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1.
Zhen Ci Yan Jiu ; 49(5): 472-479, 2024 May 25.
Article in English, Chinese | MEDLINE | ID: mdl-38764118

ABSTRACT

OBJECTIVES: To investigate the effect of Peitu Yimu(strengthening spleen and soothing liver) acupuncture on intestinal mucosal barrier function and corticotropin-releasing factor (CRF)/CRF receptor 1 (CRFR1) pathway in rats with diarrhea-predominant irritable bowel syndrome (IBS-D), so as to explore its underlying mechanism in alleviating IBS-D. METHODS: Forty female SD rats were randomly divided into blank, model, electroacupuncture (EA), and agonist groups, with 10 rats in each group. Except for the blank group, rats in the other groups were given folium sennae infusion by gavage combined with chronic unpredictable mild stress to establish IBS-D model. Rats in the EA group received acupuncture at "Tianshu"(ST25) and EA at "Zusanli"(ST36) and "Taichong"(LR3) (2 Hz/15 Hz) on one side for 20 min, with the side chosen alternately every other day, for 14 days after modeling. Rats in the agonist group received acupuncture 30 min after intravenous injection of CRFR1 agonist urocortin, with the same manipulation method and time as the EA group. Before and after intervention, visceral pain threshold and stool Bristol scores were measured. Elevated plus maze test and open field test were used to detect anxiety and depression like behavior of rats. ELISA was used to detect the contents of CRF and CRFR1 in rats serum. Immunohistochemistry was used to detect the positive expressions of CRF, CRFR1, zonula occludens protein 1(ZO-1), occlusal protein(Occludin), and closure protein 1 (Claudin-1) in colon tissue. RESULTS: Compared with the blank group, the visceral pain threshold, open arm time percentage (OT%), total distance of movement in the open field test, and positive expression of ZO-1, Occludin, and Claudin-1 in colon were decreased (P<0.01, P<0.05), while Bristol stool scores, serum CRF and CRFR1 contents, and positive expressions of CRF and CRFR1 in colon were increased (P<0.01) in the model group. After intervention and compared with the model group, the visceral pain threshold, OT%, total distance of movement in the open field test, and positive expressions of ZO-1, Occludin, and Claudin-1 in colon were increased (P<0.05, P<0.01), while Bristol stool scores, serum CRF and CRFR1 contents, and positive expressions of CRF and CRFR1 in colon were decreased (P<0.01) in the EA group;the Bristol stool scores, serum CRF content, and CRF positive expression in colon were significantly decreased in the agonist group (P<0.01). CONCLUSIONS: Peitu Yimu acupuncture can significantly improve visceral hypersensitivity and anxiety-depression state in IBS-D rats. Its mechanism may be related to the inhibition of CRF/CRFR1 pathway and restoration of intestinal tight junction protein expressions.


Subject(s)
Acupuncture Therapy , Diarrhea , Intestinal Mucosa , Irritable Bowel Syndrome , Receptors, Corticotropin-Releasing Hormone , Animals , Female , Humans , Rats , Acupuncture Points , Claudin-1/metabolism , Claudin-1/genetics , Corticotropin-Releasing Hormone/metabolism , Corticotropin-Releasing Hormone/genetics , Diarrhea/therapy , Diarrhea/metabolism , Diarrhea/genetics , Disease Models, Animal , Intestinal Mucosa/metabolism , Irritable Bowel Syndrome/therapy , Irritable Bowel Syndrome/metabolism , Irritable Bowel Syndrome/genetics , Rats, Sprague-Dawley , Receptors, Corticotropin-Releasing Hormone/metabolism , Receptors, Corticotropin-Releasing Hormone/genetics , Zonula Occludens-1 Protein/metabolism , Zonula Occludens-1 Protein/genetics
2.
Chinese Pharmacological Bulletin ; (12): 181-188, 2024.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-1013613

ABSTRACT

Aim To analyze the differences in plasma biomarkers and metabolic pathways between Atractylodes chinensis and Atractylodes coreana after intervention in spleen deficiency rats, and discuss the spleen strengthening mechanism of the two from a non targeted metabolomics perspective. Methods A spleen deficiency model was established in SD rats using a composite factor method of improper diet, excessive fatigue, and bitter cold diarrhea. To determine the content of gastrointestinal and immunological indicators, UHPLC-QE-MS technology was used, combined with principal component analysis (PC A) and orthogonal projections to latent structures-discriminant analysis (OPLS-DA) methods to search for biomarkers in plasma of spleen deficiency rats, and metabolic pathways were induced using the Pathway database. Results After administration of Atractylodes chinensis and Atractylodes coreana, various indicators in plasma of spleen deficiency rats showed varying degrees of regression. Metabolomics analysis showed that Atractylodes chinensis and Atractylodes coreana respectively recalled 70 and 82 plasma differential metabolites. Atractylodes chinensis mainly regulated two metabolic pathways : "Glycine, serine, and threonine metabolism, and "Thiamine metabolism". Atractylodes coreana mainly regulated five metabolic pathways, "Glycine, serine, and threonine metabolism", "Thiamine metabolism, "Pyrimidine metabolism", "Butanoate metabolism", and "Riboflavin metabolism". Conclusions Both Atractylodes chinensis and Atractylodes coreana have certain regulatory effects on spleen deficiency rats, and their mechanism of action may be related to regulating metabolic pathways such as "Glycine, serine, and threonine metabolism, and "Thiamine metabolism"in spleen deficiency.

3.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-1003830

ABSTRACT

By summarising Professor WANG Xianbo's clinical experience in treating hepatitis B virus associated acute-on-chronic liver failure (HBV-ACLF), it is believed that HBV-ACLF is a syndrome of root-cause deficiency and manifestation excess, with spleen deficiency as the root cause and dampness-heat-toxicity-blood stasis as the manifestation, and the therapeutic methods proposed as “detoxification and cooling of the blood to promote circulation of the internal organs, and strengthening spleen and resolving dampness to take care of the middle energizer”. In the treatment of HBV-ACLF, for syndrome of stasis-heat-toxicity mass, it was common to use the Jiedu Liangxue Formula (解毒凉血方) by detoxifying and cooling blood; For syndrome of dampness-heat-toxicity mass, it was common to use in the Jiedu Liangxue Lishi Formula (解毒凉血利湿方) by detoxifying and cooling the blood, strengthening the spleen and resolving dampness; For syndrome of spleen-deficiency and dampness-heat, it was common to use in the Jiedu Liangxue Jianpi Formula (解毒凉血健脾方, also known as Zhonggan No.2 Formula) by strengthening the spleen and reple-nishing qi, clearing heat and resolving dampness.

4.
China Pharmacy ; (12): 1449-1455, 2023.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-976268

ABSTRACT

OBJECTIVE To provide reference for the clinical application and further research and development of Shenling baizhu powder. METHODS Using the methods of textual research and bibliometrics, the key information such as the composition, dose, processing and usage of Shenling baizhu powder in ancient and modern literature were systematically summarized and sorted out, and the number of publications, keywords, and other contents of modern literature were visually analyzed. RESULTS & CONCLUSIONS Shenling baizhu powder was first collected in Taiping Huimin Hejiju Fang (abbreviated “Jufang”), the composition of core drugs and most widely used dose ratio in previous generations were the same as those in Jufang, basically focusing on powder making and being taken after mixed with liquid; the dosage was concentrated on 2-3 qian; the function and application of it had been expanded to a certain extent compared with Jufang; its clinical application had gradually extended from internal medicine to surgery, gynecology, pediatrics, ophthalmology, dermatology, etc., covering all aspects of the disease caused by spleen deficiency in general; the composition mechanism revolved around “strengthening the spleen, replenishing qi and removing dampness”; the recommended prescription drugs were all selected from the corresponding varieties in the pharmacopoeia. Modern research mainly focuses on clinical practice, with traditional Chinese medicine or traditional Chinese and Western medicine combined with this formula as the main modified treatment for various intestinal diseases, diarrhea diseases, and lung diseases. The efficacy and application are the frontier hot spots today, and the mechanism of action has begun to be explored. The usage in the literature and the inclusion in the pharmacopoeia are inconsistent, and further research is needed.

5.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-905827

ABSTRACT

Objective:To discuss the effect of Sishenwan on phosphatidylinositol-3 kinase/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR) signaling pathway related genes and proteins in colon tissue and interleukin-1<italic>β</italic>(IL-1<italic>β</italic>) and interleukin-10(IL-10) expression levels in serum of ulcerative colitis (UC) model rats with spleen kidney Yang deficiency. Method:The 120 SPF Wistar rats were randomly divided into normal group and model group after 7 days of adaptive feeding in SPF laboratory. The model group were given dinitrobenzene sulfonate (DNBS)/ethanol solution enema+hydrocortisone subcutaneously injection+senna leaf gavage to establish UC model of spleen kidney yang deficiency. The rats who successfully established the model were randomly divided into five groups:model group, mesalazine (0.36 g·kg<sup>-1</sup>) group, and Sishenwan high, medium and low dose (3.2,1.6,0.8 g·kg<sup>-1</sup>) groups, the volume of which was 10 mL·kg<sup>-1</sup>. The model group and the blank group were given distilled water of the same volume. Once a day for 21 days. Observe the general conditions of the rats daily, and record the weight, fecal traits and occult blood of the mice for disease activity index (DAI) scoring.Take the rat colon tissue to observe the gross morphology and colon injury, and score the colon mucosal injury index (CMDI). Hematoxylin-eosin (HE) staining was used to observe pathological changes. Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR)was used to detect the expression level of PI3K, Akt, mTOR mRNA in colon tissue. The levels of IL-1<italic>β</italic> and IL-10 in serum were detected by enzyme-linked immunosorbent assay (ELISA). Western blot was used to detect the expression level of PI3K, phosphorylation (p)-PI3K, Akt, p-Akt, mTOR, p-mTOR protein in colon tissue. Result:Compared with blank group, the general survival status of the rats in model group was relatively poor, the DAI score and the CMDI index were significantly increased (<italic>P<</italic>0.01). The intestinal mucosa partially disappears, the glands disappear, and a large number of inflammatory cells infiltrate and gather in the mucosal layer and the base layer in the pathological sections of the model group. The expression levels of PI3K, Akt, and mTOR mRNA were significantly increased (<italic>P</italic><0.01). The IL-1<italic>β</italic> content was significantly increased and the IL-10 content was significantly decreased (<italic>P</italic><0.01). The expression levels of p-PI3K,p-Akt and p-mTOR protein were significantly increased (<italic>P</italic><0.01). Compared with the model group, the DAI scores of Sishenwan high and medium dose groups and mesalazine group decreased (<italic>P</italic><0.05). The CMDI index of mesalazine group and the high, middle and low dose groups of Sishenwan was significantly reduced (<italic>P</italic><0.01). Pathological sections of rats showed that the inflammatory cells in the drug group decreased, and the mucosal layer structure returned to normal to varying degrees. The mesalazine group and the Sishenwan medium-dose group had the best effects, and the mucosal structure was close to the blank control group. The expression levels of PI3K, Akt, mTOR mRNA in the high and medium dose groups of Sishenwan, mesalazine group and Akt mRNA in low dose group of Sishenwan were significantly reduced (<italic>P</italic><0.05,<italic>P</italic><0.01). The expression levels of PI3K and mTOR mRNA in low-dose group of Sishenwan decreased, but the difference was not statistically significant. The IL-1<italic>β</italic> content was significantly reduced and the IL-10 content was significantly increased in high, medium dose groups of Sishenwan and mesalazine groups (<italic>P</italic><0.05,<italic>P</italic><0.01). The level of IL-1<italic>β</italic> decreased and the level of IL-10 increased in the low-dose group of Sishenwan, but the difference was not statistically significant. The expression levels of p-PI3K, p-Akt and p-mTOR protein in the high, medium, and low dose groups of Sishenwan and mesalazine group decreased to varying degrees, and the differences were statistically significant(<italic>P</italic><0.05,<italic>P</italic><0.01). Conclusion:Sishenwan has the effect of improving the general condition and intestinal mucosal damage of ulcerative colitis model rats with spleen and kidney Yang deficiency. The mechanism may be related to the inhibition of PI3K/Akt/mTOR signaling pathway.

6.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-568157

ABSTRACT

In this article,the professor WANG Li-zhong’s experiences was described,which use the Lipi method to treat the cold,cough,dazzling dizziness,insomnia,stomach pain.Professor WANG Li-zhong Consider that the normal spleen and stomach’s ying and wei are harmonious,and there is no phlegm producted.When the functions of five zang viscera are coordinate,the vital qi is sufficiently and the pathogenic qi can’t injury to the body.Lipi method incarnates the professor WANG Li-zhong’s views to the dieases,its unique to treatment in clinic,and the academic thoughts of treating preventable diseases in Chinese medcine.

7.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-566217

ABSTRACT

Objective:To observe the clinical curative e ect of treating diarrhea-predominant irritable bowel syndrome(D-IBS) with Lichang Tang(Intestine-Regulating Decoction) and sertraline hydrochloride.Methods:72 patients with(D-IBS) was randomly divided into 2 groups:32 cases in the treatment group was given self-prescribed Lichang Tang(modi ed Tongxie Yaofang [Main recipe for painful diarrhea]+Jinlingzi San [Toosendan Powder]) and sertraline hydrochloride(50 mg,p.o.once in the early morning);35 cases in the control group were given Lichang Tang alone for oral administration.During administering these drugs,other drugs were discontinued in the 2 groups.Psychological counseling and dietary modi cation were used in all patients.The course of treatment lasted for 4 weeks.Results:The total e ective rates in the treatment group and the control group were 86.49% and 65.71% respectively,and there was signi cant di erence(P

8.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-524769

ABSTRACT

Objective:To observe clinical therapeutic effect of the method for soothing the liver and strengthening the spleen on fibromyalgia syndrome(FMS).Methods:56 cases of FMS were divided into group A(Primary,n=38)and group B(complicated by other rheumatism,n=18).They were treated by modified Xiaoyao Powder for 12 weeks.Results:The effective rate was 92.11% in group A and 72.22% in group B with significant difference between the two groups(P

9.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-674092

ABSTRACT

Objective:To investigate effects of strengthening the spleen and tonifying the kidney and their combination before and after exercise on relative indexes of metabolism of glucose and amino acids.Methods:Strengthening the spleen alone,tonifying the kidney alone or their combination before and after exercise was given to the rat receiving 7-week gradually increasing load exercise on a running platform,and after quantitative load,contents of serum lactic acid,urea nitrogen,glucose,hepatic glycogen,muscle glycogen,hemoglobin,and malondialdehyde(MDA)in renal tissue and activity of superoxide dismulase(SOD)in the renal tissue were determined.Results:Strengthening the spleen before exercise increased hepatic and muscle glycogen contents,and tonifying the kidney before exercise increased muscle glycogen content,and strengthening the spleen combined with tonifying the kidney before and after exercise increased hemoglobin level,strengthening the spleen followed by tonifying the kidney before exercise decreased serum lactic acid and urea nitrogen levels,increased muscle glycogen content and SOD activity/MDA content ratio in the kidney.Conclusion:Strengthening the spleen and tonifying the kidney play a certain role in reducing motion-related fatigue,while strengthening the spleen followed by tonifying the kidney before exercise has the best results.

10.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-674270

ABSTRACT

Objective To study the effect and mechanism of strengthening the spleen and tonifying the kidney on the function and apoptosis of kidney in rats.Methods The 42 male SD rats were randomly divided into quiet control group,exhausted exercise group and strengthening the spleen and tonifying the kidney group.Twenty four hours after exhaustive exercise,the concentrations of blood urea nitrogen(BUN),serum creatinine and urinary protein were determined.The apoptosis in kidneys of rats was detected by TUNEL.The structure of kidney was observed in the HE stain.Results Comparing with the exhausted exercise group,the concen- trations of BUN,serum creatinine and apoptotic index were lower significantly(P

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