Multidrug-resistant Escherichia coli strains isolated from swine manure biofertilizer in Brazil.

Escherichia coli is one of the key bacteria responsible for a variety of diseases in humans and livestock-associated infections around the globe. It is the leading cause of mortality in neonatal and weaned piglets in pig husbandry, causing diarrhea and significant harm to the industry. Furthermore, the frequent and intensive use of antimicrobials for the prevention of diseases, particularly gastrointestinal diseases, may promote the selection of multidrug-resistant (MDR) strains. These resistant genotypes can be transmitted through the excrement of animals, including swine. It is common practice to use porcine manure processed by biodigesters as fertilizer. This study aimed to examine the antimicrobial susceptibility, the presence of virulence genes frequently associated with pathotypes of intestinal pathogenic E. coli (InPEC), and antimicrobial resistance genes (ARGs) of 28 E. coli isolates collected from swine manure fertilizers. In addition, the enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) technique was used to investigate the genetic relationship among the strains. Using disk diffusion, the antimicrobial susceptibility profiles of the strains were determined. Using polymerase chain reaction (PCR), 14 distinct virulence genes associated with the most prevalent diarrhea and intestinal pathogenic E. coli (DEC/InPEC) and five ARGs were analyzed. All isolates tested positive for multidrug resistance. There was no detection of any of the 14 virulence genes associated with InPECs, indicating the presence of an avirulent commensal microbiota. Molecular classification by ERIC-PCR revealed that the majority of isolates (27 isolates) coalesced into a larger cluster with a genetic similarity of 47.7%; only one strain did not cluster in this cluster, indicating a high level of genetic diversity among the analyzed isolates. Thus, it is of the utmost importance to conduct epidemiological surveillance of animal breeding facilities in order to determine their microbiota and formulate plans to reduce the use of antimicrobials and improve animal welfare.

State of microalgae-based swine manure digestate treatment: An overview.

Global pork production has an annual growth of approximately 2.1%, and its economic and environmental impact are related with the treatment of waste in the production chain. There is little evidence of research advances to generate alternatives for using these wastes. The lack of research related to microalgae cultivation using digestate produced by porcine residues generates negative environmental impact, inadequate and inefficient technologies, low recovery and use of waste and loss of value and competitiveness in the market. The available literature focuses mainly on the treatment of anaerobic digestion liquid effluents for the removal of components, but not on the generation of value-added products. Therefore, there is a need to collect the available information, analyze it and propose other new methodologies. This article presents the information obtained from conducting a systematic review of the literature with a bibliometric and a comparative analysis; achieving an analysis of the temporal and geographical distribution, the main topics, the most influential players, the degree of maturity of the research and different strategies collected for microalgae-based swine manure digestate treatment. In this way, it was possible to capture an overview of the current state of the development of research focused on the use of digestate for the cultivation of microalgae, visualizing important aspects as the evolution of publications, identifying China and USA as the main players in research, biomass and wastewater as potential topics also Spirulina, Astaxanthin and beta-carotene as the main products based on microalgae. Thus, achieving an structure, organized and synthesized landscape of scientific and technological knowledge available for the proposal of investigations that allow the use of anaerobic digestion liquid effluents as cultivation medium for microalgae.

Different Swine Production Systems Can Shape Slurry Resistome at Mechanism and Class Levels Based on Swine Manure Evaluation.

Antimicrobial resistance is a major threat to public health. Antimicrobial use in animal husbandry is a major concern since it can favor an increase in antimicrobial resistance among farms. Herein, we aim to better understand and characterize the main resistome profiles in microbial communities found in pig farms. Sampling of swine manure was performed in two different timepoints (October 2019 and January 2020) in each of the 14 different swine farms, located in the mesoregion of Western Santa Catarina state in Brazil, a pole of swine product production of worldwide importance. Samples were divided into three groups: farms with the opened regimen and no usage of antimicrobials (F1; n = 10), farms with the closed regimen and usage of antimicrobials (F2; n = 16), and farms with the closed regimen and no usage of antimicrobials (F3; n = 2). The metagenomic evaluation was performed to obtain and identify genetic elements related to antimicrobial resistance using nanopore sequencing. We used ResistoXplorer software to perform composition, alpha and beta diversity, and clustering analysis. In addition, PCR reactions were performed to confirm the presence or absence of seven different beta-lactamase family genes and five phosphoethanolamine transferase gene variants clinically relevant. Our findings based on the identification of resistance genes at the mechanism level showed a prevalence of alteration of the drug target (72.3%) profile, followed by drug inactivation (17.5%) and drug efflux (10.1%). We identified predominantly aminoglycosides (45.3%), tetracyclines (15.9%), and multiclass (11,2%) resistance genes. PCoA analysis indicates differences between F1 and F2 profiles. F2 samples showed increased diversity when compared to the F1 group. In addition, herein we first report the identification of mcr-4 in a slurry sample (C1F1.1) in Santa Catarina State. In general, our findings reinforce that many factors on the practices of animal husbandry are involved in the resistome profile at the mechanism and class levels. Further studies to better understand microbiome and mobilome aspects of these elements are necessary to elucidate transmission pathways between different bacteria and environments.

Aplicabilidade de sistema reator anaeróbio compartimentado seguida de filtro anaeróbio no tratamento de efluentes de suinocultura de pequeno porte / Applicability of compartmentalized anaerobic reactor system followed by anaerobic filter on small swine wastewater treatment

RESUMO O objetivo deste trabalho foi avaliar o desempenho de um sistema composto de reator anaeróbio compartimentado (RAC) seguido por filtro anaeróbio (FA), tratando águas residuárias de uma granja suinícola de pequeno porte. Foram analisados os parâmetros: pH, ácidos voláteis totais (AVT), demanda química de oxigênio total (DQOt), demanda química de oxigênio dissolvida (DQOdiss), demanda química de oxigênio devida aos sólidos suspensos (DQOSS), sólidos suspensos totais (SST), sólidos suspensos voláteis (SSV) e nitrogênio total kjeldhal (NTK). Os valores efluentes no RAC e no FA, de DQOt, SST e NTK, foram 6.633 e 4.361 mg.L-1, 2.418 e 595 mg.L-1 e 888 e 954 mg.L-1, respectivamente, para uma carga orgânica volumétrica (COV) de 7,01 kgDQO(m3.d)-1 no RAC e 28,3 kgDQO(m3.d)-1 no FA, e tempo de detenção hidráulico de 70,5 e 17,8 h no RAC e no FA, respectivamente. O sistema RAC seguido de FA mostrou eficiência de remoção média de 88,8% para DQOt e 80,5% de SST. Conclui-se que o sistema de tratamento anaeróbio, composto por RAC seguido de FA, foi eficiente na remoção de DQO e SST, possibilitando, nas condições operacionais impostas, alcançar valores médios de remoção acima de 80%, tornando-se uma ótima alternativa de tratamento de efluentes de suinocultura para granjas de pequeno porte.

ABSTRACT The aim of this work was to assess the performance of a system composed by a compartmentalized anaerobic reactor (CAR) followed by anaerobic filter (AF) in the treatment of the wastewater of as mall pigfarm.The following parameters were analyzed: pH, total volatilefatty acids (VFA), total demand of chemical oxygen (DCOt), dissolved chemical oxygen demand (CODdiss), chemical oxygen demand due to suspended solids (CODSS), total suspended solids (TSS), volatile suspended solids (VSS) and total kjeldhal nitrogen (TKN). The effluents and AF values for the CAR, the DCOt, TSS and TKN were 6,633 and 4,361mg.L-1, 2,418 and 595 mg.L-1, and 888 and 954 mg.L-1, respectively, for a volumetric organic load (VOL) of 7.01 kgDCO(m3.d)-1 in CAR and 28.3 kgDCO(m3.d)-1 in AF, and hydraulic detention time of 70.5 hand 17.8 hin CAR and AF, respectively. The CAR system followed by FA presented a mean removal efficiency of 88.8% for COD and 80.5% for TSS. It is concluded that the anaerobic treatment system consisting of CAR followed by AF was efficient in removing DCO and TSS, allowing with the imposed operating conditions to achieve average removal values above 80%, becoming a great alternative for wastewater treatment in small swinefarms.

Fertilization with liquid swine manure increases productivity and improves the quality of Urochloa decumbens / Fertilização com dejeto líquido suíno aumenta a produtividade e melhora a qualidade da Urochloa decumbens

This study aims at assessing the influence of mineral fertilization and biofertilizer from liquid swine manure (LSM) in productivity, levels of crude protein (CP), neutral detergent fiber (NDF) and Acid Detergent Fiber (ADF) of Urochloa decumbens. The experiment was carried out in a red Latosol under a Urochloa decumbens pasture, in a state of degradation with loss of vigor and forage productivity. The delineation chosen for this study was randomized blocks with six treatments (control without fertilization, control with use of mineral fertilizer and four unique concentrations of liquid swine manure biofertilizer (61 m3 ha-1, 121 m3 ha-1, 181 m3 ha-1, 241 m3 ha-1)). The application of residue has been split in two times on the surface of the soil, by means of a distributor of liquid waste with a fan-shaped application. Yield and bromatological features were evaluated for two years. Fertilization with LSM can fully replace mineral fertilizing in Urochloa decumbens pasture systems, resulting in an increase, as a result, in the dry mass yield from the dosage of 121 m3 ha-1 and the reduction in fiber content in neutral and acid detergent. The increments in yield were observed in the second year, while improvement in bromatological quality was observed from the first application of LSM.

Objetivou-se avaliar a influência da fertilização mineral e do biofertilizante de dejeto líquido suíno (DLS) na produtividade, teores de Proteína Bruta (PB), Fibra em Detergente Neutro (FDN) e Fibra em Detergente Ácido (FDA) da Urochloa decumbens. O experimento foi desenvolvido em um Latossolo vermelho sob pastagem de Urochloa decumbens, em estado de degradação evoluída com perda de vigor e produtividade da forrageira. O delineamento utilizado foi em blocos casualizados com seis tratamentos (controle sem fertilização, controle com uso de fertilizante mineral e quatro concentrações exclusivas de biofertilizante de dejeto líquido suíno (61 m3 ha-1, 121 m3 ha-1, 181 m3 ha-1, 241 m3 ha-1)). A aplicação do resíduo foi parcelada em duas vezes na superfície do solo, por meio de um distribuidor de resíduos líquidos com a aplicação em leque. Foram avaliadas por dois anos a produtividade, e as caracterizações bromatológica foliares. A fertilização com DLS pode substituir integralmente a fertilização mineral no sistema de pastejo de Urochloa decumbens, aumentando a produtividade de massa seca a partir da dosagem de 121 m3 ha-1 e redução no teor de Fibras em detergente neutro e ácido. Os incrementos na produtividade foram observados no segundo ano, ao passo que melhoria na qualidade gramatológicas foi observada a partir da primeira aplicação do DLS.

Wastewater from swine farming in the growth and nutrition of Khaya senegalensis (DESR. ) A Juss seedlings / Água residuária da suinocultura no crescimento e nutrição de mudas de khaya senegalensis (DESR. ) a juss

This study evaluated the use of wastewater from swine farming in the growth and nutritional balance of Khaya senegalensis (Desr.) A. Juss. (African mahogany) seedlings. The experiment was setup in a shade house on the Professor Cinobelina Elvas Campus of the Federal University of Piauí, in Bom Jesus, in the State of Piauí, Brazil. The experimental design was completely randomised, with five concentrations of swine farm wastewater (SFW) (0, 25, 50, 75 and 100%) added to the irrigation water. The growth and nutritional balance of the seedlings were evaluated 100 days after sowing, by measuring shoot height (H), stem diameter (SD), number of leaves (NL), total chlorophyll (TC), leaf area (LA), shoot dry weight (SDW) and root dry weight (RDW), and by calculating the total dry weight (TDW), leaf (LBA), stem (SBA), and root (RBA) biomass allocation, Dickson Quality Index (DQI) and average Nutritional Balance Index (NBIm). It was found that K. senegalensis seedlings responded to the SFW, showing the best results for growth and nutritional balance at concentrations of around 50%.

Este trabalho foi realizado com o objetivo de avaliar a utilização da água residuária da suinocultura no crescimento e no balanço nutricional de mudas de Khaya senegalensis (Desr.) A. Juss. (mogno-africano). O experimento foi instalado no Campus Profa. Cinobelina Elvas, Universidade Federal do Piauí, em Bom Jesus, PI, dentro de uma casa de sombra. O experimento foi implantado em delineamento inteiramentecasualizado, com cinco concentrações de água residuária de suinocultura (ARS) (0; 25; 50; 75 e 100%) na água de irrigação. O crescimento e balanço nutricional das mudas foram avaliados aos 100 dias após a semeadura, com a mensuração da altura da parte aérea (H), diâmetro do coleto (DC), número de folhas (NF), clorofila total (CT), área foliar (AF), massa seca da parte aérea (MSPA) e do sistema radicular (MSR) e calculadas a massa seca total (MST), as alocações de biomassa foliar (ABF), caule (ABC), raízes (ABR), o Índice de Qualidade de Dickson (IQD) e Índice de Balanço Nutricional médio (IBNm). Constatou-se que a as mudas de K. senegalensis responderam à ARS, apresentando os melhores resultados de crescimento e equilíbrio nutricional em concentrações em torno de 50%.

Efeito da variação da carga orgânica volumétrica natural na produção de biogás a partir de dejeto suíno em diferentes tempos de retenção hidráulica / The effect of organic loading rate's natural variation on the biogas yields from swine manure digestion at different hydraulic retention time

RESUMO O estudo sobre reatores anaeróbios em laboratório prioriza o controle de determinados parâmetros que são de difícil controle em campo, o que dificulta o processo de scale-up. Entre os parâmetros está a carga orgânica volumétrica (COV). No caso do dejeto suíno, esse parâmetro pode sofrer alterações diárias em função das condições climáticas, das mudanças no manejo, da alimentação dos animais, entre outros. A fim de verificar o impacto dessa variação na produtividade de biogás, foi analisado o comportamento de um reator anaeróbio de mistura completa (CSTR) em dois diferentes tempos de retenção hidráulica (TRHs) - 20 e 30 dias - durante um período total de 180 dias. Os resultados demonstraram que a elevação da COV resulta em piora da eficiência de tratamento, bem como na produção de biogás. O ensaio com TRH de 20 dias obteve uma melhor resposta às alterações de carga orgânica.

ABSTRACT The study of anaerobic digesters at laboratory focuses on the control of certain parameters that are not so easy to control in the field, which makes it difficult to process scale-up. One example is the Organic Loading Rate. To swine manure, this parameter can change daily due to weather conditions, management practices, feeding and others. In order to verify the impact of this fluctuation on biogas yield, the behavior of a CSTR reactor was analyzed in two different Hydraulic Retention Times (20 and 30 days) for a total period of 180 days. The result demonstrated that the increase of OLR result in a decrease of treatment efficiency as well as biogas yields. The study with HRT of 20 days had a better response to changes in organic load.

Enterobacteria associated with houseflies (Musca domestica) as an infection risk indicator in swine production farms.

Houseflies (Musca domestica) spend part of their life development on animal or human manure. Manure is high in pathogenic microbes; thus, houseflies have been known as a mechanical vector for various important zoonotic diseases. Therefore, the present study showcases captured houseflies from intensive swine production regions (which are areas of high manure concentration) in Southern Brazil, and analyses their bodies' to the presence of Escherichia coli and Salmonella sp. and the sensitivity of these bacteria to various antibiotics. Additionally, Quantitative Microbiology Risk Assessment was performed simulating the contamination of lettuce by flies' bacteria and subsequent lettuce consumption by an adult human being. Houseflies were captured in swine buildings and farm houses from five farms. E. coli quantification values ranged from 104 to 106 CFU/20 flies, and all sampling sites had positive results from bacteria presence in the collected houseflies. On the other hand, Salmonella sp. presence was observed in only three farms, where the quantification ranged from 102 to 105 CFU/20 flies. The bacteria showed to be resistant to at least two from the four tested antibiotics (ampicillin, Cefalotin, Ciprofloxacin and Norfloxacin) antibiotics used in human or veterinary medicine. Infection probability analyses showed risk of human infection by E.coli, indicating possible transmission of zoonotic pathogens through flies. In this context, it was possible to conclude that there is a need for flies control, especially in swine farms where zoonotic pathogens can be abundant, to minimize the health impact of the vectorization of enteric bacteria.

Propidium Monoazide Coupled with PCR Predicts Infectivity of Enteric Viruses in Swine Manure and Biofertilized Soil.

The use of propidium monoazide (PMA) coupled with real-time PCR (RT-qPCR or qPCR for RNA or DNA viruses, respectively) was assessed to discriminate infectious enteric viruses in swine raw manure, swine effluent from anaerobic biodigester (AB) and biofertilized soils. Those samples were spiked either with infectious and heat-inactivated human adenovirus-2 (HAdV-2) or mengovirus (vMC0), and PMA-qPCR/RT-qPCR allowed discriminating inactivated viruses from the infective particles, with significant reductions (>99.9%). Then, the procedure was further assayed to evaluate the presence and stability of two non-cultivable viruses (porcine adenovirus and rotavirus A) in natural samples (swine raw manure, swine effluent from AB and biofertilized soils); it demonstrated viral inactivation during the storage period at 23 °C. As a result, the combination of PMA coupled to real-time PCR can be a promising alternative for prediction of viral infectivity in comparison to more labour-intensive and costly techniques such as animal or tissue-culture infectivity methods, and for those viruses that do not have currently available cell culture techniques.

Influence of solid-liquid separation strategy on biogas yield from a stratified swine production system.

As the fourth largest swine producer and exporter, Brazil has increased its participation in the global swine production market. Generally, these units concentrate a large number of animals and generate effluents that must be correctly managed to prevent environmental impacts, being anaerobic digestion is an interesting alternative for treating these effluents. The low-volatile solid concentration in the manure suggests the need for solid-liquid separation as a tool to improve the biogas generation capacity. This study aimed to determine the influence of simplified and inexpensive solid-liquid separation strategies (screening and settling) and the different manures produced during each swine production phase (gestating and farrowing sow houses, nursery houses and finishing houses) on biogas and methane yield. We collected samples in two gestating sow houses (GSH-a and GSH-b), two farrowing sow houses (FSH-a and FSH-b), a nursery house (NH) and a finishing house (FH). Biochemical methane potential (BMP) tests were performed according to international standard procedures. The settled sludge fraction comprised 20-30% of the raw manure volume, which comprises 40-60% of the total methane yield. The methane potential of the settled sludge fraction was approximately two times higher than the methane potential of the supernatant fraction. The biogas yield differed among the raw manures from different swine production phases (GSH-a 326.4 and GSH-b 577.1; FSH-a 860.1 and FSH-b 479.2; NH -970.2; FH 474.5 NmLbiogas.gVS(-1)). The differences were relative to the production phase (feed type and feeding techniques) and the management of the effluent inside the facilities (water management). Brazilian swine production has increased his participation in the global market, been the fourth producer and the fourth exporter. The segregation of swine production in multiple sites has increased its importance, due to the possibilities to have more specialized units. Generally, these units concentrate a large number of animals and generate effluents that must be correctly managed to avoid environmental impact. Due to the biodegradability of manure, anaerobic digestion is an interesting alternative to treat these effluents. The low volatile solid concentration in the swine manure suggests the need for solid-liquid separation as a tool to improve biogas generation capacity. The present study aimed to determine the influence of simplified and cheap solid-liquid separation strategies (based on screening and settling) and different manure of each swine production phases (gestating and farrowing sows houses, nursery houses and finishing houses) on biogas and methane yield. We collected samples in two gestating sows house (GSH-a and GSH-b), two farrowing sows house (FSH-a and FSH-b), a nursery house (NH) and a finishing house (FH). The Biochemical Methane Production (BMP) tests were performed according to international standard procedure (VDI 4630). The settled sludge fraction responds for 20-30% of raw manure volume, producing 40-60% of the total methane yield. The methane potential of settled sludge fraction was about 2 times higher than the supernatant fraction. There are differences on biogas yield between the raw manure of different swine production phases (GSH-a 326.4 and GSH-b 577.1; FSH-a 860.1 and FSH-b 479.2; NH 970.2; FH 474.5 NmLbiogas.gVS(-1)). The differences are relative to production phase (feed type, feeding techniques, etc.), but also the management of the effluent inside the facilities (water management).

Human and animal enteric virus in groundwater from deep wells, and recreational and network water.

This study was designed to assess the presence of human adenovirus (HAdV), rotavirus-A (RVA), hepatitis A virus (HAV), and porcine circovirus-2 (PCV2) in groundwater from deep wells, and recreational and network waters. The water samples were collected and concentrated and the virus genomes were assessed and quantified by quantitative PCR (qPCR). Infectious HAdV was evaluated in groundwater and network water samples by integrated cell culture using transcribed messenger RNA (mRNA) (ICC-RT-qPCR). In recreational water samples, HAdV was detected in 100 % (6/6), HAV in 66.6 % (4/6), and RVA in 66.6 % (4/6). In network water, HAdV was detected in 100 % (6/6) of the samples (these 83 % contained infectious HAdV), although HAV and RVA were not detected and PCV2 was not evaluated. In groundwater from deep wells, during rainy period, HAdV and RVA were detected in 80 % (4/5) of the samples, and HAV and PCV2 were not detected; however, during dry period, HAdV and RVA were detected in 60 % (3/5), HAV in only one sample, and PCV2 in 60 % (4/5). In groundwater, all samples contained infectious HAdV. PCV2 presence in groundwater is indicative of contamination caused by swine manure in Concórdia, Santa Catarina, Brazil. The disinfection of human and animal wastes is urgent, since they can contaminate surface and groundwater, being a potential threat for public and animal health.

Soil microbial properties after long-term swine slurry application to conventional and no-tillage systems in Brazil.

Swine waste can be used as an agricultural fertilizer, but large amounts may accumulate excess nutrients in soil or contaminate the surrounding environment. This study evaluated long-term soil amendment (15 years) with different levels of swine slurry to conventional (plow) tillage (CT) and no tillage (NT) soils. Long-term swine slurry application did not affect soil organic carbon. Some chemical properties, such as calcium, base saturation, and aluminum saturation were significantly different within and between tillages for various application rates. Available P and microbial parameters were significantly affected by slurry addition. Depending on tillage, soil microbial biomass and enzyme activity increased up to 120 m(3) ha(-1) year(-1) in all application rates. The NT system had higher microbial biomass and activity than CT at all application levels. There was an inverse relationship between the metabolic quotient (qCO2) and MBC, and the qCO2 was 53% lower in NT than CT. Swine slurry increased overall acid phosphatase activity, but the phosphatase produced per unit of microbial biomass decreased. A comparison of data obtained in the 3rd and 15th years of swine slurry application indicated that despite slurry application the CT system degraded with time while the NT system had improved values of soil quality indicators. For these Brazilian oxisols, swine slurry amendment was insufficient to maintain soil quality parameters in annual crop production without additional changes in tillage management.

Utility of specific biomarkers to assess safety of swine manure for biofertilizing purposes.

Swine production is an important economic activity in Brazil, and there is interest in the development of clean production mechanisms to support sustainable agro-industrial activities. The biomass derived from swine manure has good potential to be used as a biofertilizer due to its high nutrient concentration. However, the land application of manure should be based on safety parameters such as the presence of pathogens that can potentially infect animals and people. This study was designed to assess the presence of porcine circovirus-2 (PCV2), porcine adenovirus (PAdV), rotavirus-A (RV-A) and Salmonella spp. in liquid manure, as well the infectivity of two genotypes of circovirus-2 (PCV2a and PCV2b) present in liquid manure. Three swine farms were evaluated: 1) a nursery production farm (manure analyzed before and after anaerobic biodigestion), 2) a grow-finish production farm (analyzed before and after anaerobic biodigestion), and 3) a second grow-finish production farm (raw manure-affluent). PCV2, PAdV and RV-A were present before and after anaerobic biodigestion (either affluent or effluent) at all farms. Salmonella spp. were detected at farm 1 (affluent and effluent) and farm 3 (raw manure-affluent) but not farm 2 (affluent and effluent). When the ability of the anaerobic biodigestion process to reduce viral concentration was evaluated, no significant reduction was observed (P>0.05). Both the PCV2a and PCV2b genotypes were detected, suggesting viral co-infection in swine production. The results revealed infectious PCV2 even after anaerobic biodigestion treatment. The presence of Salmonella spp. and enteric viruses, especially infectious PCV2, in the final effluent from the anaerobic biodigester system suggests that the process is inefficient for pathogen inactivation. Due to the prevalence and infectivity of PCV2 and considering the successful use of molecular methods coupled to cell culture for detecting infectious PCV2, we suggest that this virus can be used as a bioindicator in swine manure treatment systems to check the efficiency of pathogen inactivation and ensure the production of safe biofertilizers from swine manure.

Perda de nutrientes via escoamento superficial no sistema plantio direto sob adubação mineral e orgânica / Nutrient losses by surface runoff in no-till system soil under mineral and organic fertilization

O sistema de plantio direto (SPD) tem contribuído para melhorias nos solos agrícolas, dentre elas o aumento da concentração de nutrientes no solo. No entanto, tem se verificado que este aumento ocorre principalmente na camada superficial do solo, o que contribui para que ocorram perdas de nutrientes através de enxurradas, com danos econômicos e ambientais. Este trabalho avaliou a concentração total dos nutrientes potássio (K), cálcio (Ca), magnésio (Mg) e cobre (Cu) e do K nas formas solúvel e particulado, na enxurrada ocasionada por chuvas simuladas com diferentes intensidades aplicadas sobre solo manejado por cinco anos sob SPD, após aplicação superficial de adubo orgânico ou mineral. Os adubos não afetaram a concentração dos nutrientes estudados, exceto na condição da chuva mais intensa e solo sob adubação orgânica, onde a enxurrada apresentou maior concentração de K solúvel e K total. Independente do tipo de adubo utilizado, a chuva de maior intensidade ocasionou maior concentração de todos os nutrientes e formas estudados, evidenciando os benefícios econômicos e ambientais da adoção de práticas de contenção da enxurrada, mesmo em áreas cultivadas sob SPD.

The no-tillage system, has contributed to improvements in agricultural soils, among them the like increases in nutrient concentrations.of mineral elements in soil. However, it has been found that this increase concentration occurs mainly in the first few centimeters of the superficial top layer and so risks of nutrient losses by runoff, and associated of the soil, which concurs for losses of mineral elements through of runoff, with economic and environmental damages, are high. This study evaluated the total concentration, in runoff, of mineral elements potassium (K), calcium (Ca), magnesium (Mg) and copper (Cu) in total form and the soluble and particulate concentration form of K, in runoff caused to simulated rainfall of different intensitiesy applied over soil under no-till system and submitted to simulated after application of organic or mineral fertilizers in no-till soil. The fertilizers did not affect the concentration of mineral elements studied nutrients in runoff, except in condition of more high intense rainfall and soil under after organic manure application, which promoted runoff with showed the higher highest concentration of soluble and total K. Independent Irrespective of the type of fertilizers utilized type, the rainfall of higher intensity resulted in higher concentrations of all the mineral elements and forms studied, either in total, soluble or particulate form, evidencing the economic and environmental benefices, risks and suggesting that with the adoption of practices for runoff contention of runoff practices must be adopted , even in tilled croplands areas managed under no-till system.

Bacterial biodiversity from an anaerobic up flow bioreactor with ANAMMOX activity inoculated with swine sludge

The present study aimed to describe the bacterial community present at an anaerobic up flow bioreactor with ANAMMOX activity, inoculated with the sludge from the anaerobic pond of a swine slurry treatment system. The description was based on the molecular DNA techniques using primers for amplification of complete 16S rRNA gene and also new primers to amplify smaller fragments from 16S rRNA. During the bioreactor operation time, the bacterial community changed significantly, increasing the nitrogen removal efficiency, reaching after 500 days a removal rate of 94 percent. The complete PCR amplification of 16S rRNA gene generated 17 clones, where three presented similarity with Candidatus Jettenia asiatica (97 percent), twelve with Janthinobacterium (99 percent) and two with uncultured clones. The PCR amplification of 436 base pairs had generated 12 clones, of which eight presented 96-100 percent similarity with Candidatus Anammoxoglobus propionicus, Planctomycete KSU-1 and one with Pseudomonas sp. (99 percent) and three with uncultured clones.

Chemical attributes of a South Brazilian Oxisol fertilized with swine manure and soluble fertilizer / Atributos químicos em Latossolo Vermelho fertilizado com dejeto suíno e adubo solúvel

The use of swine manure as fertilizer is the main method for final disposal of this waste. However, the dose applied must not exceed the reception capacity of the soil, to avoid the excess of elements that have potential pollutants, particularly P, Cu and Zn. In order to evaluate changes in chemical attributes of a South Brazilian Oxisol, caused by six annual applications of swine manure at the rates 0 (control), 25, 50, 100 and 200 m3 ha-1, soluble fertilizer, and manure supplemented with soluble fertilizer, a field experiment was conducted in Campos Novos, SC, in the period from 2001 to 2007. Each year, successive crops of corn (Zea mays) and oat (Avena strigosa) were grown under no-tillage. In August 2007, the soil was sampled at depths of 0-2, 4-6, 9-11, 19-21, 29-31 and 39-41 cm. Compared to the control treatment, the manure did not affect the pH and concentration of exchangeable Al and Mg, but it increased the exchangeable Ca, although only at the rate of 200 m3 ha-1, at 0-2 and 4-6 cm depths. At the first depth, soluble fertilizer decreased the pH in relation to other treatments, and at 4-6 cm depth the soluble fertilizer decreased this attribute only in relation to manure applied at the highest dose. The manure in doses of 100 and 200 m3 ha-1 increased significantly the content of extractable P at depths 0-2 and 4-6 cm, including in relation to soluble fertilizer. At th

O uso do dejeto suíno como fertilizante é a alternativa mais utilizada para o descarte desse resíduo. Entretanto, a dose aplicada não deve ultrapassar a capacidade de adsorção do solo a fim de evitar que o excesso de elementos, especialmente P, Cu e Zn, possa atuar como poluente. O presente estudo foi conduzido com o objetivo de avaliar as variações nos atributos químicos de um Latossolo Vermelho distroférrico provocadas por seis aplicações anuais de dejeto suíno (DJ), nas doses 0 (testemunha), 25, 50, 100 e 200 m3 ha-1, de adubo solúvel (AS), e de dejeto complementado com adubo solúvel (DJ+AS). O experimento foi conduzido a campo, no município de Campos Novos, SC, no período de 2001 a 2007. Anualmente, foram cultivados milho (Zea mays) e aveia (Avena strigosa) em cultivos sucessivos, no sistema plantio direto. Em agosto de 2007, o solo foi amostrado nas profundidades de 0-2, 4-6, 9-11, 19-21, 29-31 e 39-41 cm. O DJ não afetou o pH e os teores de Al e Mg trocáveis do solo, mas aumentou o teor de Ca trocável, embora somente na dose 200 m3 ha-1 e na profundidade 0-2 cm. Nesta, o AS diminuiu o pH em relação aos demais tratamentos, tendo também diminuído este atributo na profundidade 4-6 cm, porém, somente em relação ao DJ na maior dose aplicada. O DJ, em doses de 100 e 200 m3 ha-1, aumentou o teor de P extraível nas camadas de 0-2 e 4-6 cm, inclusive em relação ao AS. Na maior dos

Perda de nutrientes via escoamento superficial no sistema plantio direto sob adubação mineral e orgânica

The no-tillage system, has contributed to improvements in agricultural soils, among them the like increases in nutrient concentrations.of mineral elements in soil. However, it has been found that this increase concentration occurs mainly in the first few centimeters of the superficial top layer and so risks of nutrient losses by runoff, and associated of the soil, which concurs for losses of mineral elements through of runoff, with economic and environmental damages, are high. This study evaluated the total concentration, in runoff, of mineral elements potassium (K), calcium (Ca), magnesium (Mg) and copper (Cu) in total form and the soluble and particulate concentration form of K, in runoff caused to simulated rainfall of different intensitiesy applied over soil under no-till system and submitted to simulated after application of organic or mineral fertilizers in no-till soil. The fertilizers did not affect the concentration of mineral elements studied nutrients in runoff, except in condition of more high intense rainfall and soil under after organic manure application, which promoted runoff with showed the higher highest concentration of soluble and total K. Independent Irrespective of the type of fertilizers utilized type, the rainfall of higher intensity resulted in higher concentrations of all the mineral elements and forms studied, either in total, soluble or particulate form, evidencing the economic and environmental benefices, risks and suggesting that with the adoption of practices for runoff contention of runoff practices must be adopted , even in tilled croplands areas managed under no-till system.

O sistema de plantio direto (SPD) tem contribuído para melhorias nos solos agrícolas, dentre elas o aumento da concentração de nutrientes no solo. No entanto, tem se verificado que este aumento ocorre principalmente na camada superficial do solo, o que contribui para que ocorram perdas de nutrientes através de enxurradas, com danos econômicos e ambientais. Este trabalho avaliou a concentração total dos nutrientes potássio (K), cálcio (Ca), magnésio (Mg) e cobre (Cu) e do K nas formas solúvel e particulado, na enxurrada ocasionada por chuvas simuladas com diferentes intensidades aplicadas sobre solo manejado por cinco anos sob SPD, após aplicação superficial de adubo orgânico ou mineral. Os adubos não afetaram a concentração dos nutrientes estudados, exceto na condição da chuva mais intensa e solo sob adubação orgânica, onde a enxurrada apresentou maior concentração de K solúvel e K total. Independente do tipo de adubo utilizado, a chuva de maior intensidade ocasionou maior concentração de todos os nutrientes e formas estudados, evidenciando os benefícios econômicos e ambientais da adoção de práticas de contenção da enxurrada, mesmo em áreas cultivadas sob SPD.

Perda de nutrientes via escoamento superficial no sistema plantio direto sob adubação mineral e orgânica

The no-tillage system, has contributed to improvements in agricultural soils, among them the like increases in nutrient concentrations.of mineral elements in soil. However, it has been found that this increase concentration occurs mainly in the first few centimeters of the superficial top layer and so risks of nutrient losses by runoff, and associated of the soil, which concurs for losses of mineral elements through of runoff, with economic and environmental damages, are high. This study evaluated the total concentration, in runoff, of mineral elements potassium (K), calcium (Ca), magnesium (Mg) and copper (Cu) in total form and the soluble and particulate concentration form of K, in runoff caused to simulated rainfall of different intensitiesy applied over soil under no-till system and submitted to simulated after application of organic or mineral fertilizers in no-till soil. The fertilizers did not affect the concentration of mineral elements studied nutrients in runoff, except in condition of more high intense rainfall and soil under after organic manure application, which promoted runoff with showed the higher highest concentration of soluble and total K. Independent Irrespective of the type of fertilizers utilized type, the rainfall of higher intensity resulted in higher concentrations of all the mineral elements and forms studied, either in total, soluble or particulate form, evidencing the economic and environmental benefices, risks and suggesting that with the adoption of practices for runoff contention of runoff practices must be adopted , even in tilled croplands areas managed under no-till system.

O sistema de plantio direto (SPD) tem contribuído para melhorias nos solos agrícolas, dentre elas o aumento da concentração de nutrientes no solo. No entanto, tem se verificado que este aumento ocorre principalmente na camada superficial do solo, o que contribui para que ocorram perdas de nutrientes através de enxurradas, com danos econômicos e ambientais. Este trabalho avaliou a concentração total dos nutrientes potássio (K), cálcio (Ca), magnésio (Mg) e cobre (Cu) e do K nas formas solúvel e particulado, na enxurrada ocasionada por chuvas simuladas com diferentes intensidades aplicadas sobre solo manejado por cinco anos sob SPD, após aplicação superficial de adubo orgânico ou mineral. Os adubos não afetaram a concentração dos nutrientes estudados, exceto na condição da chuva mais intensa e solo sob adubação orgânica, onde a enxurrada apresentou maior concentração de K solúvel e K total. Independente do tipo de adubo utilizado, a chuva de maior intensidade ocasionou maior concentração de todos os nutrientes e formas estudados, evidenciando os benefícios econômicos e ambientais da adoção de práticas de contenção da enxurrada, mesmo em áreas cultivadas sob SPD.

Perda de nutrientes via escoamento superficial no sistema plantio direto sob adubação mineral e orgânica

The no-tillage system, has contributed to improvements in agricultural soils, among them the like increases in nutrient concentrations.of mineral elements in soil. However, it has been found that this increase concentration occurs mainly in the first few centimeters of the superficial top layer and so risks of nutrient losses by runoff, and associated of the soil, which concurs for losses of mineral elements through of runoff, with economic and environmental damages, are high. This study evaluated the total concentration, in runoff, of mineral elements potassium (K), calcium (Ca), magnesium (Mg) and copper (Cu) in total form and the soluble and particulate concentration form of K, in runoff caused to simulated rainfall of different intensitiesy applied over soil under no-till system and submitted to simulated after application of organic or mineral fertilizers in no-till soil. The fertilizers did not affect the concentration of mineral elements studied nutrients in runoff, except in condition of more high intense rainfall and soil under after organic manure application, which promoted runoff with showed the higher highest concentration of soluble and total K. Independent Irrespective of the type of fertilizers utilized type, the rainfall of higher intensity resulted in higher concentrations of all the mineral elements and forms studied, either in total, soluble or particulate form, evidencing the economic and environmental benefices, risks and suggesting that with the adoption of practices for runoff contention of runoff practices must be adopted , even in tilled croplands areas managed under no-till system.

O sistema de plantio direto (SPD) tem contribuído para melhorias nos solos agrícolas, dentre elas o aumento da concentração de nutrientes no solo. No entanto, tem se verificado que este aumento ocorre principalmente na camada superficial do solo, o que contribui para que ocorram perdas de nutrientes através de enxurradas, com danos econômicos e ambientais. Este trabalho avaliou a concentração total dos nutrientes potássio (K), cálcio (Ca), magnésio (Mg) e cobre (Cu) e do K nas formas solúvel e particulado, na enxurrada ocasionada por chuvas simuladas com diferentes intensidades aplicadas sobre solo manejado por cinco anos sob SPD, após aplicação superficial de adubo orgânico ou mineral. Os adubos não afetaram a concentração dos nutrientes estudados, exceto na condição da chuva mais intensa e solo sob adubação orgânica, onde a enxurrada apresentou maior concentração de K solúvel e K total. Independente do tipo de adubo utilizado, a chuva de maior intensidade ocasionou maior concentração de todos os nutrientes e formas estudados, evidenciando os benefícios econômicos e ambientais da adoção de práticas de contenção da enxurrada, mesmo em áreas cultivadas sob SPD.

Chemical attributes of a South Brazilian Oxisol fertilized with swine manure and soluble fertilizer / Atributos químicos em Latossolo Vermelho fertilizado com dejeto suíno e adubo solúvel

The use of swine manure as fertilizer is the main method for final disposal of this waste. However, the dose applied must not exceed the reception capacity of the soil, to avoid the excess of elements that have potential pollutants, particularly P, Cu and Zn. In order to evaluate changes in chemical attributes of a South Brazilian Oxisol, caused by six annual applications of swine manure at the rates 0 (control), 25, 50, 100 and 200 m3 ha-1, soluble fertilizer, and manure supplemented with soluble fertilizer, a field experiment was conducted in Campos Novos, SC, in the period from 2001 to 2007. Each year, successive crops of corn (Zea mays) and oat (Avena strigosa) were grown under no-tillage. In August 2007, the soil was sampled at depths of 0-2, 4-6, 9-11, 19-21, 29-31 and 39-41 cm. Compared to the control treatment, the manure did not affect the pH and concentration of exchangeable Al and Mg, but it increased the exchangeable Ca, although only at the rate of 200 m3 ha-1, at 0-2 and 4-6 cm depths. At the first depth, soluble fertilizer decreased the pH in relation to other treatments, and at 4-6 cm depth the soluble fertilizer decreased this attribute only in relation to manure applied at the highest dose. The manure in doses of 100 and 200 m3 ha-1 increased significantly the content of extractable P at depths 0-2 and 4-6 cm, including in relation to soluble fertilizer. At th

O uso do dejeto suíno como fertilizante é a alternativa mais utilizada para o descarte desse resíduo. Entretanto, a dose aplicada não deve ultrapassar a capacidade de adsorção do solo a fim de evitar que o excesso de elementos, especialmente P, Cu e Zn, possa atuar como poluente. O presente estudo foi conduzido com o objetivo de avaliar as variações nos atributos químicos de um Latossolo Vermelho distroférrico provocadas por seis aplicações anuais de dejeto suíno (DJ), nas doses 0 (testemunha), 25, 50, 100 e 200 m3 ha-1, de adubo solúvel (AS), e de dejeto complementado com adubo solúvel (DJ+AS). O experimento foi conduzido a campo, no município de Campos Novos, SC, no período de 2001 a 2007. Anualmente, foram cultivados milho (Zea mays) e aveia (Avena strigosa) em cultivos sucessivos, no sistema plantio direto. Em agosto de 2007, o solo foi amostrado nas profundidades de 0-2, 4-6, 9-11, 19-21, 29-31 e 39-41 cm. O DJ não afetou o pH e os teores de Al e Mg trocáveis do solo, mas aumentou o teor de Ca trocável, embora somente na dose 200 m3 ha-1 e na profundidade 0-2 cm. Nesta, o AS diminuiu o pH em relação aos demais tratamentos, tendo também diminuído este atributo na profundidade 4-6 cm, porém, somente em relação ao DJ na maior dose aplicada. O DJ, em doses de 100 e 200 m3 ha-1, aumentou o teor de P extraível nas camadas de 0-2 e 4-6 cm, inclusive em relação ao AS. Na maior dos