Textile azo dye, Sudan Black B, inducing hepatotoxicity demonstrated in in vivo zebrafish larval model.

Environmental pollution, particularly from textile industry effluents, raises concerns globally. The aim of this study is to investigate the hepatotoxicity of Sudan Black B (SBB), a commonly used textile azo dye, on embryonic zebrafish. SBB exposure led to concentration-dependent mortality, reaching 100% at 0.8 mM, accompanied by growth retardation and diverse malformations in zebrafish. Biochemical marker analysis indicated adaptive responses to SBB, including increased SOD, CAT, NO, and LDH, alongside decreased GSH levels. Liver morphology analysis unveiled significant alterations, impacting metabolism and detoxification. Also, glucose level was declined and lipid level elevated in SBB-exposed in vivo zebrafish. Inflammatory gene expressions (TNF-α, IL-10, and INOS) showcased a complex regulatory interplay, suggesting an organismal attempt to counteract pro-inflammatory states during SBB exposure. The increased apoptosis revealed a robust hepatic cellular response due to SBB, aligning with observed liver tissue damage and inflammatory events. This multidimensional study highlights the intricate web of responses due to SBB exposure, which is emphasizing the need for comprehensive understanding and targeted mitigation strategies. The findings bear the implications for both aquatic ecosystems and potentially parallels to human health, underscoring the imperative for sustained research in this critical domain.

Biodegradation of crystal violet by newly isolated bacteria.

Confronting the environmental threat posed by textile dyes, this study highlights bioremediation as a pivotal solution to mitigate the impacts of Crystal Violet, a widely-utilized triphenylmethane dye known for its mutagenic and mitotic toxicity. We isolated and identified several bacterial strains capable of degrading Crystal Violet under various environmental conditions. Newly identified strains, including Mycolicibacterium nivoides, Chryseobacterium sp., Agrobacterium rhizogenes, Pseudomonas crudilactis, and Pseudomonas koreensis demonstrated significant decolorization activity of Crystal Violet, complementing the already known capabilities of Stenotrophomonas maltophilia. Initial experiments using crude extracts confirmed their degradation potential, followed by detailed studies that investigated the impact of different pH levels and temperatures on some strains' degradation efficiency. Depending on the bacteria, the degree of activity change according to pH and temperature was different. At 37 °C, Chryseobacterium sp. and Stenotrophomonas maltophilia exhibited higher degradation activity compared to 25 °C, while Pseudomonas crudilactis and Mycolicibacterium nivoides did not exhibit a statistically significant difference between the two temperatures. Mycolicibacterium nivoides performed optimally at pH 8, while Pseudomonas crudilactis showed high activity at pH 5. Stenotrophomonas maltophilia's activity remained consistent across the pH range. These findings not only underscore the effectiveness of these bacteria as agents for Crystal Violet degradation but also pave the way for their application in large-scale bioremediation processes for the treatment of textile effluents, marking them as vital to environmental sustainability efforts.

Effect of co-cultivation of white and brown rot species on basidiome production, lignocelluloytic enzyme activity and dye decolourisation.

The aim of this work was studying the impact of co-cultivating two mushroom species: a white (Pleurotus albidus CLA 45) and a brown rot one (Laetiporus sulphureus BAFC 205) in substrates based on poplar or pine sawdust, on their lignocellulolytic enzyme production, yield values and basidiomes properties. Laetiporus sulphureus only developed basidiome primordia, but P. albidus monoculture and co-culture in pine sawdust achieved biological efficiencies of up to 50-55 %. Co-cultivation on diverse substrates rendered varied enzyme titers. Laccase and Manganese peroxidase titers were highest in pine co-culture and P. albidus poplar monoculture, respectively. Enzymatic extracts obtained from spent poplar substrate of dual cultures displayed potential for treating non-sterile textile-coloured effluents, achieving 35 % decolourisation after 120 h. The knowledge available on the effects of co-culture of white and brown rot fungi is still limited. This study represents an initial exploration of the interaction between them within intensive cultivation conditions.

Microalgae-Based Remediation of Real Textile Wastewater: Assessing Pollutant Removal and Biomass Valorisation.

The textile industry generates highly contaminated wastewater. It severely threatens local ecosystems without proper treatment, significantly diminishing biodiversity near the discharge point. With rapid growth rates, microalgae offer an effective solution to mitigate the environmental impact of textile wastewater, and the generated biomass can be valorised. This study sets out to achieve two primary objectives: (i) to assess the removal of pollutants by Chlorella vulgaris from two distinct real textile wastewaters (without dilution) and (ii) to evaluate microalgal biomass composition for further valorisation (in a circular economy approach). Microalgae grew successfully with growth rates ranging from 0.234 ± 0.005 to 0.290 ± 0.003 d-1 and average productivities ranging from 78 ± 3 to 112.39 ± 0.07 mgDW L-1 d-1. All cultures demonstrated a significant reduction in nutrient concentrations for values below the legal limits for discharge, except for COD in effluent 2. Furthermore, the pigment concentration in the culture increased during textile effluent treatment, presenting a distinct advantage over conventional ones due to the economic value of produced biomass and pigments. This study underscores the promise of microalgae in textile wastewater treatment and provides valuable insights into their role in addressing the environmental challenges the textile industry poses.

Efficient photoelectrochemical real textile wastewater detoxification using photoanodes of C3N4-BiVO4.

Photoelectrochemical systems utilizing solar energy have garnered significant attention for their sustainability in remediating contaminated water. This study focuses on advancing photoanode development through the utilization of carbon nitrides (C3N4) and bismuth vanadate (BiVO4), two promising semiconductor materials renowned for their efficient electron-hole pair separation leading to enhanced photocatalytic activity. Four distinct materials were synthesized and compared: BiVO4 over C3N4, C3N4 over BiVO4, and pristine BiVO4 and C3N4. Upon electrochemical analysis, the C3N4-BiVO4 heterostructure exhibited the highest photoelectrocatalytic charge transfer constant, mobility, and lifetime of charge carriers. Capitalizing on these exceptional properties, the composite was applied to remove organic matter real effluent from the textile industry. The photoelectrodegradation of the effluent demonstrated substantial removal of Total Organic Carbon (TOC) and the generation of low toxicity degradation products, accompanied by low energy consumption. The compelling results underscore the high potential of the synthesized C3N4-BiVO4 heterostructure for industrial applications, particularly in addressing environmental challenges associated with textile industry effluents.

A review on existing and emerging approaches for textile wastewater treatments: challenges and future perspectives.

This comprehensive review explores the complex environment of textile wastewater treatment technologies, highlighting both well-established and emerging techniques. Textile wastewater poses a significant environmental challenge, containing diverse contaminants and chemicals. The review presents a detailed examination of conventional treatments such as coagulation, flocculation, and biological processes, highlighting their effectiveness and limitations. In textile industry, various textile operations such as sizing, de-sizing, dyeing, bleaching, and mercerization consume large quantities of water generating effluent high in color, chemical oxygen demand, and solids. The dyes, mordants, and variety of other chemicals used in textile processing lead to effluent variable in characteristics. Furthermore, it explores innovative and emerging techniques, including advanced oxidation processes, membrane filtration, and nanotechnology-based solutions. Future perspectives in textile wastewater treatment are discussed in-depth, emphasizing the importance of interdisciplinary research, technological advancements, and the integration of circular economy principles. Numerous dyes used in the textile industry have been shown to have mutagenic, cytotoxic, and ecotoxic potential in studies. Therefore, it is necessary to assess the methods used to remediate textile waste water. Major topics including the chemical composition of textile waste water, the chemistry of the dye molecules, the selection of a treatment technique, the benefits and drawbacks of the various treatment options, and the cost of operation are also addressed. Overall, this review offers a valuable resource for researchers and industry professionals working in the textile industry, pointing towards a more sustainable and environmentally responsible future.

Integrated Fenton-like and ozonation based advanced oxidation processes for treatment of real-time textile effluent containing azo reactive dyes.

The treatment of real-time textile effluent, collected from the Common Effluent Treatment Plant (CETP) of Kerala Industrial Infrastructure Development Corporation (KINFRA) at Kannur (District), Kerala (State), India, have been studied by utilizing the Fenton-like and ozone (O3) based advanced oxidation processes (AOPs). The Fenton-like AOP has been conducted as the pre-treatment of textile effluent involving the activation of persulfate (PS) and hydrogen peroxide (H2O2) as a single and the mixed oxidants by using the Flyash (FA)-Pd composite particles as the activator. The maximum chemical oxygen demand (COD) removal of 84% has been observed for a stand-alone O3 based treatment at an O3 flow rate of 5-6 g h-1. By conducting the pre-treatment of textile effluent with the PS, H2O2, and mixed oxidants (PS and H2O2) based Fenton-like AOPs, the COD removal after an O3 based post-treatment has been observed to be 83, 87, and 93% respectively at an O3 flow rate of 2, 3, and 5 g h-1. Hence, the Fenton-like pre-treatment involving the activation of mixed oxidants has been determined to be the best method for the highest COD removal of real-time textile effluent. The optimum values of initial oxidant-ratio (initial [H2O2]:initial [PS]), initial oxidant-dosage, and ozonation time, for the mixed oxidants based Fenton-like pre-treatment, have been determined to be 3 wt% mM-1, 6:2 wt% mM-1, and 60 min respectively. Under the most optimum conditions, the COD removal has been attributed to the combination of O2•- (for the pre-treatment) and •OOH (for the post-treatment) which possess relatively lower oxidation potential values.

Gum arabic-assisted green synthesis of biocompatible MoS2 nanoparticles for methylene blue photodegradation.

The current work reports the gum arabic-mediated greener synthesis of MoS2 nanoparticles (NPs) and its utilization for the solar light-assisted degradation of methylene blue. Furthermore, the safety analyses were performed on human-beneficial gut bacterium, L. delbrueckii, and human blood cells to confirm the biocompatibility of NPs synthesized. Antioxidant and antimicrobial activities were done to explore their usefulness for biological applications. Sonication and microwave treatment were used to obtain spherical 10-12 nm MoS2 NPs as characterized using high-resolution transmission electron microscopy. FT-IR characterization revealed the occurrence of gum arabic on the NPs surface. The MoS2 NPs exhibited ~ 98% MB degradation within 8 h under direct sunlight exposure. Moreover, the reusability studies have also been evaluated and free radical trapping experiments indicated that superoxide (•O2-) is the dominant active species of the reaction system. Furthermore, 98.89% MB degradation efficiency was observed within 150 min in the case of real textile industry MB effluent samples. Untreated MB inhibited the growth of L. delbrueckii on MRS agar plates, while growth was observed in the case of MoS2 NPs-treated MB samples indicating safety of current MB degradation approach. MoS2 NPs inhibited the growth of E. coli MTCC1698 and S. aureus MTCC 3160 with 26 mm and 21 mm zone of inhibition, respectively. Furthermore, MoS2 NPs have shown antioxidant properties, resulting in 82.3 ± 0.43% of DPPH scavenging activity which was comparable to ascorbic acid (81.6 ± 0.6%), a standard antioxidant molecule. The NPs have not shown any hemolytic activity at 0.0625 and 0.125 mg/mL doses to human blood proving their biocompatible nature. Gum arabic-synthesized biocompatible MoS2 NPs have good potential to treat MB released as waste from the textile industry and other biological applications.

Detoxification and decolorization of complex textile effluent in an enzyme membrane reactor: batch and continuous studies.

There is an urgent need to look for bio-based technologies to address the pollution related to textile dyes in waterbodies. The aim of this study was to evaluate an engineered laccase variant, LCC1-62 of Cyathus bulleri, expressed in recombinant Pichia pastoris, for the decolorization and detoxification of real textile effluent. The partially purified laccase effectively (~60-100%) decolorized combined effluent from different dyeing units at a laccase concentration of 500 U/L at a 50-mL level. Decolorization and detoxification of the combined effluents, from a local textile mill, were evaluated at 0.3 L volumetric level in a ray-flow membrane reactor in batch and continuous modes of operation. In batch studies, maximum decolorization of 97% and detoxification of 96% occurred at a hydraulic retention time (HRT) of 6 h without any additional laccase requirement. In continuous studies, the reactor was operated at an HRT of 6 h with a lower enzyme dosage (~120 U/L of the effluent). Decolorization was accompanied by a loss in laccase activity which was restored to ~120 U/L by the addition of laccase in two regimes. The addition of laccase, when the residual laccase activity decreased to 40% (~50 U/L), resulted in high decolorization (~5 ppm residual dye concentration) and low variance (σ2) of 2.77, while laccase addition, when the residual dye concentration decreased to ~8% (~10 U/L), resulted in an average dye concentration of 13 ppm with a high variance of 62.08. The first regime was implemented, and the continuous reactor was operated for over 80 h at an HRT of 3 and 6 h, with the latter resulting in ~95% decolorization and 96% reduction in the mutagenicity of the effluent. Less than 10% membrane fouling was observed over long operations of the reactor. The findings strongly suggest the feasibility of using LCC1-62 in an enzyme membrane reactor for large-scale treatment of textile effluents.

Life cycle assessment of sequential microbial-based anaerobic-aerobic reactor technology developed onsite for treating textile effluent.

Although biological treatment of textile effluent is a preferred option for industries avoiding toxic chemical sludge production and disposal, requirement of several extra pre-treatment units like neutralization, cooling systems or additives, results in higher operational cost. In the present study, a pilot scale sequential microbial-based anaerobic-aerobic reactor technology (SMAART) was developed and operated for the treatment of real textile effluent in the industrial premises in continuous mode for 180 d. The results showed an average ∼95% decolourization along with ∼92% reduction in the chemical oxygen demand establishing the resilience against fluctuations in the inlet parameters and climate conditions. Moreover, the pH of treated effluent was also reduced from alkaline range (∼11.05) to neutral range (∼7.76) along with turbidity reduction from ∼44.16 NTU to ∼0.14 NTU. A comparative life cycle assessment (LCA) of SMAART with the conventional activated sludge process (ASP) showed that ASP caused 41.5% more negative impacts on environment than SMAART. Besides, ASP had 46.15% more negative impact on human health, followed by 42.85% more negative impact on ecosystem quality as compared to SMAART. This was attributed to less electricity consumption, absence of pre-treatment units (cooling and neutralization) and less volume of sludge generation (∼50%) while using SMAART. Hence, integration of SMAART within the industrial effluent treatment plant is recommended to achieve a minimum waste discharge system in pursuit of sustainability.

Coagulation-flocculation treatment for batik effluent as a baseline study for the upcoming application of green coagulants/flocculants towards sustainable batik industry.

The batik industry has been one of the main family businesses in most of the east-coast region of the Malaysian peninsula for many years. However, appropriate water treatment is still a major challenge for this industry. Stringent laws introduced by the Malaysian authorities and the intention to protect the environment are factors that drive researchers to search for suitable, appropriate, affordable and efficient treatment of batik wastewater. Treatment research on batik wastewater is still lacking and coagulation-flocculation treatment using alum was introduced and chosen as a stepping stone toward the selection of green coagulants. This study aimed to determine the best conditions for alum flocculation-coagulation using a standard jar test method. Four main factors were investigated: alum dosage (0.1-3.5 g/L), pH (4-11), settling time (0.5-24 h) and rapid mixing rate (100-300 rpm). Results obtained were further analysed statistically using SPSS software prior to determining the significant effect of variable changes. From this study, the best conditions for batik wastewater treatment using the flocculation-coagulation process were found to be at alum dosage of 1.5 g/L, pH 8, 4 h settling time and a rapid mixing rate of 100 rpm. Chemical oxygen demand (COD), turbidity, colour and total suspended solids (TSS) were removed by 70.7, 92.2, 88.4 and 100%, respectively, under these conditions. This study showed that batik wastewater can be treated by the coagulation-flocculation process using chemical means of alum. This indicates the need for forthcoming developments in natural-based-coagulant-flocculants toward the sustainability of the batik industry.

Eco-friendly microwave assisted sustainable coloration of silk and wool fabric with Acid Blue 07 dye.

Environment-friendly textile processing is the demand of the current global scenario, where the application of sustainable technologies such as microwave radiation has been gaining fame in all global fields due to their green and human-friendly nature. This study has been conducted to employ sustainable technology such as microwave (MW) rays for dyeing polyamide-based proteinous fabric using Acid Blue 07 dye. The fabric before and after MW treatment for up to 10 min has been dyed using an acid dye solution. Spectrophotometric analysis of the dye solution was performed before and after irradiation at a specific selected level. Using selected dyes and irradiation conditions, a series of 32 experiments using a central composite design has been employed. The shades made at selected conditions of irradiation and dyeing were assessed for colorfastness as per ISO standards. It was observed that for dyeing silk, 55 mL of Acid Blue 07 dye solution containing 1 g/100 mL salt solution at 65 °C for 55 min should be employed after MW treatment for 10 min. In comparison, for dyeing wool, 55 mL of Acid Blue 07 dye solution containing 2 g/100 mL salt solution at 65 °C for 55 min should be employed after MW treatment for 10 min. Physiochemical analysis shows that sustainable tool has not altered the chemical nature of fabric but has modified the fabric surface physically to enhance uptake ability. Colorfastness shows that the shades made have offered good resistance to fade and have given good to excellent ratings on the gray scale.

Sonochemical synthesis of Ce-TiO2 nanocatalyst and subsequent application for treatment of real textile industry effluent.

Treatment of real textile industry effluent using photocatalysis, sonocatalysis, sonophotocatalysis and H2O2 assisted sonophotocatalysis have been studied based on the use of Ce-TiO2 nanocatalyst synthesized using sonochemical co-precipitation method. Characterization studies of the obtained catalyst revealed crystallite size as 1.44 nm with particles having spherical morphology. A shift of the absorption edge to the visible light range was also observed in UV-Vis diffuse reflectance spectra (UV-DRS) analysis. The effects of different operational parameters viz catalyst dose (0.5 g/L-2 g/L), temperature (30 °C-55 °C) and pH (3-12) on the COD reduction were studied. The reduction in the COD was higher at lower pH and the optimum temperature established was 45 °C. It was also elucidated that the required catalyst dose was lesser in combined sonophotocatalysis when compared with individual photocatalysis and sonocatalysis. Combination of processes and addition of oxidants increased the COD reduction with the sonophotocatalytic oxidation combined with H2O2 treatment showing the best results for COD reduction (84.75%). The highest reduction in COD for photocatalysis was only 45.09% and for sonocatalysis, it was marginally higher at 58.62%. The highest reduction in COD achieved by sonophotocatalysis was 64.41%. Toxicity tests coupled with Liquid Chromatography Mass Spectrometry (LC-MS) analysis revealed that there were no additional toxic intermediates added to the system during the treatment. Kinetic study allowed establishing that generalized kinetic model fits the experimental results well. Overall, the combined advanced oxidation processes showed better results than the individual processes with higher COD reduction and lower requirement of the catalyst.

Sequential photo electro oxidation and biodegradation of textile effluent: Elucidation of degradation mechanism and bacterial diversity.

Textile effluent contains a highly toxic and refractory azo dyes. Eco-friendly method for efficient decolorization and degradation of textile effluent is essential. In the present study, treatment of textile effluent was carried through sequential electro oxidation (EO) and photo electro oxidation (PEO) using RuO2-IrO2 coated titanium electrode as an anode and cathode followed by biodegradation. The pre-treatment of textile effluent by photo electro oxidation for 14 h exhibited 92% of decolorization. Subsequent biodegradation of the pre-treated textile effluent enhanced the reduction of chemical oxygen demand to 90%. Metagenomics results exhibited that Flavobacterium, Dietzia, Curtobacterium, Mesorhizobium, Sphingobium, Streptococcus, Enterococcus, Prevotellaand Stenotrophomonas bacterial communities majorly involved in the biodegradation of textile effluent. Hence, integrating sequential photo electro oxidation and biodegradation proposed an efficient and eco-friendly approach for treating textile effluent.

Hybrid electrocoagulation and laccase mediated treatment for efficient decolorization of effluent generated from textile industries.

Enzymatic (laccase mediated) decolorization of dyes remains inefficient for recalcitrant dyes, which can be better handled by electrocoagulation (EC). However, EC is energy intensive and produce large amount of sludge. In light of the same, present study offers a promising solution for the treatment of textile effluent meeting surface discharge norms, using hybridization of enzymatic and electrocoagulation treatment. The findings revealed best color removal (90%) of undiluted (raw) textile effluent (4592 hazen) is achievable by employing EC using zinc-coated iron electrode at current density 25 mA cm-2 followed by partially purified laccase (LT) treatment, and activated carbon (AC) polishing at ambient conditions. Overall, the decolorization performance of Hybrid EC-LT integrated AC approach was 1.95 times better than only laccase treatment. Also, the sludge generation from Hybrid EC-LT integrated AC (0.7 g L-1) was 3.3 times lesser than EC alone (2.1 g L-1). Therefore, the present study recommends Hybrid EC-LT integrated AC could be potential approach to treat complex textile effluent sustainably with lower energy input and waste sludge generation.

In situ treatment of real textile effluent in constructed furrows using consortium of Canna indica and Saccharomyces cerevisiae and subsequent biochemical and toxicity evaluation.

Emerging contaminants removals like dyes and heavy metals from the textile effluent have an immense challenge. The present study focuses on the biotransformation and detoxification of dyes and in situ textile effluent treatment by plants and microbes efficiently. A mixed consortium of perennial herbaceous plant Canna indica and fungi Saccharomyces cerevisiae showed decolorization of di-azo dye Congo red (CR, 100 mg/L) up to 97% within 72 h. Root tissues and Saccharomyces cerevisiae cells revealed induction of various dye-degrading oxidoreductase enzymes such as lignin peroxidase, laccase, veratryl alcohol oxidase and azo reductase during CR decolorization. Chlorophyll a, Chlorophyll b and carotenoid pigments were notably elevated in the leaves of a plant during the treatment. Phytotransformation of CR into its metabolic constituents was detected by using several analytical techniques, including FTIR, HPLC, and GC-MS and its non-toxic nature was confirmed by cyto-toxicological evaluation on Allium cepa and on freshwater bivalves. Mix consortium of plant Canna indica and fungi Saccharomyces cerevisiae efficiently treated textile wastewater (500 L) and reduced ADMI, COD, BOD, TSS and TDS (74, 68, 68, 78, and 66%) within 96 h. In situ textile wastewater treatment for in furrows constructed and planted with Canna indica, Saccharomyces cerevisiae and consortium-CS within 4 days reveals reduced ADMI, COD, BOD, TDS and TSS (74, 73, 75, 78, and 77%). Comprehensive observations recommend this is an intelligent tactic to exploit this consortium in the furrows for textile wastewater treatment.

Role of extremophilic Bacillus cereus KH1 and its lipopeptide in treatment of organic pollutant in wastewater.

An effective biosurfactant producer and extremophiles bacteria, Bacillus cereus KH1, was isolated from textile effluent and the biosurfactant was produced using molasses as the sole carbon source. Growth parameters such as pH, temperature, salinity and concentration of molasses were optimised for decolourising the textile effluent with 24-h incubation. The biosurfactant property of B. cereus KH1 was evaluated based on haemolytic activity, oil displacement technique, drop-collapsing test and emulsification index. The results of the produced biosurfactant showed a positive reaction in haemolytic activity, oil displacement technique, drop-collapsing test and exhibiting a 67% emulsification index. The cell-free broth was stable in 40 °C pH 7, 7% salinity and 7% molasses. Thin-Layer Chromatography and Fourier Transform Infrared Spectroscopy analysis revealed that the biosurfactant was a lipopeptide with a yield 2.98 g L-1. These findings proved the synergistic action of B. cereus KH1 with lipopeptide biosurfactant may accelerated the decolourisation efficiency to 87%.

Eco-friendly degradation of reactive red 195, reactive blue 214, and reactive yellow 145 by Klebsiella pneumoniae MW815592 isolated from textile waste.

The water is used in many textile manufacturing steps beyond cleaning. The quantity and the significant chemical load of the effluents generated constitute the primary challenge of the textile industry. In order to discover new sustainable methods to overcome this problem, the aim of this research was to study the potential for degradation of Reactive Blue 214, Reactive Red 195, and Reactive Yellow 145 using a dye degrading bacterium. Sequencing analysis reveals it to be Klebsiella pneumoniae MW815592. This strain completely decolorized artificial effluent (200 mg/L) after 42 h at pH 9 and 46 °C. The decolorization rate increased in the presence of glucose and yeast extract (2 g). In addition, our finding revealed that the decolorization is due to biodegradation rather than adsorption on the bacterial surface.

Development and operation of immobilized cell plug flow bioreactor (PFR) for treatment of textile industry effluent and evaluation of its working efficiency.

The release of untreated/partially treated effluent and solid waste from textile dyeing industries, having un-reacted dyes, their hydrolysed products and high total dissolved solids (TDS) over the period of time had led to the deterioration of ecological niches. In an endeavour to develop a sustainable and effective alternative to conventional approaches, a plug flow reactor (PFR) having immobilized cells of consortium of three indigenous bacterial isolates was developed. The reactor was fed with effluent collected from the equalization tank of a textile processing unit located near city of Amritsar, Punjab (India). The PFR over a period of 3 months achieved 97.98 %, 82.22 %, 87.36%, 77.71% and 68.75% lowering of colour, chemical oxygen demand (COD), biological oxygen demand (BOD), total dissolved solids (TDS) and total suspended solids (TSS) respectively. The comparison of the phytotoxicity and genotoxicity of untreated and PFR-treated output samples using plant and animal models indicated significant lowering of respective toxicity potential. This is a first report, as per best of our knowledge, regarding direct treatment of textile industry effluent without any pre-treatment and with minimal nutritional inputs, which can be easily integrated into already existing treatment plant. The successful implementation of this system will lower the cost of coagulants/flocculants and also lowering the sludge generation.

A novel synergistic effect of TiO2 and ZnO incorporation in PES-based thin-film nanocomposite nanofiltration membrane for treatment of textile wastewater.

A novel synergistic effect of TiO2 and ZnO incorporation in the PES-based thin-film nanocomposite nanofiltration membranes was developed for the treatment of common effluent treatment plant (CETP) textile wastewater. PES@TiO2 membranes were developed by phase inversion via the immersion precipitation method followed by the addition of zinc oxide nanoparticles prepared by the rapid microwave-assisted hydrothermal process via interfacial polymerization. p-Phenylenediamine was used as a monomer for the IP process that was coated on the PES@TiO2 support layer. Various techniques have been applied to characterize the developed thin-film nanocomposite membranes such as Fourier transform infrared (FTIR) microscopy, field emission scanning electron microscopy (FESEM), X-ray powder diffraction (XRD), and contact angle measurement to examine the presence of vibrational modes, surface morphology, the crystal structure of nanoparticles, and hydrophilicity of the membrane, respectively. Membrane properties include porosity, salt rejection, mean pore radius, pure water flux, and industrial effluent rejection efficiency that were studied. The thin-film nanocomposite membrane T5-PES@TiO2(2%)-ZnO(0.3%) was prepared with a combination of 17 wt% PES, 78 wt% DMF, 3 wt% PVP K30, 2% TiO2, 2.5 wt% PPD, 0.3 wt% ZnO, and 1.0 wt% TMC that exhibited high water permeability, porosity, higher industrial effluent rejection, and salt rejection efficiency compared to the neat PES membrane.