ABSTRACT
Objective To investigate the effects of different drying methods on composition and content of five active constituents in root bark and root of Polgala tenuifoliaroot. Methods The contents of polygalaxanthone III, 3,6’-disinapoyl sucrose, polygalacic acid, senegenin, and tenuifolin in root bark and root from different drying samples were determined by HPLC. Then the data analysis was performed by ANOVA and TOPSIS methods. Results There is a difference in the order of drying methods for bark and root of P. tenuifoliaroot. For P. tenuifoliaroot root bark, the order of different drying methods was microwave drying > 60 ℃ hot-air drying > 50 ℃ hot-air drying > 70 ℃ hot-air drying > freeze-drying > 40 ℃ hot-air drying > shade drying > sun drying; For P. tenuifolia root, the different drying methods were sorted by microwave drying > 60 ℃ hot-air drying > shade drying > sun drying > 50 ℃ hot-air drying > 40 ℃ hot-air drying > 70 ℃ hot-air drying > freeze-drying. Conclusion Combined with the production practice, this study suggests that microwave drying and hot-air drying at 60 ℃ are suitable drying methods for P. tenuifoliaroot bark and root, providing a basis for the determination of drying methods for the origin processing of P. tenuifolia.
ABSTRACT
Objective To study and establish the HPLC fingerprint of Polgala tenuifolia for the identification and quality controlof P. tenuifolia. Methods Twenty batches of wild and cultivated P. tenuifolia collected from different regions in China were detected by HPLC. The Similarity Evaluation System for Chromatographic Fingerprint of TCM (2012 A edition) was used to evaluate the similarity of the samples. The differences among the samples were identified by chemical pattern recognition methods including principal component analysis (PCA) and partial least squares discriminate analysis (PLS-DA). Results The common model of HPLC fingerprint of wild and cultivated P. tenuifolia was obtained, 24 common peaks were found in the chromatograph. The similarities between wild and cultivated P. tenuifolia fingerprints and control fingerprints in 20 batches from different regions were over 0.86, PCA results demonstrated obvious distinction between the wild and cultivated P. tenuifolia. The wild and cultivated P. tenuifolia was completely distinguished by PLS-DA. Twelve constituents, such as polygalactoneone III and 3,6’-disinapoyl sucrose were screened as biomarkers, representing the major differences between the two varieties. Conclusion The HPLC fingerprint combined with chemical pattern recognition can be used as an effective method for the quality control and identification of the different sources of P. tenuifolia, and provide a reference for the quality control and stoichiometric taxonomy of P. tenuifolia.
