ABSTRACT
Objective To determine the chlorpyrifos in human blood by liquid chromatography-tandemmass spectrometry and to validate its application in poisoning cases. Methods The samples were extracted by a simple one-step protein precipitation procedure. Chromatography was performed on a Capcell Pack C18 mG II column (250 mm×2.0 mm, 5μm) using an isocratic elution of solvent A (0.1% formic acid-water with 2 mmol/L ammoniumacetate) and solvent B (methanol with 2 mmol/L ammoniumacetate) at 5∶95 (V∶V).Results The linearranged from5 to 500ng/mL (r=0.9987).Thelimitofdetection (LOD) and the lower limit of quantification (LLOQ ) were 2 ng/mL and 4 ng/mL , respectively. For this method, the precision and accuracy of intra-day and inter-day were <10% and 97.44%-101.10%, respectively. The re-sults in stability test of long-termfrozen were satisfied. The matrix effect, recovery and process efficien-cy were 64.97%-86.81%, 76.70%-85.52%, and 55.57%-66.58%, respectively. Conclusion This method can provide a rapid approach to chlorpyrifos extraction and determination in toxicological analysis of forensic and clinical treatment.
ABSTRACT
Two trials of external quality assessment for the newborn screening tests of inborn errors of metabolism including PKU screening, TSH, T4 (total/free), galactosemia screen, maple syrup urine disease screen and homocytinuria screen, were performed in 2007. Total 20 specimens for the newborn screening tests were distributed to 16 laboratories. The response rate was 100%. All the control materials were sent as filter paper forms. The mean, SD, CV, median and range were analyzed. From this trial, the proficiency tests for newborn screening tests using tandem mass spectrometry (MS/MS) were included as routine quality assessment. Participants results for amino acids, acylcarnitine and their cutoff values were analyzed. In addition, the trial of external quality assessment for glycohemoglobin (HbA1c) was first started in 2007. Two samples prepared from normal whole blood and commercially available abnormal control materials, were distributed to 246 laboratories and the response rate was 93%. Statistical data such as mean, SD and CV were provided for peer groups.
Subject(s)
Humans , Infant, Newborn , Amino Acids , Carnitine , Galactosemias , Korea , Maple Syrup Urine Disease , Mass Screening , Metabolism, Inborn Errors , Peer Group , Tandem Mass SpectrometryABSTRACT
Two trials of external quality assessment for the newborn screening tests of inborn errors of metabolism including PKU screening, TSH, T4 (total/free), galactosemia screen, maple syrup urine disease screen and homocytinuria screen, were performed in 2007. Total 20 specimens for the newborn screening tests were distributed to 16 laboratories. The response rate was 100%. All the control materials were sent as filter paper forms. The mean, SD, CV, median and range were analyzed. From this trial, the proficiency tests for newborn screening tests using tandem mass spectrometry (MS/MS) were included as routine quality assessment. Participants results for amino acids, acylcarnitine and their cutoff values were analyzed. In addition, the trial of external quality assessment for glycohemoglobin (HbA1c) was first started in 2007. Two samples prepared from normal whole blood and commercially available abnormal control materials, were distributed to 246 laboratories and the response rate was 93%. Statistical data such as mean, SD and CV were provided for peer groups.
