ABSTRACT
Objective:To investigate the effect of different dose of Realgar compatible with Indigo Naturalis on the transitional constituents of Indigo Naturalis in rat serum based on the compatibility of Qinghuangsan.Method:Indigo Naturalis test solution, the drug-containing serum of three different proportions of Qinghuangsan (10 g of Indigo Naturalis compatible with 52.5, 105, 210 mg of Realgar for group A, B and C, respectively) and blank serum were detected by UPLC-Q-TOF-MS/MS, in combination with the chemical components identified in Indigo Naturalis test solution, the differences of transitional constituents of Indigo Naturalis in rat serum from the group A, B and C were analyzed. HL-60 cells (human leukemia cells) were treated with the three groups of Qinghuangsan drug-containing serum and the effect of drug-containing serum on the activity of HL-60 cells was detected by cell counting kit-8 (CCK-8) assay.Result:A total of 19, 22, 25 of transitional constituents were detected in Qinghuangsan drug-containing serum from group A, B and C, respectively. The three groups of drug-containing serum all contained 5 prototype components from Indigo Naturalis test solution, including tryptanthrin, indigo, indirubin, 2-aminobenzoic acid and N-phenyl-2-naphthylamine, respectively. The results of CCK-8 assay showed that Qinghuangsan drug-containing serum of group C had the strongest inhibitory effect on HL-60 cells.Conclusion:After fixed Indigo Naturalis dose, with the increase of Realgar dose, the transitional constituents in rat serum increase and the inhibitory effect on HL-60 cells also gradually enhances, which indicates that Realgar may promote the absorption of active components in Indigo Naturali in vivo, thus enhance the efficacy, further explains the compatibility law and pharmacodynamic material basis of different proportions of Realgar and Indigo Naturalis.
ABSTRACT
Objective To conduct serum pharmacochemistry study onShijing pill. Methods HPLC fingerprints of serum in rats after takingShijingpill were established,the serun samples after takingShijing pill, decoction without one of the component drugs and single crude drug were compared,the transitional constituents absorbed into the blood and the original crude after takingShijing pill was determined.Results Fourteen transitional constituents were detected in rat blood after takingShijing pill,four of which were permanent component of blood,seven of which were prototype constituents,three of which were metabolites. Conclusions Some of chemical composition ofShijing pill was clarified preliminarily,that contributes to further studying pharmacodynamic meterial foundation ofShijing pill.
ABSTRACT
Objective: To study the serum pharmacochemistry of leaves of Acanthopanax senticosus. Methods: Rats were ig administered A. senticosus extracts and then the serum was colleted. Rapid identification of transitional constituents absorbed into blood was carried out by UPLC-Q-TOF-MS/MS. The transitional constituents were identified and speculated by Peakview and MetabolitePilot data processing software using retention time, exact relative molecular mass, and cleavage fragments of MS/MS as indexes. Results: After administration of the leaves of A. Senticosus, 19 transitional constituents absorbed into blood were detected in serum, nine of them were prototype constituents and the other 10 were metabolites. Conclusion: Transitional constituents of the leaves of A. senticosus absorbed into blood are preliminarily identified, which could clarify its pharmacodynamic material basis.
ABSTRACT
Objective To conduct serum pharmacochemistry study onShijing pill. Methods HPLC fingerprints of serum in rats after takingShijingpill were established,the serun samples after takingShijing pill, decoction without one of the component drugs and single crude drug were compared,the transitional constituents absorbed into the blood and the original crude after takingShijing pill was determined.Results Fourteen transitional constituents were detected in rat blood after takingShijing pill,four of which were permanent component of blood,seven of which were prototype constituents,three of which were metabolites. Conclusions Some of chemical composition ofShijing pill was clarified preliminarily,that contributes to further studying pharmacodynamic meterial foundation ofShijing pill.
ABSTRACT
Objective: To investigate the bioactive constituents in the stem bark of Eucommia ulmoides and study on their serum pharmacchemistry. Methods: The UHPLC-Q-TOF-MS was used to analyze the ingredients in the serum samples in rats, and the chromatogram was compared among the peaks of extracts in the stem bark of E. ulmoides, rat serum with drug and blank serum sample was used to determine them after the administration of extracts by comparing the finger-print. Results: Seven compounds absorbed into blood were be detected, and had the same retention time with those in the finger-print of extracts, after repeated experiments, which were original constituents of the extracts. The extracts were identified by comparing the retention times with standard substance, five compounds were obtained and identified as geniposidic acid, protocatechuic acid, geniposide, pinoresinol diglucoside, and pinoresinol monoglucoside. Through review of the literature, two compounds may be 1-hydroxypinoresinol glucoside, and eucommiol. Conclusion: The compounds are absorbed into the blood are the effective constituents, and the research provides a scientific fundament for material basis of effectiveness of E. ulmoides.
ABSTRACT
Objective: To carry out a preliminary study on serum parmacochemistry of Dahuang Fuzi Decoction (DFD) and to research the pharmacodynamic material basis through analyzing the constituents absorbed into blood. Methods: HPLC fingerprints of DFD and serum of rats after DFD treatment were established with serum pharmacochemistry method. The serum samples collected after DFD and single crude drug treatments were compared, the constituents absorbed into the serum after DFD ig administration was determined. Results: After the rats were ig administered with DFD, 18 transitional constituents to blood were detected, among which 14 were metabolites and 4 were prototype constituents. Conclusion: After DFD treatment, the constituents absorbed into blood are mainly from Rhei Radix et Rhizoma and Aconiti Lateralis Radix Praeparata, and the serum pharmacochemistry signs could provide the evidence for the further research of the material basis of the decoction.
ABSTRACT
Objective: To study the adscription and identification of transitional constituents in serum of rats after ig administration of petroleum ether fraction in ethanol extract from Xiaoyao Powder (XYP-A). Methods: A UPLC-PDA method was established to identify the active components in serum of rats after ig administration of XYP-A and the single preparations of Bupleuri Radix (BR) Angelicae Sinensis Radix (ASR), and Atractylodis Macrocephalae Rhizoma (AMR). The transitional constituents were analyzed by comparing the fingerprints of the serum samples in formula, the single preparations and reference substances, referring literature, retention time, and UV scan spectra. Results: Twenty components including 12 original components from XYP-A and eight metabolites were detected in serum of rats after ig administration of XYP-A. The original components consisted nine derived from BR, two from ASR, one from AMR, and the structures of ligustilide, atractylenolide II, 2, 8, 10-pentadecatriene-4, 6-diyne-1-ol (CH-1), and bupleurynol (CH-2) were identified. Conclusion: The method is successfully applied for the serum pharmacochemistry study in rats. Twenty transitional constituents are absorbed into serum of rats and their metabolites may be the effective constituents of XYP-A acting directly to the body, which could lay the foundation for the serum pharmacodynamic study.
