ABSTRACT
OBJECTIVE:To study whether Radix Inulae can replace Radix Aristolochiae as medicinal material.METHO_ DS:Comparison was made between Radix Inulae and Radix Aristolochiae in terms of plant resources,functions and indications,chemical compositions,pharmacologic actions,clinical applications,etc.by reviewing literature.RESULTS&CONCLUSIONS:Radix Inulae and Radix Aristolochiae were different in every aspect,thus the two can’t be replaced by each other as medicinal material.
ABSTRACT
Objective To study the pharmacokinetics of the active component in series of Guanxin Suhe Pills including the prescription containing Radix Aristolochiae(QG),the prescription containing Radix Inulae(TG),and the prescription without any of them(BG) in Beagle dogs and to compare the pharmacokinetics among the series of prescription.Methods HPLC Method was developed to determine acid in plasma of Beagle dogs.Blood plasma was collected after series of Guanxin Suhe Pills were ig administrated to Beagle dogs 0—8 h later.The concentration of cinnamic acid in the plasma was determined and DAS 2.1 software was used in calculation of compartment model and pharmacokinetic parameters.Results The standard curve was linear from 0.031 25 to 32 mg/L in plasma of Beagle dogs,the lowest detectable limit was 31.25 ?g/L,the recovery rate of the method was over 90 % with satisfactory relative standard deviations(RSDs) of intra-day and inter-day both lower than 5 %.The metabolism of cinnamic acid in plasma of Beagle dogs after medication of series in QG,TG,and BG all fitted in a first order absorption of two-compartment model.The peak concentration and the area under curve in TG and BG groups had the higher tendency than those in the QG group,but there was no significant difference among the three groups.Conclusion The series of Guanxin Suhe Pills all have a similar pharmacokinetic process.Taking away the Radix Aristolochiae from the previous prescription that including the Radix Aristolochiae or taking the place of the Radix Aristolochiae with Radix Inulae does not influence the pharmacokinetic process of the main active component.
ABSTRACT
AIM: To observe the change of Guanxi Suhe Pill(GXSH) in cardiomyocytes suffering from hydrogen peroxide-induced injury due to replacement of Aristolochia debilis sieb.et Zucc by Radix inulae. METHODS: Myocardial cells from neonatal SD rats were cultured in dulbecco's modified eagle's medium(DMEM).The primary culture cardiomyocytes were injured by hydrogen peroxide(1 mmol/L) for 4 h,and serum containing GXSH was added to the medium at the same time.The creatine kinase(CK) activity,lactate dehydrogenase(LDH) activity and malondialdehyde(MDA) content in culture medium were determined.Intracellular superoxide dismutase(SOD) activity,the activity of Na~+-K~+-ATPase and Ca~(2+)-Mg~(2+)-ATPase in the cardiomyocytes were also observed. RESULTS: GXSH containing Aristolochia debilis sieb.et Zucc,GXSH containing Radix inulae,and GXSH without above both herbs significantly inhibited the release of LDH and CK,decreased the content of MDA and increased SOD activity,increased the volume of Na~+-K~+-ATPase and Ca~(2+)-Mg~(2+)-ATPase of hydrogen peroxide-injured cardiomyocytes.The effects of TG and BG were stronger significantly than those of QG on some parameters. CONCLUSION: GXSH all has a remarkable protective effect on cardiomyocytes suffering from hydrogen peroxide-induced injury without and with Aristolochia debilis sieb.et Zucc or taking the place of the GXSH containing Radix inulae do not influence its protective effects on cardiomyocytes suffereing from hydrogen peroxide-induced injury.
