1.
Bio Protoc
; 8(3): e2719, 2018 Feb 05.
Article
in English
| MEDLINE
| ID: mdl-34179257
ABSTRACT
This protocol delivers a method to determine the biosynthetic capability of comfrey leaves for pyrrolizidine alkaloids independently from other organs like roots or flowers. The protocol applies and combines radioactive tracer experiments with standard and modern techniques like thin layer chromatography (TLC), solid-phase extraction (SPE), high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS).