ABSTRACT
Objective:To simultaneously determine six components ( paeoniflorin, rosmarinic acid, salvianolic acid B, ligustilide, clyptotanshinone and tanshinone ⅡA ) in refined coronary tablets by UHPLC-DAD. Methods: A Dikma Endevaorsil C18 column (2. 1 mm × 100 mm,1. 8 μm) was used to perform the determination, which was maintained at 30℃ during the analysis. The mobile phase was composed of acetonitrile and 0. 05% phosphoric acid at a flow rate of 0. 3 ml·min-1 with gradient elution. The detection wavelength was respectively set at 230,270,288 and 321 nm. Results:Paeoniflorin, rosmarinic acid, salvianolic acid B, ligustilide, clyptotanshinone and tanshinone ⅡA showed good linearity within the range of 0. 001 0-0. 010 2 μg ( r = 0. 999 8 ), 0. 005 7-0.056 9 μg(r =1.000 0), 0.005 3-0.052 7 μg(r = 1.000 0),0.002 1-0.020 6 μg(r = 1.000 0),0.001 1-0.011 2 μg(r =1. 000 0) and 0. 001 4-0. 014 4 μg(r=0. 999 8),respectively. The average recovery was 98. 78%(RSD=0. 50%), 97. 99%(RSD=0. 76%),98. 44%(RSD=0. 85%),99. 12%(RSD=0. 66%), 98. 82%(RSD=0. 81%) and 97. 80%(RSD=0. 80%), respec-tively. Conclusion:The method is simple, rapid and accurate, which can be used for the quality control of refined coronary tablets.
ABSTRACT
Objective: To develop an HPLC method for the simultaneous determination of tanshinone IIA, paeoniflorin, salvianolic acid B, ferulic acid, safflor yellow A, ligustilide, and danshensu in Refined Coronary Tablets. Methods: The chromatographic separation was achieved on a Zorbax Eclipse XDB-C18 column (250 mm × 4.6 mm, 5.0 μm) with methanol-acetonitrile (25∶75, A)-0.1% formic acid (B) as mobile phases at the flow rate of 1.0 mL/min for gradient elution: 0-10.0 min, 95% B; 10.0-16.0 min, 95%-85% B; 16.0-18.0 min, 85% B; 18.0-22.0 min, 85%-75% B; 22.0-26.0 min, 75%-65% B; 26.0-40.0 min, 65%-15% B; Detection with variable wavelength: 0-19.0 min was 270 nm, 19.0-22.0 min was 230 nm, 22.0-27.0 min was 320 nm, 27.0-40.0 min was 402 nm, and the column temperature was 30 ℃. Its linear relationship, precision, repeatability, stability, and recoveries were investigated. Results: The results showed that the seven active components were well separated and showed good linearity, tanshinone IIA 0.4-8.0 mg/L (r = 0.999 5), paeoniflorin 1.2-24.0 mg/L (r = 0.999 1), salvianolic acid B 3.2-64.0 mg/L (r = 0.999 3), ferulic acid 0.08-1.60 mg/L (r = 0.999 5), safflor yellow A 1.2-24.0 mg/L (r = 0.999 3), ligustilide 0.24-4.80 mg/L (r = 0.999 7), and danshensu 0.32-6.40 mg/L (r = 0.999 7). The precision was good, and RSD was less than 2.0%. The repeatability was good, and RSD was less than 2.0%. The stability was good in 12 h. The average recoveries were between 98.05%-101.27%, and RSD was less than 2.0%. The contents of target components in Refined Coronary Tablets, tanshinone was 0.704-0.797 mg/g, paeoniflorin was 3.124-3.411 mg/g, salvianolic acid B was 7.129-7.611 mg/g, ferulic acid was 0.180-0.198 mg/g, safflor yellow A was 2.718-2.966 mg/g, ligustilide was 0.590-0.683 mg/g, and danshensu was 0.811-0.899 mg/g. Conclusion: The method is accurate, sensitive, credible, and repeatable. It can be applied to the quality control of Refined Coronary Tablets.
ABSTRACT
OBJECTIVE:To establish a HPLC method for content determination of paeoniflorin in refined coronary tablets.METHODS:HPLC was performed on Kromasil C18 column at room temperature,the mobile phase consisted of acetonitrile-0.2%phosphoric acid solution(13∶87),the detection wavelength was 230nm with flow rate 1ml/min and sample size 10?l.RESULTS:Paeoniflorin was linear with the peak area in the range of 0.317?g～1.587?g(r=0.9 999),the average recovery was 100.1%(RSD=1.46%).CONCLUSION:The established method is simple,reliable,reproducible and can be used for the quality control of refined coronary tablets.
