ABSTRACT
Stalk rots are important biotic constraints to sorghum production worldwide. Several pathogens may be associated with the disease, but Macrophomina phaseolina and Fusarium thapsinum are recognized as the major causal organisms. The diseases become more aggressive when drought and high-temperature stress occur during grain filling. Progress in genetic improvement efforts has been slow due to lack of effective phenotyping protocol and the strong environmental effect on disease incidence and severity. Deployment of modern molecular tools is expected to accelerate efforts to develop resistant hybrids. This study was aimed at identifying genomic regions associated with resistance to both causal organisms. A sorghum diversity panel consisting of 300 genotypes assembled from different parts of the world was evaluated for response to infection by both pathogens. Community resources of 79,132 single nucleotide polymorphic (SNP) markers developed on the panel were used in association studies using a multi-locus mixed model to map loci associated with stalk rot resistance. Adequate genetic variation was observed for resistance to both pathogens. Structure analysis grouped the genotypes into five subpopulations primarily based on the racial category of the genotypes. Fourteen loci and a set of candidate genes appear to be involved in connected functions controlling plant defense response. However, each associated SNP had relatively small effect on the traits, accounting for 19-30% of phenotypic variation. Linkage disequilibrium analyses suggest that significant SNPs are genetically independent. Estimation of frequencies of associated alleles revealed that durra and caudatum subpopulations were enriched for resistant alleles, but the results suggest complex molecular mechanisms underlying resistance to both pathogens.
Subject(s)
Disease Resistance/genetics , Genome-Wide Association Study , Plant Diseases/genetics , Plant Diseases/microbiology , Sorghum/genetics , Sorghum/microbiology , Alleles , Ascomycota/physiology , Chromosomes, Plant/genetics , Fusarium/physiology , Gene Frequency/genetics , Genotype , Linkage Disequilibrium/genetics , Phenotype , Plant Diseases/immunology , Polymorphism, Single Nucleotide/genetics , Reproducibility of Results , Sorghum/immunologyABSTRACT
No Brasil, diversas espécies de Fusarium são patogênicas ao milho, afetando sementes, plântulas, colmos e grãos. Objetivou-se comparar isolados de F. verticillioides e de duas espécies do complexo F. graminearum (F. graminearum sensu stricto e F. meridionale), de ocorrência predominante em sementes de milho, quanto à taxa de crescimento micelial, capacidade de colonização e redução da germinação da semente, redução da emergência de plântulas e infecção e colonização de colmos de milho.Testes permutacionais e análise de contraste foram usados para discriminar os isolados. Análise de componentes principais foi utilizada para identificar as variáveis responsáveis pela maior variância entre os isolados. Com exceção da redução da germinação, os isolados F. verticillioides apresentaram menores valores de taxa de crescimento micelial, eficiência de infecção, redução na emergência de plântulas e comprimento de lesão em colmos, quando comparados aos demais, que não se diferenciaram. As variáveis redução da emergência e redução da germinação apresentaram maior influência na caracterização dos isolados, sugerindo que sementes infectadas com isolados de qualquer uma das espécies do complexo F. graminearum testadas representam maior risco ao estabelecimento da cultura do que isolados de F. verticillioides.
In Brazil, several Fusarium species are pathogenic to corn affecting seeds, seedlings, stalks and grains. This research aimed to compare isolates from F. verticillioides and from two species of the F. graminearum complex (F. graminearum sensu stricto and F. meridionale) prevalent on corn seeds in relation to mycelial growth rate, ability to colonize and reduce seed germination, reduction in seedling emergence and infection and colonization of corn stalk. Permutational test and contrast analysis was performed to discriminate the isolates and species. Principal component analysis was used to identify the variables leading to greatest variance among the isolates. With the exception of seed germination, F. verticillioides isolates showed lower values for mycelial growth rate, infection efficiency, reduction in seedling emergence and lesion size than F. graminearum complex isolates that were all similar. Seedling emergence and seed germination showed greater influence on the characterization of isolates, suggesting that seeds infected with F. graminearum isolates of any of the species tested represent a greater threat to crop stand than F. verticillioides isolates.
