ABSTRACT
Although the pour plate method is widely employed in microbiological quality control, it has certain drawbacks, including having to melt the culture medium before seeding. In this study, the preparation of the culture medium was modified by using a lower concentration of agar (10 g/L), which was separated from the nutrients during sterilization. The new protocol was assessed in media frequently used in microbiological quality control of food, cosmetics, and pharmaceutical products, with tryptic soy agar (TSA), Sabouraud 4% dextrose agar (SDA), and violet red bile glucose agar (VRBG). In comparison with the conventionally produced media, the modifications significantly improved the growth of Saccharomyces cerevisiae in SDA, Staphylococcus aureus, Salmonella enterica subsp. enterica serovar Typhimurium, and Candida albicans in TSA and Escherichia coli ATCC 8739 and ATCC 25922 and S. Typhimurium in VRBG. The modified VRBG was also more selective for Pseudomonas aeruginosa. Regarding physicochemical properties, a significantly lower pH was observed in TSA and VRBG and lower strength values in TSA. Sterilizing agar separately from the other components of the medium and reducing the agar concentration to 10 g/L can improve microorganism growth and enhance the selectivity of differential media in the pour plate method. These modifications could facilitate the automation of this culture technique. IMPORTANCE In the era of rapid microbiological methods, there is a need to improve long-established culture techniques. Drawbacks of the pour plate method include having to melt each medium separately before seeding. For this technique, we demonstrate that separating the agar from the other components of commonly used media during sterilization and reducing the agar concentration to 10 g/L can enhance microbial growth. The new protocol could have advantages in routine laboratory practice because less agar is required and the same molten agar suspension can be used to prepare different media. Moreover, these modifications could facilitate the automation of the pour plate method.
Subject(s)
Microbiological Techniques , Salmonella typhimurium , Agar , Culture Media , Escherichia coli , SterilizationABSTRACT
Introducción: la técnica de separación de componentes o técnica de Ramírez, consiste en la separación de los componentes anatómicos de la pared abdominal anterior, consiguiendo avances de 4 a 6 cm de cada lado de los bordes del defecto con un cierre de menor tensión en el tratamiento de afecciones parietales complejas. Objetivo: valorar los resultados del tratamiento de afecciones parietales complejas con la técnica de separación de componentes con refuerzo de malla de polipropileno. Material y métodos: estudio observacional, descriptivo, prospectivo, longitudinal de pacientes operados de forma electiva, portadores de afección parietal compleja, utilizando la Técnica de Separación de Componentes con refuerzo de malla de polipropileno, en la II Cátedra de Clínica Quirúrgica del Hospital de Clínicas, Facultad de Ciencias Médicas de la Universidad Nacional de Asunción, en mayo del 2010 a febrero del 2014...
Introduction: the technique of separation of components or technique of ramirez, in the separation of the anatomical components of the anterior abdominal wall, getting advances from 4 to 6 cm on each side of the edges of the defect with a lower tension locks in the treatment of complex wall conditions. Objective: to evaluate the results of complex parietal conditions with the technique of separation of components with polypropylene mesh reinforcement. Patients and methods: observational, descriptive, prospective, longitudinal in patients Electively, carriers of parietal complex condition, using the technique of separation of Componemtes reinforced polypropylene mesh, at the II Department of surgical clinic of the Hospital de Clínicas, Faculty of medical sciences of the National University of Asunción, in May 2010 to February of 2014...
Subject(s)
Male , Female , Humans , Adult , General Surgery , PolypropylenesABSTRACT
Key steps in managing patients with enterocutaneous fistulation and an abdominal wall defect include dealing effectively with abdominal sepsis and providing safe and effective nutritional support and skin care, then assessing intestinal and abdominal anatomy, before undertaking reconstructive surgery. The complexity, cost, and morbidity associated with such cases justifies creation of specialized centers in which gastroenterologic, hernia, and plastic surgical expertise, as well as experienced wound and stoma nursing and nutritional and psychological support, can be made available for patients with these challenging problems.
