Metabolic profiles of Fufang Xiling Jiedu capsule in rats by ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry.

Fufang Xiling Jiedu capsule (FXJC), a traditional Chinese medicine that evolved from "Yinqiao Powder", is widely used for the treatment of cold and influenza. However, due to a lack of in vivo metabolism research, the chemical components responsible for the therapeutic effects still remain unclear. Hence, this study aimed to describe the metabolic profiles of the FXJC in rat plasma, urine, and feces. A combined data mining strategy based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry was employed and 201 xenobiotics, including 117 prototype components and 84 metabolites were detected. Phenolic acids, flavonoids, triterpenes, and lignans were prominent ingredients absorbed in vivo, and the major metabolic pathways of the detected metabolites were glucuronidation, sulfation, methylation, and oxidation. This is the first systematic study on the metabolism of the FXJC in vivo, providing valuable information for future studies on the efficacy, toxicity, and mechanism of the FXJC.

Analysis of the active components and metabolites of Taohong Siwu decoction by using ultra high performance liquid chromatography quadrupole time-of-flight mass spectrometry.

Taohong Siwu Decoction is a classic Chinese medicine prescription for treatment of cerebral ischemia and gynecological diseases. However, the active ingredients of Taohong Siwu Decoction have not been identified. In this study, a ultra performance liquid chromatography quadrupole time-of-flight mass system was used to analyze the active components and metabolites of Taohong Siwu Decoction absorbed into the blood and the brain. A total of 39 active compounds and 90 metabolites were identified in the blood and brain by comparing retention times, accurate masses, fragmentation patterns, and literature data. The results showed that flavonoids (Carthamus tinctorius L), aromatic organic acids, and benzoquinones (Angelica sinensis (Oliv.) Diels and Ligusticumchuanxiong hort) were prominent active ingredients in Taohong Siwu Decoction. Furthermore, hydrolysis, glucuronidation, and sulfation were identified as the main metabolic pathways of Taohong Siwu Decoction in vivo. This was the first study to characterize the active components and metabolites of Taohong Siwu Decoction in the blood and brain using ultra performance liquid chromatography quadrupole time-of-flight mass.

Identification of absorbed components and their metabolites in rat plasma after oral administration of Liujing Toutong Tablets by HPLC-Q/TOF-MS / 中草药

Objective To study the serum pharmacochemistry of Liujing Toutong Tablets (LTT). This study could be helpful to elucidate the bioactive constituents of LTT. Methods HPLC-Q/TOF-MS was used to analyze the ingredients in rats blood plasma with LTT (ig). Results By comparing the information on the total ion chromatogram, extraction chromatogram and the mass spectrogram of LTT, rat serum with drug and blank serum sample was used to confirm the constituents of LTT in vivo. As a result, a total of 46 compounds were detected in rat plasma, including 24 absorbed prototype constituents, such as puerarin, and 22 of the metabolites. Conclusion The compounds are absorbed into the blood might function as real bioactive constituents and this study can provide a scientific fundament for bioactive substances of LTT.

Rapid Analysis and Identification of Absorbed Components and Their Metabolites of Yuanhu Zhitong Dropping Pill in Rat Plasma and Brain Tissue Using UPLC-Q-TOF/MS with Multivariate Statistical Analysis / 中草药·英文版

Objective: To establish an effective approach for rapid and comprehensive analysis on the absorbed and metabolic components in rats after ig administration of Yuanhu Zhitong Dropping Pill (YHZT). Methods: Based on the combination of UPLC-Q-TOF/MS and multivariate statistical analysis, the absorbed prototype constituents and their metabolites in rat plasma were rapidly analyzed and identified, and the components absorbed into brain were further identified by comparing the extracted ion chromatograms (EICs) of control and brain tissue samples of dosed rats. Results: A total of 38 YHZT-related xenobiotic compounds were detected and identified as the potential bioactive constituents in rat plasma, including 24 absorbed prototype constituents and 14 metabolites. In particular, of all prototype constituents, 14 were also detected in rat brain tissue, indicating that they could penetrate the blood-brain barrier and enter into brain. Conclusion: An effective method is established and applied to analyze the potential bioactive constituents in YHZT, which provides a pathway to further investigate the pharmacological pattern and mechanism of YHZT.

RRLC-TOF/MS in identification of plasma constituents and metabolites of epimedium in rats after intragastric administration / 第二军医大学学报

Objective To identify the constituents and metabolites of epimedium in rat plasma by rapid-resolution liquid chromatography-time of flight mass spectrometry (RRLC-TOF/MS) after intragastric administration, so as to explore the active ingredients and metabolites of epimedium in vivo. Methods The separation was performed on a SHISEIDO MG C18 reverse phase column (3. 0 mm×100 mm, 3 μm). The mobile phase consisted of water containing 0. 1% formic acid and acetonitrile was used as gradient elute. The flow ratewas 0. 6 mL/min, post-column split ratio was 3) 1, and the temperature of column was 25°C. Time-of-flight mass spectrometer(TOF/MS) and electrospray ion source (ESI) were applied for qualitative analysis under positive ion mode, with the mass scan range being m/z 100-1 000. Results Three prototype constituents and 4 metabolites of epimedium were identified in rat plasma. Conclusion A rapid and efficient RRLC-TOF/MS method has been established for studying the constituentsand metabolites of epimedium in vivo, which provides a reference for further pharmacodynamics experiments.