Identification and determination of chemical constituents from Yinchen Qingjin granules by ultra high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry.

An ultra high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry method had been used to identify chemical constituents in Yinchen Qingjin Granules. A total of 117 chemical constituents were tentatively identified based on the molecular weight, the mass fragmentation behavior, and the comparison with retention time and accurate mass of reference standards. Meanwhile, an ultra high-performance liquid chromatography coupled with triple quadrupole mass spectrometry method has been developed to evaluate the quality of Yinchen Qingjin Granules through a simultaneous determination of ten components, namely gallic acid, shanzhiside, neochlorogenic acid, chlorogenic acid, caffeic acid, geniposide, liquirition, isoliquirition, glycyrrhizic acid, and rhein. Altogether, content levels of each compound ranged from 0.228 to 8.712 mg/g. At the same time, a hydrophilic interaction liquid chromatography combined with UV for the quantification of sarmentosin in Yinchen Qingjin Granules is presented and its content was from 5.608 to 6.200 mg/g. The linearity, accuracy, and precision of these two validation methods were satisfactory. The both determination methods were successfully used for the analysis of six batches of Yinchen Qingjin Granules samples made in China and could be as suitable analytical method for quality control.

Effects of Qingjin Granules on Methicillin-resistant Staphylococcus Aureus Pneumonia Mice / 中国中医药信息杂志

Objective To observe the effects of Qingjin Granules on methicillin-resistant staphylococcus aureus (MRSA) pneumonia mice. Methods Pneumonia model was prepared by intranasal drip of bacterial fluid. Experimental mice were randomly divided into control group, model group, Western medicine group and TCM group. On the third day after infection, the Western medicine group was treated with linezolid, and the TCM group was given Qingjin Granules for gavage. The control and model group were given the same amount of normal saline for gavage. Body weight and clinical manifestations every day of each mouse after infected were recorded. Lung scans at day 3 and day 10 were taken; the pathological changes trough HE staining were observed; microbial load of lung tissue was detected. Results There were significant weight losses on mice except control group on the first day after infection. CT scan showed that the lung inflammation was filled with mice on the third day after infection. The lung inflammation in the TCM group was more reduced than model group, with less inflammatory exudate and only scattered inflammatory exudates. Meanwhile, pulmonary inflammatory pathology was reduced and the amount of bacteria were reduced in the lungs of TCM group (P<0.05), but it was less effective than linezolid (P<0.05). Conclusion Qingjin Granules can significantly reduce the microbial load of the lung and infiltration of inflammatory cells in lung tissue of mice, achieving the efficacy of treating MRSA pneumonia in mice.