ABSTRACT
OBJECTIVE: to investigate CD133 immunoexpression, cancer stem cells marker, in oral epithelial dysplasias (OEDs) and oral squamous cells carcinomas (OSCCs) and understandits possible involvement in the malignant transformation process of these lesions and to better elucidate their biological behavior. MATERIAL AND METHODS: Tissue samples of 15 cases of OSCCs and 15 OEDs were subjected to CD133 antibody immunohistochemistry reactions. The analysis used quantitative parameters (number of immunostained cells regardless of immunostaining sublocations). RESULTS: All samples of OSCCs and OEDs showed positive immunostaining, with no significant difference between these groups (p = 0.283). We did not observe statistical difference between the degree of dysplasia and the amount of CD133+ cells (p = 0.899). CD133 immunoexpression showed no association with the OEDs and OSCCs sites. It was observed that nuclear and cytoplasmic immunostaining was more evident with the progression of the malignant process. CONCLUSION: It is suggested that the CD133 cellular localization together with the histopathological criteria of OEDs classification can contribute to provide more concrete indications about the oral carcinogenesis process.
Subject(s)
AC133 Antigen/metabolism , Biomarkers, Tumor/metabolism , Carcinoma, Squamous Cell/pathology , Focal Epithelial Hyperplasia/pathology , Mouth Neoplasms/pathology , Precancerous Conditions/pathology , Adult , Aged , Aged, 80 and over , Carcinoma, Squamous Cell/metabolism , Female , Focal Epithelial Hyperplasia/metabolism , Follow-Up Studies , Humans , Male , Middle Aged , Mouth Neoplasms/metabolism , Precancerous Conditions/metabolism , PrognosisABSTRACT
O carcinoma de células escamosas é uma neoplasia maligna comum em cães e gatos, e sua ocorrência está relacionada a diversos fatores, porém a exposição constante a luz ultravioleta, ausência de pigmento na epiderme, e a perda ou pouca cobertura de pelos estão entre as principais causas da ocorrência de tal neoplasia. Os sinais clínicos característicos desta neoplasia são lesões ulcerativas, com focos de necrose, hemorragia e região adjacentes avermelhadas. O tratamento consiste da excisão cirúrgica ou criocirurgia dependendo da extensão da lesão, radioterapia e quimioterapia adjuvante. O presente estudo objetiva relatar um caso de utilização de flape em padrão axial para reparar defeito após ressecção de carcinoma de células escamosas em região abdominal de cão, destacando o sucesso de seu tratamento e a manobra cirúrgica como parte importante no resultado final.AU
Squamous cells carcinoma is a common malignancy in dogs and cats, and its occurrence is related tomany factors, but the constant exposure to ultraviolet light, absence of pigment in the epidermis, and the loss or little coverage by hair are the main cause of occurrence of this malignancy. The characteristic clinical signs of this malignancy are ulcerative lesions with foci of necrosis, hemorrhage and reddish adjacent region. The treatment consists of excision or cryosurgery depending on the extent of the injury, radiation therapy and adjuvant chemotherapy. This study aims to report the use of pattern axial flap to repair de fect after resection of squamous cells carcinoma in the abdominal region of dog, as well as highlighting the success of treatment, and surgical procedure as an important part in the final result.
Subject(s)
Animals , Dogs , Carcinoma, Squamous Cell/therapy , Carcinoma, Squamous Cell/veterinary , Necrosis , NeoplasmsABSTRACT
O carcinoma de células escamosas é uma neoplasia maligna comum em cães e gatos, e sua ocorrência está relacionada a diversos fatores, porém a exposição constante a luz ultravioleta, ausência de pigmento na epiderme, e a perda ou pouca cobertura de pelos estão entre as principais causas da ocorrência de tal neoplasia. Os sinais clínicos característicos desta neoplasia são lesões ulcerativas, com focos de necrose, hemorragia e região adjacentes avermelhadas. O tratamento consiste da excisão cirúrgica ou criocirurgia dependendo da extensão da lesão, radioterapia e quimioterapia adjuvante. O presente estudo objetiva relatar um caso de utilização de flape em padrão axial para reparar defeito após ressecção de carcinoma de células escamosas em região abdominal de cão, destacando o sucesso de seu tratamento e a manobra cirúrgica como parte importante no resultado final.
Squamous cells carcinoma is a common malignancy in dogs and cats, and its occurrence is related tomany factors, but the constant exposure to ultraviolet light, absence of pigment in the epidermis, and the loss or little coverage by hair are the main cause of occurrence of this malignancy. The characteristic clinical signs of this malignancy are ulcerative lesions with foci of necrosis, hemorrhage and reddish adjacent region. The treatment consists of excision or cryosurgery depending on the extent of the injury, radiation therapy and adjuvant chemotherapy. This study aims to report the use of pattern axial flap to repair de fect after resection of squamous cells carcinoma in the abdominal region of dog, as well as highlighting the success of treatment, and surgical procedure as an important part in the final result.
Subject(s)
Animals , Dogs , Carcinoma, Squamous Cell/therapy , Carcinoma, Squamous Cell/veterinary , Necrosis , NeoplasmsABSTRACT
Se presentan 5 casos clínicos de pacientes atendidas en la consulta de patología de cuello del Hospital Provincial Ginecoobstétrico "Mariana Grajales Coello" de Santiago de Cuba por presentar sangrado vaginal, dolor en bajo vientre, leucorrea y lesiones vaginales. El estudio anatomopatológico confirmó el diagnóstico de carcinoma de células escamosas o epidermoide.
Five case reports of patients who were assisted at the cervix Pathology Department from "Mariana Grajales Coello" Provincial Gynecological Obstetrical Hospital in Santiago de Cuba due to vaginal bleeding, low abdominal pain, leukorrhea and vaginal injuries are presented. The pathological study confirmed the diagnosis of squamous or epidermoid cells carcinoma.
ABSTRACT
Introduction : Lip squamous cell carcinoma is a malignant lesion of aggressive behavior, which must be recognized by health professionals to prevent damage to patient's health.Objective:To present incisional biopsy importance as an effective clinical approach for the diagnosis of lip squamous cell carcinoma and actinic cheilitis malignancy as well as the professional's lack of knowledge on these two diseases. Case report:The patient was under medical treatment due to be positive for human immunodeficiency virus and because of the actinic cheilitis on lower lip. Because the patient did not observe the labial lesion regression, he searched for the School of Dentistry of the University Center of the Educational Foundation of Barretos, where he underwent biopsy. This procedure confirmed the diagnosis of lip squamous cell carcinoma. The patient was referred for treatment in a specialized cancer center and has been living with the cancer consequences. Conclusion:The physician and dentist must be aware of the main clinical features of lip squamous cell carcinoma so that they can establish its correct diagnosis and early treatment.
ABSTRACT
O câncer bucal é uma doença que afeta principalmente homens acima de 40 anos com hábito de fumar e beber. Em geral, esses pacientes têm o diagnóstico da enfermidade realizado tardiamente, acarretando tratamentos complexos, mutilantes e de alto custo tanto financeiro como social e de prognóstico sombrio a ponto do câncer bucal apresentar uma taxa de sobrevida em 5 anos inferior a 50%. O diagnóstico precoce representa uma alternativa importante para alterar esse panorama. Os recursos de que os profissionais da área da saúde, principalmente os cirurgiões-dentistas, dispõem são o diagnóstico clínico de lesões estabelecidas acompanhado da biópsia com exame histopatológico.Outra alternativa para o diagnóstico precoce dos carcinomas espinocelulares ocorre quando estes são precedidos de lesões cancerizáveis. Nas duas últimas décadas, a utilização da citopatologia como método de diagnóstico de danos celulares prévios ao aparecimento de lesões clínicas possibilitou sua utilização em mucosa bucal. Os estudos apresentados tratam especificamente desta aplicação clínica, abordando: a) os aspectos genéticos do câncer bucal; b) a correlação histocitopatológica de lesões cancerizáveis em câncer bucal e na mucosa normal exposta aos carcinógenos; c) a validação das amostras citopatológicas; d) a padronização da técnica de extração de DNA em células esfoliadas da mucosa bucal...
Oral cancer affects mainly men over 40 years who are exposed to tobacco and alcohol. These patients usually receive a late diagnosis, which results in complex, mutilating treatments with high financial and social costs, poor prognosis, and, therefore, a 5-year survival rate lower than 50%. Early diagnosis is critical to change this situation. The resources currently available to healthcare professionals, particularly dentists, are the clinical diagnosis of lesions and biopsies for histopathologic examination. Another alternative for the early diagnosis of squamous cell carcinomas is the identification of precursor lesions. In the last two decades, cytopathology has been used as a method to diagnose cell damage that precedes the appearance of clinical lesions, and this use can be extended to the oral mucosa. The studies reported here deal specifically with this clinical application, and discuss: A) the genetic factors in oral cancer; B) the cytohistologic correlation of cancer precursor lesions and the normal mucosa exposed to carcinogens; C) the validation of cytopathologic samples; D) the standardization of the technique to extract DNA of cells exfoliated from the oral mucosa...
Subject(s)
Humans , Male , Alcoholism , Carcinoma, Squamous Cell , Leukoplakia, Oral , Mouth Neoplasms , Pathology, Oral , Smoking , Risk FactorsABSTRACT
A six-year old Simmental cow was referred to the Veterinary Hospital in Cuiaba, Brazil, with an ocular tumor involving the cornea and conjunctiva. The animal was submitted to regional anesthesia after which superficial keratectomy and conjunctival incision were performed. Third palpebral flap and adequate post-surgical therapy were employed. By the 12th post operative day the fluorescein dye test was negative. The tumor was examined by histopathology and was diagnosed as ocular squamous cells carcinoma. Relapse was not observed up to 18 months after the surgery.
Foi encaminhado ao Hospital Veterinário da Faculdade de Medicina Veterinária da Universidade de Cuiabá um bovino com seis anos de idade, fêmea, da raça Simental, apresentando neoformação de aspecto granular no canto nasal superior da córnea e da conjuntiva. Realizaram-se ceratectomia superficial e incisão conjuntival para exérese da massa tumoral, seguido de "flap" de terceira pálpebra e terapêutica clínica apropriada. Decorridos 12 dias de pós-operatório observou-se teste de fluoresceína negativo. Pela histopatologia do tecido extraído firmou-se o diagnóstico de carcinoma de células escamosas. Até 18 meses de pós-operatório não se observou recidiva.