ABSTRACT
The SARS-CoV-2 worldwide outbreak prompted the development of several tools to detect and treat the disease. Among the new detection proposals, the use of peptides mimetics has surged as an alternative to avoid the use of antibodies, of which there has been a shortage during the COVID-19 pandemic. However, the use of peptides in detection systems still presents some questions to be answered, mainly referring to their stability under different environmental conditions. In this work, we synthesized an ACE2 peptide mimic and evaluated its stability in different pH, salinity, polarity, and temperature conditions. Further, the same conditions were assessed when using the ability of the peptide mimic to detect the recombinant SARS-CoV-2 spike protein in a biotin-streptavidin-enzyme-linked assay. Finally, we also tested the capacity of the peptide to detect SARS-CoV-2 from patients' samples. The results indicate that the peptide is structurally sensitive to the medium conditions, with relevance to the pH, where basic pH favored its performance when used as a SARS-CoV-2 detector. Further, the proposed peptide mimic was able to detect SARS-CoV-2 comparably to RT-qPCR results. Therefore, the present study promotes knowledge advancement, particularly in terms of stability considerations, in the application of peptide mimics as a replacement for antibodies in detection systems.
Subject(s)
COVID-19 , Humans , COVID-19/diagnosis , SARS-CoV-2 , Angiotensin-Converting Enzyme 2 , RNA, Viral , Pandemics , Peptides , Protein BindingABSTRACT
Water's physical and chemical characteristics are important constraints in aquatic ecosystems, acting on the development, survival, and adaptation of different organisms. Immature forms of mosquitoes develop in widely diverse aquatic environments and are mainly found in permanent or temporary freshwater bodies with little or no movement. The current study aimed to investigate whether variations in larval habitats' pH, salinity, dissolved oxygen, and water temperature influence the composition of Culicidae assemblages and the presence and abundance of Aedes albopictus and Ae. aegypti. From August 2012 to July 2013, captures of immature forms and measurement of water's physical and chemical profiles were performed monthly in natural and artificial breeding sites in four urban parks in the city of São Paulo, Brazil. Changes in species composition related to the parameters' variation were assessed by multivariate analysis. Regression trees were performed to evaluate the effect of breeding sites' physical and chemical variations on the presence and abundance of Ae. albopictus and Ae. aegypti. The observations suggest ranges of conditions for the measured variables in which most species tend to be found more frequently, and pH and salinity are the variables most closely associated with variations in mosquito composition. Ae. aegypti and Ae. albopictus were present in both natural and artificial breeding sites and were observed under significantly varying conditions of pH, salinity, dissolved oxygen, and temperature. For Ae. albopictus, larval habitat type and pH were the best predictors of incidence and abundance. For Ae. aegypti, pH and salinity were the best predictors of abundance, while dissolved oxygen and larval habitat type were better predictors of presence. This information broadens our understanding of the ecology and interaction of the investigated species with abiotic factors in the aquatic environments, providing useful data for studies that seek to elucidate the underlying mechanisms of selection and colonization of breeding sites by these mosquitoes. This study also reinforces previous observations indicating that Ae. albopictus and Ae. aegypti can colonize diverse types of larval habitats with widely varying physical and chemical conditions.
Subject(s)
Aedes/physiology , Parks, Recreational , Water/chemistry , Animals , Brazil/epidemiology , Cities , Ecosystem , Larva/growth & development , TemperatureABSTRACT
Two waste fired brick powders coming from brick factories located in Argentine and Czech Republic were examined as alternative mineral admixtures for the production of blended cements. In pastes composition, local Portland cements (Argentine and Czech) were substituted with 8-40%, by mass, with powdered ceramic waste. For the ceramic waste-Portland cement system, workability, the heat released, pozzolanity, specific density, compressive strength, hydrated phases, porosity, and pore size distribution were tested. The relevance of the dilution effect, filler effect, and pozzolanic activity was analyzed to describe the general behavior of the pozzolan/cement system. The properties and performance of cement blends made with finely ground brick powder depended on the composition of ceramic waste and its reactivity, the plain cement used, and the replacement level. Results showed that the initial mini-slump was not affected by a low ceramic waste replacement (8% and 16%), and then it was decreased with an increase in the ceramic waste content. Brick powder behaved as a filler at early ages, but when the hydration proceeded, its pozzolanic activity consumed partially the calcium hydroxide and promoted the formation of hydrated calcium aluminates depending on the age and present carbonates. Finally, blended cements with fired brick powder had low compressive strength at early ages but comparable strength-class at later age.
ABSTRACT
The objective of the present study was to investigate the responses of the chironomid communities (Diptera: Chironomidae) to environmental variables in four moderately and highly disturbed rivers located in one of the most degraded watersheds in South America. Sampling campaigns were carried out during 2014-2016 in four sites of the Matanza-Riachuelo basin. The physical-chemical and hydrological variables were measured and, the ecological indices were calculated and evaluated by ANOVA. The responses of Chironomidae to the environmental variables were evaluated by redundancy analysis (RDA), and the sampling sites were grouped according to the populations of chironomids and the main environmental variables. Finally, the Spearman correlation was made to determine which of these variables were significant. In total, 13 chironomid taxa were found in 36 samples during the study period. The greatest density registered belongs to Rheotanytarsus and Cricotopus. The ANOVA detected the greatest Chironomidae density and taxonomic richness in the sites with agricultural-urban impact. The changes in the distribution of Rheotanytarsus, Thienemanniella, and Polypedilum were mainly explained by the increase in current velocity, organic matter, and hardness, and the decrease of NH3 and BOD. On the other hand, Goeldichironomus, Chironomus, Parachironomus, Dicrotendipes, and Cricotopus were explained by the increase in conductivity, dissolved oxygen, and temperature, and the decrease of the variables NO3, BOD, and Cu. In addition to this, the sites with urban-agricultural impact were clearly separated from sites with urban-industrial impact. The last one was more related to the increase in BOD, Cu, and NO3 that indicates moderate to poor water quality. In conclusion, we can infer that the physical and chemical variables are correlated with changes in the structure and distribution of the chironomid community and there are genera that respond differently at high and intermediate situations of disturbances. This knowledge contributes to the execution of strategies for the conservation and restoration of the lotic ecosystems.
Subject(s)
Chironomidae/growth & development , Environmental Monitoring/methods , Rivers/chemistry , Water Pollution/adverse effects , Water Quality , Animals , Argentina , Biodiversity , Chironomidae/classification , Population Dynamics , South America , Temperature , Water Pollution/analysisABSTRACT
O objetivo deste estudo foi avaliar os rótulos e as características físico-químicas de leite e derivados produzidos em indústrias maranhenses. Para isso, 139 amostras, sendo 48 de leite pasteurizado integral, 48 de iogurte, 26 de queijos, 10 de bebida láctea e 06 de doce de leite, foram submetidas a análises físico-químicas. Os resultados das análises foram confrontados com as respectivas legislações em vigor. Adicionalmente, foram analisados todos os rótulos das amostras, comparando-os ao padrão proposto pela Agência Nacional de Vigilância Sanitária, averiguando possíveis irregularidades. No período avaliado, seis amostras de leite pasteurizado integral, oito de iogurte, quatro de queijo e duas de doce de leite apresentaram parâmetros físico-químicos divergentes das legislações. Dentre os 139 rótulos avaliados identificaram-se 38,13% de inadequações das informações obrigatórias. As principais irregularidades encontradas foram: não possui identificação do lote visível, legível e indelével; ausência de porção alimentar em g ou mL e medida caseira correspondente; não apresenta prazo de validade de forma clara e precisa; ausência de identificação da origem completa discriminada no rótulo; ausência de orientação quanto à correta conservação de alimentos perecíveis a ocorrência de vocábulos e/ou imagens induzindo ao equívoco do consumidor. Desta forma, conclui-se que a qualidade físico-química das amostras estava na sua maioria adequada aos padrões físico-químicos vigentes, entretanto, as irregularidades observadas nos rótulos podem induzir o consumidor ao consumo incorreto desses alimentos
The objective of the study was to evaluate the labels and physical and chemical characteristics of milk and milk products produced in industries of the state of Maranhão, Brazil. For this, 139 samples, 48 of pasteurized whole milk, 48 of yoghurt, 26 of cheeses, 10 of milk and 6 of of sweet paste milk, were submitted to physical and chemical analysis. The results of the analyzes were compared with the respective legislation in force. In addition, all the labels of the samples were analyzed, comparing them to the standard proposed by the National Agency of Sanitary Surveillance, investigating possible irregularities. In the period evaluated, six samples of pasteurized whole milk, eight of yoghurt, four of cheese and two of sweet paste milk presented physical and chemical parameters divergent from the legislations. Among the 139 labels evaluated, 38.13% of the inadequacies of the mandatory information were identified. The main irregularities were: no visible, readable and indelible lot identification; absence of food portion in g or mL and corresponding home measure; without a clear and precise period of validity; absence of identification of the complete origin broken down on the label; absence of guidance on the correct conservation of perishable foods the occurrence of words and/or images leading to consumer misconception. Thus, it was concluded that the physical and chemical quality of the samples was in accordance with the current physical and chemical standards. However, the irregularities observed in the labels can induce the consumer to misuse these foods
ABSTRACT
O objetivo deste estudo foi avaliar os rótulos e as características físico-químicas de leite e derivados produzidos em indústrias maranhenses. Para isso, 139 amostras, sendo 48 de leite pasteurizado integral, 48 de iogurte, 26 de queijos, 10 de bebida láctea e 06 de doce de leite, foram submetidas a análises físico-químicas. Os resultados das análises foram confrontados com as respectivas legislações em vigor. Adicionalmente, foram analisados todos os rótulos das amostras, comparando-os ao padrão proposto pela Agência Nacional de Vigilância Sanitária, averiguando possíveis irregularidades. No período avaliado, seis amostras de leite pasteurizado integral, oito de iogurte, quatro de queijo e duas de doce de leite apresentaram parâmetros físico-químicos divergentes das legislações. Dentre os 139 rótulos avaliados identificaram-se 38,13% de inadequações das informações obrigatórias. As principais irregularidades encontradas foram: não possui identificação do lote visível, legível e indelével; ausência de porção alimentar em g ou mL e medida caseira correspondente; não apresenta prazo de validade de forma clara e precisa; ausência de identificação da origem completa discriminada no rótulo; ausência de orientação quanto à correta conservação de alimentos perecíveis a ocorrência de vocábulos e/ou imagens induzindo ao equívoco do consumidor. Desta forma, conclui-se que a qualidade físico-química das amostras estava na sua maioria adequada aos padrões físico-químicos vigentes, entretanto, as irregularidades observadas nos rótulos podem induzir o consumidor ao consumo incorreto desses alimentos.
The objective of the study was to evaluate the labels and physical and chemical characteristics of milk and milk products produced in industries of the state of Maranhão, Brazil. For this, 139 samples, 48 of pasteurized whole milk, 48 of yoghurt, 26 of cheeses, 10 of milk and 6 of of sweet paste milk, were submitted to physical and chemical analysis. The results of the analyzes were compared with the respective legislation in force. In addition, all the labels of the samples were analyzed, comparing them to the standard proposed by the National Agency of Sanitary Surveillance, investigating possible irregularities. In the period evaluated, six samples of pasteurized whole milk, eight of yoghurt, four of cheese and two of sweet paste milk presented physical and chemical parameters divergent from the legislations. Among the 139 labels evaluated, 38.13% of the inadequacies of the mandatory information were identified. The main irregularities were: no visible, readable and indelible lot identification; absence of food portion in g or mL and corresponding home measure; without a clear and precise period of validity; absence of identification of the complete origin broken down on the label; absence of guidance on the correct conservation of perishable foods the occurrence of words and/or images leading to consumer misconception. Thus, it was concluded that the physical and chemical quality of the samples was in accordance with the current physical and chemical standards. However, the irregularities observed in the labels can induce the consumer to misuse these foods.
Subject(s)
Chemical Phenomena , Dairy Products/analysis , Food Labeling/legislation & jurisprudence , Food IndustryABSTRACT
O objetivo deste estudo foi avaliar os rótulos e as características físico-químicas de leite e derivados produzidos em indústrias maranhenses. Para isso, 139 amostras, sendo 48 de leite pasteurizado integral, 48 de iogurte, 26 de queijos, 10 de bebida láctea e 06 de doce de leite, foram submetidas a análises físico-químicas. Os resultados das análises foram confrontados com as respectivas legislações em vigor. Adicionalmente, foram analisados todos os rótulos das amostras, comparando-os ao padrão proposto pela Agência Nacional de Vigilância Sanitária, averiguando possíveis irregularidades. No período avaliado, seis amostras de leite pasteurizado integral, oito de iogurte, quatro de queijo e duas de doce de leite apresentaram parâmetros físico-químicos divergentes das legislações. Dentre os 139 rótulos avaliados identificaram-se 38,13% de inadequações das informações obrigatórias. As principais irregularidades encontradas foram: não possui identificação do lote visível, legível e indelével; ausência de porção alimentar em g ou mL e medida caseira correspondente; não apresenta prazo de validade de forma clara e precisa; ausência de identificação da origem completa discriminada no rótulo; ausência de orientação quanto à correta conservação de alimentos perecíveis a ocorrência de vocábulos e/ou imagens induzindo ao equívoco do consumidor. Desta forma, conclui-se que a qualidade físico-química das amostras estava na sua maioria adequada aos padrões físico-químicos vigentes, entretanto, as irregularidades observadas nos rótulos podem induzir o consumidor ao consumo incorreto desses alimentos.(AU)
The objective of the study was to evaluate the labels and physical and chemical characteristics of milk and milk products produced in industries of the state of Maranhão, Brazil. For this, 139 samples, 48 of pasteurized whole milk, 48 of yoghurt, 26 of cheeses, 10 of milk and 6 of of sweet paste milk, were submitted to physical and chemical analysis. The results of the analyzes were compared with the respective legislation in force. In addition, all the labels of the samples were analyzed, comparing them to the standard proposed by the National Agency of Sanitary Surveillance, investigating possible irregularities. In the period evaluated, six samples of pasteurized whole milk, eight of yoghurt, four of cheese and two of sweet paste milk presented physical and chemical parameters divergent from the legislations. Among the 139 labels evaluated, 38.13% of the inadequacies of the mandatory information were identified. The main irregularities were: no visible, readable and indelible lot identification; absence of food portion in g or mL and corresponding home measure; without a clear and precise period of validity; absence of identification of the complete origin broken down on the label; absence of guidance on the correct conservation of perishable foods the occurrence of words and/or images leading to consumer misconception. Thus, it was concluded that the physical and chemical quality of the samples was in accordance with the current physical and chemical standards. However, the irregularities observed in the labels can induce the consumer to misuse these foods.(AU)
Subject(s)
Food Labeling/legislation & jurisprudence , Chemical Phenomena , Dairy Products/analysis , Food IndustryABSTRACT
The objective of this work was to evaluate the microbiological, physical and chemical qualities of frozen fruit pulp of different flavors and brands, marketed in the city of Cuiabá - MT. The experiment was conducted in DIC, arranged by a 3 x 2 factorial scheme, using samples from three brands (X, Y and Z) and from two sample collecting periods (June/2014 and October/2014) and in triplicate. The following analyses were performed: coliforms at 35 oC and 45 45 oC, Salmonella, yeasts and molds, color (L*a*b*), pH, titratable acidity, soluble solids, ratio and vitamin C. All of the analyzed frozen fruit pulp samples, regardless of the flavor, the brand and the sampling period, none of them showed coliforms at 35 oC and 45 oC, and no Salmonella either; and low counts of filamentous fungi and yeasts were found. Variations on the color (L*a*b*), pH, titratable acidity, soluble solids, ratio and vitamin C values were found among the brands and sampling periods of all of the analyzed fruit pulps samples. Some samples showed values which did not follow the limits prescribed by the NI nº 1 of 7 January 2000 of the MAPA, indicating unsatisfactory identity and quality standards.(AU)
Neste trabalho foram avaliadas as qualidades microbiológica, física e química de polpas de frutas congeladas de diferentes sabores e marcas, comercializadas no município de Cuiabá MT. O experimento foi conduzido em DIC, disposto em um arranjo fatorial 3 x 2, em amostras de três marcas (X, Y e Z) de dois períodos de coletas (junho/2014 e outubro/2014), e em triplicata. Foram realizadas análises de: coliformes a 35 oC e a 45 oC, Salmonella spp., fungos filamentosos e leveduras, cor (L*a*b*), pH, acidez titulável, sólidos solúveis, ratio e vitamina C. Todas as amostras de polpas de frutas congeladas analisadas, independentemente do sabor, da marca e do período de coleta, não apresentaram contagens de coliformes a 35 oC e a 45 oC, tampouco houve isolamento de Salmonella spp.; e baixas contagens de fungos filamentosos e leveduras foram detectadas. Houve variações dos valores de cor (L*a*b*), pH, acidez titulável, sólidos solúveis, ratio e vitamina C entre as marcas e os períodos de coletas em todas as amostras de polpas de frutas analisadas. Algumas amostras de polpas demonstraram valores fora dos limites preconizados pela IN nº 1 de 7 de janeiro de 2000 do MAPA, que indicaram padrões de identidade e de qualidade deficientes.(AU)
Subject(s)
Fruit/chemistry , Frozen Foods , Reference Standards , Microbiota , Food Quality , Chemical PhenomenaABSTRACT
The present study was carried out to evaluate the phytoplankton community and water quality in four parallel fishponds (FP1 to FP4) during one year. Some abiotic parameters of water column and phytoplankton was sampled in four sites, one in each fishpond. Similarity analysis showed that FP2 fishpond was markedly different for the others ponds with regard to phytoplankton community. Conductivity, orthophosphate, temperature, TSS and hardness parameters were significantly different (p<0.05) between fishponds and all limnological parameters were significantly different (p<0.01) among period (months). Organic matter and phosphorus of the sediment were significantly different (p<0.01) only among fishponds. In current study Chlorophyceae and Bacillariophyceae occurred in most months. Cyanobacteria density in April and May was higher in all fishponds, at FP3 represented 71.4% in April and FP1 represented 78.7% in May of total reported, with chlorophyll-a content lower than 41 mg.L−1. The physical and chemical parameters of fishponds water constituted relations between the parameters and algae occurrence. The present study however, indicated that Cyanobacteria was more frequent in the fishponds after the fish grow-out period (April), and the water quality should be monitored more frequently in this period, due to the high total phosphorus, orthophosphate and chlorophyll-a contents.
O presente estudo avaliou durante um ano a comunidade fitoplanctônica e os fatores abióticos da água de quarto tanques de piscicultura (FP1 ao FP4) com disposição paralela. Alguns fatores abióticos da água e do fitoplâncton foram amostrados em quarto pontos, localizados um em cada tanque de piscicultura. Análise de similaridade mostrou que o tanque de piscicultura FP2 foi diferente dos outros tanques em relação a comunidade fitoplanctônica. Condutividade, ortofosfato, temperatura, STS e dureza foram diferentes (p<0,05) entre tanques e todas as variáveis limnológicas variaram entre o período de estudo (p<0,01). Matéria orgânica e fósforo do sedimento variaram (p<0,01) somente entre os tanques. Chlorophyceae e Bacillariophycea estiveram presentes em todos os meses amostrados. Em abril e maio foi observada elevada densidade de Cyanobacteria em todos os tanques sendo que no FP3 representou 71,4% em abril e no FP1 78,7% em maio do total encontrado, com teor de clorofila-a abaixo de 41 mg.L−1. Os parâmetros físico-químicos da água dos tanques de piscicultura apresentaram relação com a ocorrência do fitoplâncton nestes sistemas. Os resultados deste estudo indicaram a maior frequência de Cyanobacteria no período após a engorda de peixes (abril) necessitando maior monitoramento da qualidade da água devido aos altos teores de fósforo total, ortofosfato e clorofila-a observados neste mês (abril).
Subject(s)
Aquaculture , Fresh Water/analysis , Phytoplankton/classification , Biomass , Chlorophyll/analysis , Geologic Sediments/analysis , Phosphorus/analysis , SeasonsABSTRACT
The present study was carried out to evaluate the phytoplankton community and water quality in four parallel fishponds (FP1 to FP4) during one year. Some abiotic parameters of water column and phytoplankton was sampled in four sites, one in each fishpond. Similarity analysis showed that FP2 fishpond was markedly different for the others ponds with regard to phytoplankton community. Conductivity, orthophosphate, temperature, TSS and hardness parameters were significantly different (p<0.05) between fishponds and all limnological parameters were significantly different (p<0.01) among period (months). Organic matter and phosphorus of the sediment were significantly different (p<0.01) only among fishponds. In current study Chlorophyceae and Bacillariophyceae occurred in most months. Cyanobacteria density in April and May was higher in all fishponds, at FP3 represented 71.4% in April and FP1 represented 78.7% in May of total reported, with chlorophyll-a content lower than 41 mg.L−1. The physical and chemical parameters of fishponds water constituted relations between the parameters and algae occurrence. The present study however, indicated that Cyanobacteria was more frequent in the fishponds after the fish grow-out period (April), and the water quality should be monitored more frequently in this period, due to the high total phosphorus, orthophosphate and chlorophyll-a contents.(AU)
O presente estudo avaliou durante um ano a comunidade fitoplanctônica e os fatores abióticos da água de quarto tanques de piscicultura (FP1 ao FP4) com disposição paralela. Alguns fatores abióticos da água e do fitoplâncton foram amostrados em quarto pontos, localizados um em cada tanque de piscicultura. Análise de similaridade mostrou que o tanque de piscicultura FP2 foi diferente dos outros tanques em relação a comunidade fitoplanctônica. Condutividade, ortofosfato, temperatura, STS e dureza foram diferentes (p<0,05) entre tanques e todas as variáveis limnológicas variaram entre o período de estudo (p<0,01). Matéria orgânica e fósforo do sedimento variaram (p<0,01) somente entre os tanques. Chlorophyceae e Bacillariophycea estiveram presentes em todos os meses amostrados. Em abril e maio foi observada elevada densidade de Cyanobacteria em todos os tanques sendo que no FP3 representou 71,4% em abril e no FP1 78,7% em maio do total encontrado, com teor de clorofila-a abaixo de 41 mg.L−1. Os parâmetros físico-químicos da água dos tanques de piscicultura apresentaram relação com a ocorrência do fitoplâncton nestes sistemas. Os resultados deste estudo indicaram a maior frequência de Cyanobacteria no período após a engorda de peixes (abril) necessitando maior monitoramento da qualidade da água devido aos altos teores de fósforo total, ortofosfato e clorofila-a observados neste mês (abril).(AU)
Subject(s)
Aquaculture , Fresh Water/analysis , /classification , Biomass , Chlorophyll/analysis , Geologic Sediments/analysis , Phosphorus/analysis , SeasonsABSTRACT
As diversas etapas envolvidas na elaboração de quibe cru, quando não realizadas de maneira higiênico-sanitária adequada, podem se tornar potencial disseminador de micro-organismos patogênicos e deteriorantes, podendo alterar seus parâmetros físico-químicos. De acordo com o exposto o objetivo deste estudo foi verificar a qualidade microbiológica e físico-química de quibes crus comercializados em São José do Rio Preto, SP. Nas amostras analisadas, os resultados para clostrídios sulfito redutores, coliformes termotolerantes e Salmonella spp. apresentaram conformidade com a legislação, entretanto, para Staphylococus aureus (coagulase positiva) demonstraram valores superiores ao estabelecido (80,00%), sendo dessa forma recomendada a aplicação de treinamento aos manipuladores, bem como o emprego de Boas Práticas de Fabricação (BPF) e ainda, a higienização adequada de equipamentos e utensílios.(AU)
Several stages involved in the preparation of raw kibbe, when not carried out on a proper sanitary- hygienic manner, can become a potential spreading of pathogenic and deteriorates micro-organisms and can change its physical-chemical parameters. According to the above the objective of this study was to assess the microbiological and physical-chemical quality of raw quibes traded in São José do Rio Preto - SP. In the analysed samples, the results for Clostridiums sulphite reducers, thermo tolerant coliforms and Salmonella spp, submitted accordance with the legislation, however, for Staphylococcus Aureus showed values in excess of established (80.00%), and thus recommended the implementation of training for the manipulators, as well as the employment of Good Manufacturing Practices (GMP) also proper hygienic cleaning of equipment and utensils. (AU)
Subject(s)
Meat Products/microbiology , Food Analysis , Food Microbiology , Consumer Product Safety , BrazilABSTRACT
A instabilidade na qualidade culinária da mandioca é bem conhecida, levando prejuízo na demanda do produto. Neste trabalho, objetivou-se avaliar a interferência da produtividade, precipitação pluviométrica e as características físico-químicas sobre o tempo de cozimento da cultivar IAC 576-70, desde o 6º até o 12º mês após o plantio. Os parâmetros físicos avaliados foram: dificuldade de retirada da entrecasca, dificuldade de cortar em forma de palitos em cortador manual, sendo esses de forma subjetiva. Nas avaliações referentes ao cozimento das raízes foram analisados: a porcentagem de água absorvida pelos toletes, cor dos toletes cozidos, formações de pontuações brancas no interior do tolete, formação de gel em volta dos toletes e tempo de cozimento. Também foram avaliados mensalmente nas raízes: pH, acidez, umidade, cinzas, fibras, extrato etéreo, proteína bruta, açúcares redutores e amido. Concluiu-se que a mandioca de mesa IAC 576-70 quando feito o plantio em julho, na região de Botucatu/SP, deve ser colhida com 9 meses de idade sem prejuízo de produtividade, teor de amido e cozimento das raízes, podendo se estender até 10 meses. O produtor deve acompanhar o somatório do índice pluviométrico nos 10 dias que antecedem a colheita, sendo que este somatório deve ser o menor possível e não proceder a colheita quando este ultrapassar 100 mm, para não comprometer o cozimento das raízes.
The instability of cassava culinary quality is a problem in the market. This work had the purpose of evaluating the interference of the productivity, rain precipitation and physical-chemical characteristics on the cooking time of the IAC 576-70 cultivar, from the 6th to the 12th month after the planting. The physical parameters evaluated were: difficulty in peeling (easy, medium, and hard), difficulty in cutting in long, thin sticks with a manual machine, being those cut in a subjective way. In the analysis of the cooked root, the percentage of water absorbed into the cassava pieces, the color, white points formed inside the pieces of cassava, gel formation around the pieces of cassava, and cooking time were evaluated. The pH, acidity, moisture, ashes, fibers, ether extract, protein, reducing sugars, and starch of the roots were also monthly evaluated. From the results obtained in the present work, it may be concluded that the cassava IAC 576-70, when planted in July, in Botucatu-SP area, must be harvested at the age of nine months, without damage to the productivity, starch level and root cooking, and the harvest could be extended up to ten months. The producers should follow the sum of precipitation index ten days before the harvest, and this value should be the smallest as it may be and the producers should not harvest when this value is more than 100 mm, in order not to hinder the cooking of the root.
ABSTRACT
The present study evaluated the physical and chemical characteristics of the water of the rivers Solimões, Purus and their tributaries, collected in November of 2004 in the State of Amazonas between the cities of Manacapuru and Alvarães and Anamã and Pirarauara. Physical-chemical analyses (temperature, pH, electrical conductivity, turbidity, Ca2+, Na+, K+, Mg2+, HCO3-, SO4(2-), Cl-), and trace-elements (Li, B, Al, Sc, V, Cr, Mn, Fe, Co, Cu, Zn, As, Se, Rb, Sr, Mo, Cd, Sb, Cs, Ba, Pb, La, Ce e U) and Sr isotopes were accomplished. The analyzed parameters and the chemical composition show that the waters of the rivers and tributaries of the central region of the Amazonia are chemically distinct between them. The white waters of Solimões are calcium-bicarbonate and of Purus are bicarbonates, and the respectives tributaries are sodium-potassium-bicarbonate and sodium-potassium-sulphate. This causes the white poorly acided waters to the neutrals and more conductives, while the black ones are less mineralized, more acids, mainly of Purus. The Ba, Sr, Cu, V e As in highest levels differentiate the Solimões white waters of the Purus`s waters, well as the tributaries of the first related to the second. This complex of caracteristics indicates that the Solimões as the Purus and their respectives tributaries are submitted to geological/ambiental distinct conditions. The influence of arrives in port of sediments of Andes is diluted at the currency of basin of the Solimões and it reflects on formation of fertile valleys Solimões and Purus. By another view, the crust rocks, represented by the Shields of the Guianas and Brasileiro both contribute, but in a reduced proportion.
Este trabalho discute as características físico-químicas das águas dos rios Solimões, Purus e seus afluentes, coletadas em novembro de 2004 no Estado do Amazonas, entre as cidades de Manacapuru-Alvarães e Anamã-Pirarauara. Foram realizadas análises físico-químicas (temperatura, pH, condutividade elétrica, turbidez, Ca2+, Na+, K+, Mg2+, HCO3-, SO4(2-), Cl-), de elementos-traço (Li, B, Al, Sc, V, Cr, Mn, Fe, Co, Cu, Zn, As, Se, Rb, Sr, Mo, Cd, Sb, Cs, Ba, Pb, La, Ce e U) e isótopos de estrôncio. Os parâmetros analisados e a composição química mostram que as águas dos rios e igarapés da região central da Amazônia são quimicamente distintas entre si. As águas brancas do Solimões são cálcicas-bicarbonatadas e as do Purus bicarbonatadas, os respectivos afluentes são sódico-potássico-bicarbonatados e sódico-potássico-sulfatados. Isso acarreta águas brancas fracamente ácidas a neutras e mais condutivas, enquanto as pretas são menos mineralizadas, mais ácidas, especialmente as do Purus. O Ba, Sr, Cu, V e As mais elevados diferenciam as águas brancas do Solimões das do Purus, bem como os afluentes do primeiro em relação ao segundo. Esse conjunto de características indicam que tanto o Solimões, como o Purus e os respectivos afluentes, estão submetidos a condições geológicas/ambientais distintas. A influência do aporte de sedimentos dos Andes é diluída ao longo da bacia do Solimões e se reflete na formação das várzeas dos Solimões e Purus. Por outro lado as rochas crustais, representadas pelos escudos das Guianas e Brasileiro também contribuem, mas em menor proporção.
ABSTRACT
The present study evaluated the physical and chemical characteristics of the water of the rivers Solimões, Purus and their tributaries, collected in November of 2004 in the State of Amazonas between the cities of Manacapuru and Alvarães and Anamã and Pirarauara. Physical-chemical analyses (temperature, pH, electrical conductivity, turbidity, Ca2+, Na+, K+, Mg2+, HCO3-, SO4(2-), Cl-), and trace-elements (Li, B, Al, Sc, V, Cr, Mn, Fe, Co, Cu, Zn, As, Se, Rb, Sr, Mo, Cd, Sb, Cs, Ba, Pb, La, Ce e U) and Sr isotopes were accomplished. The analyzed parameters and the chemical composition show that the waters of the rivers and tributaries of the central region of the Amazonia are chemically distinct between them. The white waters of Solimões are calcium-bicarbonate and of Purus are bicarbonates, and the respectives tributaries are sodium-potassium-bicarbonate and sodium-potassium-sulphate. This causes the white poorly acided waters to the neutrals and more conductives, while the black ones are less mineralized, more acids, mainly of Purus. The Ba, Sr, Cu, V e As in highest levels differentiate the Solimões white waters of the Purus`s waters, well as the tributaries of the first related to the second. This complex of caracteristics indicates that the Solimões as the Purus and their respectives tributaries are submitted to geological/ambiental distinct conditions. The influence of arrives in port of sediments of Andes is diluted at the currency of basin of the Solimões and it reflects on formation of fertile valleys Solimões and Purus. By another view, the crust rocks, represented by the Shields of the Guianas and Brasileiro both contribute, but in a reduced proportion.
Este trabalho discute as características físico-químicas das águas dos rios Solimões, Purus e seus afluentes, coletadas em novembro de 2004 no Estado do Amazonas, entre as cidades de Manacapuru-Alvarães e Anamã-Pirarauara. Foram realizadas análises físico-químicas (temperatura, pH, condutividade elétrica, turbidez, Ca2+, Na+, K+, Mg2+, HCO3-, SO4(2-), Cl-), de elementos-traço (Li, B, Al, Sc, V, Cr, Mn, Fe, Co, Cu, Zn, As, Se, Rb, Sr, Mo, Cd, Sb, Cs, Ba, Pb, La, Ce e U) e isótopos de estrôncio. Os parâmetros analisados e a composição química mostram que as águas dos rios e igarapés da região central da Amazônia são quimicamente distintas entre si. As águas brancas do Solimões são cálcicas-bicarbonatadas e as do Purus bicarbonatadas, os respectivos afluentes são sódico-potássico-bicarbonatados e sódico-potássico-sulfatados. Isso acarreta águas brancas fracamente ácidas a neutras e mais condutivas, enquanto as pretas são menos mineralizadas, mais ácidas, especialmente as do Purus. O Ba, Sr, Cu, V e As mais elevados diferenciam as águas brancas do Solimões das do Purus, bem como os afluentes do primeiro em relação ao segundo. Esse conjunto de características indicam que tanto o Solimões, como o Purus e os respectivos afluentes, estão submetidos a condições geológicas/ambientais distintas. A influência do aporte de sedimentos dos Andes é diluída ao longo da bacia do Solimões e se reflete na formação das várzeas dos Solimões e Purus. Por outro lado as rochas crustais, representadas pelos escudos das Guianas e Brasileiro também contribuem, mas em menor proporção.
ABSTRACT
Physical and chemical variables of soil and water were measured to determine the effectiveness of a constructed wetland for wastewater treatment. Eight different macrophyte species, namely Eichhornia crassipes, Alternanthera philoxerodos, Heteranthera reniformis, Hydrocotyle umbeliferae, Ludwigia elegan, Ludwigia sericea, Myriophyllum aquaticum and Thypha domingensis, were transplanted. Inlet water and outlet water were the two sampling sites evaluated. There were significant differences (p 0.05) when limnological characteristics between inlet and outlet water from the constructed wetland were compared. In general, dissolved oxygen was over 4 mg L-1, and conductivity was high, above 80 µS cm-1. Chlorophyll-a levels generally tended to decrease at the wetland outlet and were higher during the rainy period (fish growth period). Results show that ammonia, total phosphorus, BOD5, phosphorus and organic matter in the sediment removals in the constructed wetland were higher, indicating that macrophytes played an important role in removing these variables. The use of constructed wetland is a viable technology for the biological treatment in aquaculture and swine wastewater.
Variáveis físico-químicas da água e do solo foram avaliadas a fim de verificar a eficiência de um wetland construído no tratamento de resíduos. Foram escolhidas oito espécies de macrófitas baseadas na capacidade de retenção e disponibilidade no local de estudo. Dentre elas estão: Eichhornia crassipes, Alternanthera philoxerodos, Heteranthera reniformis, Hydrocotyle umbeliferae, Ludwigia elegan, Ludwigia sericea, Myriophyllum aquaticum e Thypha domingensis. Foram amostrados dois pontos um na entrada e outro na saída de água do wetland. Diferenças significativas (p 0,05) foram observadas entre a entrada e saída do wetland em relação às variáveis limnológicas. Em geral, foram observadas concentrações de oxigênio dissolvido acima de 4 mg L-1 e de condutividade, acima de 80 µS cm-1. As concentrações de clorofila-a decresceram ao passar pelo wetland, sendo mais elevadas no período de chuva (período de engorda de peixe). Amônia, fósforo total, DBO5, fósforo e matéria orgânica do sedimento decresceram ao passar pelo wetland construído para indicar a eficiência desse sistema. Assim, essa tecnologia biológica (macrófitas aquáticas) apresenta-se como uma opção adequada para melhoria de efluentes de aquicultura e de suinocultura.
ABSTRACT
Physical and chemical variables of soil and water were measured to determine the effectiveness of a constructed wetland for wastewater treatment. Eight different macrophyte species, namely Eichhornia crassipes, Alternanthera philoxerodos, Heteranthera reniformis, Hydrocotyle umbeliferae, Ludwigia elegan, Ludwigia sericea, Myriophyllum aquaticum and Thypha domingensis, were transplanted. Inlet water and outlet water were the two sampling sites evaluated. There were significant differences (p 0.05) when limnological characteristics between inlet and outlet water from the constructed wetland were compared. In general, dissolved oxygen was over 4 mg L-1, and conductivity was high, above 80 µS cm-1. Chlorophyll-a levels generally tended to decrease at the wetland outlet and were higher during the rainy period (fish growth period). Results show that ammonia, total phosphorus, BOD5, phosphorus and organic matter in the sediment removals in the constructed wetland were higher, indicating that macrophytes played an important role in removing these variables. The use of constructed wetland is a viable technology for the biological treatment in aquaculture and swine wastewater.
Variáveis físico-químicas da água e do solo foram avaliadas a fim de verificar a eficiência de um wetland construído no tratamento de resíduos. Foram escolhidas oito espécies de macrófitas baseadas na capacidade de retenção e disponibilidade no local de estudo. Dentre elas estão: Eichhornia crassipes, Alternanthera philoxerodos, Heteranthera reniformis, Hydrocotyle umbeliferae, Ludwigia elegan, Ludwigia sericea, Myriophyllum aquaticum e Thypha domingensis. Foram amostrados dois pontos um na entrada e outro na saída de água do wetland. Diferenças significativas (p 0,05) foram observadas entre a entrada e saída do wetland em relação às variáveis limnológicas. Em geral, foram observadas concentrações de oxigênio dissolvido acima de 4 mg L-1 e de condutividade, acima de 80 µS cm-1. As concentrações de clorofila-a decresceram ao passar pelo wetland, sendo mais elevadas no período de chuva (período de engorda de peixe). Amônia, fósforo total, DBO5, fósforo e matéria orgânica do sedimento decresceram ao passar pelo wetland construído para indicar a eficiência desse sistema. Assim, essa tecnologia biológica (macrófitas aquáticas) apresenta-se como uma opção adequada para melhoria de efluentes de aquicultura e de suinocultura.
ABSTRACT
O objetivo principal do presente trabalho foi estudar o impacto dos metais dissolvidos e em sedimentos no Córrego do Cintra, localizadona cidade de Botucatu-SP, à jusante do Campus da Unesp-SP, Brasil. Os metais potencialmente tóxicos dissolvidos na água foram detectados na maioria dos locais de coleta. Os Pontos de 1 a 7 superaram o limite máximo permitido pelo CONAMA para o Cd (até 0,280 mg.L-1), Fe (5,0 a 47,79 mg.L-1), Ni (0,027 a 0,378 mg.L-1), Zn (<5,0 mg.L-1), Pb (0,033 a 0,072 mg.L-1) e Cu (0,028 a 3,82 mg.L-1). Detectaram- se também as possíveis fontes geradoras pontuais (efl uentetratado e não tratado) e difusas (agroquímicos e resíduos lixiviadosda Rodovia Marechal Rondon) que comprometem signifi cativamentea qualidade da água...
The objective of the present study was to investigate the impact of metals dissolved and in sediments in the Cintra Stream located in Botucatu city, SP, downstream Unesp Campus SP, Brazil. The potentially toxic metal dissolved in the water were detected in mostof the collection places. Points 1 to 7 surpassed the maximum index allowed by Resolution of CONAMA nº 357/2005, for the Cd (until 0,280 mg. L-1), Fe (5,0 to 47,79 mg. L-1), Ni (0,027 to 0,378mg. L-1), Zn (< 5,0 mg. L-1), Pb (0,033 to 0,072 mg. L-1), Cu (0,028 to 3,82 mg. L-1). It was also detected the possible focal generating sources (effl uent treated and not treated) and diffuse (agrochemistry and lixiviated residues of the Marechal Rondon high-way) that signifi cantly impairs the water quality...
Subject(s)
Humans , Animals , Male , Female , Biological Availability , Metals/toxicity , Water Pollution, Chemical , Metals/analysis , Metals/adverse effects , Sediments/analysis , Water/analysisABSTRACT
The Puraquequara River and its right-bank's tributary, the Água Branca stream, are located in the outskirts of the Manaus urbanized area and their basin is still harbored by the primary rain forest cover. They exhibit 'terra firme' geologic, pedologic and climatological characteristics pertaining to the Amazonian region. Samples for this research were collected in November 1998 (dry season) and April 1999 (rainy season). Water temperature, pH, turbidity, conductivity, alkalinity, hardness, COD, Ca2+, Mg2+, Na+ e K+, SiO2, NO3-, NO2-, PO4(3-), SO4(2-), Cl-, NH4+ and Fe were analysed. The water is acid (3.8 to 4.1) turning into less acid in the dry season. The alkalinity and turbidity are higher in the dry season whereas conductivity, hardness and COD are higher in the rainy season. SiO2 and Cl- are the main anionic elements where the former's contribution is higher in the Puraquequara and lower in the Água Branca while the latter shows inverse contribution. Na+, Fe, and NH4+ are the main cations with their higher contribution in the dry season. NH4+ is the only cation that tends to increase its contribution downstream in the wet season. K+ contents is higher than that of Mg+ and Ca2+ yet Na+ is the highest. The higher than 0.2 mg/L and 0.5 mg/L amounts of NH4+ and NO3- respectively, are traces of water contamination. These characteristics define these waters as very diluted with anions predominating over cations and correlated to the black water of the region.
O rio Puraquequara, e seu afluente direito, o igarapé Água Branca, localizam-se fora da zona urbana de Manaus e estão com a bacia ainda protegida por floresta primária. Têm características geológicas, pedológicas e climatológicas de igarapés naturais de terra-firme da Amazônia Central. Foram coletadas amostras de água nos meses de novembro de 1998 (período seco) e abril de 1999 (período chuvoso) e determinados os parâmetros temperatura, pH, turbidez, condutividade, alcalinidade, dureza, DQO, Ca2+, Mg2+, Na+, K+, SiO2, NO3-, NO2-, PO4(3-), SO4(2-), Cl-, NH4+ e Fe total. As águas apresentaram pH entre 3,8 e 4,1 passando a menos ácidas na estiagem. A alcalinidade e a turbidez são, em geral, mais elevadas na estiagem, enquanto a condutividade, dureza e DQO são maiores no período mais chuvoso. SiO2 e o Cl- foram os ânions mais abundantes, com contribuição maior do primeiro no Puraquequara e menor no Água Branca, enquanto o Cl- tem comportamento oposto. Na+, Fe total e NH4+ são os cátions mais abundantes e predominam, no geral, no período mais seco. O NH4+ é o único cátion que tende a aumentar sua contribuição para jusante do igarapé no período úmido. Os teores de K+ são mais elevados do que os de Mg2+ e este que os de Ca2+, e são todos superados pelo Na+. Os teores da NH4+ e NO3-, acima de 0,2 mg/L e 0,5 mg/L, respectivamente, são indícios de contaminação. Essas características definem as águas da bacia como muito diluídas, com predominância dos ânions sobre os cátions e correlacionáveis as águas de cor preta.