ABSTRACT
The fruit of Physalis peruviana is widely used in traditional Colombian medicine as an antidiabetic treatment. The aim of the study reported here was to identify the compounds responsible for the hypoglycemic activity using the α-amylase inhibition test. Bioguided fractionation of a dichloromethane extract of the sticky exudate that covers the fruit allowed the isolation and identification of three new sucrose esters, named as peruvioses C-E (1-3), along with the known peruvioses A (6), B (5) and F (4), the structures of which were elucidated by extensive NMR and MS experiments. These compounds proved to be responsible for the hypoglycemic activity observed in the extract. Peruviose D (2) showed the highest activity, with an inhibitory activity value of 84.8%. This is the first study to establish the potential of sucrose esters as α-amylase inhibitors and to explain the hypoglycemic effect that has traditionally been attributed to gooseberry fruit.
Subject(s)
Enzyme Inhibitors/chemistry , Enzyme Inhibitors/pharmacology , Esters/chemistry , Esters/pharmacology , Fruit/chemistry , Physalis/chemistry , Plant Exudates/chemistry , Plant Exudates/pharmacology , Sucrose/chemistry , alpha-Amylases/antagonists & inhibitors , alpha-Amylases/metabolism , Magnetic Resonance Spectroscopy , Mass SpectrometryABSTRACT
Tomatoes, cultivar Santa Clara, were picked on 10/10/95 and treated with Semperfresh (sucrose esters) at concentrations of 0; 0.5; 1.0; 1.5 and 2,0% (immersion for 1 minute). The fruits were stored for 15 days at 25oC and 70% RH. Fruits treated with Semperfresh presented less colour development and higher firmness than no treated fruits. No effects on weigth loss and soluble solids were observed. The best result was observed with the highest Semperfresh concentration (2%).
Tomates cultivar Santa Clara foram colhidos em 10/10/1995 e tratados com Semperfresh (ésteres de sacarose) a 0; 0,5; 1,0; 1,5 e 2,0%, imersão (1 minuto). Os frutos foram armazenados por 15 dias a 25oC e 70% UR. Os frutos tratados com Semperfresh apresentaram menor desenvolvimento de coloração e menores perdas de firmeza de polpa do que os não tratados. Não houve efeito sobre a perda de peso e os sólidos solúveis totais. Das concentrações utilizadas, o melhor resultado foi obtido com 2% de Semperfresh.
ABSTRACT
Tomatoes, cultivar Santa Clara, were picked on 10/10/95 and treated with Semperfresh (sucrose esters) at concentrations of 0; 0.5; 1.0; 1.5 and 2,0% (immersion for 1 minute). The fruits were stored for 15 days at 25oC and 70% RH. Fruits treated with Semperfresh presented less colour development and higher firmness than no treated fruits. No effects on weigth loss and soluble solids were observed. The best result was observed with the highest Semperfresh concentration (2%).
Tomates cultivar Santa Clara foram colhidos em 10/10/1995 e tratados com Semperfresh (ésteres de sacarose) a 0; 0,5; 1,0; 1,5 e 2,0%, imersão (1 minuto). Os frutos foram armazenados por 15 dias a 25oC e 70% UR. Os frutos tratados com Semperfresh apresentaram menor desenvolvimento de coloração e menores perdas de firmeza de polpa do que os não tratados. Não houve efeito sobre a perda de peso e os sólidos solúveis totais. Das concentrações utilizadas, o melhor resultado foi obtido com 2% de Semperfresh.
ABSTRACT
Aiming to verify the efficiency of TAL-PROLONG (sucrose esters), plums 'Santa Rosa' were stored for 21 days at 0°C and 90-95% RH (+ 3 days of simulated marketing at room temperature). The fruits were immersed in a solution containing 0; 0,5; 1,0; 1,5 and 2,0% of TAL-PROLONG, before storage. During cold storage and marketing, fruits treated with TAL-PROLONG presented less weight loss. The decay and internal breakdown incidence was reduced in fruits treated with TAL-PROLONG and it was efficient for maintening the fruit colour.
Com o objetivo de avaliar o efeito do TAL-PROLONG (ésteres de sacarose), ameixas 'Santa Rosa' foram armazenadas por 21 dias a 0°C e 90-95% UR (+ 3 dias de comercialização simulada em temperatura ambiente). As doses do TAL-PROLONG utilizadas foram 0; 0,5; 1,0; 1,5 e 2,0%, aplicadas antes do armazenamento por imersão das frutas. As frutas tratadas com TAL-PROLONG apresentaram menores perdas de peso durante o armazenamento e comercialização simulada. Houve menor incidência de podridões e desintegração interna de polpa nas frutas tratadas com TAL-PROLONG, o qual foi também eficiente na manutenção da coloração das frutas.
ABSTRACT
Aiming to verify the efficiency of TAL-PROLONG (sucrose esters), plums 'Santa Rosa' were stored for 21 days at 0°C and 90-95% RH (+ 3 days of simulated marketing at room temperature). The fruits were immersed in a solution containing 0; 0,5; 1,0; 1,5 and 2,0% of TAL-PROLONG, before storage. During cold storage and marketing, fruits treated with TAL-PROLONG presented less weight loss. The decay and internal breakdown incidence was reduced in fruits treated with TAL-PROLONG and it was efficient for maintening the fruit colour.
Com o objetivo de avaliar o efeito do TAL-PROLONG (ésteres de sacarose), ameixas 'Santa Rosa' foram armazenadas por 21 dias a 0°C e 90-95% UR (+ 3 dias de comercialização simulada em temperatura ambiente). As doses do TAL-PROLONG utilizadas foram 0; 0,5; 1,0; 1,5 e 2,0%, aplicadas antes do armazenamento por imersão das frutas. As frutas tratadas com TAL-PROLONG apresentaram menores perdas de peso durante o armazenamento e comercialização simulada. Houve menor incidência de podridões e desintegração interna de polpa nas frutas tratadas com TAL-PROLONG, o qual foi também eficiente na manutenção da coloração das frutas.