Spectrographic Vocal Characteristics in Female Teachers: Finger Kazoo Intensive Short-term Vocal Therapy.

OBJECTIVE: Verify the results from intensive short-term vocal therapy using the Finger Kazoo technique about the spectrographic vocal measurements of teachers. METHODS: Controlled and randomized trial. Spectrographic vocal assessment was performed by judges before and after intensive short-term vocal therapy with Finger Kazoo. Sample was composed of 41 female teachers. There were two study groups (with vocal nodules and without structural affection of the vocal folds) and the respective control groups. For the statistical analysis of the data, nonparametric tests were used (Mann-Whitney test and Wilcoxon test). RESULTS: After intensive short-term vocal therapy with Finger Kazoo, improvement in voice spectral parameters, such as improvement in tracing (color intensity and regularity), greater definition of formants and harmonics, increased replacement of harmonics by noise, and a greater number of harmonics, mainly in the group without structural affection of the vocal folds. CONCLUSION: There was an improvement in the spectrographic vocal parameters, showing greater stability, quality, and projection of the emission, especially in female teachers without structural affection of the vocal folds.

Spectrographic Voice Analysis Protocol (SAP): Convergent, Concurrent, and Accuracy Validity.

OBJECTIVE: To verify the convergent and concurrent validity of the Spectrographic Voice Analysis Protocol (SAP) and its accuracy to discriminate dysphonic from nondysphonic patients. METHOD: The study used 82 vowel /Ɛ/ samples and their respective narrowband spectrograms, analyzed with SAP. Cepstral peak prominence (CPP) and cepstral peak prominence smoothed (CPPS) verified the convergent validity of the SAP total score, while the general grade of vocal deviation (GG) verified the concurrent validity of the SAP total score. The ROC (receive operator curve) curve and its accuracy, sensitivity, and specificity values, positive predictive value (PPV) and negative predictive value (NPV), and positive likelihood ratio (LR+) and negative likelihood ratio (LR-) verified the accuracy of the SAP score to discriminate dysphonic from nondysphonic individuals. RESULTS: Dysphonic and nondysphonic had different SAP total scores. In the convergent validity, the SAP score had a weak and moderate negative correlation, respectively, with CPP and CPPS, as well as a moderate positive correlation with GG. SAP performed well in discriminating dysphonic from nondysphonic individuals (area under the curve = 82.0%; sensitivity = 91.7%; specificity = 51.7%; PPV = 93.7%; NPV = 44.0%; LR+ = 6.21; LR- = 0.53) based on the 8-point cutoff score. CONCLUSION: SAP has convergent validity with CPP and CPPS and concurrent validity with GG. The SAP total score performed well in discriminating dysphonic from nondysphonic individuals. However, the specificity, NPV, and LR- values justify cautiously using SAP, always in combination with other information in clinical voice assessment.

Diffusion tensor imaging magnetic resonance imaging (DTI-MRI) helps to tailor speech therapy: A case report with a short narrative review.

BACKGROUND: Stroke has functional sequelae, including motor weakness, spasticity, dysphagia, and neurogenic bladder deteriorating activities of daily living. Speech therapy is more often an essential part of the rehabilitation program. Studies in aphasia have primarily focused on two major pathways: the arcuate fasciculus and the superior longitudinal fasciculus. The arcuate fasciculus is a major fiber bundle connecting Broca's area (associated with language production) and Wernicke's area (associated with language comprehension). Damage to this pathway can result in different types of aphasia, depending on the location and extent of the injury. CASE DESCRIPTION: Tractography is a neuroimaging technique used to map the white matter tracts in the brain. Our patient had an occlusion of the external carotid and left carotid siphon. The patient exhibited early complex language deficits involving both motor expressive and comprehensive abilities. After three months the patient presented conduction aphasia caused by damage to the inferior parietal lobule, which extends into the subcortical white matter and damages the arcuate fascicle. This patient was re-assessed for spasticity and language treatments needs, few months after the stroke. Considering first two months language improvement followed by any further language expressive improvement from 3rd to 6th month, a diffusion tensor imaging (DTI) tractography was requested to study interconnections between cortical and subcortical matter. The brain magnetic resonance imaging (MRI) performed in our hospital showed an extensive malacic gliotic area in the left temporal-frontal parietal site. CONCLUSION: Brain MRI is confirmed as a multimodal tool evaluating the damage, both from the point of structural and functional view. Tractography in aphasia allows focusing on major pathways. The involvement of the arcuate fascicle, whose lesion disconnects Broca's and Wernicke's areas, is related to clinical improvement, and represents a neural correlate of the brain injury recovery process that physicians and speech therapists might be aware of it, tailoring the plane of care of each patient.

Campo dinâmico vocal: avaliação da funcionalidade da voz / Dynamic vocal analysis: vocal functionality evaluation

RESUMO O campo dinâmico vocal (CDV) é uma estratégia de avaliação vocal perceptivo-auditiva e acústica que oferece estimativas sobre a biomecânica e a aerodinâmica da produção vocal por meio da realização de tarefas de variação de frequência e de intensidade e do uso da espectrografia acústica da voz. Esse relato de experiência tem como objetivo demonstrar a aplicação do CDV na avaliação da funcionalidade vocal de indivíduos disfônicos e não-disfônicos, com foco especial na musculatura laríngea. As tarefas fonatórias envolvem emissão de vogal sustentada e/ou de fala encadeada em três intensidades autosselecionadas (habitual autorreferida, fraca e forte) e em três frequências (habitual autorreferida, aguda, grave), além do glissando. As tarefas de variação de intensidade e de frequência possibilitam a avaliação da musculatura laríngea no controle da adução glótica e do alongamento e encurtamento das pregas vocais. A tarefa de variação de frequência permite também a análise da musculatura extrínseca no controle da posição vertical da laringe no pescoço. Enquanto a vogal sustentada avalia a funcionalidade vocal com foco na laringe, a fala encadeada permite a avaliação dos ajustes articulatórios empregados. A aplicação do CDV será demonstrada por meio de pranchas espectrográficas de indivíduos normais e disfônicos. Indivíduos vocalmente saudáveis realizam as tarefas do CDV de forma equilibrada, com qualidade vocal adequada e sem esforço fonatório, denotando boa funcionalidade vocal. Por outro lado, indivíduos com dificuldade na realização das tarefas do CDV, com piora da qualidade vocal e/ou com aumento da tensão muscular, podem apresentar funcionalidade vocal alterada.

ABSTRACT Dynamic vocal analysis (DVA) is an auditory-perceptual and acoustic vocal assessment strategy that provides estimates on the biomechanics and aerodynamics of vocal production by performing frequency and intensity variation tasks and using voice acoustic spectrography. The objective of this experience report is to demonstrate the use of DVA in the assessment of vocal functionality of dysphonic and non-dysphonic individuals, with a special focus on the laryngeal musculature. Phonatory tasks consisted of sustained vowel, "a" or "é", and/or connected speech, in three intensities (habitual, soft, and loud) and three frequencies (habitual, high, and low), as well as ascending and descending glissando. The adjustments of the laryngeal and paralaryngeal muscles can be inferred from the different DVA tasks. The main characteristics of the laryngeal muscles analyzed are control of glottic adduction, stretching, and shortening of the vocal folds; the main characteristics of the paralaryngeal musculature are mainly related to the vertical laryngeal position in the neck. While the sustained vowel evaluates the vocal functionality with a focus on the larynx, connected speech allows the evaluation of the articulatory adjustments employed. An acoustic spectrographic software can be used to visualize the performance of such tasks. The clinical application of the DVA will be exemplified using acoustic spectrography plates from normal and dysphonic voices, taken from a voice bank. Individuals who perform the DVA tasks in a balanced way, with adequate vocal quality and without phonatory effort, demonstrate good vocal functionality. On the other hand, difficulties in performing these tasks with worsening vocal quality and/or increased muscle tension may be indications of altered vocal functionality.

Add-On MEmaNtine to Dopamine Antagonism to Improve Negative Symptoms at First Psychosis- the AMEND Trial Protocol.

Background: Antipsychotic drugs are primarily efficacious in treating positive symptoms by blocking the dopamine D2 receptor, but they fail to substantially improve negative symptoms and cognitive deficits. The limited efficacy may be attributed to the fact that the pathophysiology of psychosis involves multiple neurotransmitter systems. In patients with chronic schizophrenia, memantine, a non-competitive glutamatergic NMDA receptor antagonist, shows promise for ameliorating negative symptoms and improving cognition. Yet, it is unknown how memantine modulates glutamate levels, and memantine has not been investigated in patients with first-episode psychosis. Aims: This investigator-initiated double-blinded randomized controlled trial is designed to (1) test the clinical effects on negative symptoms of add-on memantine to antipsychotic medication, and (2) neurobiologically characterize the responders to add-on memantine. Materials and Equipment: Antipsychotic-naïve patients with first-episode psychosis will be randomized to 12 weeks treatment with [amisulpride + memantine] or [amisulpride + placebo]. We aim for a minimum of 18 patients in each treatment arm to complete the trial. Brain mapping will be performed before and after 12 weeks focusing on glutamate and neuromelanin in predefined regions. Regional glutamate levels will be probed with proton magnetic resonance spectroscopy (MRS), while neuromelanin signal will be mapped with neuromelanin-sensitive magnetic resonance imaging (MRI). We will also perform structural and diffusion weighted, whole-brain MRI. MRS and MRI will be performed at an ultra-high field strength (7 Tesla). Alongside, participants undergo clinical and neuropsychological assessments. Twenty matched healthy controls will undergo similar baseline- and 12-week examinations, but without receiving treatment. Outcome Measures: The primary endpoint is negative symptom severity. Secondary outcomes comprise: (i) clinical endpoints related to cognition, psychotic symptoms, side effects, and (ii) neurobiological endpoints related to regional glutamate- and neuromelanin levels, and structural brain changes. Anticipated Results: We hypothesize that add-on memantine to amisulpride will be superior to amisulpride monotherapy in reducing negative symptoms, and that this effect will correlate with thalamic glutamate levels. Moreover, we anticipate that add-on memantine will restore regional white matter integrity and improve cognitive functioning. Perspectives: By combining two licensed, off-patent drugs, AMEND aims to optimize treatment of psychosis while investigating the memantine response. Alongside, AMEND will provide neurobiological insights to effects of dual receptor modulation, which may enable future stratification of patients with first-episode psychosis before initial antipsychotic treatment. Clinical Trial Registration: [ClinicalTrials.gov], identifier [NCT04789915].

Objective and subjective evaluation of Velopharyngeal Dysfunction (VPD) following surgical repair of the cleft palate using the furlow palatoplasty - A new tool.

Velopharyngeal dysfunction (VPD) diagnosis and speech surgery outcomes are currently based solely on subjective evaluation criteria consisting of perceptual speech assessment and functional imaging. This study describes an objective and comparable method in VPD assessment and investigates the concurrence between the objective and subjective evaluations. The present study included 20 paediatric patients presenting with VPD after primary repair (intravelar veloplasty) of cleft palate. Our protocol was based on computerized analysis of voice parameters by means of an objective tool, spectrography integrated with Multi-Dimensional Voice Program (MDVP). The protocol also included perceptual evaluation by speech therapist and phoniatrician (consensus listening), and parents. This is a single surgeon, single centre experience and all patients underwent a secondary Furlow's palatoplasty. Assessments were performed pre- and postoperatively and upon completion of speech therapy. Results were compared using the two-tailed t student test for paired data. Statistical significance was set for p-values <0.05. Data analysis confirmed an improvement in velopharyngeal closure after surgery and speech therapy consistently with the results of perceptual evaluations. The results of the study confirmed the availability and reliability of an objective method for VPD evaluation based on the analysis of voice parameters with investigations that are simple and easily available in a hospital setting.

Plant-Based Natural Bioactive Compounds 2,4-Ditert-Butylphenolas: A Potential Candidates Against SARS-Cov-2019.

The novel coronavirus 2019 is spreading around the world and causing serious concern. However, there is limited information about novel coronavirus that hinders the design of effective drug. Bioactive compounds are rich source of chemo preventive ingredients. In our present research focuses on identifying and recognizing bioactive chemicals in Lantana camara, by evaluating their potential toward new coronaviruses and confirming the findings using molecular docking, ADMET, network analysis and dynamics investigations.. The spike protein receptor binding domain were docked with 25 identified compounds and 2,4-Ditertbutyl-phenol (-6.3kcal/mol) shows highest docking score, its interactions enhances the increase in binding and helps to identify the biological activity. The ADME/toxicity result shows that all the tested compounds can serve as inhibitors of the enzymes CYP1A2 and CYP2D6. In addition, Molecular dynamics simulations studies with reference inhibitors were carried out to test the stability. This study identifies the possible active molecules against the receptor binding domain of spike protein, which can be further exploited for the treatment of novel coronavirus 2019. The results of the toxicity risk for phytocompounds and their active derivatives showed a moderate to good drug score.

Interrater reliability of spectrogram for detecting wheezing in children.

BACKGROUND: Auscultation is an easy way to evaluate and diagnose patients with lung conditions but has the shortcoming of being subjective. Using the spectrogram, it is possible to visualize wheezing. We therefore conducted a study to compare the efficacy of diagnosing wheezing by auscultation versus diagnosing wheezing by spectrogram. METHODS: This was an investigation of interrater reliability and agreement in which the subject population consisted of children, and the rater population consisted of pediatric pulmonologists. We recorded 55 respiratory sound files from June to November 2019. Three pediatric pulmonologists listened to the respiratory sound files and assessed whether wheezing was present. All respiratory sound files were also converted into spectrograms; the same pulmonologists viewed these and assessed whether wheezing was present. We tested for interrater reliability and agreement between the auscultation results and spectrographic results and investigated the diagnostic reliability of auscultation versus spectrogram. RESULTS: Agreement among the three raters of our auscultation respiratory recordings was 88% and reliability was good (κ = 0.76, P < 0.001). Agreement among the three raters of our spectrograms was 83% and reliability was good (κ =0.66, P < 0.001). The level of agreement between each rater's spectrographic findings and diagnosed wheezing was 91%, 75%, and 93%, respectively. Reliability was accordingly very good, moderate, and very good (κ = 0.82, 0.49, 0.85, P < 0.001), respectively. CONCLUSIONS: A spectrogram may be a valuable tool for evaluating wheezing in children. It may also be used to improve a young clinician's ability to accurately diagnose wheezing in the future.

Shedding light on 19th century spectra by analyzing Lippmann photography.

From uncovering the structure of the atom to the nature of the universe, spectral measurements have helped some of science's greatest discoveries. While pointwise spectral measurements date back to Newton, it is commonly thought that hyperspectral images originated in the 1970s. However, the first hyperspectral images are over a century old and are locked in the safes of a handful of museums. These hidden treasures are examples of the first color photographs and earned their inventor, Gabriel Lippmann, the 1908 Nobel Prize in Physics. Since the original work of Lippmann, the process has been predominately understood from the monochromatic perspective, with analogies drawn to Bragg gratings, and the polychromatic case treated as a simple extension. As a consequence, there are misconceptions about the invertibility of the Lippmann process. We show that the multispectral image reflected from a Lippmann plate contains distortions that are not explained by current models. We describe these distortions by directly modeling the process for general spectra and devise an algorithm to recover the original spectra. This results in a complete analysis of the Lippmann process. Finally, we demonstrate the accuracy of our recovery algorithm on self-made Lippmann plates, for which the acquisition setup is fully understood. However, we show that, in the case of historical plates, there are too many unknowns to reliably recover 19th century spectra of natural scenes.

Spectrographic Vocal Assessment Protocol (SVAP): Reliability and Criterion Validity.

OBJECTIVE: To present evidence of the reliability and validity in the Spectrographic Vocal Assessment Protocol. METHODS: The sample consisted of 50 female participants, aged 25-62 years old, 24 with a otorhinolaryngological diagnosis of the normal larynx or posterior glottal gap, and 26 diagnosed with vocal fold disturbances. The emission of the vowel /a:/ was analyzed by the Real Time Spectrogram software. The spectrographs were analyzed with a protocol by two speech therapist judges. For reliability analysis, inter- and intrajudge analysis were performed using the Kendall coefficient. For criterion validity, the t test for matched samples with Bonferroni correction was used to compare the average performance of the protocol between the two groups of subjects. RESULTS: Protocol reliability: for broadband items, the agreement between judges ranged from moderate (0.462) to almost perfect (0.888), and it is significant for all items, and an intrajudge agreement was almost perfect and significant (0.865-1.000) for all items. For narrowband items, the agreement between judges ranged from moderate (0.469) to almost perfect (0.922), and it is significant for all items except for the presence of "subharmonics in high frequencies" where agreement was moderate (0.504), but not significant, and the intrajudge agreement was almost perfect (0.874-1.000) and significant for all items. Criterion validity: for most broadband and narrowband items, the groups with normal or altered diagnosis differed statistically. CONCLUSION: The Spectrographic Vocal Assessment Protocol presented adequate evidence in reliability and validity.

Development and Evidence of Content Validity of the Spectrographic Vocal Assessment Protocol (SVAP).

OBJECTIVE: To develop and seek evidence of content validity of a spectrographic vocal assessment protocol. METHODS: Methodological study for development and validation of an assessment instrument. A broad literature search was conducted to develop and substantiate a spectrographic vocal assessment protocol. The protocol items were sent to five expert judges, speech therapists, nonauthors of the research, doctors, and clinicians, with experience in the field of voice and spectrographic acoustic analysis, who individually analyzed the protocol items. For data analysis, the Gwet analysis and the Content Validity Ratio formula were used. RESULTS: The Spectrographic Vocal Assessment Protocol was developed, subdivided into Broadband and Narrowband. Twenty-four protocol items had Content Validity Ratio 1.0 and 25 items had 0.6. Gwet analysis indicated substantial agreement (0.656) for broadband and almost perfect (0.848) for narrowband items. CONCLUSION: Evidence of satisfactory content validity was obtained in the development of the protocol.

Ausculta cervical en el paciente pediátrico con parálisis cerebral Revision narrativa / Cervical auscultation in pediatric patient with cerebral palsy Narrative review

Dentro de la atención clínica, el profesional de la fonoaudiología cuenta con recursos evaluativos para describir la biomecánica secuencial de la ingesta; uno de los recursos utilizados es la Auscultación Cervical (AC). (Dudik et al., 2016) Teniendo en cuenta la descripción de la importancia de la intervención oportuna y efectiva de los procesos de alimentación en los niños con parálisis cerebral, se considera necesario resolver aspectos relevantes alrededor de la relación de las características clínicas de estos niños en su proceso de deglución y los registros del perfil espectrográfico en su fase faríngea de la deglución a partir de la aplicación de la AC. La organización de la revisión establece la búsqueda de artículos científicos en la utilización de la AC en la evaluación clínica de la deglución en sujetos pediátricos y diagnosticados con Parálisis Cerebral. La información encontrada muestra cómo en ausencia de procesos instrumentales de evaluación deglutoria, la AC aporta como beneficio la accesibilidad del procedimiento, además de características de temporalidad en la secuencia de los sonidos deglutorio, sin ningún tipo de factor adverso para su realización. Es por ello que la auscultación es un recurso importante en la evaluación clínica y se puede establecer como un primer paso en el análisis de los aspectos fisiológicos de la fase faríngea de la deglución, pone a la auscultación en una posición privilegiada, pero que debe ser estandarizada por los estudiosos y teóricos del tema.

In the clinical care, the speech language pathologist has evaluative resources to describe the biomechanics of swallowing; one of the resources used is Cervical Auscultation (CA). (Dudik et al., 2016) Taking into account the description of the importance of the timely and effective intervention of the swallowing processes in children with cerebral palsy, it is considered necessary to solve relevant aspects around the relationship of the clinical characteristics of these children in their swallowing process and the records of the spectrographic profile in their pharyngeal phase of swallowing from the application of CA. In the absence of instrumental swallowing evaluation processes, CA provides the accessibility of the procedure as a benefit, in addition to temporality characteristics in the sequence of swallowing sounds, without any type of adverse factor for its performance. The CA is an important resource in clinical evaluation, it establishes a first step in the physiological analysis of swallowing with the physiological aspects of the pharyngeal phase. This puts auscultation in a privileged position, but it must be standardized by cientifics and theorists on the subject.

Virtual protein quantification laboratory enhancing online teaching.

Virtual laboratory simulations can contribute to the educational objectives related to practical classes, especially in situations of online or hybrid teaching. We present a proposal for laboratory activity involving the quantification of proteins with the biuret reaction and measurement by visible light spectrometry. The student will be able to develop the procedures and obtain the absorbance values for calculations and discussions using the ProtVirt software, integrating its use with the teacher's pedagogical proposal.

Analysis of oral-nasal balance after intensive speech therapy combined with speech bulb in speakers with cleft palate and hypernasality.

OBJECTIVE: To evaluate the efficacy of the combination of a speech bulb with an intensive speech therapy program in hypernasal participants with cleft palate. METHODS: Twenty hypernasal speakers with cleft palate (12 females and 8 males, median age 28.45 years), who were wearing speech bulbs underwent an intensive speech therapy program of 45 sessions over 3 weeks. Three experienced speech-language pathologists rated the participants' speech recordings before and after intensive speech therapy, with and without the speech bulb. Nasometric recordings and long-term averaged spectra were also analyzed using repeated-measures ANOVAs. RESULTS: The ANOVA of the hypernasality ratings showed significant effects of therapy [F (1,19) = 15.97; p < .001], speech bulb [F(1,190 = 28.54, p < .001] and a therapy -speech bulb interaction effect [F(1.19) = 22.30, p < 0.001]. The most favorable listener ratings of hypernasality were obtained post-therapy when participants were wearing their speech bulbs. Without the speech bulb, intensive speech therapy by itself did not result in a significant improvement. With speech bulb, nasalance scores for high [F (1,19) = 14.07, p < .001] and low pressure [F (1,19) = 14.84, p < .001] sentences were significantly lower post-therapy, providing preliminary evidence that an intensive speech therapy program may enhance the effect of a speech bulb. Before and after comparisons of individual nasalance profiles demonstrated variable improvement in 15 participants, no progress in 2 participants and more severe hypernasality after therapy in 3 participants. Long-term averaged spectra corroborated the findings of the perceptual analysis. Based on a frequency bin from 201 to 300 Hz, there was a significant within-subject effect for with and without speech bulb [F(1, 18) = 4.54, p = .047] as well as for before vs. after session [F (1,18) = 7.14, p = .015]. CONCLUSION: The speech bulb in combination with intensive speech therapy resulted in improved oral-nasal balance for the majority of participants. More research is needed to investigate long-term outcomes as well as individual factors contributing to therapy success.

Classificação espectrográfica do sinal vocal: relação com o diagnóstico laríngeo e a análise perceptivo-auditiva / Spectrographic classification of the vocal signal: relation with laryngeal diagnosis and auditory-perceptual analysis

RESUMO Objetivo Verificar se existe associação entre a presença de alteração laríngea, a análise perceptivo-auditiva da qualidade vocal e a classificação espectrográfica do sinal vocal em indivíduos com distúrbio de voz. Métodos Participaram 478 pacientes com distúrbios de voz. Foi realizada gravação da vogal /Ɛ/ sustentada e o exame médico para estabelecimento de diagnóstico laríngeo. Os espectrogramas da vogal foram utilizados para classificação dos sinais em Tipo I, II, III e IV. Resultados Vozes de indivíduos sem alteração laríngea foram classificadas, predominantemente, como Tipo I e Tipo II, enquanto sinais de indivíduos com alteração laríngea foram classificados nos Tipos III e IV. Vozes desviadas foram classificadas, predominantemente, como Tipo II, enquanto os sinais de pacientes com desvio vocal foram categorizados, predominantemente, como Tipos II e III. Apenas os sinais de indivíduos com desvio vocal foram classificados como Tipo IV. Sinais Tipo III e IV apresentaram valores mais elevados no grau geral do desvio e nos graus de rugosidade e soprosidade, em relação aos sinais Tipo I e Tipo II. Os sinais Tipo IV apresentaram maior grau geral e graus de rugosidade e soprosidade, em comparação aos sinais Tipo III. Apenas os sinais Tipo IV apresentaram valores mais elevados no grau de tensão, em relação aos sinais Tipo I, II e III. Conclusão Há associação entre a presença de alteração laríngea, a análise perceptivo-auditiva e a classificação espectrográfica do sinal vocal em indivíduos com distúrbio de voz.

ABSTRACT Purpose To verify whether there is an association between the presence of laryngeal alteration, auditory-perceptual analysis of vocal quality, and the spectrographic classification of the vocal signal in individuals with voice disorders. Methods 478 patients with voice disorders participated in the study. A recording of the sustained vowel /Ɛ/ and a medical examination were performed to establish a laryngeal diagnosis. The vowel spectrograms were used to classify the signals into type I, II, III and IV. Results Voices of individuals without laryngeal disorders were predominantly classified as type I and type II, while signals of individuals with laryngeal disorders were classified as types III and IV. Deviated voices were predominantly classified as type II, while the signals of patients with vocal deviation were predominantly categorized as types II and III. Only the signals of individuals with vocal deviation were classified as type IV. Type III and IV signals showed higher values for the general degree of deviation and for degrees of roughness and breathiness in relation to type I and type II signals. Type IV signals showed a higher general degree and degrees of roughness and breathiness compared to type III signals. Only type IV signals showed higher values in the degree of tension compared to types I, II and III signals. Conclusion There is an association between the presence of laryngeal alteration, auditory-perceptual analysis, and the spectrographic classification of the vocal signal in individuals with voice disorders.

Advances in High-Field MRI.

MRI techniques and systems have evolved dramatically over recent years. These advances include higher field strengths, new techniques, faster gradients, improved coil technology, and more robust sequence protocols. This article reviews the most commonly used advanced MRI techniques, including diffusion-weighted imaging, magnetic resonance spectrography, diffusion tensor imaging, and cerebrospinal fluid flow tracking.

VDAC1 Mediated Anticancer Activity of Gallic Acid in Human Lung Adenocarcinoma A549 Cells.

AIMS: Gallic acid (GA) is generally distributed in a variety of plants and foods, and possesses cell growth-inhibiting activities in cancer cell lines. In the present study, the impact of GA on cell viability, apoptosis induction and possible molecular mechanisms in cultured A549 lung carcinoma cells was investigated. METHODS: In vitro experiments showed that treating A549 cells with various concentrations of GA inhibited cell viability and induced apoptosis in a dose-dependent manner. In order to understand the mechanism by which GA inhibits cell viability, comparative proteomic analysis was applied. The changed proteins were identified by Western blot and siRNA methods. RESULTS: Two-dimensional electrophoresis revealed changes that occurred to the cells when treated with or without GA. Four up-regulated protein spots were clearly identified as malate dehydrogenase (MDH), voltagedependent, anion-selective channel protein 1(VDAC1), calreticulin (CRT) and brain acid soluble protein 1(BASP1). VDAC1 in A549 cells was reconfirmed by western blot. Transfection with VDAC1 siRNA significantly increased cell viability after the treatment of GA. Further investigation showed that GA down regulated PI3K/Akt signaling pathways. These data strongly suggest that up-regulation of VDAC1 by GA may play an important role in GA-induced, inhibitory effects on A549 cell viability.

Content evidence of a spectrographic analysis protocol / Evidência de conteúdo de um protocolo de análise espectrográfica

ABSTRACT Objective: to verify the content evidence of a spectrographic analysis protocol. Methods: a methodological study in which five speech therapists who assessed the clarity and the relevance of the protocol were selected. The Content Validity Index (CVI) was used to investigate the level of agreement among judges regarding overall aspects, items and domains of the protocol. Results: most judges considered the overall aspects of the protocol as comprehensive. As for clarity, 17 items showed an excellent content validity (CVI ≥ 0.78), three showed a good content validity (0.60 ≤ CVI ≤ 0.77) and two items were judged as poor (CVI ≤ 0.59). As for relevance, 19 items obtained an excellent content validity (CVI ≥ 0.78) and three had a good content validity (0.60 ≤ CVI ≤ 0.77). The judges suggested adding items related to vocal signal normality in all domains. After the analysis, 18 items required no reformulation, five items were added, three were reformulated and one was excluded. Conclusion: the proposed protocol was regarded as a comprehensive one. The items presented a good to excellent content validity as for clarity and relevance. After this validation step, the protocol ended up presenting 25 items distributed into five domains.

RESUMO Objetivo: verificar a evidência de conteúdo de um protocolo de análise espectrográfica. Métodos: trata-se de um estudo metodológico. Foram selecionados cinco fonoaudiólogos que avaliaram a clareza e relevância do protocolo. Utilizou-se o Índice de Validade de Conteúdo (IVC) para investigar o nível de concordância entre os juízes quanto ao aspecto global, itens e domínios do protocolo. Resultados: a maioria dos juízes considerou o aspecto global do protocolo abrangente. Quanto à clareza, 17 itens demonstraram validade de conteúdo excelente (IVC≥ 0,78), três obtiveram boa validade de conteúdo (0,60 ≤ IVC≤ 0,77) e dois itens foram julgados como ruins (IVC≤ 0,59). Com relação à relevância, 19 itens obtiveram excelente validade de conteúdo (IVC≥ 0,78) e três foram avaliados com boa validade de conteúdo (0,60 ≤ IVC≤ 0,77). Os juízes sugeriram a inserção de itens relativos à normalidade do sinal vocal em todos os domínios. Após análise, 18 itens mantiveram-se sem reformulação, cinco itens foram inseridos, três itens foram reformulados e um item foi excluído. Conclusão: o protocolo proposto foi considerado abrangente. Os itens apresentaram validade de conteúdo de boa a excelente quanto à clareza e relevância. Após essa etapa de validação, o protocolo passou a apresentar 25 itens, distribuídos entre os cinco domínios.

Randomized, Prospective Double-Blinded Study Comparing 3 Different Doses of 5-Aminolevulinic Acid for Fluorescence-Guided Resections of Malignant Gliomas.

BACKGROUND: Five-aminolevulinic acid (5-ALA) is used for fluorescence-guided resections of malignant glioma at a dose of 20 mg/kg; yet, it is unknown whether lower doses may also provide efficacy. OBJECTIVE: To perform a double-blinded randomized study comparing 3 different doses of 5-ALA. METHODS: Twenty-one patients with suspected malignant glioma were randomly assigned to 0.2, 2, or 20 mg/kg 5-ALA. Investigators were unaware of dose. Intraoperatively, regions of interest were first defined in tumor core, margin, and adjacent white matter under white light. Under violet-blue illumination, the surgeon's impression of fluorescence was recorded per region, followed by spectrometry and biopsy. Plasma was collected after administration and analyzed for 5-ALA and protoporphyrin IX (PPIX) content. RESULTS: The positive predictive value of fluorescence was 100%. Visual and spectrometric fluorescence assessment showed 20 mg/kg to elicit the strongest fluorescence in tumor core and margins, which correlated with cell density. Spectrometric and visual fluorescence correlated significantly. A 10-fold increase in 5-ALA dose (2-20 mg/kg) resulted in a 4-fold increase of fluorescence contrast between marginal tumor and adjacent brain. t max for 5-ALA was 0.94 h for 20 mg/kg (0.2 kg: 0.50 h, 2 mg/kg: 0.61 h). Integrated PPIX plasma levels were 255.8 and 779.9 mcg*h/l (2 vs 20 mg/kg). Peak plasma concentrations were observed at 1.89 ± 0.71 and 7.83 ± 0.68 h (2 vs 20 mg/kg; average ± Standard Error of Mean [SEM]). CONCLUSION: The highest visible and measurable fluorescence was yielded by 20 mg/kg. No fluorescence was elicited at 0.2 mg/kg. Increasing 5-ALA doses did not result in proportional increases in tissue fluorescence or PPIX accumulation in plasma, indicating that doses higher than 20 mg/kg will not elicit useful increases in fluorescence.

Detectable voice change with the edrophonium test in laryngeal myasthenia gravis.

A case of laryngeal myasthenia gravis in a 65-year-old woman presenting with hoarseness as the sole symptom is reported. Voice spectrography was performed before and after injection of intravenous edrophonium. There was a marked improvement in the patient's voice after the administration of edrophonium, which was confirmed by the changes seen on the sound spectrogram. This was the only objective indication of a diagnosis of myasthenia gravis. No thymoma was seen on chest X-ray and the patient was negative for anti-acetylcholine receptor antibodies. Treatment for laryngeal myasthenia gravis was initiated and the patient's vocal problems resolved. This case emphasizes the need to consider systemic diseases in the differential diagnosis of hoarseness and demonstrates the need for careful follow-up in such patients.