Steam distillation, supercritical fluid extraction, and anti-Trypanosoma cruzi activity of compounds from pink pepper (Schinus terebinthifolius Raddi).

Anti-Trypanosoma cruzi activity of compounds from fruits of Schinus terebinthifolius Raddi (pink pepper) were evaluated, using sustainable techniques such as steam distillation (SD) and supercritical fluid extraction (SFE). SD was optimised using a design of experiment and SFE was carried out using supercritical CO2 solvent (300 bar and 60 °C). Results of the anti-T. cruzi activity showed that the essential oil presented high activity (IC50 = 4.5 ± 0.3 µg/mL), whereas the supercritical extract had a moderate effect (IC50 = 19.7 ± 2.9 µg/mL). The differences in the anti-T. cruzi activity can be attributed to the extraction of non-volatile compounds in the SFE, such as moronic and (Z)-masticadienoic acids. In contrast, SD extracted only volatile compounds such as monoterpenes and sesquiterpenes. Therefore, these results suggest that the volatile compounds from pink pepper are involved with the anti-T. cruzi activity.

Evaluation of the antimicrobial activity of silver nanoparticles biosynthesized from the aqueous extract of Schinus terebinthifolius Raddi leaves.

Silver nanoparticles (AgNPs) synthesized from green synthesis using medicinal plants are presented as an option for the development of new antimicrobial agents. In this context, this study aims to evaluate the antimicrobial activity of silver nanoparticles synthesized from the aqueous extract of Schinus terebinthifolius Raddi leaves, popularly known as "aroeira." A 23 factorial design was used to assess the statistical significance of the studied factors, and Box-Behnken design was used to define the optimal conditions for the green synthesis of AgNPs. The studied factors were significant for the synthesis of nanoparticles, and the optimal conditions were pH 9.5, reaction time of 180 min, and 10 g of S. terebinthifolius Raddi leaves. The samples were characterized using UV-Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and dynamic light scattering. Antimicrobial activity was evaluated using the broth microdilution method and through the minimum microbicidal concentration. AgNPs showed greater antimicrobial efficacy compared to the extract, both for bacteria and fungi: bacteriostatic activity against all tested bacteria and bactericidal against Pseudomonas aeruginosa, besides fungistatic action against Candida albicans and Candida glabrata. Finally, the aqueous extract of "aroeira" leaves was effective in the synthesis of AgNPs, with greater antimicrobial potential than the extract.

Silver nanoparticles obtained from Brazilian pepper extracts with synergistic anti-microbial effect: production, characterization, hydrogel formulation, cell viability, and in vitro efficacy.

The synthesis of silver nanoparticles using plant extracts is known as a green approach, as it does not require the use of high pressure, energy, high temperature, or toxic chemicals. The approach makes use of plant extracts in a process called bioreduction, which is mediated by enzymes, proteins, amino acids, and metabolites found in bark, seed, and leaf extracts, transforming silver ions into metallic silver. This work aimed at developing silver nanoparticles (AgNPs) from Brazilian pepper, applying this green methodology. Hydroalcoholic extract of leaves of Schinus terebinthifolius Raddi was prepared and its concentration of polyphenols, tannins, and saponins quantified. The produced nanoparticles were characterized by UV-Vis spectroscopy, thermogravimetric analysis (TG), dynamic light scattering (DLS), and zeta potential (ZP). AgNPs were formulated in sodium alginate hydrogels to obtain a nano-based semi-solid formulation for skin application. The obtained silver nanoparticles of mean size between 350 and 450 nm showed no cytotoxicity against L929 mouse fibroblasts within the concentration range of 0.025 mg/mL and 10 mg/mL. Schinus terebinthifolius Raddi was found to enhance microbial inhibition against the tested strains, especially against gram-negative bacteria. Its potential use as an alternative to overcome bacterial resistance can be expected.

Effect of aroeira (Schinus terebinthifolius Raddi) fruit against polyunsaturated fatty acids and cholesterol thermo-oxidation in model systems containing sardine oil (Sardinella brasiliensis).

This study evaluated the protective effect of ground aroeira (Schinus terebinthifolius Raddi) fruit addition against fatty acids and cholesterol oxidation in model systems containing sardine oil (Sardinella brasiliensis) during heating (150 and 180 °C). High temperatures reduced the amount of essential polyunsaturated fatty acids and caused the formation of oxidized products. Total cholesterol oxides content increased from 58.9 ± 0.26 to 577.5 ± 2.14 µg/g oil, after heating at 180 °C. However, aroeira significantly protected lipids from oxidation. Although the synthetic antioxidant applied as standard (butylated hydroxytoluene) showed greater results, it was used in the maximum concentration permitted by Brazilian legislation (0.01%), suggesting that aroeira fruit could be used as a natural antioxidant for the food industry. The protective effect of aroeira may be correlated to its antioxidant capacity and the presence of bioactive compounds which were identified by UHPLC-ESI-MS in the aroeira extract.

Schinus terebenthifolius Raddi extracts: From sunscreen activity toward protection of the placenta to Zika virus infection, new uses for a well-known medicinal plant.

Schinus terebinthifolius Raddi is a well-known medicinal plant native of South America. This species has demonstrated important biological activities such as antihypertensive and vasodilator, antimicrobial, anti-inflammatory and antioxidant. However, no studies have been, so far, reported with the fruits of S. terebinthifolius as a protector of the placenta against Zika virus infection and as sunscreen agents. The present study aimed to investigate new uses for the ethanolic fruit extracts of S. terebinthifolius, from fruits'peel (STPE) and from the whole fruits (STWFE). Zika virus (ZIKV) has been linked to several fetal malformations, such as microcephaly and other central nervous system abnormalities. Thus, the potential of these natural extracts against ZIKV infection was evaluated, using an in vitro method. The photoprotective potential, determined by spectrometry, along with phenolic content, antioxidant capacity, and chemical composition of both extracts were also evaluated. The chemical composition of the extracts was evaluated by HPLC-UV / vis. The cytotoxicity of peel and whole fruit extracts in vero E6 cell lines, in placental cell lines and placental explant cultures were evaluated by the MTT assay. The infectivity of placental cells and explants was evaluated by qRT-PCR and the effects of extracts on ZIKV infection were investigated using HTR-8/SVneo cells, pre-treated with 100 µg mL-1 of STWFE for 1 h, and infected with MR766 (AD) or PE243 (EH) ZIKV strains. STFE and STWFE were well-tolerated by both placental-derived trophoblast cell line HTR-8/SVneo as well as by term placental chorionic villi explants, which indicate absence of cytotoxicity in all analysed concentrations. Two strains of ZIKV were tested to access if pre-treatment of trophoblast cells with the STWFE would protect them against infection. Flow cytometry analysis revealed that STWFE extract greatly reduced ZIKV infection. The extracts were also photoprotective with SPF values equivalent to the standard, benzophenone-3. The formulations prepared in different concentrations of the extracts (5-10 %) had shown maximum SPF values of 32.21. STWFE represents a potential natural mixture to be used in pregnancy in order to restrain placental infection by ZIKV and might potentially protect fetus against ZIKV-related malformations. The extracts exhibited photoprotective activity and some of the phenolic compounds, mainly resveratrol, catechin and epicatechin, are active ingredients in all assayed activities. The development of biotechnological/medical products, giving extra value to products from family farming, is expected, with strong prospects for success.

Aroeira fruit (Schinus terebinthifolius Raddi) as a natural antioxidant: Chemical constituents, bioactive compounds and in vitro and in vivo antioxidant capacity.

Schinus terebinthifolius Raddi fruit, known as Brazilian pepper or aroeira, is a natural source of bioactive compounds. However, studies about the antioxidant and nutritional contribution of this fruit in food systems are limited. Regarding the presence of bioactive compounds, flavonoids showed the highest level (10.33 ± 0.34 mg QE/g), and potential antioxidant components such biflavonoids were determined by ultra-high performance liquid chromatography/electrospray ionization mass spectrometry. The aroeira fruit extract showed antioxidant potential in DPPH (42.68 ± 0.05%), ORAC (43.40 ± 6.22 µM TE/g) and ß- carotene/linoleic acid (61.41 ± 5.30%) assays. Besides that, in vivo analyses demonstrated the ability of aroeira extracts to decrease the damage caused by oxidative stress promoted by H2O2 in Saccharomyces cerevisiae cells. Thus, the presence of phytochemicals with functional properties and the antioxidant capacity of aroeira fruit indicate its use as a potential natural antioxidant for the food industry.

Thin-layer convective drying behaviour of brazilian peppertree leaves / Secagem em camada delgada de folhas de aroeira

Studies on the drying kinetics of medicinal, aromatic and spice plants are a necessary step towards establishing the combination of drying parameters that would not qualitatively and quantitatively alter the quality of the fresh product. The purpose of this study was to investigate the drying kinetics of Brazilian peppertree (Schinus terebinthifolius) leaves and to verify whether the theoretical diffusion and the semi-empirical Lewis drying models were capable of accurately predicting the drying curves of the product at 35, 40, and 45°C, with a dry air mass flow rate of 0.731 kg s-1 m-2. Leaves were collected at 0800 h, 1200 h and 1700 h. The goodness of fit between experimental and predicted values was based on the correlation coefficient, root mean square error, mean absolute percentage error, mean bias error, and fit index. Residual plot analysis was also considered in the selection of the most effective drying model. Temperature had a pronounced effect on mass transfer and drying rate decreased continuously during the course of drying. Leaf collection time had little influence on the drying kinetics. Effective moisture diffusivity was found to increase with temperature. The results indicated that the Lewis model was considered a better predictor of the thin-layer drying behaviour of Brazilian peppertree leaves than the theoretical model based on Fick's second law of diffusion.

Estudos sobre a cinética da secagem de plantas medicinais, aromáticas e condimentares são necessários para o estabelecimento de parâmetros operacionais de secagem de forma a não alterar significativamente a qualidade e o perfil fitoquímico dessas plantas. O objetivo do presente trabalho foi investigar a cinética da secagem das folhas da aroeira-vermelha (Schinus terebinthifolius), além de verificar se o modelo teórico de difusão e o modelo semi-empírico de Lewis são capazes de predizer com acurácia as curvas de secagem do produto a 35, 40 e 45°C, com fluxo de ar seco de 0,731 kg s-1 m-2. As folhas foram coletadas às 8:00, 12:00 e 17:00 h. O grau de adequação dos valores obtidos pelas curvas de secagem àqueles obtidos experimentalmente foi avaliado empregando-se o coeficiente de determinação, a raiz do erro quadrático médio, o erro percentual absoluto médio, o viés médio e o índice de ajuste. A análise da dispersão dos resíduos também foi utilizada na escolha do modelo de predição mais efetivo. Observou-se que a temperatura tem efeito significativo na transferência de massa e a taxa de secagem diminuiu continuamente ao longo da secagem. O horário de coleta das folhas não teve influência significativa sobre a cinética da secagem. A difusividade efetiva da água aumentou com o aumento da temperatura. Os resultados evidenciaram que o modelo de Lewis descreve com maior grau de acurácia a secagem de folhas de aroeira-vermelha que o modelo teórico baseado na Segunda Lei de Difusão de Fick.

Bioprospecting of Diaporthe terebinthifolii LGMF907 for antimicrobial compounds.

Antibiotic-resistant bacteria have been observed with increasing frequency over the past decades, driving the search for new drugs and stimulating the interest in natural products sources. Endophytic fungi from medicinal plants represent a great source of novel bioactive compounds useful to pharmaceutical and agronomical purposes. Diaporthe terebinthifolii is an endophytic species isolated from Schinus terebinthifolius, a plant used in popular medicine for several health problems. The strain D. terebinthifolii LGMF907 was previously reported by our group to produce secondary metabolites with biological activity against phytopathogens. Based on these data, strain LGMF907 was chosen for bioprospecting against microorganisms of clinical importance and for characterization of major secondary metabolites. In this study, different culture conditions were evaluated and the biological activity of this strain was expanded. The crude extracts demonstrated high antibacterial activity against Escherichia coli, Micrococcus luteus, Saccharomyces cerevisiae, methicillin-sensitive Staphylococcus aureus, and methicillin-resistant S. aureus. The compounds diaporthin and orthosporin were characterized and also showed activity against the clinical microorganisms evaluated. This study discloses the first isolation of diaporthin and orthosporin from D. terebinthifolii, and revealed the potential of this endophytic fungus to produce secondary metabolites with antimicrobial activity.

Phenolic Compounds Present Schinus terebinthifolius Raddi Influence the Lowering of Blood Pressure in Rats.

This study identified two phenolic compounds in Schinus terebinthifolius Raddi fruits: naringenin (first report in this species) and gallic acid. Their structures were elucidated by nuclear magnetic resonance (NMR) data (¹H-, 13C-NMR) and a high-performance liquid chromatography (HPLC) technique. A high content of phenolics (659.21 mg of gallic acid equivalents/g of sample-Folin-Ciocalteau method) and total flavonoids (140.69 mg of rutin equivalents/g of sample-aluminum chloride method) were quantified in S. terebinthifolius, as well as high antioxidant activity (77.47%-2,2-diphenyl-1-picrylhydrazyl, DPPH method). The antihypertensive activity related to its phenolic content was investigated. After intravenous infusion in Wistar rats, these phenolics significantly reduced (p < 0.05) the systolic, median, and diastolic arterial pressures of individuals. The rotarod test was performed to determine the mechanism of action of the sample vasorelaxant effect. It was found that its action exceeded that of the positive control used (diazepam). This confirmed the vasodilatory activity exerted by S. terebinthifolius fruits is related to the phenolic compounds present in the plant, which are potent antioxidants and inhibit oxidative stress, mainly in the central nervous system.

Development and Validation of a Simultaneous RP-HPLCUV/DAD Method for Determination of Polyphenols in Gels Containing S. terebinthifolius Raddi (Anacardiaceae).

Topical gels containing extracts of Schinus terebinthifolius have been used to treat bacterial vaginosis. It has been reported that this species has antimicrobial, anti-inflammatory and anti-ulcerogenic properties, which can be attributed to the presence of phenolic compounds. In this work, a sensitive and selective reversed-phase HPLC-UV/DAD method for the simultaneous assay of six polyphenols that could be present in S. terebinthifolius was developed. The method was shown to be accurate and precise. Peak purity and similarity index both exceeded 0.99. Calibration curves were linear over the concentration range studied, with correlation coefficients between 0.9931 and 0.9974. This method was used to determine the polyphenol content of a hydroalcoholic extract and pharmacy-compounded vaginal gel. Although the method is useful to assess the 6 phenolic compounds, some compounds could not be detected in the products. SUMMARY: A sensitive, selective, accurate and precise reversed-phase HPLC-UV/DAD method for the simultaneous assay of six polyphenols in S. terebinthifolius Raddi Abbreviations used: RP-HPLC-UV/DAD: Reverse Phase High Performance Liquid Chromatograph with Ultraviolet and Diode Array Detector, HPLC: High Performance Liquid Chromatograph, HPLC-UV: High Performance Liquid Chromatograph with Ultraviolet Detector, ANVISA: Brazilian National Health Surveillance Agency, LOD: Limit of detection, LOQ: Limit of quantitation.

Differentiation of Brazilian Peppertree (Schinus terebinthifolius Raddi) and Peruvian Peppertree (Schinus molle L.) Fruits by UHPLC-UV-MS Analysis of Their Anthocyanin and Biflavonoid Profiles.

The objectives of this work were to determine the phenolic profile of Schinus terebinthifolius and Schinus molle fruits and to develop a reliable method for the differentiation of these two similar spices both known as pink pepper. Anthocyanins, biflavonoids and gallotannins, some of which are reported for the first time in these species, were identified by UHPLC-UV/vis-MS/MS. Consideration of the relative and absolute amounts of phenolics as well as indicator compounds from 18 samples revealed that the relative amounts of anthocyanins and biflavonoids are the most trustworthy parameters. Principal component analysis and cluster analysis (CA) allowed a grouping of the samples according to their species, showing that the anthocyanins are most important for the identification of species. As a result, authentication of the two Schinus species can be accomplished by UHPLC analysis of the relative amounts of anthocyanins combined with CA.

In vitro Cytotoxicity and Anti-herpes Simplex Virus Type 1 Activity of Hydroethanolic Extract, Fractions, and Isolated Compounds from Stem Bark of Schinus terebinthifolius Raddi.

BACKGROUND: Herpes simplex virus type 1 (HSV-1) is associated with orofacial infections and is transmitted by direct contact with infected secretions. Several efforts have been expended in the search for drugs to the treatment for herpes. Schinus terebinthifolius is used in several illnesses and among them, for the topical treatment of skin wounds, especially wounds of mucous membranes, whether infected or not. OBJECTIVE: To evaluate the cytotoxicity and anti-HSV-1 activity of the crude hydroethanolic extract (CHE) from the stem bark of S. terebinthifolius, as well as its fractions and isolated compounds. MATERIALS AND METHODS: The CHE was subjected to bioguided fractionation. The anti-HSV-1 activity and the cytotoxicity of the CHE, its fractions, and isolated compounds were evaluated in vitro by SRB method. A preliminar investigation of the action of CHE in the virus-host interaction was conducted by the same assay. RESULTS: CHE presented flavan-3-ols and showed anti-HSV-1 activity, better than its fractions and isolated compounds. The class of substances found in CHE can bind to proteins to form unstable complexes and enveloped viruses, as HSV-1 may be vulnerable to this action. Our results suggest that the CHE interfered with virion envelope structures, masking viral receptors that are necessary for adsorption or entry into host cells. CONCLUSION: The plant investigated exhibited potential for future development treatment against HSV-1, but further tests are necessary, especially to elucidate the mechanism of action of CHE, as well as preclinical and clinical studies to confirm its safety and efficacy. SUMMARY: Crude hydroethanolic extract (CHE) presents promising activity against herpes simplex virus type 1 (HSV 1), with selectivity index (SI) = 22.50CHE has flavan-3-ols in its composition, such as catechin and gallocatechinThe fractions and isolated compounds obtained from CHE by bioguided fractionation are less active than the CHE against HSV-1CHE interferes with viral entry process in the host cell and acts directly on the viral particle. Abbreviations used: HSV: Herpes simplex virus, CHE: Crude hydroethanolic extract, WF: Water fraction, AF: Ethyl-acetate fraction, MPLC: Medium-performance liquid chromatography, TLC: Thin-layer chromatography, NMR: Nuclear magnetic resonance, ESI-MS: Electrospray ionization mass spectrometry, SRB: Sulforhodamine B, CPE: Cytopathic effect, CC50: 50% cytotoxic concentration, EC50: 50% effective concentration, PBS: Phosphate-buffered saline.

Anti-inflammatory effect of Schinus terebinthifolius Raddi hydroalcoholic extract on neutrophil migration in zymosan-induced arthritis.

ETHNOPHARMACOLOGICAL RELEVANCE: Schinus terebinthifolius is a species of plant from the Anacardiaceae family, which can be found in different regions of Brazil. Schinus is popularly known as aroeirinha, aroeira-vermelha, or Brazilian pepper. In folk medicine, S. terebinthifolius is used for several disorders, including inflammatory conditions, skin wounds, mucosal membrane ulcers, respiratory problems, gout, tumors, diarrhea and arthritis. According to chemical analyses, gallic acid, methyl gallate and pentagalloylglucose are the main components of hydroalcoholic extracts from S. terebinthifolius leaves. In the present study, we demonstrated the ability of a hydroalcoholic extract to inhibit cell migration in arthritis and investigated the mechanisms underlying this phenomenon. MATERIALS AND METHODS: The anti-inflammatory effect of S. terebinthifolius hydroalcoholic leaf extract (ST-70) was investigated in a zymosan-induced experimental model of inflammation. Male Swiss and C57Bl/6 mice received zymosan (100 µg/cavity) via intra-thoracic (i.t.) or intra-articular (i.a.) injection after oral pre-treatment with ST-70. The direct action of ST-70 on neutrophils was evaluated via chemotaxis. RESULTS: ST-70 exhibited a dose-dependent effect in the pleurisy model. The median effective dose (ED50) was 100mg/kg, which inhibited 70% of neutrophil accumulation when compared with the control group. ST-70 reduced joint diameter and neutrophil influx for synovial tissues at 6h and 24h in zymosan-induced arthritis. Additionally, ST-70 inhibited synovial interleukin (IL)-6, IL-1ß, keratinocyte-derived chemokine (CXCL1/KC) and Tumor Necrosis Factor (TNF)-α production at 6h and CXCL1/KC and IL-1ß production at 24h. The direct activity of ST-70 on neutrophils was observed via the impairment of CXCL1/KC-induced chemotaxis in neutrophils. Oral administration of ST-70 did not induce gastric damage. Daily administration for twenty days did not kill any animals. In contrast, similar administrations of diclofenac induced gastric damage and killed all animals by the fifth day. CONCLUSIONS: Our results demonstrated significant anti-inflammatory effects of ST-70, suggesting a putative use of this herb for the development of phytomedicines to treat inflammatory diseases, such as joint inflammation.

Chemical composition of essential oil from ripe fruit of Schinus terebinthifolius Raddi and evaluation of its activity against wild strains of hospital origin.

The essential oil (EO) composition of ripe fruit of S. terebinthifolius Raddi was analyzed by GC-MS. The oil extraction yielded 6.54 ± 1.06% (w/w). Seventeen compounds were identified, accounting for 91.15% of the total oil, where monoterpenes constituted the main chemical class (85.81%), followed by sesquiterpenes (5.34%). The major monoterpene identified was δ-3-carene (30.37%), followed by limonene (17.44%), α-phellandrene (12.60%) and α-pinene (12.59%). Trans-caryophyllene (1.77%) was the major sesquiterpene identified. The antibacterial activity of the essential oil was evaluated against wild strains of hospital origin (Escherichia coli, Pseudomonas sp., Klebsiella oxytoca, Corynebacterium sp., Staphylococcus aureus, Enterobacter sp., Enterobacter agglomerans, Bacillus sp., Nocardia sp. and Streptococcus group D). The essential oil of the ripe fruit of S. terebinthifolius Raddi has shown to be active against all tested wild strains, with minimum inhibitory concentration ranging from 3.55 µg/mL to 56.86 µg/mL. However, it has revealed some differences in susceptibility: the general, Gram-positive species showed greater sensitivity to the action of EO, which is probably due to the lower structural complexity of their cell walls.

Chemical composition of essential oil from ripe fruit of Schinus terebinthifolius Raddi and evaluation of its activity against wild strains of hospital origin

The essential oil (EO) composition of ripe fruit of S. terebinthifolius Raddi was analyzed by GC-MS. The oil extraction yielded 6.54 ± 1.06% (w/w). Seventeen compounds were identified, accounting for 91.15% of the total oil, where monoterpenes constituted the main chemical class (85.81%), followed by sesquiterpenes (5.34%). The major monoterpene identified was δ-3-carene (30.37%), followed by limonene (17.44%), α-phellandrene (12.60%) and α-pinene (12.59%). Trans-caryophyllene (1.77%) was the major sesquiterpene identified. The antibacterial activity of the essential oil was evaluated against wild strains of hospital origin (Escherichia coli, Pseudomonas sp., Klebsiella oxytoca, Corynebacterium sp., Staphylococcus aureus, Enterobacter sp., Enterobacter agglomerans, Bacillus sp., Nocardia sp. and Streptococcus group D). The essential oil of the ripe fruit of S. terebinthifolius Raddi has shown to be active against all tested wild strains, with minimum inhibitory concentration ranging from 3.55 μg/mL to 56.86 μg/mL. However, it has revealed some differences in susceptibility: the general, Gram-positive species showed greater sensitivity to the action of EO, which is probably due to the lower structural complexity of their cell walls.

Percentual de suplementação de fonte taninífera na ração concentrada de caprinos jovens sobre o desempenho e carga parasitária / Tanniferous plant supplementation percentage in young goats concentrates diet on perfermance and parasite contro

O presente estudo buscou determinar o percentual de adição de torta de pimenta rosa (TPR) na dieta de caprinos jovens sobre o desempenho e carga parasitária. Foram utilizados 25 caprinos jovens da raça Saanen, distribuídos em um delineamento inteiramente casualizado em grupos de cinco tratamentos e cinco repetições, constituídos por grupos: não suplementados com TPR; suplementados com 15% TPR; suplementados com 30% TPR; suplementado com 45% TPR; e suplementado com 60% TPR. O aumento dos níveis de suplementação de pimenta rosa resultou em efeito quadrático sobre o consumo voluntário de matéria seca (CVMS), peso metabólico e ganho em peso diário (P≤0,05). Para o CVMS, o comportamento quadrático (P≤0,05) revelou ponto de mínimo de 67,1g kg0,75 dia-1 no percentual de 59,8% de TPR; para o peso metabólico, um ponto máximo de 8,5kg0,75 no percentual de 15,5% de TPR; e, para o ganho em peso diário, um ponto máximo de 0,107g dia-1 no percentual de 19,6% de TPR na dieta. Em relação à influencia da TPR sobre a carga parasitária, observou-se efeito quadrático dos níveis de suplementação de TPR na contagem de ovos por grama de fezes (OPG) (P≤0,05), com ponto de mínimo estimado em 1,0Log10 no nível de 35,8% TPR na dieta, representando o limite de resposta do TPR. Além da redução no OPG, houve um decréscimo linear na contagem de oocistos por grama de fezes (OOPG) com o incremento nos níveis de TPR da dieta. Conclui-se que a adição de 20-30% TPR possibilitou o controle de parasitos gastrointestinais com máximo desempenho animal.

The aim of this study was to evaluate the pink pepper supplementation (PPS) in young goats concentrate diet on performance parameters. Twenty five female Saanen goats were divided into five groups having five goats in each treatment. In a completely randomized design were given 0%, 15%, 30%, 45% and 60% of pink pepper supplementation in the diet. The increase levels of pink pepper supplementation resulted in a quadratic effect on voluntary intake of dry matter, metabolic weight and daily weight gain (P≤0.05). For voluntary intake of dry matter, the quadratic effect (P≤0.05) revealed minimum point of 67.1 (g kg0.75 dia-1) in the percentage of 59.8% PPS; for metabolic weight a peak of 8. 5kg0.75 in the percentage of 15.5% PPS, and the daily weight gain a peak of 0.107g day-1 in the percentage of 19.6% PPS in the diet. Regarding the effect of PPS on the parasite load, there was a quadratic effect of dietary supplementation of the PPS on egg count per gram of feces (P≤0.05), with minimum point estimated at 1.0log10 at 35.8% of PPS in the diet, representing the limit of PPS response. There was a linear decrease on oocyst count per gram feces with increased levels of PPS 's diet. It was concluded that the supplementation up to 20-30% of pink pepper was able to control of gastrointestinal parasites with maximum animal performance.

Sinergia entre aditivo vegetal e níveis crescentes de promotores de crescimento sobre o desempenho produtivo e morfometria intestinal de frangos de corte / Synergy between vegetal additive with increase levels of growth promoter on the performance and intestinal morphometry of broilers chickens

Objetivou-se avaliar o efeito da adição de níveis crescentes de antibióticos associados ao farelo integral de pimenta rosa (FIPR) sobre o desempenho produtivo e morfometria intestinal de frangos corte. Utilizaram-se 528 pintos Cobb, machos, distribuídos em um delineamento inteiramente casualizado, com seis tratamentos e quatro repetições de 22 animais cada, constituído pelos grupos T1 - Controle Negativo (CN) - Dieta basal sem FIPR e sem antibióticos; T2 - Controle Positivo (CP) - Dieta Basal com 1,2% FIPR; T3 - CP + 11ppm bacitracina zinco e 17ppm salinomicina; T4 - CP + 22ppm bacitracina zinco e 34ppm salinomicina; T5 - CP + 33ppm bacitracina zinco e 51ppm salinomicina; T6 - CP + 45ppm bacitracina zinco e 67ppm salinomicina. No período de 1-43 dias de idade, observou-se que a adição de FIPR com ou sem antibiótico reduziu (P≤0,05) o consumo e melhorou a conversão alimentar, comparado com CN. Verificou-se que os frangos alimentados com FIPR associado aos antibióticos, em valores acima de 22mg kg-1 de bacitracina de zinco e 34mg kg-1 de salinomicina, tiveram maiores alturas de vilosidades intestinais que o CN (P≤0,05). O FIPR pode ser utilizado em substituição aos antibióticos sem queda de desempenho animal e com a manutenção da viabilidade e do fator de produção.

The aim of this research was to evaluate the effects of the increase levels of antibiotics associated with the pink pepper meal (PPM) on animal performance and intestinal morphometry of broilers jejunum. A total of 528 day-old male chicks, Cobb 500, distributed in a randomized design of six treatments and four replicates of 22 birds each: T1 - Negative Control (NC) - basal diet without PPM or antibiotics, T2 - Positive Control (PC) - basal diet with 1,2% PP; T3 - PC + 11ppm zinc bacitracin and 17ppm salinomycin, T4 - PC + 22ppm zinc bacitracin and 34ppm salinomycin, T5 - PC + 33ppm zinc bacitracin and 51ppm salinomycin, T6 - PC + 45ppm zinc bacitracin and 67ppm salinomycin. In period of 1-43 days-old, it was observed that the use of PPM with or without antibiotcs reduced feed intake and improved feed:gain ratio compared with NC (P≤0,05). It was observed that the broilers fed diet with antibiotics values of up to 22mg kg-1 of zinc bacitracin and 34mg kg-1 of salinomycin associated with the PPM had the higher villus height compared with NC. The PPM can be used to replace antibiotic growth promoters without decrease on animal performance, viability and production factor.

Alcohol extract of Schinu sterebinthifolius raddi (anacardiaceae) as a local antimicrobial agent in severe autogenously fecal peritonitis in rats

PURPOSE: To develop an alcoholic extract of the inner bark of the Schinus terebinthifolius raddi and to test its impact on autogenously fecal peritonitis in Wistar rats. METHODS: The inner bark of the Schinus terebinthifolius raddi was kept for seven days in 70% ethanol alcohol. The total elimination of the solvent was performed in a rotary evaporator under reduced pressure at 55-60°C. Four milliliter of this extract was injected, after 24 h, into the abdominal cavity of six out of eight survival rats that underwent autogenously fecal peritonitis with five milliliter of 10% filtered fecal suspension. They were clinically followed up for 45 days when they were euthanized. The necropsy findings (inventory) of the abdominal and thorax cavities were inspected and the main findings were recorded and photographed. The investigation was approved by the Ethics Committee. RESULTS: Two out of six survival rats that were critically ill after 24 h died within the 12 h after the extract injection into the abdominal cavity. Four rats that were also critically ill recovered and gradually became healthy, eating well, regaining weight and moving normally in the cage. At 45 days post severe peritonitis the necropsy findings revealed few signs of residual infection on the abdominal and thorax cavities. There were no bowel adhesions. CONCLUSION: The impact of alcoholic extract of the inner bark of the Schinus terebinthifolius raddi was considered very positive and promising as natural local antiseptic against very severe peritonitis in Wistar rats. .

Óleo de aroeira vermelha e de suplementação de vitamina E em substituição aos promotores de crescimento sobre a microbiota intestinal de frangos de corte / Supplementation of pink pepper oil and vitamin E in replacement to antibiotic growth promoters in the broilers diet on response of ileal microbiota

Objetivou-se avaliar o uso de óleo de aroeira-vermelha (OAV) e da suplementação de vitamina E (VitE) sobre a microbiota intestinal de frangos de corte. Utilizou-se 400 pintos de corte distribuídos em um delineamento inteiramente casualizado, em grupos de cinco tratamentos e cinco repetições: dieta sem antimicrobiano; dieta com antimicrobiano; dieta com 0,4% OAV; dieta com 200mg de VitE kg-1; dieta com 0,4% OAV e 200mg de VitE kg-1. A adição de OAV reduziu o peso relativo dos intestinos (P<0,05). Verificou-se que o controle negativo teve a menor contagem de bactérias do gênero Lactobacillus spp. em contraste com os demais grupos (P<0,05). Observou-se que o uso de OAV com ou sem adição de VitE reduziu significativamente a contagem de bactérias do gênero Staphylococcus spp e E. coli, quando comparado com o grupo não tratado com o promotor de crescimento (P<0,05). Para os Staphylococcus spp coagulase-positivos, verificou-se que a concentração inibitória mínima foi de 14,72-117,75mg mL-1 para amostras isoladas de frangos de corte tratados com OAV + VitE, enquanto que para tratados com ou sem promotor de crescimento foi de 117,75 e de 29,44-235,5mg mL-1. Concluiu-se que a inclusão de OAV resultou em modulação da microbiota intestinal.

The aim was to evaluate the supplementation of pink pepper oil (PPO) and vitamin E on the intestinal microbiota of broiler chickens. A total of 400 day-old male chicks distributed in a randomized design in groups of five treatments and five replicates: diet without antimicrobial; diet with antimicrobial; diet with 0.4% PPO; diet with 200mg vitamin E kg-1; diet with 0.4% PPO and 200mg vitamin E kg-1 The supply of PPO in the diet reduced the relative weight of the intestines (P<0.05). It was verified that the negative control group had the lowest bacteria count of Lactobacillus in contrast to the other groups (P<0.05). It was also observed that the use of PPO with or without vitamin E supplementation significantly reduced the bacterial count of Staphylococcus spp and E. coli when compared with the untreated group with the growth promoter (P<0.05). For Staphylococcus spp. coagulase-positive, it was found that the minimum inhibitory concentration was 14,72-117,75mg mL-1 for isolates from broilers treated with PPO + vitamin E, while for treated with or without growth promoter was 117.75 and 29,44-235,5mg mL-1. It was concluded that the inclusion of PPO resulted in modulation of intestinal microbiota.