Ultrastructural Changes in the Midgut of Brazilian Native Stingless Bee Melipona scutellaris Exposed to Fungicide Pyraclostrobin.

Melipona scutellaris is a Brazilian stingless bee that is important for pollinating wild flora and agriculture crops. Fungicides have been widely used in agriculture, and floral residues can affect forager bees. The goal of our study was to evaluate the effects of sublethal concentrations of pyraclostrobin on the midgut ultrastructure of M. scutellaris forager workers. The bees were collected from three non-parental colonies and kept under laboratory conditions. The bees were orally exposed continuously for five days to pyraclostrobin in syrup at concentrations of 0.125 ng a.i./µL (FG1) and 0.005 ng a.i./µL (FG2). The control bees (CTL) were fed a no-fungicide sucrose solution, and the acetone solvent control bees (CAC) received a sucrose solution containing acetone. At the end of the exposure, the midguts were sampled, fixed in Karnovsky solution, and routinely processed for transmission electron microscopy. Ultrastructural analysis demonstrated that both the fungicide concentrations altered the midgut, such as cytoplasmic vacuolization (more intense in FG1), the presence of an atypical nuclear morphology, and slightly dilated mitochondrial cristae in the bees from the FG1 and FG2 groups (both more intense in FG1). Additionally, there was an alteration in the ultrastructure of the spherocrystals (FG1), which could be the result of cellular metabolism impairment and the excretion of toxic metabolites in the digestive cells as a response to fungicide exposure. The results indicate that ingested pyraclostrobin induced cytotoxic effects in the midgut of native stingless bees. These cellular ultrastructural responses of the midgut are a prelude to a reduced survival rate, as observed in previous studies.

Harmful Effects of Pyraclostrobin on the Fat Body and Pericardial Cells of Foragers of Africanized Honey Bee.

Managed honey bees are daily exposed in agricultural settings or wild environments to multiple stressors. Currently, fungicide residues are increasingly present in bees' pollen and nectar and can harm colonies' production and survival. Therefore, our study aimed to evaluate the effects of the fungicide pyraclostrobin on the fat body and pericardial cells of Africanized honey bees. The foragers were divided into three experimental treatment groups and two controls: pyraclostrobin 0.125 ng/µL (FG1), 0.025 ng/µL (FG2), 0.005 ng/µL (FG3), untreated control (CTL), and acetone control (CAC). After five days of oral exposure (ad libitum), the bees were dissected and prepared for histopathological and morphometric analysis. The FG1-treated bees showed extensive cytoarchitecture changes in the fat body and pericardial cells, inducing cell death. Bees from the FG2 group showed disarranged oenocytes, peripheral vacuolization, and pyknotic nuclei of pericardial cells, but the cytoarchitecture was not compromised as observed in FG1. Additionally, immune system cells were observed through the fat body in the FG1 group. Bees exposed to FG3 demonstrated only oenocytes vacuolization. A significant decrease in the oenocyte's surface area for bees exposed to all pyraclostrobin concentrations was observed compared to the CTL and CAC groups. The bees from the FG1 and FG2 treatment groups presented a reduced surface area of pericardial cells compared to the controls and the FG3 group. This study highlighted the harmful effects of fungicide pyraclostrobin concentrations at the individual bee cellular level, potentially harming the colony level on continuous exposure.

Sublethal doses of imidacloprid and pyraclostrobin impair fat body of solitary bee Tetrapedia diversipes (Klug, 1810).

Solitary bees present greater species diversity than social bees. However, they are less studied than managed bees, mainly regarding the harmful effects of pesticides present in agroecosystems commonly visited by them. This study aimed to evaluate the effect of residual doses of imidacloprid and pyraclostrobin, alone and in combination, on the fat body (a multifunctional organ) of the neotropical solitary bee Tetrapedia diversipes by means of morphological and histochemical evaluation of oenocytes and trophocytes. Males and females of newly-emerged adults were submitted to bioassays of acute topical exposure. Experimental groups were essayed: control (CTR), solvent control (ACT), imidacloprid (IMI, 0.0028 ng/µL), pyraclostrobin (PYR, 2.7 ng/µL) and imidacloprid + pyraclostrobin (I + P). The data demonstrated that the residual doses applied in T. diversipes adults are sublethal at 96 h. Both oenocytes and trophocytes cells responded to topical exposure to the pesticides, showing morphological changes. In the IMI group, the bee oenocytes showed the greatest proportion of vacuolization and altered nuclei. The pyraclostrobin exposure increased the intensity of PAS-positive labeling (glycogen) in trophocytes. This increase was also observed in the I + P group. Changes in energy reserve (glycogen) of trophocytes indicate a possible mobilization impairment of this neutral polysaccharide to the hemolymph, which can compromise the fitness of exposed individuals. Also, changes in oenocytes can compromise the detoxification function performed by the fat body. This is the first study to show sublethal effects in neotropical solitary bees and highlight the importance of studies with native bees.

Tree age and cultivar-oriented use of mineral oil added to fungicide tank mixture for the control of citrus black spot in sweet orange orchards.

BACKGROUND: Mineral oil added to fungicide spray mixtures has been a frequently used strategy to control citrus black spot (CBS) worldwide. Although mineral oil may increase the efficacy of control, its use represents around 15% of the costs of a CBS spray program. This study aimed to assess the performance of different proportions of mineral oil added to a fungicide tank mixture for CBS control in young (less than 10 years old) and old (more than 12 years old) sweet orange orchards of early ('Hamlin'), mid-season ('Pera') and late-maturing ('Valencia') cultivars in São Paulo state, Brazil. The efficacy of 0.15%, 0.20% or 0.25% mineral oil added to a fungicide spray mixture was determined by assessing CBS incidence, severity and fruit drop in six orchards over two seasons. RESULTS: Fungicide programs with or without oil were effective in reducing 100% CBS symptom expression in both young and old 'Hamlin' orchards and in the young 'Pera' orchard. The lowest mineral oil rate tested (0.15%) showed a reduction in CBS intensity of around 90%, similar to the highest rate tested in the old 'Pera' orchard. The highest cost-benefit program to control CBS in the old 'Valencia' orchard was obtained with the mineral oil rate of 0.25%, commonly used in the São Paulo citrus belt, which reduced CBS severity by up to 97%. CONCLUSION: This study demonstrated that mineral oil rates for CBS control can be adjusted according to tree age and cultivar. These findings contribute to the establishment of more sustainable citrus production by reducing spray costs while maintaining the efficacy of CBS control. © 2021 Society of Chemical Industry.

Do fungicides affect the physiology, reproductive development and productivity of healthy soybean plants?

Fungicides are widely used to control diseases in soybean crops. We hypothesized that fungicides applied to healthy soybean plants compromise the plant's physiology, affect the reproductive process and reduce crop productivity. We aimed to evaluate the photosynthetic process, pollen grain viability and yield components of soybean plants exposed to three commercial fungicides. The experiment was performed twice using soybean cultivar SYN 1378C, disease-free plants, with four treatments: i) control treatment (without any fungicide application); ii) cyproconazole 150 g L-1 + difenoconazole 250 g L-1 (CPZ + DFZ; 250 mL ha-1; without adjuvant); iii) azoxystrobin 300 g Kg-1 + benzovindiflupyr 150 g Kg-1 (AZB + BZP; 200 g ha-1; Nimbus® adjuvant (Syngenta)); and iv) propiconazole 250 g L-1 + difenoconazole 250 g L-1 (PPZ + DFZ; 150 mL ha-1; without adjuvant) in both soybean pre-bloom (V8) and bloom (R1) developmental stages. The experimental design was randomized blocks with four replicates. Phytotoxicity, gas exchange and chlorophyll a fluorescence traits, pollen grain viability, pollen grain germination, flower abortion and soybean production components were evaluated. The fungicides did not affect the physiological traits, pollen grain germination and crop yield.

Aplicação de piraclostrobina em genótipos de milho cultivados em segunda safra / Pyraclostrobin application in different corn genotypes cultivated off season

Objetivou-se com esse estudo avaliar os efeitos fisiológicos, o controle de doenças e os efeitos nos componentes de produção, ocasionados pela aplicação do fungicida piraclostrobina, em diferentes estádios fenológicos de genótipos de milho cultivado em segunda safra, no município de Jataí-GO. Utilizou-se o delineamento de blocos ao acaso, no esquema fatorial 3 x 4, com quatro repetições. Os tratamentos consistiram de três genótipos de milho e quatro épocas de aplicação de piraclostrobina. Não houve interação entre genótipos e aplicações para as variáveis avaliadas. A aplicação de piraclostrobina não interfere na fisiologia, desenvolvimento e produtividade de grãos das plantas de milho cultivados em segunda safra e não houve diferença significativa no controle das doenças ferrugem polissora, mancha de cercospora, mancha de bipolares e mancha branca pela aplicação de piraclostrobina nos genótipos estudados.

Objective with this study evaluate the physiological effects, disease control and effects on the production components of pyraclostrobin fungicide, applied at different phenological stages of of second crop corn genotypes, in Jataí city. In randomized blocks in a factorial design 3 x 4 with four replications. The treatments consisted in three corn genotypes and four periods of pyraclostrobin application. There was no interaction between genotype and applications for the variables evaluated. For the majority of the variables the only difference were between genotypes. The application of pyraclostrobin does not interfere in the physiology, development and grain production of maize plants grown in second crop and there was no significant difference in the control of polissora blight,cercospora spot, bipolaris spot stain and white stain by the application of pyraclostrobin in the genotypes studied.

Aplicação de piraclostrobina em genótipos de milho cultivados em segunda safra / Pyraclostrobin application in different corn genotypes cultivated off season

Objetivou-se com esse estudo avaliar os efeitos fisiológicos, o controle de doenças e os efeitos nos componentes de produção, ocasionados pela aplicação do fungicida piraclostrobina, em diferentes estádios fenológicos de genótipos de milho cultivado em segunda safra, no município de Jataí-GO. Utilizou-se o delineamento de blocos ao acaso, no esquema fatorial 3 x 4, com quatro repetições. Os tratamentos consistiram de três genótipos de milho e quatro épocas de aplicação de piraclostrobina. Não houve interação entre genótipos e aplicações para as variáveis avaliadas. A aplicação de piraclostrobina não interfere na fisiologia, desenvolvimento e produtividade de grãos das plantas de milho cultivados em segunda safra e não houve diferença significativa no controle das doenças ferrugem polissora, mancha de cercospora, mancha de bipolares e mancha branca pela aplicação de piraclostrobina nos genótipos estudados.(AU)

Objective with this study evaluate the physiological effects, disease control and effects on the production components of pyraclostrobin fungicide, applied at different phenological stages of of second crop corn genotypes, in Jataí city. In randomized blocks in a factorial design 3 x 4 with four replications. The treatments consisted in three corn genotypes and four periods of pyraclostrobin application. There was no interaction between genotype and applications for the variables evaluated. For the majority of the variables the only difference were between genotypes. The application of pyraclostrobin does not interfere in the physiology, development and grain production of maize plants grown in second crop and there was no significant difference in the control of polissora blight,cercospora spot, bipolaris spot stain and white stain by the application of pyraclostrobin in the genotypes studied.(AU)

Efficacy of Fungicides Applied for Protectant and Curative Activity Against Myrtle Rust.

Myrtle rust, caused by the pathogen Austropuccinia psidii, affects species of the Myrtaceae, many of which are endemic to Australia and New Zealand. Originating from South America, A. psidii is now present in both countries, necessitating effective chemical control for disease management. Using an artificial inoculation protocol, the efficacy of eight fungicides (tebuconazole/trifloxystrobin, cyproconazole/azoxystrobin, fosetyl aluminum, triforine, triadimenol, oxycarboxin, copper, and tebuconazole) applied as curative or protectant treatments was tested on two native New Zealand species (Lophomyrtus × ralphii and Metrosideros excelsa). The impacts of rate (×2), frequency (single or double), and timing (pre- or postinfection) of fungicide application were investigated. Overall, the most effective fungicides tested across both species were those that included a demethylation inhibitor and strobilurin mix, notably tebuconazole/trifloxystrobin (Scorpio) and cyproconazole/azoxystrobin (Amistar Xtra). These fungicides significantly reduced infection of host plants relative to the water control. Timing of application significantly affected bioefficacy, with applications made 7 days before inoculation or 7 days after inoculation being generally the most effective. The rate of fungicide application was not significant for both host species, with few interaction terms showing overall significance. Key findings from this study will set the foundation for further fungicide bioefficacy research conducted to evaluate formulations and adjuvant mixtures, determine suitable application methods for enhanced retention and coverage, and derive optimum application time for effective protection of native and exotic Myrtaceae species in New Zealand.

Mitochondrial Respiratory Inhibition Promoted by Pyraclostrobin in Fungi is Also Observed in Honey Bees.

There is no use restriction associated with bees for many fungicides used in agriculture; however, this does not always mean that these pesticides are harmless for these nontarget organisms. We investigated whether the fungicide pyraclostrobin, which acts on fungal mitochondria, also negatively affects honey bee mitochondrial bioenergetics. Honey bees were collected from 5 hives and anesthetized at 4 °C. The thoraces were separated, and mitochondria were isolated by grinding, filtering, and differential centrifugation. An aliquot of 0.5 mg of mitochondrial proteins was added to 0.5 mL of a standard reaction medium with 4 mM succinate (complex II substrate) plus 50 nM rotenone (complex I inhibitor), and mitochondrial respiration was measured at 30 °C using a Clark-type oxygen electrode. Mitochondrial membrane potential was determined spectrofluorimetrically using safranin O as a probe, and adenosine triphosphate (ATP) synthesis was determined by chemiluminescence. Pyraclostrobin at 0 to 50 µM was tested on the mitochondrial preparations, with 3 repetitions. Pyraclostrobin inhibited mitochondrial respiration in a dose-dependent manner at concentrations of 10 µM and above, demonstrating typical inhibition of oxidative phosphorylation. Pyraclostrobin also promoted a decline in the mitochondrial membrane potential at doses of 5 µM and above and in ATP synthesis at 15 µM and above. We conclude that pyraclostrobin interferes with honey bee mitochondrial function, which is especially critical for the energy-demanding flight activity of foraging bees. Environ Toxicol Chem 2020;39:1267-1272. © 2020 SETAC.

Kresoxim-methyl and famoxadone as activators of toxigenic potential of Aspergillus carbonarius.

Ochratoxin A (OTA) is a secondary metabolite produced by filamentous fungi species belonging to the genera Penicillium and Aspergillus. The contamination of grapes by ochratoxigenic species occurs worldwide in regions of tropical and temperate climates. Better control of fungal growth is achieved through good cultural practice and proper selection of fungicides. Kresoxim-methyl and famoxadone are the most common fungicides used in vineyards. This study aimed at analysing the OTA production and toxigenic potential of Aspergillus carbonarius under fungicide treatment with famoxadone and kresoxim-methyl. The growth rate of A. carbonarius was evaluated by measuring the glucosamine content and the diameter of the fungal colonies. OTA production was quantified by HPLC analysis. The treatment with fungicides, kresoxim-methyl and famoxadone, significantly reduced the fungal growth, by 76% and 60%, respectively. However, the mycotoxin production was greater in the fungicide-treated groups than the control group, showing that even though the fungicides were effective in controlling fungal growth, they were ineffective against mycotoxin production.

Efficacy of pyraclostrobin on the production and chemical composition of forage and silage of wheat (Triticum aestivum cv. BRS Umbu) and oats (Avena strigosa cv. Embrapa 139) preceded by successive cuts in the hard dough grain stage / Eficácia da piraclostrobina sobre a produção e bromatologia da forragem e da silagem de trigo (Triticum aestivum cv. BRS Umbu) e de aveia (Avena strigosa cv. Embrapa 139) precedidos de cortes sucessivos na fase de grão farináceo

The objective of the experiment was to evaluate the productivity, nutritional value and degradability of forage and silage of wheat (Triticum aestivum cv. BRS Umbu) and black oats (Avena strigosa cv. Embrapa 139), preceded by successive cuts, harvested at the hard dough stage, subjected or not to treatment with pyraclostrobin fungicide. Plants were harvested manually with a height of 0.08 m from the ground surface, at the hard dough stage, after two cuts at the vegetative stage; two applications of pyraclostrobin, 0.6 L ha-1 were made, before the first and the second cut. Pyraclostobin decreased the dry matter losses from 21.1% of the control system to 7.7% for the system with pyraclostrobin, reduced the neutral detergent fiber from 70.05% to 66.73%, decreased detergent fiber acid from 43.37% to 39.96%, decreased lignin from 14.37% to 10.13% and increased the relative value of the food from 75.27% to 82.72%, all in the forage, which resulted in changes in silage, with a decrease in neutral detergent fiber from 64.00% to 58.46%, acid detergent fiber from 38.76% to 35.87%, lignin from 10.11% to 6.78% and an increase in the relative value of the food from 85.87 to 97.26. In the evaluation of the forages, wheat presented the best productivity, with 10,068 kg ha-1 vs. 8,238 kg ha-1 black oats, lower neutral detergent fiber (60.32% vs. 76.46%), lower acid detergent fiber (34.49% vs. 48.84%) and higher relative value of the food (96.04 vs. 61.95), these data were from the forage, which were reflected in the resulting silage. It is recommended to use pyraclostrobin as it reduces losses during fermentation, changes fiber composition and improves the degradability of the food. Among the forages, the wheat was more promising, than black oats, due to its productivity, combined with its better chemical composition and degradability.(AU)

O objetivo do experimento foi avaliar a produtividade, o valor nutricional e a degradabilidade da forragem e da silagem de trigo (Triticum aestivum cv. BRS Umbu) e de aveia preta (Avena strigosa cv. Embrapa 139) precedidos de cortes sucessivos, colhidas em estádio de grão farináceo, submetidos ou não ao tratamento com fungicida piraclostrobina. A colheita das plantas foi realizada de forma manual com altura de 0,08 m da superfície do solo, em estádio de grão farináceo, após dois cortes em estádio vegetativo, realizou-se duas aplicações de piraclostrobina, 0,6 L ha-1, antes do primeiro e do segundo corte. A piraclostobina diminuiu as perdas de matéria seca de 21,1% do sistema controle, para 7,7% para o sistema com piraclostrobina, diminuiu a fibsra em detergente neutro de 70,05% para 66,73%, diminuiu a fibra em detergente ácido de 43,37% para 39,96%, diminuiu a lignina de 14,37% para 10,13% e aumentou o valor relativo do alimento de 75,27% para 82,72%, todos na forragem, que resultou em alterações na silagem, com a diminuição de fibra em detergente neutro de 64,00% para 58,46%, fibra em detergente ácido de 38,76% para 35,87%, lignina de 10,11% para 6,78% e incremento do valor relativo do alimento de 85,87 para 97,26. Na avaliação das forrageiras o trigo apresentou as melhores produtividades, com 10.068 kg ha-1, contra 8.238 kg ha-1 da aveia preta, menor fibra em detergente neutro (60,32% contra 76,46%), menor fibra em detergente ácido (34,49% contra 48,84%) e maior valor relativo do alimento (96,04 contra 61,95), dados estes da forragem, que foram refletidos na silagem resultante. Recomenda-se a utilização da piraclostrobina, pois a mesma diminui perdas durante a fermentação, altera a composição da fibra e melhora a degradabilidade do alimento. Dentre as forrageiras o trigo foi mais promissor, frente a aveia preta, devido a sua produtividade, aliada a sua melhor bromatologia e degradabilidade.(AU)

Efficacy of pyraclostrobin on the production and chemical composition of forage and silage of wheat (Triticum aestivum cv. BRS Umbu) and oats (Avena strigosa cv. Embrapa 139) preceded by successive cuts in the hard dough grain stage / Eficácia da piraclostrobina sobre a produção e bromatologia da forragem e da silagem de trigo (Triticum aestivum cv. BRS Umbu) e de aveia (Avena strigosa cv. Embrapa 139) precedidos de cortes sucessivos na fase de grão farináceo

The objective of the experiment was to evaluate the productivity, nutritional value and degradability of forage and silage of wheat (Triticum aestivum cv. BRS Umbu) and black oats (Avena strigosa cv. Embrapa 139), preceded by successive cuts, harvested at the hard dough stage, subjected or not to treatment with pyraclostrobin fungicide. Plants were harvested manually with a height of 0.08 m from the ground surface, at the hard dough stage, after two cuts at the vegetative stage; two applications of pyraclostrobin, 0.6 L ha-1 were made, before the first and the second cut. Pyraclostobin decreased the dry matter losses from 21.1% of the control system to 7.7% for the system with pyraclostrobin, reduced the neutral detergent fiber from 70.05% to 66.73%, decreased detergent fiber acid from 43.37% to 39.96%, decreased lignin from 14.37% to 10.13% and increased the relative value of the food from 75.27% to 82.72%, all in the forage, which resulted in changes in silage, with a decrease in neutral detergent fiber from 64.00% to 58.46%, acid detergent fiber from 38.76% to 35.87%, lignin from 10.11% to 6.78% and an increase in the relative value of the food from 85.87 to 97.26. In the evaluation of the forages, wheat presented the best productivity, with 10,068 kg ha-1 vs. 8,238 kg ha-1 black oats, lower neutral detergent fiber (60.32% vs. 76.46%), lower acid detergent fiber (34.49% vs. 48.84%) and higher relative value of the food (96.04 vs. 61.95), these data were from the forage, which were reflected in the resulting silage. It is recommended to use pyraclostrobin as it reduces losses during fermentation, changes fiber composition and improves the degradability of the food. Among the forages, the wheat was more promising, than black oats, due to its productivity, combined with its better chemical composition and degradability.

O objetivo do experimento foi avaliar a produtividade, o valor nutricional e a degradabilidade da forragem e da silagem de trigo (Triticum aestivum cv. BRS Umbu) e de aveia preta (Avena strigosa cv. Embrapa 139) precedidos de cortes sucessivos, colhidas em estádio de grão farináceo, submetidos ou não ao tratamento com fungicida piraclostrobina. A colheita das plantas foi realizada de forma manual com altura de 0,08 m da superfície do solo, em estádio de grão farináceo, após dois cortes em estádio vegetativo, realizou-se duas aplicações de piraclostrobina, 0,6 L ha-1, antes do primeiro e do segundo corte. A piraclostobina diminuiu as perdas de matéria seca de 21,1% do sistema controle, para 7,7% para o sistema com piraclostrobina, diminuiu a fibsra em detergente neutro de 70,05% para 66,73%, diminuiu a fibra em detergente ácido de 43,37% para 39,96%, diminuiu a lignina de 14,37% para 10,13% e aumentou o valor relativo do alimento de 75,27% para 82,72%, todos na forragem, que resultou em alterações na silagem, com a diminuição de fibra em detergente neutro de 64,00% para 58,46%, fibra em detergente ácido de 38,76% para 35,87%, lignina de 10,11% para 6,78% e incremento do valor relativo do alimento de 85,87 para 97,26. Na avaliação das forrageiras o trigo apresentou as melhores produtividades, com 10.068 kg ha-1, contra 8.238 kg ha-1 da aveia preta, menor fibra em detergente neutro (60,32% contra 76,46%), menor fibra em detergente ácido (34,49% contra 48,84%) e maior valor relativo do alimento (96,04 contra 61,95), dados estes da forragem, que foram refletidos na silagem resultante. Recomenda-se a utilização da piraclostrobina, pois a mesma diminui perdas durante a fermentação, altera a composição da fibra e melhora a degradabilidade do alimento. Dentre as forrageiras o trigo foi mais promissor, frente a aveia preta, devido a sua produtividade, aliada a sua melhor bromatologia e degradabilidade.

Resposta de híbridos de milho ao uso de piraclostrobina na ausência de doenças / Corn hybrids responses to pyraclostrobin use in disease absences

Objetivando-se avaliar o efeito da aplicação de piraclostrobina em diferentes épocas e combinações de aplicação em dois híbridos simples de milho cultivados na safra de verão, realizou-se um experimento no município de Jataí, Goiás. Adotou-se o delineamento de blocos ao acaso no esquema fatorial 2 x 9 (híbridos x aplicações de piraclostrobina), com 4 repetições. As aplicações foram realizadas em diferentes combinações de fungicidas: presença ou ausência de piraclostrobina + tiofanato metílico + fipronil (100 g i.a.100 kg semente-1) no tratamento de sementes (V0) combinado com a aplicação de piraclostrobina (150 g i.a.ha-1) antes ou após a adubação de cobertura (V4 ou V6) combinado ou não com a aplicação de piraclostrobina + ciproconazole (137,25 g i.a.ha-1) em pré-pendoamento (VT) e uma testemunha (sem uso de piraclostrobina). Foram avaliados: atividade da enzima redutase do nitrato, índice de clorofila, altura de plantas, altura de inserção de espiga, diâmetro de colmo, porcentagem de folhas senescentes, severidade de Puccinia polysora Underw, massa de mil grãos, densidade e produtividade de grãos. Submeteram-se os dados ao teste F para verificação de significância; e para comparação de médias utilizou-se o teste de Tukey, ambos a 5% de probabilidade. Para a maioria dos caracteres avaliados observou-se diferença entre híbridos. Não foi observado efeito das aplicações de piraclostrobina e interação entre os fatores em questão. Conclui-se que não há efeito benéfico ou deletério da aplicação de piraclostrobina em diferentes épocas e combinações de aplicação sobre os dois híbridos simples de milho cultivados na safra de verão.(AU)

An experiment was conducted in Jataí, Goiás, Brazil, aiming to evaluate the effects of the application of pyraclostrobin at different times and with different application combinations in two simple hybrids of corn grown in the summer season. A randomized block design was adopted in a factorial 2 x 9 (hybrid x pyraclostrobinapplications) design, with 4 replications. The applications were performed in different combinations: presence or absence of pyraclostrobin + thiophanate methyl + fipronil (100 g a.i.100 kg seed-1) in seed treatment (V0) combined with pyraclostrobin (150 g a.i.ha-1) application before or after topdressing (V4 or V6) combined or not with pyraclostrobin + cyproconazole (137.25 g a.i.ha-1) application in pre-bolting (VT) and a control (without use of pyraclostrobin). The following evaluations were performed: activity of the nitrate reductase enzyme, chlorophyll index, plant height, corn cob insertion height, stem diameter, percentage of senescent leaves, severity of Puccinia polysora Underw, thousand grain weight, grains density, and grains yield. An F test was performed to analyze the significance, and for a means comparison, a Tukey test was used, both at 5% probability. Differences among hybrids were observed for most of the evaluated traits. There were no effects of the applications of pyraclostrobin and no interaction between the factors in question. It can, therefore, be concluded that there are not beneficial or deleterious effects of the application of pyraclostrobin at different times and with different application combinations in two simple hybrids of corn grown in the summer season.(AU)

Corn hybrids responses to pyraclostrobin use in disease absences / Resposta de híbridos de milho ao uso de piraclostrobina na ausência de doenças

An experiment was conducted in Jataí, Goiás, Brazil, aiming to evaluate the effects of the application of pyraclostrobin at different times and with different application combinations in two simple hybrids of corn grown in the summer season. A randomized block design was adopted in a factorial 2 x 9 (hybrid x pyraclostrobin applications) design, with 4 replications. The applications were performed in different combinations: presence or absence of pyraclostrobin  + thiophanate methyl  + fipronil (100 g a.i.100 kg seed-1) in seed treatment (V0) combined with pyraclostrobin (150  g  a.i.ha-1) application before or after topdressing (V4 or V6) combined or not with pyraclostrobin  + cyproconazole (137.25 g a.i.ha-1) application in prebolting (VT) and a control (without use of pyraclostrobin). The following evaluations were performed: activity of the nitrate reductase enzyme, chlorophyll index, plant height, corn cob insertion height, stem diameter, percentage of senescent leaves, severity of Puccinia polysora Underw, thousand grain weight, grains density, and grains yield. An F test was performed to analyze the significance, and for a means comparison, a Tukey test was used, both at 5% probability. Differences among hybrids were observed for most of the evaluated traits. There were no effects of the applications of pyraclostrobin and no interaction between the factors in question. It  can, therefore, be concluded that there are not beneficial or deleterious effects of the application of pyraclostrobin at different times and with different application combinations in two simple hybrids of corn grown in the summer season.(AU)

Objetivando-se avaliar o efeito da aplicação de piraclostrobina em diferentes épocas e combinações de aplicação em dois híbridos simples de milho cultivados na safra de verão, realizouse um experimento no município de Jataí, Goiás. Adotouse o delineamento de blocos ao acaso no esquema fatorial 2 x 9 (híbridos x aplicações de piraclostrobina), com 4 repetições. As aplicações foram realizadas em diferentes combinações de fungicidas: presença ou ausência de piraclostrobina + tiofanato metílico + fipronil (100 g i.a.100 kg semente-1) no tratamento de sementes (V0) com binado com a aplicação de piraclostrobina (150 g i.a.ha-1) antes ou após a adubação de cobertura (V4 ou V6) combinado ou não com a aplicação de piraclostrobina + ciproconazole (137,25 g i.a.ha-1) em prépendoamento (VT) e uma testemunha (sem uso de piraclostrobina). Foram avaliados: atividade da enzima redutase do nitrato, índice de clorofila, altura de plantas, altura de inserção de espiga, diâmetro de colmo, porcentagem de folhas senescentes, severidade de Puccinia polysora Underw, massa de mil grãos, densidade e produtividade de grãos. Submeteram-se os dados ao teste F para verificação de significância; e para comparação de médias utilizouse o teste de Tukey, ambos a 5% de probabilidade. Para a maioria dos caracteres avaliados observouse diferença entre híbridos. Não foi observado efeito das aplicações de piraclostrobina e interação entre os fatores em questão. Conclui‑se que não há efeito benéfico ou deletério da aplicação de piraclostrobina em diferentes épocas e combinações de aplicação sobre os dois híbridos simples de milho cultivados na safra de verão.(AU)

Corn hybrids responses to pyraclostrobin use in disease absences / Resposta de híbridos de milho ao uso de piraclostrobina na ausência de doenças

An experiment was conducted in Jataí, Goiás, Brazil, aiming to evaluate the effects of the application of pyraclostrobin at different times and with different application combinations in two simple hybrids of corn grown in the summer season. A randomized block design was adopted in a factorial 2 x 9 (hybrid x pyraclostrobin applications) design, with 4 replications. The applications were performed in different combinations: presence or absence of pyraclostrobin  + thiophanate methyl  + fipronil (100 g a.i.100 kg seed-1) in seed treatment (V0) combined with pyraclostrobin (150  g  a.i.ha-1) application before or after topdressing (V4 or V6) combined or not with pyraclostrobin  + cyproconazole (137.25 g a.i.ha-1) application in prebolting (VT) and a control (without use of pyraclostrobin). The following evaluations were performed: activity of the nitrate reductase enzyme, chlorophyll index, plant height, corn cob insertion height, stem diameter, percentage of senescent leaves, severity of Puccinia polysora Underw, thousand grain weight, grains density, and grains yield. An F test was performed to analyze the significance, and for a means comparison, a Tukey test was used, both at 5% probability. Differences among hybrids were observed for most of the evaluated traits. There were no effects of the applications of pyraclostrobin and no interaction between the factors in question. It  can, therefore, be concluded that there are not beneficial or deleterious effects of the application of pyraclostrobin at different times and with different application combinations in two simple hybrids of corn grown in the summer season.

Objetivando-se avaliar o efeito da aplicação de piraclostrobina em diferentes épocas e combinações de aplicação em dois híbridos simples de milho cultivados na safra de verão, realizouse um experimento no município de Jataí, Goiás. Adotouse o delineamento de blocos ao acaso no esquema fatorial 2 x 9 (híbridos x aplicações de piraclostrobina), com 4 repetições. As aplicações foram realizadas em diferentes combinações de fungicidas: presença ou ausência de piraclostrobina + tiofanato metílico + fipronil (100 g i.a.100 kg semente-1) no tratamento de sementes (V0) com binado com a aplicação de piraclostrobina (150 g i.a.ha-1) antes ou após a adubação de cobertura (V4 ou V6) combinado ou não com a aplicação de piraclostrobina + ciproconazole (137,25 g i.a.ha-1) em prépendoamento (VT) e uma testemunha (sem uso de piraclostrobina). Foram avaliados: atividade da enzima redutase do nitrato, índice de clorofila, altura de plantas, altura de inserção de espiga, diâmetro de colmo, porcentagem de folhas senescentes, severidade de Puccinia polysora Underw, massa de mil grãos, densidade e produtividade de grãos. Submeteram-se os dados ao teste F para verificação de significância; e para comparação de médias utilizouse o teste de Tukey, ambos a 5% de probabilidade. Para a maioria dos caracteres avaliados observouse diferença entre híbridos. Não foi observado efeito das aplicações de piraclostrobina e interação entre os fatores em questão. Conclui‑se que não há efeito benéfico ou deletério da aplicação de piraclostrobina em diferentes épocas e combinações de aplicação sobre os dois híbridos simples de milho cultivados na safra de verão.

Análise de crescimento e partição de assimilados em plantas de tomateiro cv. Micro-Tom submetidas ao nitrogênio e piraclostrobina / Growth analysis partitioning of assimilate in tomato plants cv. Micro-Tom submitted to nitrogen and pyraclostrobin

Este trabalho objetivou analisar comparativamente o crescimento e a partição de assimilados em plantas de tomateiro cv. Micro-Tom submetidas ao nitrogênio e piraclostrobina. Esta substância favorece o desenvolvimento de cloroplastos e a síntese de clorofila. Plantas de tomateiro foram submetidas aos tratamentos: T1, solução nutritiva completa sem piraclostrobina; T2, solução nutritiva completa + piraclostrobina; T3, solução nutritiva ½ força de N sem piraclostrobina e T4, solução nutritiva ½ força de N + piraclostrobina. As plantas foram coletadas a intervalos regulares de sete dias após o transplante ao longo do ciclo de cultivo, sendo determinados a massa seca e a área foliar. A partir dos dados primários, foi aplicada a análise de crescimento, sendo calculados a massa seca total (Wt), as taxas instantâneas de produção de matéria seca (Ct), crescimento relativo (Rw) e assimilatória líquida (Ea), a área foliar (Af), as taxas de produção (Ca) e de crescimento relativo de área foliar (Ra), as razões de área foliar (Fa) e de massa foliar (Fw), a área foliar específica (Sa), a partição de matéria seca entre órgãos e o número (Nfr) e massa fresca de frutos (Wfr). Plantas de T1 apresentaram maior Wt, Ct e Wfr em relação àquelas dos demais tratamentos. Entretanto, plantas de T2 apresentaram similar Nfr à plantas de T1, sendo superiores às demais. Além disso, alocaram em relação à matéria seca total e ao final do ciclo, maior porcentagem de matéria seca nos frutos comparativamente a plantas de T3 e T4. A associação entre nitrogênio e piraclostrobina altera o crescimento e a partição de assimilados em plantas de tomateiro cv. Micro-Tom, sendo que àquelas submetidas à ½ dose de nitrogênio apresentam maior massa seca total e menor porcentagem final de matéria seca total em frutos, comparativamente, àquelas submetidas à associação ½ dose de nitrogênio e a piraclostrobina.(AU)

This work aimed at comparing the growth and partitioning of assimilate in tomato plants cv. Micro-Tom subjected to nitrogen and pyraclostrobin. This substance favors the development of chloroplasts and the synthesis of chlorophyll. Tomato plants were submitted to the treatments: T1, complete nutrient solution without pyraclostrobin, T2, complete nutrient solution + pyraclostrobin, T3, ½ strength nutrient solution without N pyraclostrobin and T4, ½ strength nutrient solution N + pyraclostrobin. Plants were collected at regular intervals of seven days after transplantation throughout the crop cycle, with dry mass and leaf area being determined. From the primary data, growth analysis was carried out to calculate total dry matter (Wt), the instantaneous rates of dry matter production (Ct), relative growth (Rw) e net assimilation (Ea), leaf area (Af), production rates (Ca) and relative growth of leaf area index (Ra) and leaf weight (Fw) specific leaf area (Sa) the dry matter partitioning between organs and number (Nfr) and fresh fruit weight (Wfr). Plants of T1 showed higher Wt, Ct and Wfr compared to those of other treatments. However, the T2 plants exhibited similar Nfr to T1 plants, being superior to others. Also allocated on the total dry matter and at the end of the cycle, a higher percentage of dry matter in the seafood compared to T3 and T4 plants. Also they allocated relative to the total dry matter and at the end of the cycle, a higher percentage in fruits of plants to T3 and T4. The association between nitrogen and pyraclostrobin changes the growth and assimilated partition on tomato plants cv. Micro Tom, and those submitted to ½ dose of nitrogen have a higher total dry matter and less final percentage of total dry matter in fruits , comparatively to those submitted to the association ½ dose of nitrogen and pyraclostrobin.(AU)

Acúmulo de carboidratos no desenvolvimento de tomateiro tratado com produtos químicos / Accumulation of carbohydrates in the development of tomato plants treated with different chemical products

Este trabalho teve como objetivo estudar os efeitos fisiológicos da piraclostrobina, boscalida, reguladores vegetais e extrato vegetal no acúmulo de carboidratos durante o desenvolvimento de plantas de tomateiro (Solanum lycopersicum L.), híbrido Giuliana, em condições de ambiente protegido. Os tratamentos estudados foram: T1- testemunha; T2- piraclostrobina 0,2 g L-1; T3- boscalida 0,075 g L-1, T4- piraclostrobina 0,2 g L-1 + boscalida 0,075 g L-1, T5- IBA + GA3 + cinetina 375 mg L-1, T6- GA4+7 + benzilaminopurina 100 mg L-1 e T7- extrato vegetal 100 mg L-1. A curva de acúmulo de carboidratos foi realizada com cinco amostras, em intervalos de 20 dias entre as avaliações, sendo a 1ª avaliação realizada aos 30 dias após o transplantio, no dia da primeira aplicação dos tratamentos. A cada coleta as plantas foram separadas em caule, folha e frutos, nos quais foram avaliados os teores de açúcares solúveis totais, açúcares redutores e sacarose. Também foram avaliados os efeitos dos tratamentos no teor de clorofila e nas trocas gasosas. O delineamento experimental adotado foi inteiramente casualizado, com quatro repetições e seis avaliações destrutivas ao longo do desenvolvimento, sendo uma planta por unidade experimental em cada amostragem. A piraclostrobina e a boscalida aplicados isolados e/ou combinados favoreceram o incremento de carboidratos nas folhas, caules e frutos de...(AU)

This work had the purpose to study the physiological effects of pyraclostrobin, boscalid, plant growth regulators and plant extract on the accumulation of carbohydrates during the development of tomato plants (Solanum lycopersicum L.), hybrid Giuliana, in protected environment conditions. The treatments were: T1- control; T2- pyraclostrobin 0.2 g L-1; T3- boscalid 0.075 g L-1, T4- pyraclostrobin 0.2 g L-1 + boscalid 0.075 g L-1, T5- IBA + GA3 + kinetin 375 mg L-1, T6- GA4+7 + benzylaminopurine 100 mg L-1 and T7- plant extract 100 mg L-1. The carbohydrate accumulation curve was accomplished with 5 samples, at 20-day intervals between evaluations, the 1st evaluation being carried out at 30 days after transplantation, on the day of the first treatment application. At each sampling the plants were separated in stem, leaves and fruits, of which the contents of total soluble sugars, reducing sugars and saccharose were evaluated. The effects of the treatments on chlorophyll content and gas exchanges were also evaluated. The experimental design was completely randomized, with 4 repetitions and 6 destructive evaluations during the development, with 1 plant per experimental unit for each sampling. The pyraclostrobin and boscalid applied in isolation and/or combined favor the increase of carbohydrates in leaves, stems and fruits of tomato hybrid Giuliana.(AU)

Acúmulo de carboidratos no desenvolvimento de tomateiro tratado com produtos químicos / Accumulation of carbohydrates in the development of tomato plants treated with different chemical products

Este trabalho teve como objetivo estudar os efeitos fisiológicos da piraclostrobina, boscalida, reguladores vegetais e extrato vegetal no acúmulo de carboidratos durante o desenvolvimento de plantas de tomateiro (Solanum lycopersicum L.), híbrido Giuliana, em condições de ambiente protegido. Os tratamentos estudados foram: T1- testemunha; T2- piraclostrobina 0,2 g L-1; T3- boscalida 0,075 g L-1, T4- piraclostrobina 0,2 g L-1 + boscalida 0,075 g L-1, T5- IBA + GA3 + cinetina 375 mg L-1, T6- GA4+7 + benzilaminopurina 100 mg L-1 e T7- extrato vegetal 100 mg L-1. A curva de acúmulo de carboidratos foi realizada com cinco amostras, em intervalos de 20 dias entre as avaliações, sendo a 1ª avaliação realizada aos 30 dias após o transplantio, no dia da primeira aplicação dos tratamentos. A cada coleta as plantas foram separadas em caule, folha e frutos, nos quais foram avaliados os teores de açúcares solúveis totais, açúcares redutores e sacarose. Também foram avaliados os efeitos dos tratamentos no teor de clorofila e nas trocas gasosas. O delineamento experimental adotado foi inteiramente casualizado, com quatro repetições e seis avaliações destrutivas ao longo do desenvolvimento, sendo uma planta por unidade experimental em cada amostragem. A piraclostrobina e a boscalida aplicados isolados e/ou combinados favoreceram o incremento de carboidratos nas folhas, caules e frutos de...

This work had the purpose to study the physiological effects of pyraclostrobin, boscalid, plant growth regulators and plant extract on the accumulation of carbohydrates during the development of tomato plants (Solanum lycopersicum L.), hybrid Giuliana, in protected environment conditions. The treatments were: T1- control; T2- pyraclostrobin 0.2 g L-1; T3- boscalid 0.075 g L-1, T4- pyraclostrobin 0.2 g L-1 + boscalid 0.075 g L-1, T5- IBA + GA3 + kinetin 375 mg L-1, T6- GA4+7 + benzylaminopurine 100 mg L-1 and T7- plant extract 100 mg L-1. The carbohydrate accumulation curve was accomplished with 5 samples, at 20-day intervals between evaluations, the 1st evaluation being carried out at 30 days after transplantation, on the day of the first treatment application. At each sampling the plants were separated in stem, leaves and fruits, of which the contents of total soluble sugars, reducing sugars and saccharose were evaluated. The effects of the treatments on chlorophyll content and gas exchanges were also evaluated. The experimental design was completely randomized, with 4 repetitions and 6 destructive evaluations during the development, with 1 plant per experimental unit for each sampling. The pyraclostrobin and boscalid applied in isolation and/or combined favor the increase of carbohydrates in leaves, stems and fruits of tomato hybrid Giuliana.

Análise de crescimento e partição de assimilados em plantas de tomateiro cv. Micro-Tom submetidas ao nitrogênio e piraclostrobina / Growth analysis partitioning of assimilate in tomato plants cv. Micro-Tom submitted to nitrogen and pyraclostrobin

Este trabalho objetivou analisar comparativamente o crescimento e a partição de assimilados em plantas de tomateiro cv. Micro-Tom submetidas ao nitrogênio e piraclostrobina. Esta substância favorece o desenvolvimento de cloroplastos e a síntese de clorofila. Plantas de tomateiro foram submetidas aos tratamentos: T1, solução nutritiva completa sem piraclostrobina; T2, solução nutritiva completa + piraclostrobina; T3, solução nutritiva ½ força de N sem piraclostrobina e T4, solução nutritiva ½ força de N + piraclostrobina. As plantas foram coletadas a intervalos regulares de sete dias após o transplante ao longo do ciclo de cultivo, sendo determinados a massa seca e a área foliar. A partir dos dados primários, foi aplicada a análise de crescimento, sendo calculados a massa seca total (Wt), as taxas instantâneas de produção de matéria seca (Ct), crescimento relativo (Rw) e assimilatória líquida (Ea), a área foliar (Af), as taxas de produção (Ca) e de crescimento relativo de área foliar (Ra), as razões de área foliar (Fa) e de massa foliar (Fw), a área foliar específica (Sa), a partição de matéria seca entre órgãos e o número (Nfr) e massa fresca de frutos (Wfr). Plantas de T1 apresentaram maior Wt, Ct e Wfr em relação àquelas dos demais tratamentos. Entretanto, plantas de T2 apresentaram similar Nfr à plantas de T1, sendo superiores às demais. Além disso, alocaram em relação à matéria seca total e ao final do ciclo, maior porcentagem de matéria seca nos frutos comparativamente a plantas de T3 e T4. A associação entre nitrogênio e piraclostrobina altera o crescimento e a partição de assimilados em plantas de tomateiro cv. Micro-Tom, sendo que àquelas submetidas à ½ dose de nitrogênio apresentam maior massa seca total e menor porcentagem final de matéria seca total em frutos, comparativamente, àquelas submetidas à associação ½ dose de nitrogênio e a piraclostrobina.

This work aimed at comparing the growth and partitioning of assimilate in tomato plants cv. Micro-Tom subjected to nitrogen and pyraclostrobin. This substance favors the development of chloroplasts and the synthesis of chlorophyll. Tomato plants were submitted to the treatments: T1, complete nutrient solution without pyraclostrobin, T2, complete nutrient solution + pyraclostrobin, T3, ½ strength nutrient solution without N pyraclostrobin and T4, ½ strength nutrient solution N + pyraclostrobin. Plants were collected at regular intervals of seven days after transplantation throughout the crop cycle, with dry mass and leaf area being determined. From the primary data, growth analysis was carried out to calculate total dry matter (Wt), the instantaneous rates of dry matter production (Ct), relative growth (Rw) e net assimilation (Ea), leaf area (Af), production rates (Ca) and relative growth of leaf area index (Ra) and leaf weight (Fw) specific leaf area (Sa) the dry matter partitioning between organs and number (Nfr) and fresh fruit weight (Wfr). Plants of T1 showed higher Wt, Ct and Wfr compared to those of other treatments. However, the T2 plants exhibited similar Nfr to T1 plants, being superior to others. Also allocated on the total dry matter and at the end of the cycle, a higher percentage of dry matter in the seafood compared to T3 and T4 plants. Also they allocated relative to the total dry matter and at the end of the cycle, a higher percentage in fruits of plants to T3 and T4. The association between nitrogen and pyraclostrobin changes the growth and assimilated partition on tomato plants cv. Micro – Tom, and those submitted to ½ dose of nitrogen have a higher total dry matter and less final percentage of total dry matter in fruits , comparatively to those submitted to the association ½ dose of nitrogen and pyraclostrobin.

Espectros de gotas e idade de trifólios e sua interação com a área abaixo da curva de progresso da ferrugem da soja / Drops spectra and leaflets age and its interaction with the area under disease progress curve of soybean rust

O objetivo do presente trabalho foi avaliar o efeito de espectros de gotas (grossas, médias e finas) na velocidade de absorção de fungicidas, para trifólios de diferentes idades, através de medida indireta expressa pelo residual de controle de ferrugem asiática da soja (Phakopsora pachyrhizi). O delineamento experimental utilizado foi inteiramente casualizado, com quatro repetições, em arranjo fatorial (3x3x5x4), cujos fatores foram compostos por: três espectros de gotas (grossas, médias e finas); três idades fisiológicas de trifólios na planta (1°, 3° e 5° trifólios); quatro períodos de tempo entre a aplicação de fungicidas e chuva simulada (0, 30, 60 e 120min.), mais uma testemunha sem chuva; três tratamentos com fungicida: azoxistrobina + ciproconazol (60+24g i.a. ha-1) + óleo mineral 0,6L ha-1, azoxistrobina (50g i.a. ha-1) + óleo mineral 0,6L ha-1 e ciproconazol (30g i.a. ha-1), mais uma testemunha sem aplicação. Avaliou-se densidade de gotas por centímetro quadrado, diâmetro mediano volumétrico, diâmetro mediano numérico, amplitude relativa, número de dias para o aparecimento da primeira pústula e a área abaixo da curva de progresso da doença. Verificou-se que gotas de menor DMV proporcionam maior velocidade de absorção de fungicidas. Trifólios mais novos absorvem os fungicidas mais rapidamente. A utilização da mistura de azoxistrobina + ciproconazol proporcionou menor evolução da doença com consequente menor área abaixo da curva de progresso da doença.

This research aimed to evaluate the effect of large, medium and fine droplets spectra and its interaction with the fungicide absorption rate and leaflets age through indirect measurement expressed by the residual of Asian soybean rust (Phakopsora pachyrhizi) control. The experimental design was composed by completely randomized design with four replications in a factorial (3x3x5x4), which factors were composed of: three drops spectra (large, medium and fine); three fisiological leaflets ages setting on the plant (1st, 3rd and 5th leaflets), four time periods between the fungicide application and simulated rain (0, 30, 60 and 120') and a control with no rain; three fungicide treatments with Azoxystrobin+Cyproconazole (60+24g a.i. ha-1) + 0.6L ha-1, Azoxystrobin (50g a.i. ha-1) + 0.6L ha-1 and Cyproconazole (30g a.i. ha-1), and control without spray. It were evaluated the density of droplets per square centimeter, volume median diameter, number median diameter, relative amplitude, number of days for the first pustule display and the area under disease progress curve. It was found that drops with smaller volume median diameter have higher fungicides absorption rates. Newer leaflets provide more quickly fungicides absorption. The use of Azoxystrobin + cyproconazole provided lower disease progress with consequent lower area under the disease progress curve.