Regulation and mechanistic insights into tensile strain in mesenchymal stem cell osteogenic differentiation.

Bone marrow mesenchymal stem cells (BMSCs) are integral to bone remodeling and homeostasis, as they are capable of differentiating into osteogenic and adipogenic lineages. This differentiation is substantially influenced by mechanosensitivity, particularly to tensile strain, which is a prevalent mechanical stimulus known to enhance osteogenic differentiation. This review specifically examines the effects of various cyclic tensile stress (CTS) conditions on BMSC osteogenesis. It delves into the effects of different loading devices, magnitudes, frequencies, elongation levels, dimensionalities, and coculture conditions, providing a comparative analysis that aids identification of the most conducive parameters for the osteogenic differentiation of BMSCs. Subsequently, this review delineates the signaling pathways activated by CTS, such as Wnt/ß-catenin, BMP, Notch, MAPK, PI3K/Akt, and Hedgehog, which are instrumental in mediating the osteogenic differentiation of BMSCs. Through a detailed examination of these pathways, this study elucidates the intricate mechanisms whereby tensile strain promotes osteogenic differentiation, offering valuable guidance for optimizing therapeutic strategies aimed at enhancing bone regeneration.

Grain-Boundary-Rich RuO2 Porous Nanosheet for Efficient and Stable Acidic Water Oxidation.

RuO2 has been considered as the most likely acidic oxygen evolution reaction (OER) catalyst to replace IrO2, but its performance, especially long-term stability under harsh acidic conditions, is still unacceptable. Here, we propose a grain boundary (GB) engineering strategy by fabricating the ultrathin porous RuO2 nanosheet with abundant of grain boundaries (GB-RuO2) as an efficient acid OER catalyst. The involvement of GB induces significant tensile stress and creates an unsaturated coordination environment, effectively optimizing the adsorption of intermediates and stabilizing active site structure during OER process. Notably, the GB-RuO2 not only exhibits a low overpotential (η10=187 mV) with an ultra-low Tafel slope (34.5 mV dec-1), but also steadily operates for over 550 h in 0.1 M HClO4. Quasi in situ/operando methods confirm that the improved stability is attributed to GB preventing Ru dissolution and greatly inhibiting the lattice oxygen oxidation mechanism (LOM). A proton exchange membrane water electrolysis (PEMWE) using the GB-RuO2 catalyst operates a low voltage of 1.669 V at 2 A cm-2 and operates stably for 100 h at 100 mA cm-2.

A triple-crosslinking strategy for high-performance regenerated cellulose fibers derived from waste cotton textiles.

Regenerated cellulose fibers has attracted increasing attention for high-grade textile raw materials and industrial textiles, but the low mechanical property caused by differences in regenerated raw materials and production levels limits its commercial application in the product diversity. Herein, we proposed a novel triple-crosslinking strategy by coupling with hydrogen bonds, chemical crosslinking, and internal mineralization from multiple pulsed vapor phase infiltration (MPI) to improve the mechanical performance of regenerated cellulose fibers. A binary solvent composed of ionic liquid (IL) and dimethyl sulfoxide (DMSO) is used to dissolve waste cotton textile and then wet spinning. Dual-crosslinking is firstly achieved by coupling glutaraldehyde (GA) and cellulose reaction. Subsequently, a metal oxide is intentionally infiltrated into inner cellulosic through MPI technology to form a third form of crosslinking, accompanied by the ultra-thin metal oxide nano-layer onto the surface of regenerated cellulose fibers. Results showed that the triple-crosslinking strategy has increased the tensile stress of the fiber by 43.57 % to 287.03 MPa. In all, triple-crosslinking strategy provides a theoretical basis and technical approach for the reinforcement of weak fibers in waste cotton recycling, which is expected to accelerate the development of the waste textile recycling industry and promote of the added-value of regenerated products.

Directional fracturing of rock by soundless chemical demolition agents.

Rock fracturing is one of the main processes in the modern mining, mineral recovery, and energy industry, which is associated with the numerous environmentally harmful influences. Environmentally friendly alternative to conventional fracturing methods is non-explosive technology, which based on using of soundless chemical demolition agents (SCDA). In this study, method of directional fracturing, which based on creating tensile stress concentrators on the hole surface in the required location, was proposed. Considering the influences of additional installation in the borehole of stress concentration cartridge on stress field nearby the hole, a case study of the effectiveness of proposed rock fracturing method was performed in this paper. A numerical simulation was used to study the stress distribution around the borehole with SCDA in different rock types. It was found that cartridge installation leads to the occurrence of the tensile stress concentrations only near to the gap of the cartridge. The value of tensile stress concentration in the proposed rock fracturing method significantly depends on a cartridge gap distance. An increase in the gap distance leads to a decrease in the stress concentration factor. For granite, sandstone and mudstone, relationships of stress concentration factor to cartridge gap distance that was divided on hole diameter were obtained. The equation for calculating of the hole spacing for the directional fracturing method was proposed. The results of the study are confirmed by experiments in-situ on the directional fracturing of rocks in a quarry and in a coal mine.

Scutellarin alleviates tensile stress-induced proliferation and migration of venous smooth muscle cells via mediating the p38 MAPK pathway.

OBJECTIVE: Abnormal proliferation and migration of biomechanical force-induced venous smooth muscle cells (VSMCs) is a major cause to limit the efficacy of coronary artery bypass grafting (CABG) for coronary heart disease (CHD). Scutellarin is the main active ingredient of Erigeron Breviscapus, and has broad-spectrum pharmacological effects. Therefore, the present study was proposed to investigate the effect of Scutellarin on VSMCs under tensile stress. METHODS: After interfering with VSMCs at different tensile stresses, the optimal tensile stress was screened. In a tensile stress environment, 100 µM Scutellarin and Hesperetin (p38 MAPK pathway activator) was used to treatment with VSMCs. CCK-8, EDU, Wound healing, flow cytometry and western blotting assays were used to detect cell proliferation, migration, apoptosis, and the expression of apoptosis-related proteins (Caspase3, Bcl2 and Bax). RESULTS: Tensile stress with 10% significantly enhanced the activity, wound-healing ratio, and EDU+ cells of VSMCs, and decreased their apoptosis ratio. Moreover, it upregulated Bcl2 expression, and downregulated cleaved-Caspase3 and Bax expression of VSMCs. Hence, 10% tensile stress was selected to creates a tensile stress environment for VSMCs. Interestingly, 100 µM Scutellarin alleviated the effect of 10% tensile stress on the phenotype of VSMCs. Notably, 10% tensile stress increased the phosphorylation level of p38 MAPK (Thr180 +Tyr182) in VSMCs, which was restricted by Scutellarin. Further, Hesperetin restored the effect of Scutellarin on the phenotype of VSMCs. CONCLUSION: Scutellarin alleviates tension stress-induced proliferation and migration of VSMCs via suppressing p38 MAPK pathway. Scutellarin may be used as an adjunctive strategy for future GABG treatment in CHD patients.

Nondestructive Evaluation of Tensile Stress-loaded GFRPs Using the Magnetic Recording Method.

This paper presents the results of inspecting tensile stress-loaded GFRP (glass fiber-reinforced polymer) samples using the Magnetic Recording Method (MRM). The MRM can be utilized solely to examine ferromagnetic materials. The modification was proposed in order to examine nonmagnetic composites. Ferromagnetic strips made of low-carbon steel DC01 were bonded to the surface using an adhesive composed of epoxy resin with the addition of triethylenetetramine. The modified method's feasibility was tested on six samples made of GFRP. The research procedure consisted of three steps. In the first step, a metal strip is glued at the top surface of each sample, and an array of 100 cylindrical permanent magnets is used to record a sinusoidal magnetic pattern on the strip. The initial residual magnetization is measured in the second step, and the samples are subjected to static stress. In the third step, the residual magnetization is measured one more time. Ultimately, the measurement results from the second and third steps are compared. Generally, the applied stress causes changes in the amplitude and frequency of the sinusoidal magnetization pattern. In the case of GFRP, the frequency changes have not been used for evaluation due to minimal variations. The statistical parameters (mean, median, max, and mode) of the RMS (root mean square) value of the sinusoidal pattern were calculated and analyzed. The analysis demonstrates that the modified method is suitable for providing unequivocal and exact information on the load applied to a nonmagnetic composite material. For the presented results, the applied load can be assessed unambiguously for the samples elongated up to 0.6%.

Determining optimal length of coracoid graft in the modified Bristow procedure for anterior shoulder instability: A three-dimensional finite element analysis.

BACKGROUND: There is a lack of consensus concerning the coracoid graft length in the modified Bristow procedure. OBJECTIVE: We attempted to determine the optimal graft length using the three-dimensional finite element method. METHODS: In a shoulder model with a 25% anterior glenoid defect, a coracoid graft of varying lengths (5, 10, 15, and 20 mm) was fixed using a half-threaded screw. First, a compressive load of 500 N was applied to the screw head to determine the graft failure load during screw tightening. Next, a tensile load (200 N) was applied to the graft to determine the failure load due to biceps muscle traction. RESULTS: In the screw compression, the failure loads in the 5-, 10-, 15-, and 20-mm models were 252, 370, 377, and 331 N, respectively. In the tensile load applied to the coracoid graft, the failure load exceeded 200 N for both the 5- and 10-mm models. CONCLUSION: The 5-mm graft had a high risk of fracture during intraoperative screw tightening. As for the biceps muscle traction, the 5- and 10-mm-grafts had a lower failure risk than the 15- and 20-mm-grafts. Therefore, we believe that the optimal length of the coracoid graft is 10 mm in the modified Bristow procedure.

Bio-inspired based meta-heuristic approach for predicting the strength of fiber-reinforced based strain hardening cementitious composites.

A recently introduced bendable concrete having hundred times greater strain capacity provides promising results in repair of engineering structures, known as strain hardening cementitious composites (SHHCs). The current research creates new empirical prediction models to assess the mechanical properties of strain-hardening cementitious composites (SHCCs) i.e., compressive strength (CS), first crack tensile stress (TS), and first crack flexural stress (FS), using gene expression programming (GEP). Wide-ranging records were considered with twelve variables i.e., cement percentage by weight (C%), fine aggregate percentage by weight (Fagg%), fly-ash percentage by weight (FA%), Water-to-binder ratio (W/B), super-plasticizer percentage by weight (SP%), fiber amount percentage by weight (Fib%), length to diameter ratio (L/D), fiber tensile strength (FTS), fiber elastic modulus (FEM), environment temperature (ET), and curing time (CT). The performance of the models was deduced using correlation coefficient (R) and slope of regression line. The established models were also assessed using relative root mean square error (RRMSE), Mean absolute error (MAE), Root squared error (RSE), root mean square error (RMSE), objective function (OBF), performance index (PI) and Nash-Sutcliffe efficiency (NSE). The resulting mathematical GP-based equations are easy to understand and are consistent disclosing the originality of GEP model with R in the testing phase equals to 0.8623, 0.9269, and 0.8645 for CS, TS and FS respectively. The PI and OBF are both less than 0.2 and are in line with the literature, showing that the models are free from overfitting. Consequently, all proposed models have high generalization with less error measures. The sensitivity analysis showed that C%, Fagg%, and ET are the most significant variables for all three models developed with sensitiveness index higher than 10 %. The result of the research can assist researchers, practitioners, and designers to assess SHCC and will lead to sustainable, faster, and safer construction from environment-friendly waste management point of view.

Regulation of the integrin αVß3- actin filaments axis in early osteogenic differentiation of human mesenchymal stem cells under cyclic tensile stress.

BACKGROUND: Integrins are closely related to mechanical conduction and play a crucial role in the osteogenesis of human mesenchymal stem cells. Here we wondered whether tensile stress could influence cell differentiation through integrin αVß3. METHODS: We inhibited the function of integrin αVß3 of human mesenchymal stem cells by treating with c(RGDyk). Using cytochalasin D and verteporfin to inhibit polymerization of microfilament and function of nuclear Yes-associated protein (YAP), respectively. For each application, mesenchymal stem cells were loaded by cyclic tensile stress of 10% at 0.5 Hz for 2 h daily. Mesenchymal stem cells were harvested on day 7 post-treatment. Western blotting and quantitative RT-PCR were used to detect the expression of alkaline phosphatase (ALP), RUNX2, ß-actin, integrin αVß3, talin-1, vinculin, FAK, and nuclear YAP. Immunofluorescence staining detected vinculin, actin filaments, and YAP nuclear localization. RESULTS: Cyclic tensile stress could increase the expression of ALP and RUNX2. Inhibition of integrin αVß3 activation led to rearrangement of actin filaments and downregulated the expression of ALP, RUNX2 and promoted YAP nuclear localization. When microfilament polymerization was inhibited, ALP, RUNX2, and nuclear YAP nuclear localization decreased. Inhibition of YAP nuclear localization could reduce the expression of ALP and RUNX2. CONCLUSIONS: Cyclic tensile stress promotes early osteogenesis of human mesenchymal stem cells via the integrin αVß3-actin filaments axis. YAP nuclear localization participates in this process of human mesenchymal stem cells. Video Abstract.

Degradation of Mechanical Properties of A-PET Films after UV Aging.

In 2018, the European Commission adopted the European Strategy for Plastics in a Circular Economy, which outlines key actions to reduce the negative impact of plastic pollution. The strategy aims to expand plastic recycling capacity and increase the proportion of recycled materials in plastic products and packaging. Using recycled plastic can save 50-60% energy compared to virgin plastic. Recycled PET can be used in the production of A-PET films, which are predominantly used in thermo-vacuum forming for food packaging. Storage conditions can influence the mechanical properties of polymer materials. This work presents changes in the mechanical properties of A-PET films after UV irradiation. An experimental investigation of the UV aging of A-PET films was conducted in a UV aging chamber. The specimens were exposed to a UV radiation dose rate of 2.45 W/m2 for 1, 2, 4, 8, 16, 24, 32, and 40 h. UV measurements were also taken on a sunny day to compare the acceleration of UV irradiation in the UV aging chamber. Mechanical tensile tests were performed on two different three-layer A-PET films (100% virgin and 50% recycled). The tensile strength and relative elongation of the A-PET films were determined, and the work required to break the film was calculated. The total consumed work was divided into the work needed for elastic and plastic deformations. A study of the UV aging of A-PET films confirmed a significant effect on the films, including a loss of plasticity even after brief exposure to solar irradiance. The results of the puncture impact test further confirmed the deterioration of the mechanical properties of A-PET material due to exposure to UV radiation, with a greater effect observed for the recycled material.

Multidentate Chelation Achieves Bilateral Passivation toward Efficient and Stable Perovskite Solar Cells with Minimized Energy Losses.

Defects in the electron transport layer (ETL), perovskite, and buried interface will result in considerable nonradiative recombination. Here, a bottom-up bilateral modification strategy is proposed by incorporating arsenazo III (AA), a chromogenic agent for metal ions, to regulate SnO2 nanoparticles. AA can complex with uncoordinated Sn4+/Pb2+ in the form of multidentate chelation. Furthermore, by forming a hydrogen bond with formamidinium (FA), AA can suppress FA+ defects and regulate crystallization. Multiple chemical bonds between AA and functional layers are established, synergistically preventing the agglomeration of SnO2 nanoparticles, enhancing carrier transport dynamics, passivating bilateral defects, releasing tensile stress, and promoting the crystallization of perovskite. Ultimately, the AA-optimized power conversion efficiency (PCE) of the methylammonium-free (MA-free) devices (Rb0.02(FA0.95Cs0.05)0.98PbI2.91Br0.03Cl0.06) is boosted from 20.88% to 23.17% with a high open-circuit voltage (VOC) exceeding 1.18 V and ultralow energy losses down to 0.37 eV. In addition, the optimized devices also exhibit superior stability.

Effect of Dental Glass Fiber Posts on Root Stresses and Fracture Behavior of Endodontically Treated Maxillary Central Incisors: A Finite Element Analysis Study.

OBJECTIVE:  In this work, the influence of glass fiber posts with different designs on the root stress that had endodontic treatment was examined using the finite element method. METHOD:  Using two distinct materials (metal and glass fiber) and two different prototypes (tapered and parallel-sided), four three-dimensional (3D) finite element models of an upper central incisor were made and studied. Each 3D model received an oblique loading of 100 N. All forces were dispatched as distributed pressure to the aforementioned region. There were no considerations made for potential stresses when performing the endodontic procedure. The endodontic treatment was conducted without taking into account any potential stressors. The root stresses were then recorded. RESULTS: The largest tensile stress is often focused at the apical third of the post and post/cement contact, as well as at the coronal third of the root on both the labial and palatal sides of the root, independent of the post's design and material. Restoration of endodontically treated maxillary central incisors with glass fiber posts has been shown to have less stress concentration than titanium posts. Regardless of the post materials employed, the tapered post design generated a higher tensile stress distribution than the parallel side design. CONCLUSIONS:  Prefabricated fiber posts used in model restoration resulted in more evenly distributed stress and less concentrated stress on the root. Reduction in modulus of elasticity of post materials used generally shows less stress concentration.

Hippo-YAP signaling pathway regulates autophagy of human periodontal ligament cells under cyclic tensile stress.

OBJECTIVES: This work aimed to investigate the molecular mechanism of cyclic tensile stress (CTS) stimulating autophagy in human periodontal ligament cells (hPDLCs). METHODS: hPDLCs were isolated and cultured from normal periodontal tissues. hPDLCs were loaded with tensile stress by force four-point bending extender to simulate the autophagy of hPDLCs induced by orthodontic force du-ring orthodontic tooth movement. XMU-MP-1 was used to inhibit the Hippo signaling pathway to explore the role of the Hippo-YAP signaling pathway in activating hPDLC autophagy by tensile stress. The expression levels of autophagy-related genes (Beclin-1, LC3, and p62) in hPDLCs were detected by real-time quantitative polymerase chain reaction. Western blot was used to detect the expression levels of autophagy-related proteins (Beclin-1, LC3-Ⅱ/LC3-Ⅰ, and p62) and Hippo-YAP pathway proteins (active-YAP and p-YAP) in hPDLCs. Immunofluorescence was used to locate autophagy-related proteins (LC3-Ⅱand p62) and Hippo-YAP pathway proteins (active-YAP) of hPDLCs. RESULTS: CTS-activated autophagy in hPDLCs and expression of autophagy-related proteins initially increased and then decreased; it began to increase at 30 min, peaked at 3 h, and decreased (P<0.05). CTS increased the expression of active-YAP protein and decreased the expression of p-YAP protein (P<0.05). When XMU-MP-1 inhibited the Hippo-YAP signaling pathway (P<0.05), active-YAP protein was promoted to enter the nucleus and autophagy expression was enhanced (P<0.05). CONCLUSIONS: The Hippo-YAP signaling pathway is involved in the regulation of autophagy activation in hPDLCs under CTS.

Effect of Acetylation of Two Cellulose Nanocrystal Polymorphs on Processibility and Physical Properties of Polylactide/Cellulose Nanocrystal Composite Film.

Polylactide (PLA) has become a popular alternative for petroleum-based plastics to reduce environmental pollution. The broader application of PLA is hampered by its brittle nature and incompatibility with the reinforcement phase. The aim of our work was to improve the ductility and compatibility of PLA composite film and investigate the mechanism by which nanocellulose enhances PLA polymer. Here, we present a robust PLA/nanocellulose hybrid film. Two different allomorphic cellulose nanocrystals (CNC-I and CNC-III) and their acetylated products (ACNC-I and ACNC-III) were used to realize better compatibility and mechanical performance in a hydrophobic PLA matrix. The tensile stress of the composite films with 3% ACNC-I and ACNC-III increased by 41.55% and 27.22% compared to pure PLA film, respectively. Compared to the CNC-I or CNC-III enhanced PLA composite films, the tensile stress of the films increased by 45.05% with 1% ACNC-I and 56.15% with 1% ACNC-III. In addition, PLA composite films with ACNCs showed better ductility and compatibility because the composite fracture gradually transitioned to a ductile fracture during the stretching process. As a result, ACNC-I and ACNC-III were found to be excellent reinforcing agents for the enhancement of the properties of polylactide composite film, and the replacement some petrochemical plastics with PLA composites would be very promising in actual life.

Mechano-Electrochemically Promoting Lithium Atom Diffusion and Relieving Accumulative Stress for Deep-Cycling Lithium Metal Anodes.

Lithium metal batteries (LMBs) can double the energy density of lithium-ion batteries. However, the notorious lithium dendrite growth and large volume change are not well addressed, especially under deep cycling. Here, an in-situ mechanical-electrochemical coupling system is built, and it is found that tensile stress can induce smooth lithium deposition. Density functional theory (DFT) calculation and finite element method (FEM) simulation confirm that the lithium atom diffusion energy barrier can be reduced when the lithium foils are under tensile strain. Then tensile stress is incorporated into lithium metal anodes by designing an adhesive copolymer layer attached to lithium in which the copolymer thinning can yield tensile stress to the lithium foil. Elastic lithium metal anode (ELMA) is further prepared via introducing a 3D elastic conductive polyurethane (CPU) host for the copolymer-lithium bilayer to release accumulated internal stresses and resist volume variation. The ELMA can withstand hundreds of compression-release cycles under 10% strain. LMBs paired with ELMA and LiNi0.8 Co0.1 Mn0.1 O2 (NCM811) cathode can operate beyond 250 cycles with 80% capacity retention under practical condition of 4 mAh cm-2 cathode capacity, 2.86 g Ah-1 electrolyte-to-capacity ratio (E/C) and 1.8 negative-to-cathode capacity ratio (N/P), five times of the lifetime using lithium foils.

Effect of static tensile stress on enzymatic degradation of poly(glycerol sebacate).

Poly(glycerol sebacate) (PGS) is an excellent scaffold material in tissue engineering due to good biocompatibility and tunable mechanical properties. The degradation properties of PGS have been primarily explored in static phosphate buffer solution or enzyme solution. It is vital to understand how the tensile stress affect the degradation rate. In this study, PGS was synthetized by melt polycondensation and its properties were characterized. Then an in vitro degradation device which could provide different constant tensile stresses was carefully designed and established, and the enzymatic degradation of PGS was tested under 0-150 kPa at 37°C. It was found that holes of PGS surface arranged almost parallel to each other and perpendicular to the direction of tensile stresses at 100 kPa and 150 kPa after 2-4 days degradation. After 8 days degradation, the ultimate tensile strength (UTS) of PGS at 150 kPa was 0.28 MPa and the elastic modulus was 1.11 MPa, while the UTS of PGS was 0.44 MPa and the elastic modulus was 1.63 MPa before degradation, both of them have significant differences. Hence, the tensile stress and degradation time were proportional to the appear time and size of holes, leading to the decrease of mass loss, UTS and elastic modulus. The relationship between stress and PGS degradation rates was quantitatively described through our degradation experiments, providing guidance for suitable PGS applications in the future.

Cyclic tensile force modifies calvarial osteoblast function via the interplay between ERK1/2 and STAT3.

BACKGROUND: Mechanical therapies, such as distraction osteogenesis, are widely used in dental clinics. During this process, the mechanisms by which tensile force triggers bone formation remain of interest. Herein, we investigated the influence of cyclic tensile stress on osteoblasts and identified the involvement of ERK1/2 and STAT3. MATERIALS AND METHODS: Rat clavarial osteoblasts were subjected to tensile loading (10% elongation, 0.5 Hz) for different time periods. RNA and protein levels of osteogenic markers were determined using qPCR and western blot after inhibition of ERK1/2 and STAT3. ALP activity and ARS staining revealed osteoblast mineralization capacity. The interaction between ERK1/2 and STAT3 was investigated by immunofluorescence, western blot, and Co-IP. RESULTS: The results showed that tensile loading significantly promoted osteogenesis-related genes, proteins and mineralized nodules. In loading-induced osteoblasts, inhibition of ERK1/2 or STAT3 decreased osteogenesis-related biomarkers significantly. Moreover, ERK1/2 inhibition suppressed STAT3 phosphorylation, and STAT3 inhibition disrupted the nuclear translocation of pERK1/2 induced by tensile loading. In the non-loading environment, inhibition of ERK1/2 hindered osteoblast differentiation and mineralization, while STAT3 phosphorylation was elevated after ERK1/2 inhibition. STAT3 inhibition also increased ERK1/2 phosphorylation, but did not significantly affect osteogenesis-related factors. CONCLUSION: Taken together, these data suggested that ERK1/2 and STAT3 interacted in osteoblasts. ERK1/2-STAT3 were sequentially activated by tensile force loading, and both affected osteogenesis during the process.

Approaching the Fill Factor Limit in Dopant-Free Hole Transporting Layer-Based All-Inorganic Perovskite Solar Cells.

As an important part of perovskite solar cells (PSCs), hole transporting layer (HTL) has a critical impact on the performance and stability of the devices. In an attempt to alleviate the moisture and thermal stability issues from the commonly used HTL Spiro-OMeTAD with dopant, it is urgent to develop novel HTLs with high stability. In this study, a new class of polymers D18 and D18-Cl are applied as undoped HTL for CsPbI2Br-based PSCs. In addition to the excellent hole transporting properties, we unveil that D18 and D18-Cl with larger thermal expansion coefficient than that of CsPbI2Br could impose a compressive stress onto the CsPbI2Br film upon thermal treatment, which could release the residual tensile stress in the film. As a result, the efficiency of CsPbI2Br-based PSCs with D18-Cl as HTL reaches 16.73%, and the fill factor (FF) exceeds 85%, which is one of the highest FF records for the conventional-structured device to date. The devices also show impressive thermal stability with over 80% of the initial PCE retained after 85 °C heating for 1500 h.

Size and proximity of micro-scale hard-inclusions increase the risk of rupture in fibroatheroma-like laboratory models.

Increased mechanical stresses of the fibroatheroma cap tissue is a crucial risk factor on the pathogenesis of asymptomatic coronary artery disease events. Moreover, both numerical and analytical studies have shown that microcalcifications (µCalcs) located in the fibrous cap can multiply the cap tissue stress by a factor of 2-7. This stress amplification depends on the ratio of the gap between particles (h) and their diameter (D) when they are aligned along the tensile axis. However, the synergistic effect of cap stiffness and uCalcs on the ultimate stress and rupture risk of the atheroma cap has not been fully investigated. In this context, we studied the impact of micro-beads (µBeads) of varying diameters and concentration on the rupture of silicone-based laboratory models mimicking human fibroatheroma caps of different stiffness (shear moduli µsoft = 40 kPa, µstiff = 400 kPa) and thickness (650 µm and 100 µm). A total of 145 samples were tested under uniaxial tension up to failure and the true stress and strain response of each model was derived by means of Digital Image Correlation (DIC). Before testing, samples were scanned using high-resolution Micro-CT, to perform morphometry analyses of the embedded micro-beads and determine the number of closely spaced particles (h/D<0.5). The micro-beads structural and spatial features were then compared to the case of 29 non-ruptured human atheroma fibrous caps presenting µCalcs. Samples with and without µBeads exhibited a distinct hyperelastic behavior typical of arterial tissues. Regardless of the sample stiffness, large µBeads (>80 µm) significantly reduced the ultimate tensile stress (UTS) of the thick cap models with the effect being more pronounced as the particle diameter increases. Stiff models experienced early rupture in the presence of µBeads with 40 µm diameter. Smaller µBeads of 6 µm and 20 µm didn't affect the ultimate strength of the thick cap models. However, when 6 µm µBeads where introduced in thinner cap models, we observed more than 20% drop in UTS. Increasing the µBeads concentration was also positively correlated with lower stresses at rupture as more clusters formed resulting in lower values of h/D. Morphometry analyses of cap models and human atheroma show that the 6 µm µBeads groups present very similar size distributions to µCalcs and that human µCalcs occupy an average volume ratio of 0.79 ± 0.85%. Our results clearly capture the influence of µBeads on the rupture threshold of a vascular tissue mimicking material. This effect appears to be dependent on the µBeads-to-cap thickness size ratio as well as their proximity. These findings support previous numerical and analytical studies suggesting that µCalcs located within the fibroatheroma cap may be responsible for significantly increasing the risk of cap rupture that precedes myocardial infarction and sudden death.

Micromechanical BEoL robustness evaluation methods enabling loading condition customization and acoustic emission damage monitoring.

For micromechanical robustness evaluation methods, it is advantageous if the mechanical loading conditions applied can be controlled as precisely as possible. For microchips, this is required to determine the robustness under specific conditions, e.g. during assembly or characteristic application/usage scenarios. In this work, three different micromechanical BEoL (Back End of Line) robustness evaluation methods are presented which should enable a more precise and flexible mechanical load induction and damage identification. They have been subsequently developed. Three main aspects characterize the customization of the developed approaches:•The design and testing of customized micro-tools to precisely apply mechanical load to individual Cu-pillars.•The implementation of an AE (Acoustic Emission) monitoring approach to detect minor damages during mechanical loading. This strategy also enabled the development of sub-critical loading experiments for which AE signals served as a damage indicator and mechanical loading was aborted upon the detection of AE events.•The development of a new measurement setup and approach to enable the solder attach of individual Cu-pillars to a mechanical testing system. The applications of these approaches should enable the induction of customized mechanical loading conditions and the identification of failure modes and damage initiation locations.