Odd-Paired is Involved in Morphological Divergence of Snail-Feeding Beetles.

Body shape and size diversity and their evolutionary rates correlate with species richness at the macroevolutionary scale. However, the molecular genetic mechanisms underlying the morphological diversification across related species are poorly understood. In beetles, which account for one-fourth of the known species, adaptation to different trophic niches through morphological diversification appears to have contributed to species radiation. Here, we explored the key genes for the morphological divergence of the slender to stout body shape related to divergent feeding methods on large to small snails within the genus Carabus. We show that the zinc-finger transcription factor encoded by odd-paired (opa) controls morphological variation in the snail-feeding ground beetle Carabus blaptoides. Specifically, opa was identified as the gene underlying the slender to stout morphological difference between subspecies through genetic mapping and functional analysis via gene knockdown. Further analyses revealed that changes in opa cis-regulatory sequences likely contributed to the differences in body shape and size between C. blaptoides subspecies. Among opa cis-regulatory sequences, single nucleotide polymorphisms on the transcription factor binding sites may be associated with the morphological differences between C. blaptoides subspecies. opa was highly conserved in a wide range of taxa, especially in beetles. Therefore, opa may play an important role in adaptive morphological divergence in beetles.

Evolutionary morphology of haptoral anchors in monogenoids (Dactylogyridae) of marine catfish (Siluriformes: Ariidae) from the Atlantic coast of South America.

Exploring the phylogenetic signal of morphological traits using geometric morphometry represents a powerful approach to assess the relative weights of convergence and shared evolutionary history in shaping species' forms. We evaluated the phylogenetic signal in shape and size of ventral and dorsal haptoral anchors of 10 species of monogenoids (Hamatopeduncularia, Chauhanellus and Susanlimocotyle) occurring in marine catfish (Siluriformes: Ariidae) from the Atlantic coast of South America. The phylogenetic relationships among these species were mapped onto the morphospaces of shape and size of dorsal and ventral anchors. Two different tests (squared change-parsimony and Kmult) were applied to establish whether the spatial positions in the phylomorphospace were influenced by phylogenetic relationships. A significant phylogenetic signal was found between anchor form and parasite phylogeny. Allometric effects on anchor shape were non-significant. Phylogenetically distant species on the same host differed markedly in anchor morphology, suggesting little influence of host species on anchor form. A significantly higher level of shape variation among ventral anchors was also found, suggesting that the evolutionary forces shaping ventral anchor morphology may operate with differing intensities or exhibit distinct mechanisms compared to their dorsal counterparts. Our results suggest that phylogenetic relationships were a key driver of changes in shape (but not size) of anchors of monogenoids of South American ariids. However, it seems that the emergence of the digitiform haptor in Hamatopenducularia and in some species of Chauhanellus played an important role in the reduction in anchor size and may cause secondary losses of anchors in other groups of monogenoids.

Achieving the ideal balance between biological and mechanical requirements in composite bone scaffolds through a voxel-based approach.

Achieving successful bone regeneration necessitates the design of scaffolds that meet diverse biological and mechanical requirements, often leading to conflicts in the design parameters. A key conflict arises between scaffold porosity and stiffness. Increasing porosity facilitates cell infiltration and nutrient exchange, promoting bone regeneration. However, higher porosity compromises scaffold stiffness, which is crucial for providing structural support in the defective region. Furthermore, appropriate scaffold stiffness is crucial for preventing stress shielding. Conventional geometry-based design methods utilizing single-phase materials have limited flexibility in resolving such conflicts. To address this challenge, we propose a voxel-based method for designing composite scaffolds composed of hydroxyapatite (HA) and polylactic acid (PLA). Our strategy involves first satisfying primary biological requirements by selecting appropriate porosity, pore shape, and size. Subsequently, scaffold stiffness requirements are met by selecting suitable phase materials and tuning their contents. The study demonstrates that the voxel-based approach effectively balances both biological and mechanical requirements in scaffold design. This method addresses the limitations of traditional designs by achieving an optimal balance between porosity and stiffness, which is crucial for scaffold performance in biomedical applications. Moreover, the scaffolds designed using this method can be manufactured using voxel-based 3D printing technology, which is emerging in the field. Future advancements in voxel-based 3D printing technology will further enhance the feasibility and practicality of this approach for bone tissue engineering applications.

Variation in flower size and shape of Impatiens capensis is correlated with urbanization in Montreal, Canada.

Urbanization is changing the conditions in which many species live, forcing them to adjust to these novel environments. Floral size and shape are critical traits for the reproduction of plants pollinated by animals as they are involved in the attraction of pollinators and in efficient pollination. Variation in size and shape could be affected by urbanization via its modification of the abiotic environment (habitat fragmentation, water availability, temperature, soil properties), or via its impact on the biotic environment of plants (pollination, herbivory). Although numerous studies have assessed the impact of urbanization on pollinator communities and many plant traits, few have investigated its impact on floral size and shape while quantifying the proportion of the total urbanization effect that is due to biotic interactions. In this study, we tested if urbanization and pollinator visitation rates affect the flower shape of the spotted jewelweed, Impatiens capensis. We quantified the size and shape of flowers in frontal and profile views using geometric morphometrics for 228 individuals from six populations from the region of Montreal, Canada. Pollinator visitation rates were estimated at each site and the main pollinators were found to be bumblebees, honeybees and hummingbirds. We found that floral size and shape are significantly correlated with urbanization as measured by the amount of vegetation in the surrounding environment of the plants (mean normalized vegetation index, NDVI) and by the visitation rates of bumblebees and honey bees. Partitioning of the total flower shape variation suggests that urbanization affects flower shape through abiotic factors and via its impact on pollinator visitation rates. While further studies from other cities are necessary to confirm the role of urbanization in shaping the floral shape of I. capensis, these results support the idea that urbanization could affect flower shapes.

Anomaly Detection in the Production Process of Stamping Progressive Dies Using the Shape- and Size-Adaptive Descriptors.

In the production process of progressive die stamping, anomaly detection is essential for ensuring the safety of expensive dies and the continuous stability of the production process. Early monitoring processes involve manually inspecting the quality of post-production products to infer whether there are anomalies in the production process, or using some sensors to monitor some state signals during the production process. However, the former is an extremely tedious and time-consuming task, and the latter cannot provide warnings before anomalies occur. Both methods can only detect anomalies after they have occurred, which usually means that damage to the die has already been caused. In this paper, we propose a machine-vision-based method for real-time anomaly detection in the production of progressive die stamping. This method can detect anomalies before they cause actual damage to the mold, thereby stopping the machine and protecting the mold and machine. In the proposed method, a whole continuous motion scene cycle is decomposed into a standard background template library, and the potential anomaly regions in the image to be detected are determined according to the difference from the background template library. Finally, the shape- and size-adaptive descriptors of these regions and corresponding reference regions are extracted and compared to determine the actual anomaly regions. The experimental results indicate that this method can achieve reasonable accuracy in the detection of anomalies in the production process of stamping progressive dies. The experimental results demonstrate that this method not only achieves satisfactory accuracy in anomaly detection during the production of progressive die stamping, but also attains competitive performance levels when compared with methods based on deep learning. Furthermore, it requires simpler preliminary preparations and does not necessitate the adoption of the deep learning paradigm.

Relationship between Key Environmental Factors and the Architecture of Fruit Shape and Size in Near-Isogenic Lines of Cucumber (Cucumis sativus L.).

Fruit shape and size are complex traits influenced by numerous factors, especially genetics and environment factors. To explore the mechanism of fruit shape and size development in cucumber, a pair of near-isogenic lines (NIL) Ln35 and Ln37 were used. The fruit length and diameter, cell length and diameter, and related gene expression were measured. Both the fruit length, diameter, and cell length and diameter showed sigmate curves in the two lines. The cell length and diameter were significantly positively correlated with fruit length and diameter both in two lines. The expression of CsACS2 and CsLNG showed significant positive correlations with fruit length and diameter increment in Ln35, and there was no correlation in Ln37. Furthermore, there were significant positive correlations between fruit size and thermal effectiveness (TE), as well as between fruit size and photosynthetic active radiation (PAR), both in two lines. Two models using logistic regression were formulated to assess the relationships among fruit length and diameter in Ln35 and Ln37, respectively, based on thermal effectiveness and photosynthetic active radiation (TEP). The coefficient R2 values of the models were 0.977 and 0.976 in Ln35, and 0.987 and 0.981 in Ln37, respectively. The root mean square error (RMSE) was 12.012 mm and 4.338 mm in Ln35, and 5.17 mm and 7.082 mm in Ln37, respectively, which illustrated the accurate and efficient of these models. These biologically interpreted parameters will provide precision management for monitoring fruit growth and forecasting the time of harvesting under different temperatures and light conditions.

Control of Grain Shape and Size in Rice by Two Functional Alleles of OsPUB3 in Varied Genetic Background.

Grain shape and size are key determinants of grain appearance quality and yield in rice. In our previous study, a grain shape QTL, qGS1-35.2, was fine-mapped using near-isogenic lines (NILs) derived from a cross between Zhenshan 97 (ZS97) and Milyang 46 (MY46). One annotated gene, OsPUB3, was found to be the most likely candidate gene. Here, knockout and overexpression experiments were performed to investigate the effects of OsPUB3 on grain shape and size. Four traits were tested, including grain length, grain width, grain weight, and the ratio of grain length to width. Knockout of OsPUB3 in NILZS97, NILMY46, and another rice cultivar carrying the OsPUB3MY46 allele all caused decreases in grain width and weight and increases in the ratio of grain length to width. Results also showed that the magnitude of the mutational effects varied depending on the target allele and the genetic background. Moreover, it was found that NILZS97 and NILMY46 carried different functional alleles of OsPUB3, causing differences in grain shape rather than grain weight. In the overexpression experiment, significant differences between transgenic-positive and transgenic-negative plants were detected in all four traits. These results indicate that OsPUB3 regulates grain shape and size through a complex mechanism and is a good target for deciphering the regulatory network of grain shape. This gene could be used to improve grain appearance quality through molecular breeding as well.

UNetGE: A U-Net-Based Software at Automatic Grain Extraction for Image Analysis of the Grain Size and Shape Characteristics.

The shape and the size of grains in sediments and soils have a significant influence on their engineering properties. Image analysis of grain shape and size has been increasingly applied in geotechnical engineering to provide a quantitative statistical description for grain morphologies. The statistic robustness and the era of big data in geotechnical engineering require the quick and efficient acquirement of large data sets of grain morphologies. In the past publications, some semi-automation algorithms in extracting grains from images may cost tens of minutes. With the rapid development of deep learning networks applied to earth sciences, we develop UNetGE software that is based on the U-Net architecture-a fully convolutional network-to recognize and segregate grains from the matrix using the electron and optical microphotographs of rock and soil thin sections or the photographs of their hand specimen and outcrops. Resultantly, it shows that UNetGE can extract approximately 300~1300 grains in a few seconds to a few minutes and provide their morphologic parameters, which will ably assist with analyses on the engineering properties of sediments and soils (e.g., permeability, strength, and expansivity) and their hydraulic characteristics.

Establishing growth charts for proposed body shape and size index of the Pakistani population, using quantile regression approach.

BACKGROUND: Obesity leads to other fatal diseases like diabetes, cardiovascular diseases, depression, and some forms of cancer. Still, the well-known tool to measure obesity is the body mass index. But it usually failed in the measurement of adipose tissues. So, we present a novel anthropometric measure, called body shape and size index which is developed by the combination of major anthropometric determinants: body surface area, body mass index, weight, and height. METHODS: This study is based on cross-sectional data consisting of 7224 individuals that were taken from the city Multan, Punjab, Pakistan. All the individuals, both males, and females, of age 2 years and above were included in the study except the pregnant women. The variables included in this study are gender, area (urban and rural), age (years), weight (kg), and height (meters). Growth charts of quantile regression are used for the inferential analysis of data. Comparison of proposed body shape and size index at different obesity levels has also been made to access the relationship of proposed body shape and size index with obesity. RESULTS: The results show that the proposed body shape and size index has a great association with body surface area, body mass index, weight, height, and age. The proposed body shape and size index has a high negative association with body surface area, moderate negative association with body mass index and weight, and low negative association with height and age. According to growth charts of body shape and size index, after the age of 25 years, body shape and size index curves go upward while it smoothly goes downward at the age of 50 years but decreases in earlier ages. Body shape and size index showed a significant association with body shape and body size (body development) at the same time. CONCLUSION: Body shape and size index is found, generally linear with age, and increased with decreasing body mass index and body surface area. The proposed index has an indirect relationship with obesity. Body shape and size index with low values indicates a high risk of obesity. While, however, body shape and size index with high values indicates a low risk of obesity. Applications of the proposed body shape and size index are also presented in statistical modeling.

Noble Metals Based Bimetallic and Trimetallic Nanoparticles: Controlled Synthesis, Antimicrobial and Anticancer Applications.

Noble bimetallic and trimetallic nanoparticles (NBT-NPs) have superior biomedical applications as compared to their monometallic counterparts. The performance of these nanomaterials depends on their composition, shape and size. Hence, the controlled-synthesis of these nanomaterials is a hot area of research. Till date, no review article in the literature accounts regarding the controlled-synthesis and biomedical applications related to morphology, optimum composition, biocompatibility and versatile chemistry of NBT-NPs. Taking this into contemplation, an effort was made to provide a clear insight into the morphology-controlled synthesis and size/shape-dependent anticancer and bactericidal applications of NBT-NPs. Chemical reduction method for the controlled-synthesis of NBT-NPs is reviewed critically. Furthermore, the potential role of various reaction parameters such as time, reducing agents, stabilizing/capping agents, nature/concentration of precursors, temperature and pH in the shape/size-controlled synthesis of these nanomaterials are discussed. In the second part of this article, anticancer and bactericidal applications of the NBT-NPs are reviewed and the influences of optimum composition, size, surface structure, versatile chemistry and synergism are studied. Finally, the current challenges in the controlled-synthesis and biomedical applications of these nanomaterials, and prospects to resolve related issues are discussed. HighlightsChemical reduction method for the synthesis of NBT-NPs is reviewed.The influences of parameters on the control synthesis of NBT-NPs are discussed.Antibacterial and anticancer applications and cytotoxicity of NBT-NPs are reviewed.Possible solutions for the key challenges are discussed.Outlooks about the synthesis and biomedical applications of NBT-NPs are discussed.

Role of the amine and phosphine groups in oleylamine and trioctylphosphine in the synthesis of copper chalcogenide nanoparticles.

The effect of the functional groups of capping agents was investigated in the synthesis of copper selenide, copper sulphide and copper oxide nanoparticles using oleylamine (OLA) and trioctylphosphine (TOP). These capping molecules have demonstrated their ability to act as reducing agents, surfactants, solvents and enhancement of colloidal stabilization. They also offer electron donating abilities from the two group 5A elements, P and N. Nitrogen atom in an amine group possess stronger surface interactions and higher basicity than P atom in the phosphines. Copper chalcogenide nanoparticles were prepared using Hot-injection method and characterized using UV/Vis spectroscopy, TEM and XRD. The optical and structural properties of the yielded nanoparticles showed dependence on the type of capping interactions from the two agents. Nanoparticles synthesized using TOP produced two phases whereas a single phase was observed from OLA as confirmed by XRD. Although TOP and OLA exhibit similar features, but their affinity to metals differs resulting to significant different morphology and crystallinity of the produced nanoparticles. Amine group has higher affinity for protons than phosphine due to the lone pair of electrons it possesses which it easily donates to H+ compared to phosphine. The high proton affinity of oleylamine makes it interact faster than trioctylphosphine. OLA in overall produced larger particle sizes compared to TOP but generated a wider variety of shapes.

Relationship between Seed Morphological Traits and Ash and Mineral Distribution along the Kernel Using Debranning in Durum Wheats from Different Geographic Sites.

Debranning was applied to durum wheat to the study the relationship between kernel shape and size, and ash and mineral distribution having implications for semolina yield. To this aim four durum wheat genotypes carried out over three environments were selected to determine the morphological and yield traits as well as the distribution along the kernel of the ash, macro- (Na, K, P, Ca, and Mg), and micro-elements (Mn, Fe, Cu, Zn, and Mo). A descendent ash gradient within the kernel reflects the decreases in the minerals that occurred during debranning. Perciasacchi with high seed weight (TKW) and greater thickness followed by Cappelli showed a more uniform distribution of ash content along the kernels. High r Pearson coefficient (p < 0.01) showed an inverse relationship between thickness and ash decay. Since thickness was strongly affected by the genotype, it could represent a useful trait for breeding programs to predict the milling quality.

Bioinspired morphology-controlled silver nanoparticles for antimicrobial application.

Phytochemicals sources have been extensively used as reducing and capping agents for synthesis of nanoparticles (NPs). However, morphology-controlled synthesis and shape/size dependent applications of these NPs still need to be explored further, and there is a need to develop a way in which particular and optimized phytochemicals result in the desired NPs in lesser time and cost with higher reproducibility rate. The present study is focused on morphology-controlled synthesis and shape/size dependent application of silver NPs based on the fractionated phytochemicals of Elaeagnus umbellata extract (EU). Unlike other approaches, in this study the reaction parameters such as time, temperature, pH, stirring speed and concentration of the precursor solutions were not altered during the optimization process. The fractionated phytochemicals were used separately for the synthesis of AgNPs, and the synthesized NPs were characterized by UV-visible, FT-IR, atomic force microscopy (AFM) and scanning electron microscopy (SEM). Our findings suggested that the constituents of the extract fractions varied with the selection of the extraction solvent, and the shape/size, bactericidal properties and toxicity of the NPs have a strong correlation with the phytochemicals of the plant extract. The fractionated phytochemicals present in the water fractions (EUW) resulted in monodispersed spherical AgNPs in the size about 40 nm. The NPs have significant stability in physiological conditions (i.e. temperature, pH and salt), have good antibacterial activity, and were found to be non-toxic. Furthermore, AFM and SEM analysis exposed that the NPs killed the bacteria by disturbing the cellular morphology and releasing the cellular matrix. Our results justify the use of different fractions of plant extract to obtain detail implications on shape, size, antibacterial potential and toxicity of AgNPs. This is the first step in a controllable, easy and cheap approach for the synthesis of highly stable, uniform, non-toxic and bactericidal AgNPs using five fractions of EU. The findings suggested that the synthesized NPs, particularly from EUW, could be used in pharmaceutical and homeopathic industry for the development of antibacterial medications.

Morphological Characteristics of Dimples of Ductile Fracture of VT23M Titanium Alloy and Identification of Dimples on Fractograms of Different Scale.

The authors developed a method for the automated detection and calculation of quantitative parameters of dimples of ductile fracture on the digital images of fracture surfaces obtained at different scales. The processing algorithm of fractographic images was proposed, which allowed high quality recognition of the shape and size of dimples to be achieved, taking into account the morphological features of their digital images. The developed method for identifying dimples of various physical and morphological characteristics was tested on the VT23M alloy. The test results showed that the method meets the quality requirements for the automated diagnostics of fracture mechanisms of titanium alloys.

Validation of Selected Non-Destructive Methods for Determining the Compressive Strength of Masonry Units Made of Autoclaved Aerated Concrete.

Minor-destructive (MDT) and non-destructive (NDT) techniques are not commonly used for masonry as they are complex and difficult to perform. This paper describes validation of the following methods: semi-non-destructive, non-destructive, and ultrasonic technique for autoclaved aerated concrete (AAC). The subject of this study covers the compressive strength of AAC test elements with declared various density classes of: 400, 500, 600, and 700 (kg/m³), at various moisture levels. Empirical data including the shape and size of specimens, were established from tests on 494 cylindrical and cuboid specimens, and standard cube specimens 100 mm × 100 mm × 100 mm using the general relationship for ordinary concrete (Neville's curve). The effect of moisture on AAC was taken into account while determining the strength fBw for 127 standard specimens tested at different levels of water content (w = 100%, 67%, 33%, 23%, and 10%). Defined empirical relations were suitable to correct the compressive strength of dry specimens. For 91 specimens 100 mm × 100 mm × 100 mm, the P-wave velocity cp was tested with the transmission method using the ultrasonic pulse velocity method with exponential transducers. The curve (fBw⁻cp) for determining the compressive strength of AAC elements with any moisture level (fBw) was established. The developed methods turned out to be statistically significant and can be successfully applied during in-situ tests. Semi-non-destructive testing can be used independently, whereas the non-destructive technique can be only applied when the developed curve fbw⁻cp is scaled.

3D Printed Templating of Extrinsic Freeze-Casting for Macro-Microporous Biomaterials.

As with most biological materials, natural bone has hierarchical structure. The microstructural features of compact bone are of various length scales with its porosity consisting of larger osteons (∼100 µm diameter) and vascular channels, as well as smaller lacuna spaces (∼10 µm diameter). In this study, the freeze-casting process, which has been previously used to form biocompatible porous scaffolds (made with hydroxyapatite, HA) has been improved to mimic the intrinsic hierarchical structure of natural bone by implementing an extrinsic 3D printed template. The results of pore characterization showed that this novel combined method of 3D printing and freeze-casting is able to produce porosity at multiple length scales. Nonporous, microporous (created with freeze-casting alone), and macro-micro porous (created with freeze-casting and 3D printed templating) scaffolds were compared as substrates to evaluate cellular activities using osteoblast-like MG63 cell lines. The number of cells oriented parallel to the HA wall structures in the freeze-cast scaffold was found to increase on the microporous and macro-micro porous samples when compare to the nonporous samples, mimicking the natural alignment of the lamella of natural bone. Regarding the cell morphologies, cells on microporous and macro-micro porous samples showed narrowly aligned shapes, whereas those on nonporous samples had polygonal shapes with no discernible orientation. Proliferation and differentiation tests demonstrated that no toxicity or functional abnormalities were found in any of the substrates due to potential chemical and mechanical residues that may have been introduced by the freeze-casting process. Monitoring of the three-dimensional distribution of cells in the scaffolds through microcomputed tomography indicates that the cells were well distributed in the interior pore spaces via the interpenetrating macro-micro pore networks. In summary, we demonstrate this novel approach can create porosity at multiple length scales and is highly favorable in creating a biocompatible, osteoconductive, and structurally hierarchical HA scaffolds for biomedical applications.

Modeling age-specific facial development in Williams-Beuren-, Noonan-, and 22q11.2 deletion syndromes in cohorts of Czech patients aged 3-18 years: A cross-sectional three-dimensional geometric morphometry analysis of their facial gestalt.

Three-dimensional (3D) virtual facial models facilitate genotype-phenotype correlations and diagnostics in clinical dysmorphology. Within cross-sectional analysis of both genders we evaluated facial features in representative cohorts of Czech patients with Williams-Beuren-(WBS; 12 cases), Noonan-(NS; 14), and 22q11.2 deletion syndromes (22q11.2DS; 20) and compared their age-related developmental trajectories to 21 age, sex and ethnically matched controls in 3-18 years of age. Using geometric morphometry statistically significant differences in facial morphology were found in all cases compared to controls. The dysmorphic features observed in WBS were specific and manifested in majority of cases. During ontogenesis, dysmorphic features associated with increased facial convexity become more pronounced whereas other typical features remained relatively stable. Dysmorphic features observed in NS cases were mostly apparent during childhood and gradually diminished with age. Facial development had a similar progress as in controls, while there has been increased growth of patients' nose and chin in adulthood. Facial characteristics observed in 22q11.2DS, except for hypoplastic alae nasi, did not correspond with the standard description of its facial phenotype because of marked facial heterogeneity of this clinical entity. Because of the sensitivity of 3D facial morphometry we were able to reach statistical significance even in smaller retrospective patient cohorts, which proves its clinical utility within the routine setting.

Ag0 Nanoparticles Synthesized in R-phycoerythrin: Change in Bioconjugate Properties upon Ripening of Nanoparticles.

BACKGROUND: Bioconjugates prepared from photoactive molecules and metal nanoparticles are suitable for the development of new optoelectronic devices and for theranostic applications in medicine. METHODS: We produced a bioconjugate of R-phycoerythrin (R-PE), a photosynthetic pigment of red algae, with Ag0 nanoparticles (Ag0 NPs) synthesized in its tunnel cavities by mixing aqueous solutions of AgNO3 and R-PE without exogenous reductant. In freshly prepared bioconjugate (Ag0⋅R-PE), the diameter of Ag0 NPs measured in electron microscopic images was 6.5 ± 0.5 nm, being commensurate with the length of R-PE tunnel cavity, and they were found to emit bright visible fluorescence. RESULT: The initial aggregation temperature (T0) of freshly prepared Ag0⋅R-PE was decreased, compared to that of native R-PE (33 vs. 44°C). Upon the ripening of Ag0 NPs, their fluorescence was almost entirely quenched, and giant surface-enhanced Raman scattering was recorded. The bioconjugate self-assembled into nanorods 25 ± 5 nm long and could withstand heating to 90°C. Further heating (90°C → 100°C) resulted in the formation of filaments which are 0.5 ± 0.2 µm long and spherical aggregates 60 ± 10 nm in diameter. CONCLUSION: These data can contribute to the development of practical applications for the Ag0⋅R-PE bioconjugate.

Stem Cells, Patterning and Regeneration in Planarians: Self-Organization at the Organismal Scale.

The establishment of size and shape remains a fundamental challenge in biological research that planarian flatworms uniquely epitomize. Planarians can regenerate complete and perfectly proportioned animals from tiny and arbitrarily shaped tissue pieces; they continuously renew all organismal cell types from abundant pluripotent stem cells, yet maintain shape and anatomy in the face of constant turnover; they grow when feeding and literally degrow when starving, while scaling form and function over as much as a 40-fold range in body length or an 800-fold change in total cell numbers. This review provides a broad overview of the current understanding of the planarian stem cell system, the mechanisms that pattern the planarian body plan and how the interplay between patterning signals and cell fate choices orchestrates regeneration. What emerges is a conceptual framework for the maintenance and regeneration of the planarian body plan on basis of the interplay between pluripotent stem cells and self-organizing patterns and further, the general utility of planarians as model system for the mechanistic basis of size and shape.

An Automated Two-dimensional Pairwise form Registration Method for Pair-matching of Fragmented Skeletal Remains.

This study introduces an automated pairwise method for osteological pair-matching of fragmented skeletal remains using two-dimensional fragmented outlines extracted from photographs. The form data are used in pairwise iterative closest point registrations with rigid transformations. A modified version of the average Hausdorff distance is calculated to remove any coordinate correspondences with outline fracture margins, which allow the distance analysis of fragmented outlines. A dilation modification to the Hausdorff distance is proposed creating a greater separation between true- and false-pairs. The sample consists of 122 calcanei (61 pairs) from the UI-Stanford collection. Performance statistics are provided for simulated fragmented and complete assemblages. Results indicate up to 98% accuracy for fragmented and complete assemblages. The dilated Hausdorff distance performed similarly across assemblages, but showed a slight decrease in performance for the complete assemblage. This approach provides a useful short listing tool to reduce the number of visual comparisons required in large commingled assemblages.