Soil Type Influences Novel "Milpa" Isolates of Trichoderma virens and Aspergillus tubingensis That Promote Solubilization, Mineralization, and Phytoabsorption of Phosphorus in Capsicum annuum L.

The Capsicum genus has significant economic importance since it is cultivated and consumed worldwide for its flavor and pungent properties. In 2021, Mexico produced 3.3 billion tons on 45,000 hectares which yielded USD 2 billion in exports to the USA, Canada, Japan, etc. Soil type has a dramatic effect on phosphorus (P) availability for plantsdue to its ion retention.In a previous study, novel fungal isolates were shown to solubilize and mineralize P in different kinds of soils with different P retention capacities. The aim of this work was to study the effects of the mineralogy of different kinds of "milpa" soils on the germination, biomass production, and P absorption of chili plants (Capsicum annuum). The germination percentage, the germination speed index, and the mean germination time were significantly increased in the plants treated with dual inoculation. Foliar phosphorus, growth variables, and plant biomass of chili plants grown in a greenhouse were enhanced in different soil types and with different inocula. Correlation studies suggested that the most significant performance in the foliar P concentration and in the growth response of plants was achieved in Vertisol with dual inoculation of 7 × 106 mL-1 spores per chili plant, suggesting this would be an appropriate approach to enhance chili cultivation depending on the soil type. This study stresses the importance of careful analysis of the effect of the soil type in the plant-microbe interactions.

Using large-scale tropical dry forest restoration to test successional theory.

Microclimatic conditions change dramatically as forests age and impose strong filters on community assembly during succession. Light availability is the most limiting environmental factor in tropical wet forest succession; by contrast, water availability is predicted to strongly influence tropical dry forest (TDF) successional dynamics. While mechanisms underlying TDF successional trajectories are not well understood, observational studies have demonstrated that TDF communities transition from being dominated by species with conservative traits to species with acquisitive traits, the opposite of tropical wet forest. Determining how functional traits predict TDF tree species' responses to changing environmental conditions could elucidate mechanisms underlying tree performance during TDF succession. We implemented a 6-ha restoration experiment on a degraded Vertisol in Costa Rica to determine (1) how TDF tree species with different resource-use strategies performed along a successional gradient and (2) how ecophysiological functional traits correlated with tree performance in simulated successional stages. We used two management treatments to simulate distinct successional stages including: clearing all remnant vegetation (early-succession), or interplanting seedlings with no clearing (mid-succession). We crossed these two management treatments (cleared/interplanted) with two species mixes with different resource-use strategies (acquisitive/conservative) to examine their interaction. Overall seedling survival after 2 yr was low, 15.1-26.4% in the four resource-use-strategy × management-treatment combinations, and did not differ between the management treatments or resource-use-strategy groups. However, seedling growth rates were dramatically higher for all species in the cleared treatment (year 1, 69.1% higher; year 2, 143.3% higher) and defined resource-use strategies had some capacity to explain seedling performance. Overall, ecophysiological traits were better predictors of species' growth and survival than resource-use strategies defined by leaf and stem traits such as specific leaf area. Moreover, ecophysiological traits related to water use had a stronger influence on seedling performance in the cleared, early-successional treatment, indicating that the influence of microclimatic conditions on tree survival and growth shifts predictably during TDF succession. Our findings suggest that ecophysiological traits should be explicitly considered to understand shifts in TDF functional composition during succession and that using these traits to design species mixes could greatly improve TDF restoration outcomes.

Estimación de erosión potencial bajo dos sistemas de corte de caña panelera utilizando la ecuación universal de pérdida de suelos / Estimation of potential erosion under two sugarcane cutting systems using the universal soil loss equation

RESUMEN Con el fin de comparar las pérdidas de suelo en dos sistemas de corte tradicionalmente utilizados en el cultivo de caña de azúcar para la producción de panela, se llevó a cabo un estudio, en el que se realizaron mediciones en campo, utilizando un simulador de lluvia y parcelas de escorrentía, en un vertisol del municipio de Quebradanegra, Cundinamarca, en donde se presentan, simultáneamente, los dos tipos de corte: parejo (tipo de corte en el que se cosecha todo a la vez, al tener una madurez homogénea en el cultivo) y entresaque (sistema en el cual no hay madurez homogénea, por lo que se corta solo un porcentaje de las plantas a la vez). Para estimar las pérdidas de suelo potenciales asociadas a ambos sistemas de corte, se utilizó la Ecuación Universal de Pérdida de Suelos (USLE), obteniendo un factor medio C, asociado al tipo de corte, utilizando los datos determinados en campo y la erosividad de las intensidades de precipitación simuladas. Finalmente, se determinó la erosión potencial anual, bajo los dos sistemas de corte, donde se observó que el sistema de corte por parejo presenta un valor 2,2 veces mayor que el sistema de corte por entresaque. Por otra parte, al comparar las pérdidas potenciales de suelo con un lote recién preparado y sembrado, sin cobertura vegetal, se evidencia la capacidad del cultivo de caña panelera para brindar protección al suelo, presentando una erosión potencial 9 veces menor, en el caso del corte por parejo, con respecto a una condición de suelo sin cobertura vegetal.

ABSTRACT In order to compare soil losses in two cutting systems traditionally used in the cultivation of sugar cane for the production of panela, a study was carried out in which measurements were made in the field using a rainfall simulator and runoff plots in a vertisol of the municipality of Quebradanegra, Cundinamarca, where both types of cut are presented simultaneously: the cut-off system, in which everything is harvested at once having a homogeneous maturity in the crop and the thinning cut system, in which have an inhomogeneous maturity so that only a percentage of the plants is cut at the same time. To estimate the potential soil losses associated with both cutting systems, the Universal Soil Loss Equation (USLE) was used to obtain a mean C factor associated with the type of cut, using the data determined in the field and the erosivity of simulated rainfall intensities. Finally, the anual potential losses of soil was determined under the two cutting systems, where it was observed that the cut-off system has an annual potential erosion 2.2 times greater than the thinning cuy system. On the other hand, when comparing the potential losses of soil with a newly prepared and planted lot, without plant cover, the capacity of the sugarcane cane crop is demonstrated to offer a protection to the soil, presenting a potential erosion 9 times smaller, in the case of the cut-off system, with respect to a soil condition without plant cover.

Fertirrigación con diferentes formas de nitrógeno en el cultivo de tomate en un suelo arcilloso / Tomato crop fertigation with different nitrogen form in clayed soil / Fertirrigação com diferentes formas de nitrogênio no cultivo de tomate em um solo argiloso

Se estudió el efecto de nitrógeno nítrico, amoniacal y ureico, suministrados en distintas etapas de desarrollo del cultivo de tomate, sobre el rendimiento y la calidad de fruto. El trabajo se realizó en campo bajo riego por goteo, en un suelo vertisol pélico y clima cálido y semiárido. Tres tratamientos de fertilización (T1, T2 y testigo) fueron distribuidos en un diseño de bloques completos al azar con tres repeticiones. T1 y T2 se fertilizaron con 250-50-190kg·ha-1 de N, P y K, respectivamente; en estos el N se suministró en diferentes proporciones de N-ureico, N-amoniacal y N-nítrico, en distintas etapas de desarrollo del cultivo; el T3 se aplicó como los productores del Valle de Culiacán, Sinaloa, México (450-118-413kg·ha-1 de N-P-K), con 75% del N como nitrato. La concentración de N-NO3 en el extracto celular de peciolos en los T1 y T2 fluctuó de 500 a 1360ppm, y en T3 fue de 300-1175ppm. Estos valores estuvieron relacionados con la dosis de N total suministrado pero no con la proporción de NH4/NO3 aplicada, y tampoco influyeron en la producción de fruto de exportación. La producción de fruto fue estadísticamente igual en las dosis de fertilización alta y moderada, con un ahorro del 75% de N-nítrico. En calidad poscosecha de frutos, la fertilización reducida y la aplicación de N-ureico y N-amoniacal en altas proporciones no afectó la firmeza, ºBrix ni la pérdida de peso de los frutos.

The effect of nitrogen form (urea, nitrate and ammonium) provided at various stages of development of the tomato crop on the yield and quality of fruit was studied. The work was conducted under field conditions in a pellustert soil, drip irrigation and a warm and semi-arid climate. Three fertilization treatments (T1, T2 and control) were arranged in a randomized complete-block design with three replicates. T1 and T2 were fertilized with 250-50-190kg·ha-1 N, P and K, respectively; which N was supplied in different ratios of ureic-N, ammonium-N and nitric-N along growing tomato plants; T3 was provided in a similar way as is generally employed by the tomato producers in the Culiacan Valley, Sinaloa, Mexico (450-118-413kg·ha-1 N-P-K), with 75% of N in nitrate form. The concentration of N-NO-3 in the petiole cell extract in T1 and T2 ranged from 500 to 1360ppm, and in the control it was 300-1175ppm. These values were related with the total N doses supplied but not with the ratio of NH4/NO3 provided, neither influenced on the total amount of exportation fruit. The fruit production was statistically similar in the high and moderated fertilizer doses with a fertilizer savings of 75% of nitrate N. With respect to the post-harvest quality in the tomato fruit, moderated fertilization associated with high application of ureic-N and ammonium-N did not affect the fruit firmness, ºBrix or the weight loss in the fruit.

Estudou-se o efeito do nitrogênio nítrico, amoniacal e uréico, subministrados em distintas etapas de desenvolvimento do cultivo de tomate, sobre o rendimento e a qualidade do fruto. O trabalho se realizou no campo sob irrigação por gotejamento, em um solo vertisol pélico e clima cálido e semi-árido. Três tratamentos de fertilização (T1, T2 e testemunho) foram distribuídos em um desenho de blocos completos aleatório com três repetições. T1 e T2 se fertilizaram com 250-50-190kg•ha-1 de N, P e K, respectivamente; em estes o N se subministrou em diferentes proporções de N-uréico, N-amoniacal e N-nítrico, em distintas etapas de desenvolvimento do cultivo; o T3 se aplicou como os produtores do Vale de Culiacán, Sinaloa, México (450-118-413kg•ha-1 de N-P-K), com 75% do N como nitrato. A concentração de N-NO3 no extrato celular de pecíolos nos T1 e T2 flutuou de 500 a 1360ppm, e em T3 foi de 300-1175ppm. Estes valores estiveram relacionados com a dose de N total subministrado, mas não com a proporção de NH4/NO3 aplicada, e tampouco influíram na produção do fruto de exportação. A produção do fruto foi estatisticamente igual nas doses de fertilização alta e moderada, poupando 75% de N-nítrico. Em qualidade pos-colheita de frutos, a fertilização reduzida e a aplicação de N-uréico e N-amoniacal em altas proporções não afetou a firmeza, oBrix nem a perda de peso dos frutos.