingiram 55,8; 54,5; 30,0 e 19,9% para OE, EAF, EAA e hidrolato, respectivamente. Na avaliação in vivo, OE 0,1% e EAA 10% promoveram significativa redução na severidade da antracnose. (AU)


CONTROL OF ANTHRACNOSE IN YELLOW PASSION FRUIT BY LEMON GRASS (CYMBOPOGON CITRATUS) DERIVATIVES. The yellow passion fruit is a major fruit crop in Brazil. The post-harvest preservation of yellow passion fruits has deserved great attention since the fruit is perishable and subject to a fast deterioration and incidence of pathogenic microorganisms. The aim of this work was to evaluate the in vitro antifungal activity of lemon grass derivatives on the mycelial growth, sporulation, spore germination and length of germ tubes of Colletotrichum gloeosporioides and the effect of essential oil, extract, hydrolate and citral lemon grass (Cymbopogon citratus) for control of anthracnose on the yellow passion fruits. The treatments for the in vitro assay were essential oil (OE) (0.5, 1, 5, 10, 15, 25, 50 and 60 μL mL-1), autoclaved crude aqueous extract (EAA), filtered crude aqueous extract (EAF), hydrolate and citral (1, 5, 10, 20 and 25%). For the in vivo assay the fruits were treated with: OE (0.025, 0.05 and 0.1%), hydrolate (5 and 10%), EAA (10%) and citral (0.1%). Distilled water was used as a control. The anthracnose incidence and severity were evaluated every three days. The in vitro test verified that the 4 evaluated derivatives of lemon grass inhibited the mycelial growth, sporulation and spore germination. The higher the concentration of the derivatives, the higher the inhibition, which for the mycelia growth reached 55.8; 54.5; 30.0 and 19.9% for OE, EAF, EAA and hydrolate, respectively. In vivo tests showed that the OE 0.1% and EAA 10% promoted a reduction in the anthracnose severity. (AU)


Subject(s)
Animals , Distilled Water , Growth/physiology , Crops, Agricultural , Passiflora/classification , Colletotrichum/pathogenicity , Passiflora
20.
Arq. Inst. Biol ; 79(3): 371-379, jul.-set. 2012. graf
Article in Portuguese | VETINDEX | ID: biblio-1462155

ABSTRACT

O maracujá-amarelo é uma das principais frutíferas cultivadas no Brasil. A conservação póscolheita de frutos de maracujá tem merecido grande atenção visto que o fruto é perecível e está sujeito à rápida deterioração e incidência de micro-organismos patogênicos. Este trabalho teve por objetivo avaliar a atividade antifúngica in vitro de derivados de capim-limão (Cymbopogon citratus) sobre o crescimento micelial, esporulação, germinação de esporos e tamanho de tubos germinativos de Colletotrichum gloeosporioides e in vivo no controle da antracnose sobre frutos de maracujá-amarelo em pós-colheita. Os tratamentos para o ensaio in vitro foram óleo essencial (OE) (0,5, 1, 5, 10, 15, 25, 50 e 60 μL mL-1), extrato bruto aquoso autoclavado (EAA), extrato bruto aquoso filtrado (EAF), hidrolato e citral (1, 5, 10, 20 e 25%) e no ensaio in vivo, os frutos foram tratados com: OE (0,025; 0,05 e 0,1%), hidrolato (5 e 10%), EAA (10%) e citral (0,1%). Água destilada foi utilizada como testemunha. Foram avaliadas a incidência e a severidade da antracnose a cada três dias. Os quatro derivados de capim-limão inibiram o crescimento micelial, a esporulação e a germinação de esporos, sendo que maiores valores de inibição foram obtidos nas maiores concentrações testadas e, no crescimento micelial, atingiram 55,8; 54,5; 30,0 e 19,9% para OE, EAF, EAA e hidrolato, respectivamente. Na avaliação in vivo, OE 0,1% e EAA 10% promoveram significativa redução na severidade da antracnose.


CONTROL OF ANTHRACNOSE IN YELLOW PASSION FRUIT BY LEMON GRASS (CYMBOPOGON CITRATUS) DERIVATIVES. The yellow passion fruit is a major fruit crop in Brazil. The post-harvest preservation of yellow passion fruits has deserved great attention since the fruit is perishable and subject to a fast deterioration and incidence of pathogenic microorganisms. The aim of this work was to evaluate the in vitro antifungal activity of lemon grass derivatives on the mycelial growth, sporulation, spore germination and length of germ tubes of Colletotrichum gloeosporioides and the effect of essential oil, extract, hydrolate and citral lemon grass (Cymbopogon citratus) for control of anthracnose on the yellow passion fruits. The treatments for the in vitro assay were essential oil (OE) (0.5, 1, 5, 10, 15, 25, 50 and 60 μL mL-1), autoclaved crude aqueous extract (EAA), filtered crude aqueous extract (EAF), hydrolate and citral (1, 5, 10, 20 and 25%). For the in vivo assay the fruits were treated with: OE (0.025, 0.05 and 0.1%), hydrolate (5 and 10%), EAA (10%) and citral (0.1%). Distilled water was used as a control. The anthracnose incidence and severity were evaluated every three days. The in vitro test verified that the 4 evaluated derivatives of lemon grass inhibited the mycelial growth, sporulation and spore germination. The higher the concentration of the derivatives, the higher the inhibition, which for the mycelia growth reached 55.8; 54.5; 30.0 and 19.9% for OE, EAF, EAA and hydrolate, respectively. In vivo tests showed that the OE 0.1% and EAA 10% promoted a reduction in the anthracnose severity.


Subject(s)
Animals , Growth/physiology , Crops, Agricultural , Distilled Water , Colletotrichum/pathogenicity , Passiflora , Passiflora/classification
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