Prediction of Nitrogen Dosage in 'Alicante Bouschet' Vineyards with Machine Learning Models.

Vineyard soils normally do not provide the amount of nitrogen (N) necessary for red wine production. Traditionally, the N concentration in leaves guides the N fertilization of vineyards to reach high grape yields and chemical composition under the ceteris paribus assumption. Moreover, the carryover effects of nutrients and carbohydrates stored by perennials such as grapevines are neglected. Where a well-documented database is assembled, machine learning (ML) methods can account for key site-specific features and carryover effects, impacting the performance of grapevines. The aim of this study was to predict, using ML tools, N management from local features to reach high berry yield and quality in 'Alicante Bouschet' vineyards. The 5-year (2015-2019) fertilizer trial comprised six N doses (0-20-40-60-80-100 kg N ha-1) and three regimes of irrigation. Model features included N dosage, climatic indices, foliar N application, and stem diameter of the preceding season, all of which were indices of the carryover effects. Accuracy of ML models was the highest with a yield cutoff of 14 t ha-1 and a total anthocyanin content (TAC) of 3900 mg L-1. Regression models were more accurate for total soluble solids (TSS), total titratable acidity (TTA), pH, TAC, and total phenolic content (TPC) in the marketable grape yield. The tissue N ranges differed between high marketable yield and TAC, indicating a trade-off about 24 g N kg-1 in the diagnostic leaf. The N dosage predicted varied from 0 to 40 kg N ha-1 depending on target variable, this was calculated from local features and carryover effects but excluded climatic indices. The dataset can increase in size and diversity with the collaboration of growers, which can help to cross over the numerous combinations of features found in vineyards. This research contributes to the rational use of N fertilizers, but with the guarantee that obtaining high productivity must be with adequate composition.

Assessment of the vineyard water footprint by using ancillary data and EEFlux satellite images. Examples in the Chilean central zone.

The increase of vineyard's water consumption due to the Global Warming Phenomenon (GWP) has forced the winegrowers to strengthen their irrigation and water stewardship efforts, intended for maintaining this resource's long-term sustainable use. Due to water being a limited resource, implementing the Water Footprint (WF) concept in winegrapes production provides helpful information for sustainable water stewardship. Currently, an automated version of the satellite-based METRIC (Mapping Evapotranspiration with Internalized Calibration) model, the Google Earth Engine Evapotranspiration Flux (EEFlux) platform, has been suggested as an alternative to analyzing the spatial variability of an entire field's water consumption throughout the growing season. This work aimed to evaluate the potential application of the EEFlux satellite's actual evapotranspiration (ETa) products and ancillary field data to obtain the WF blue (WFb) and green (WFg) of six commercial vineyards placed in the Chilean central zone. Firstly, the reliability of the daily actual evapotranspiration data from EEFlux (ETa EEFlux) was assessed against measured ETa data, using an available database from previous studies. The results of ETa EEFlux estimations against measured ETa were impressive, presenting a root square error (RMSE) of 0.8 mm day-1. The satellite-derived crop coefficients (Kc Sat) allowed to estimate the total WF of each vineyard, in a range of 200 to 900 m3 t-1, showing an average relative error (RE) of 101%, between the satellite-based WFb (WFb Sat) and those calculated from irrigation records (WFb). These results reflected the particular conditions of each vineyard and can be considered reasonable since they were estimated from ancillary data and EEFlux products. This study provides new insights that may represent opportunities to sustainably managing the irrigation of vineyards.

Influence of Climate on Soil and Wine Bacterial Diversity on a Vineyard in a Non-traditional Wine Region in Argentina.

Argentina is the fifth world-wide wine producer, with an area of emerging importance in the Southwest of Buenos Aires Province, where climatic conditions are rather challenging. We studied the variations in soil and wine bacterial diversity through three consecutive vintages, and how climatic conditions affected said diversity. During the years of our study there were two harsh climatic events, a prolonged drought that extended over two vegetative periods, and an unseasonable spring frost in 2017. We found that the bacterial diversity reacted to these climatic events, given that there was a shift in the taxa exclusive to soil and wine, and shared by both, through time. Our results show a core of microorganisms in soil as well as in wine, belonging to different phyla that are conserved across the vintage years. A trend to an enrichment in Actinobacteria was detected in soil samples, whereas a high relative abundance of the Acetobacteraceae family and a scarcity of Lactic Acid Bacteria (LAB) were detected in the wine samples. We believe our results contribute to a better understanding of the impact of climatic conditions on the soil and wine microbiota, and can provide vintners with valuable knowledge for improving their wine production.

Fungal diversity and occurrence of mycotoxin producing fungi in tropical vineyards.

Grapevine cultivars are distributed worldwide, nevertheless the fermentation of its grape berries renders distinct wine products that are highly associated to the local fungal community. Despite the symbiotic association between wine and the fungal metabolism, impacting both the terroir and mycotoxin production, few studies have explored the vineyard ecosystem fungal community using both molecular marker sequencing and mycotoxin production assessment. In this study, we investigated the fungal community of three grapevine cultivars (Vitis vinifera L.) in two tropical vineyards. Illumina MiSeq sequencing was performed on two biocompartments: grape berries (GB) and grapevine soil (GS); yielding a total of 578,495 fungal internal transcribed spacer 1 reads, which were used for taxonomic classification. GB and GS fungal communities were mainly constituted by Ascomycota phylum. GS harbors a significant richer and more diverse fungal community than GB. Among GB samples, Syrah grape berries exclusively shared fungal community included wine-associated yeasts (e.g. Saccharomycopsis vini) that may play key roles in wine terroir. Mycotoxin production assessment revealed the high potential of Aspergillus section Flavi and Penicillium section Citrina isolates to produce aflatoxin B1-B2 and citrinin, respectively. This is the first study to employ next-generation sequencing to investigate vineyard associated fungal community in Brazil. Our findings provide valuable insights on the available tools for fungal ecology assessment applied to food products emphasizing the coexistence between classical and molecular tools.

Soil tillage affects soybean growth and promotes heavy metal accumulation in seeds.

When soybean is grown in soils with high heavy metal concentrations, it may introduce those contaminants into the human food chain, posing risks to human health. This study evaluated the effect of tilling the soil with high Cu, Zn, and Mn levels on soybean physiology and metal accumulation in seeds. Disturbed and undisturbed soil samples were collected in two different sites: a vineyard with high heavy metal concentration and a grassland area, containing natural vegetation. Two soybean cultivars were sown and grown in the greenhouse. Photosynthetic parameters and biochemical analysis of oxidative stress were performed. Cu, Zn, and Mn in leaves and seeds, dry mass, and weight of seeds were evaluated. Soil structure had a high influence on plant growth and physiology, while soil site had a high impact on heavy metal accumulation in leaves and seeds. Soybean plants that grown in vineyard soils with high heavy metal concentrations, accumulated 50% more Zn in leaves and seeds, 70% more Cu in leaves, and 90% more Cu in seeds, than those plants grown in grassland soils. Besides, Zn concentration in seeds was higher than the permissible limit. Moreover, the disturbance of both vineyard soil and grassland soil was not good for plant growth and physiology, which have increased TBARS and H2O2 concentration in plants, transpiration rate, metal concentration in leaves and seeds. Soil disturbance may have caused organic matter oxidation and changes in the composition and quantity of soil microorganisms and it affects the availability of other nutrients in the soil.

Water and suspended sediment runoff from vineyard watersheds affecting the behavior and physiology of zebrafish.

Viticulture plays an important role in generating income for small farms globally. Historically, vineyards use large quantities of phytosanitary products, such as Bordeaux mixture [Ca(OH)2 + CuSO4], to control plant diseases. These products result in the accumulation of copper (Cu) in the soil and increases the risk of transfer to water bodies. Thus, it is important to evaluate whether the presence of Cu-bearing particles in water is toxic to aquatic fauna. This study conducted chemical, mineralogical, and particle size evaluations on water samples and sediments collected from a watershed predominantly cultivated with old vineyards. The proportion of Cu-rich nanoparticles (<10 nm) in the sediment was ~27%. We exposed zebrafish to different dilutions of water and sediment samples that collected directly from the study site (downstream river) under laboratory conditions. Then, we evaluated their exploratory behavior and the stress-related endocrine parameter, whole-body cortisol. We also carried out two experiments in which zebrafish were exposed to Cu. First, we determined the median lethal concentration (LC50-96 h) of Cu and then assessed whether Cu exposure results in effects similar to those associated with exposure to the water and sediment samples collected from the study site. The water and sediment samples directly impacted the exploratory behavior of zebrafish, showing clear anxiety-like behavioral phenotype and stress in terms of cortisol increase (during the second rain event). The Cu exposure did not mimic the same behavioral changes triggered by the water and sediment samples, although it had caused similar stress in the fish. Our results highlight that even at low concentrations, the water and sediment samples from vineyard watershed runoff were able to induce behavioral and endocrine changes that may harm the ecological balance of an aquatic environment.

Growing Industries, Growing Invasions? The Case of the Argentine Ant in Vineyards of Northern Argentina.

The invasive Argentine ant causes ecological and economic damage worldwide. In 2011, this species was reported in vineyards of Cafayate, a wine-producing town in the Andes, Argentina. While the local xeric climate is unsuitable for Argentine ants, populations could establish in association with vineyards where human activity and irrigation facilitate propagule introduction and survival. In 2013-2014, we combined extensive sampling of the area using ant-baits with monitoring of the change in land use and vineyard cultivated area over the past 15 years. Our results revealed that the species has thus far remained confined to a relatively isolated small area, owing to an effective barrier of dry shrublands surrounding the infested vineyards; yet the recent expansion of vineyard acreage in this region will soon connect this encapsulated area with the rest of the valley. When this happens, vulnerable ecosystems and the main local industry will be put at risk. This case provides a rare opportunity to study early invasion dynamics and reports, to the best of our knowledge, for the first time, the Argentine ant in high altitude agroecosystems.

Evaluation of a decision support strategy for the control of powdery mildew, Erysiphe necator (Schw.) Burr., in grapevine in the central region of Chile.

BACKGROUND: The primary strategy to control powdery mildew in Chilean vineyards involves periodic fungicide spraying, which may lead to many environmental and human health risks. This study aimed to implement and evaluate the effectiveness and economic feasibility of a novel decision support strategy (DSS) to limit the number of treatments against this pathogen. An experiment was conducted between the 2010 and 2013 seasons in two irrigated vine fields, one containing a cultivar of Cabernet Sauvignon (CS) and the other a cultivar of Chardonnay (CH). RESULTS: The results showed that the DSS effectively controlled powdery mildew in CS and CH vine fields, as evidenced by a disease severity lower than 3%, which was lower than that observed in untreated vines (approximately 10 and 40% for CS and CH respectively). The DS strategy required the application of only 2-3 fungicide treatments per season in key vine phenological stages, and the cost fluctuated between $US 322 and 415 ha-1 , which was 40-60% cheaper than the traditional strategy employed by vine growers. CONCLUSION: The decision support strategy evaluated in this trial allows a good control of powdery mildew for various types of epidemic with an early and late initiation. © 2017 Society of Chemical Industry.

Impact of a pesticide cocktail (fenhexamid, folpel, deltamethrin) on the abundance of Glomeromycota in two agricultural soils.

Pesticide contamination of the environment can result from agricultural practices. Persistence of pesticide residues is a threat to the soil biota including plant roots and beneficial microorganisms, which support an important number of soil ecosystem services. Arbuscular mycorrhizal fungi (AMF) are key symbiotic microorganisms contributing to plant nutrition. In the present study, we assessed whether AMF could indicate eventual side effects of pesticides when directly applied to field soils. We evaluated the ecotoxicological impact of a cocktail of three commonly used agricultural pesticides (fenhexamid, folpel, deltamethrin) on the abundance and composition of the AMF community in vineyard (Montagne de Saint-Emilion) and arable (Martincourt) soils subjected to different agricultural practices. The dissipation of applied pesticides was monitored by multiresidual analyses to determine the scenario of exposure of the AMF community. Diversity analysis before application of the pesticide cocktail showed that the AMF communities of vineyard soils, subjected to mechanical weeding or grass cover, and of the arable soil subjected to intensive agriculture, were dominated by Glomerales. Ribotypes specific to each soil and to each agricultural practice in the same soil were found, with the highest abundance and diversity of AMF being observed in the vineyard soil with a grass-cover. The abundance of the global AMF community (Glomeromycota) and of three taxa of AMF (Funneliformis mosseae, Claroideoglomus etunicatum/C. claroideum) was evaluated after pesticide application. The abundance of Glomeromycota decreased in both soils after pesticide application while the abundance of Claroideoglomus and F. mosseae decreased only in the arable soil. These results show that higher doses of pesticide exposure did not affect the global abundance, but altered the composition, of the AMF community. Resilience of the AMF community composition was observed only in the vineyard soil, where F. mosseae was the most tolerant taxon to pesticide exposure.

Copper accumulation in vineyard soils: Rhizosphere processes and agronomic practices to limit its toxicity.

Viticulture represents an important agricultural practice in many countries worldwide. Yet, the continuous use of fungicides has caused copper (Cu) accumulation in soils, which represent a major environmental and toxicological concern. Despite being an important micronutrient, Cu can be a potential toxicant at high concentrations since it may cause morphological, anatomical and physiological changes in plants, decreasing both food productivity and quality. Rhizosphere processes can, however, actively control the uptake and translocation of Cu in plants. In particular, root exudates affecting the chemical, physical and biological characteristics of the rhizosphere, might reduce the availability of Cu in the soil and hence its absorption. In addition, this review will aim at discussing the advantages and disadvantages of agronomic practices, such as liming, the use of pesticides, the application of organic matter, biochar and coal fly ashes, the inoculation with bacteria and/or mycorrhizal fungi and the intercropping, in alleviating Cu toxicity symptoms.

Agronomic and edaphoclimatic characterization high altitude vineyards / Caracterização agronômica e edafoclimática dos vinhedos de elevada altitude

The objective of this research was to perform the agronomic and edaphoclimatic characterization of vineyards and to determine cultivation trends for the main varieties employed in the production of high altitude fine wines in Santa Catarina State, Brazil. High altitude vineyards were considered those vineyards located 900 m above sea level and vineyards belonging to members of the Santa Catarina Association of Altitude Fine Wines located below 900 m altitude. To assess cultivation trends, the vineyards were geo-referenced, following the Embrapa Uva e Vinho guidelines. To perform the edaphoclimatic characterization, the vineyard polygons were overlaid with physiographic, climatic and soil maps in a Geographic Information System. The characterization of the high altitude vineyards was based on planted area. The varieties with greatest areas were Cabernet Sauvignon (37.9%), Merlot (14.5%) Chardonnay (8.0%) and Sauvignon Blanc (7.5%). From 2009 to 2013 vineyards with C. Sauvignon were replaced for white varieties and for Pinot Noir, especially in altitudes over 1,100 m. A trend in the use of white varieties and Pinot Noir in new vineyards was identified, but the main trend was the increase of experimental areas, with additional 60 varieties of red and white grapes. In relation to physiography, the norm of the Acavitis requires that the vineyards of their associates be located above [...](AU)

O objetivo desse trabalho foi realizar a caracterização agronômica e edafoclimática dos vinhedos e determinar as tendências de uso das principais variedades para produzir vinhos finos nas regiões de elevada altitude do Estado de Santa Catarina. Foram considerados vinhedos de elevada altitude os vinhedos localizados acima de 900 m em relação ao nível do mar e os vinhedos pertencentes aos associados da Associação Catarinense de Vinhos Finos de Altitude (Acavitis) localizados em altitudes inferiores a 900 m. Para avaliar as tendências de cultivo foi realizado um cadastro georreferenciado, seguindo as normas da Embrapa Uva e Vinho. Para realizar a caracterização edafoclimática, as áreas dos vinhedos foram cruzadas com mapas fisiográficos, climáticos e pedológicos em um Sistema de Informações Geográficas. A caracterização dos vinhedos de elevada altitude foi realizada com base na área plantada. As variedades com maior área foram Cabernet Sauvignon (37,9%), Merlot (14,5%), Chardonnay (8,0%) e Sauvignon Blanc (7,5%). De 2009 a 2013 houve substituição de C. Sauvignon por variedades brancas e Pinot Noir, principalmente nas altitudes superiores a 1.100 m. Houve uma tendência no uso de variedades brancas e Pinot Noir em vinhedos novos, mas a principal tendência observada foi no crescimento de áreas experimentais, com outras 60 variedades tintas e brancas. Em relação à fisiografia, a [...](AU)

Agronomic and edaphoclimatic characterization high altitude vineyards / Caracterização agronômica e edafoclimática dos vinhedos de elevada altitude

The objective of this research was to perform the agronomic and edaphoclimatic characterization of vineyards and to determine cultivation trends for the main varieties employed in the production of high altitude fine wines in Santa Catarina State, Brazil. High altitude vineyards were considered those vineyards located 900 m above sea level and vineyards belonging to members of the Santa Catarina Association of Altitude Fine Wines located below 900 m altitude. To assess cultivation trends, the vineyards were geo-referenced, following the Embrapa Uva e Vinho guidelines. To perform the edaphoclimatic characterization, the vineyard polygons were overlaid with physiographic, climatic and soil maps in a Geographic Information System. The characterization of the high altitude vineyards was based on planted area. The varieties with greatest areas were Cabernet Sauvignon (37.9%), Merlot (14.5%) Chardonnay (8.0%) and Sauvignon Blanc (7.5%). From 2009 to 2013 vineyards with C. Sauvignon were replaced for white varieties and for Pinot Noir, especially in altitudes over 1,100 m. A trend in the use of white varieties and Pinot Noir in new vineyards was identified, but the main trend was the increase of experimental areas, with additional 60 varieties of red and white grapes. In relation to physiography, the norm of the Acavitis requires that the vineyards of their associates be located above [...]

O objetivo desse trabalho foi realizar a caracterização agronômica e edafoclimática dos vinhedos e determinar as tendências de uso das principais variedades para produzir vinhos finos nas regiões de elevada altitude do Estado de Santa Catarina. Foram considerados vinhedos de elevada altitude os vinhedos localizados acima de 900 m em relação ao nível do mar e os vinhedos pertencentes aos associados da Associação Catarinense de Vinhos Finos de Altitude (Acavitis) localizados em altitudes inferiores a 900 m. Para avaliar as tendências de cultivo foi realizado um cadastro georreferenciado, seguindo as normas da Embrapa Uva e Vinho. Para realizar a caracterização edafoclimática, as áreas dos vinhedos foram cruzadas com mapas fisiográficos, climáticos e pedológicos em um Sistema de Informações Geográficas. A caracterização dos vinhedos de elevada altitude foi realizada com base na área plantada. As variedades com maior área foram Cabernet Sauvignon (37,9%), Merlot (14,5%), Chardonnay (8,0%) e Sauvignon Blanc (7,5%). De 2009 a 2013 houve substituição de C. Sauvignon por variedades brancas e Pinot Noir, principalmente nas altitudes superiores a 1.100 m. Houve uma tendência no uso de variedades brancas e Pinot Noir em vinhedos novos, mas a principal tendência observada foi no crescimento de áreas experimentais, com outras 60 variedades tintas e brancas. Em relação à fisiografia, a [...]

Factors affecting distribution and abundance of Aspergillus section Nigri in vineyard soils from grapevine growing regions of Argentina.

BACKGROUND: Aspergillus species belonging to section Nigri are the main fungi responsible for ochratoxin (OTA) contamination in grapes and wine. These species live as saprophytes in the superficial layer of the vineyard soil. We evaluated the biodiversity of potentially ochratoxigenic strains of Aspergillus section Nigri isolated from vineyard soils from different grapevine growing regions of Argentina. The isolates were characterized by classical and molecular methods. A multiple correspondence analysis was performed to identify the overall correlation of the Aspergillus group distribution with environmental conditions, geographical characteristics and vineyard practices. RESULTS: Aspergillus niger aggregate was the prevalent group (71%) and A. carbonarius made up only 2%. Species discrimination by species-specific primers showed that in A. niger aggregate 89% were A. tubingensis; 97% of the uniseriate were A. japonicus/A. aculeatus. Isolates belonging to these groups were unable to produce OTA. Our results clearly demonstrate a strong association between presence of A. carbonarius, high average temperatures and drip irrigation. Precipitation levels appear as a secondary factor, and altitude, vineyard age, predominant species, grape variety or total fungal count showed no association with A. carbonarius. CONCLUSION: We demonstrated a low prevalence of ochratoxigenic species in vineyard soil from the grape-growing regions of Argentina.

Evaluation of copper resistant bacteria from vineyard soils and mining waste for copper biosorption

Vineyard soils are frequently polluted with high concentrations of copper due application of copper sulfate in order to control fungal diseases. Bioremediation is an efficient process for the treatment of contaminated sites. Efficient copper sorption bacteria can be used for bioremoval of copper from contaminated sites. In this study, a total of 106 copper resistant bacteria were examined for resistance to copper toxicity and biosorption of copper. Eighty isolates (45 from vineyard Mollisol, 35 from Inceptisol) were obtained from EMBRAPA (Empresa Brasileira de Pesquisa Agropecuária) experimental station, Bento Gonçalves, RS, Brazil (29º09'53.92''S and 51º31'39.40''W) and 26 were obtained from copper mining waste from Caçapava do Sul, RS, Brazil (30º29'43.48''S and 53'32'37.87W). Based on resistance to copper toxicity and biosorption, 15 isolates were identified by 16S rRNA gene sequencing. Maximal copper resistance and biosorption at high copper concentration were observed with isolate N2 which removed 80 mg L-1 in 24 h. Contrarily isolate N11 (Bacillus pumilus) displayed the highest specific copper biosorption (121.82 mg/L/OD unit in 24 h). GenBank MEGABLAST analysis revealed that isolate N2 is 99 percent similar to Staphylococcus pasteuri. Results indicate that several of our isolates have potential use for bioremediation treatment of vineyards soils and mining waste contaminated with high copper concentration.

Evaluation of copper resistant bacteria from vineyard soils and mining waste for copper biosorption.

Vineyard soils are frequently polluted with high concentrations of copper due application of copper sulfate in order to control fungal diseases. Bioremediation is an efficient process for the treatment of contaminated sites. Efficient copper sorption bacteria can be used for bioremoval of copper from contaminated sites. In this study, a total of 106 copper resistant bacteria were examined for resistance to copper toxicity and biosorption of copper. Eighty isolates (45 from vineyard Mollisol, 35 from Inceptisol) were obtained from EMBRAPA (Empresa Brasileira de Pesquisa Agropecuária) experimental station, Bento Gonçalves, RS, Brazil (29°09'53.92″S and 51°31'39.40″W) and 26 were obtained from copper mining waste from Caçapava do Sul, RS, Brazil (30°29'43.48″S and 53'32'37.87W). Based on resistance to copper toxicity and biosorption, 15 isolates were identified by 16S rRNA gene sequencing. Maximal copper resistance and biosorption at high copper concentration were observed with isolate N2 which removed 80 mg L(-1) in 24 h. Contrarily isolate N11 (Bacillus pumilus) displayed the highest specific copper biosorption (121.82 mg/L/OD unit in 24 h). GenBank MEGABLAST analysis revealed that isolate N2 is 99% similar to Staphylococcus pasteuri. Results indicate that several of our isolates have potential use for bioremediation treatment of vineyards soils and mining waste contaminated with high copper concentration.

Evaluation of copper resistant bacteria from vineyard soils and mining waste for copper biosorption

Vineyard soils are frequently polluted with high concentrations of copper due application of copper sulfate in order to control fungal diseases. Bioremediation is an efficient process for the treatment of contaminated sites. Efficient copper sorption bacteria can be used for bioremoval of copper from contaminated sites. In this study, a total of 106 copper resistant bacteria were examined for resistance to copper toxicity and biosorption of copper. Eighty isolates (45 from vineyard Mollisol, 35 from Inceptisol) were obtained from EMBRAPA (Empresa Brasileira de Pesquisa Agropecuária) experimental station, Bento Gonçalves, RS, Brazil (29º09'53.92''S and 51º31'39.40''W) and 26 were obtained from copper mining waste from Caçapava do Sul, RS, Brazil (30º29'43.48''S and 53'32'37.87W). Based on resistance to copper toxicity and biosorption, 15 isolates were identified by 16S rRNA gene sequencing. Maximal copper resistance and biosorption at high copper concentration were observed with isolate N2 which removed 80 mg L-1 in 24 h. Contrarily isolate N11 (Bacillus pumilus) displayed the highest specific copper biosorption (121.82 mg/L/OD unit in 24 h). GenBank MEGABLAST analysis revealed that isolate N2 is 99% similar to Staphylococcus pasteuri. Results indicate that several of our isolates have potential use for bioremediation treatment of vineyards soils and mining waste contaminated with high copper concentration.

Modelo de predicción para Botrytis cinerea en un viñedo de Galicia (España) / Predictive model for Botrytis cinerea in a vineyard of Galicia, Spain

Se realizó un recuento de las esporas de Botrytis cinerea en la atmósfera de un viñedo de la comarca del Ribeiro (Galicia, España), durante tres años consecutivos. Para explicar su abundancia, dicha información se ha relacionado con las fases fenológicas de la vid y con la influencia que los principales parámetros meteorológicos ejercen sobre el contenido fúngico. Tras el estudio estadístico de los datos se ha elaborado un modelo predictivo (regresión polinomial) de la concentración de dichas esporas en la atmósfera.

During three consecutive years, the counting of Botrytis cinerea spores in the atmosphere of a vineyard in the region of Ribeiro (Galicia, Spain) has been carried out. To explain their abundance, such information has been related to the phonological stages of the vine and to the influence that the main meteorological parameters exert on the fungal content. Taking into account the statistical analysis of data, a predictive model (polynomial regression) for the concentration of these spores in the atmosphere has been prepared.

Copper concentration of vineyard soils as a function of pH variation and addition of poultry litter

Copper (Cu) concentration was evaluated as a function of pH variation and addition of poultry litter to a Dystrophic Lithic Udorthent and a Humic Dystrudept from the state of Rio Grande do Sul, Brazil, cultivated with vines treated with successive applications of Cu-based product. Samples were collected from the surface layer (0 to 10 cm). Soluble Cu concentration was determined using DTPA and Mehlich III as extractants, and exchangeable Cu was determined in CaCl2. The availability of Cu was mainly affected by the soil pH. CaCl2 extractant had the best correlation with Cu concentration in contaminated soils, according to treatments applied. The addition of poultry litter did not reduce Cu availability in these soils. Total soil Cu content varied between 1,300 and 1,400 mg kg-1 in both soils. Copper available fractions, extracted by DTPA, CaCl2 and Mehlich III, averaged 35, 0.2 and 63 percent, respectively, of the total Cu present in the soil.

Avaliaram-se os teores de Cu em função da variação do pH e da adição de cama-de-frango de dois solos com elevados teores deste elemento. Foram coletadas amostras da camada superficial (0 a 10 cm) de um typical dystrophic Lithic Udorthent - LU (Neossolo Litólico distrófico típico) e de um Humic Dystrudept - HD (Cambissolo Húmico alumínico típico) da região da Serra do RS, cultivados com parreirais que receberam aplicações sucessivas de produtos à base de Cu. Foram determinados os teores de Cu solúvel em DTPA e pelo método Mehlich III, além do Cu trocável em CaCl2. A disponibilidade de Cu foi afetada principalmente pelo pH do solo. O extrator CaCl2 foi o que melhor se correlacionou com os teores de Cu em solos contaminados em função dos tratamentos aplicados. A adição de cama-de-frango não diminuiu a disponibilidade de Cu destes solos. Os teores de Cu total variaram entre 1.300 e 1.400 mg kg-1 nos dois solos. Considerando os teores totais de Cu nos solos, as frações "disponíveis", extraídas por DTPA, por CaCl2 e por Mehlich III representaram, em média, 35 por cento, 0,20 por cento e 63 por cento do total de Cu presente no solo, respectivamente.

Ocorrência de Cryptoblabes gnidiella (Millière) (Lepidoptera: Pyralidae) relacionada à fenologia da videira em Bento Gonçalves, RS / Ocurrence of Cryptoblabes gnidiella (Millière) (Lepidoptera: Pyralidae) associated with grape phenology in Bento Gonçalves, RS

O objetivo deste estudo foi registrar e comparar a ocorrência de adultos e imaturos da traça-dos-cachos, Cryptoblabes gnidiella (Millière), em dois vinhedos, nos diferentes estágios fenológicos de Vitis vinifera cv. Pinot Noir, em Bento Gonçalves (29°10'S 51°32'O), RS, no período de 2004-2005. Quinzenalmente a população de imaturos foi monitorada em cachos e ramos de videira, enquanto os adultos, em armadilhas com feromônio sexual sintético. Houve diferença significativa entre o número de indivíduos coletados nos diferentes estágios fenológicos da videira. No período de cachos secos registrou-se o maior número de C. gnidiella. A permanência de cachos secos nos vinhedos após a colheita permite o refúgio dos imaturos de C. gnidiella no período de entressafra.

The aim of this study was to record and compare the occurrence of adults and immature stages of honeydew moth, Cryptoblabes gnidiella (Millière), related with grape phenology in two Pinot Noir (Vitis vinifera) vineyards in Bento Gonçalves (29°10'S 51°32'O), RS, during 2004-2005. Fortnightly, immature stages were monitored in the clusters and grapevine branches, while the adults, in delta traps baited with synthetic sex pheromone. There were significant differences in the number of individuals in different phenologic stages of vineyards. The highest catches of honeydew moth were registered in the period of dry clusters. The permanence of dry cluster after harvest, could serve as refuge for C. gnidiella immatures between seasons.

Efeito da fertilização nitrogenada em videira sobre a formação de alguns compostos volatéis no vinho

Vitis vinifera Gewürztraminer (white variety) and Cabemet Sauvignon (red) growned in Santana do Livramento, RS, and Garibaldi, RS, were added with different nitrogen treatments in the vineyard during me 1992-1993 season. After harvest and crushing, the musts were divided into four lots each one added with a different yeast. The volatiles were analyzed through GC. Maximum amount of methanol was found with the red variety (225.6mg/l). Increasing nitrogen in the soil increased (mg/l) propanol-1 (min. 25-max. 78.2) and decreased methyl-2 propanol-1 (min. 60.8-max. 125.9) and methyl-3+methyl-2 butanol-1 (min. 85.8-max. 407.8). The fusel alcohol fraction showed, also, differences between region,grape variety and yeast used.

Vitis vinifera cv. Gewürztraminer e Cabemet Sauvignon cultivadas em Santana do Livramento e Garibaldi foram submetidas a diferentes tratamentos nitrogenados realizados durante o período vegetativo de 1992-1993. Após a colheita e esmagamento o mosto obtido foi dividido em quatro lotes e fermentado com diferentes leveduras. As determinações dos compostos voláteis foram realizados através de cromatografia gasosa. Maiores valores de metanol foram encontrados nos vinhos de Cabemet Sauvignon (máximo 225,6mg/l). A fertilização nitrogenada provocou um aumento na concentração (mg/l) de propanol-1 (min. 25 -máx. 78,2) e uma diminuição de metil-2 propanol-1 (min. 60,8 - máx. 125,9) e metil-3 + metil-2 butanol-1 (min. 85,8 - máx. 407,8) nos vinhos. Os álcoois superiores apresentaram também diferenças em relação à região, cultivar e levedura utilizada.