Measuring the viscoelastic relaxation function of cells with a time-dependent interpretation of the Hertz-Sneddon indentation model.

The Hertz-Sneddon elastic indentation model is widely adopted in the biomechanical investigation of living cells and other soft materials using atomic force microscopy despite the explicit viscoelastic nature of these materials. In this work, we demonstrate that an exact analytical viscoelastic force model for power-law materials, can be interpreted as a time-dependent Hertz-Sneddon-like model. Characterizing fibroblasts (L929) and osteoblasts (OFCOLII) demonstrates the model's accuracy. Our results show that the difference between Young's modulus EY obtained by fitting force curves with the Hertz-Sneddon model and the effective Young's modulus derived from the viscoelastic force model is less than 3%, even when cells are probed at large forces where nonlinear deformation effects become significant. We also propose a measurement protocol that involves probing samples at different indentation speeds and forces, enabling the construction of the average viscoelastic relaxation function of samples by conveniently fitting the force curves with the Hertz-Sneddon model.

Unveiling the role of hydroxyapatite and hydroxyapatite/silver composite in osteoblast-like cell mineralization: An exploration through their viscoelastic properties.

Mechanical properties are becoming fundamental for advancing the comprehension of cellular processes. This study addresses the relationship between viscoelastic properties and the cellular mineralization process. Osteoblast-like cells treated with an osteogenic medium were employed for this purpose. Additionally, the study explores the impact of hydroxyapatite (HA) and hydroxyapatite/silver (HA/Ag) composite on this process. AFM relaxation experiments were conducted to extract viscoelastic parameters using the Fractional Zener (FZ) and Fractional Kelvin (FK) models. Our findings revealed that the main phases of mineralization are associated with alterations in the viscoelastic properties of osteoblast-like cells. Furthermore, HA and HA/Ag treatments significantly influenced changes in the viscoelastic properties of these cells. In particular, the HA/Ag treatment demonstrated a marked enhancement in cell fluidity, suggesting a possible role of silver in accelerating the mineralization process. Moreover, the study underscores the independence observed between fluidity and stiffness, indicating that modifications in one parameter may not necessarily correspond to changes in the other. These findings shed light on the factors involved in the cellular mineralization process and emphasize the importance of using viscoelastic properties to discern the impact of treatments on cells.

On the contribution of solid and fluid behavior to the modeling of the time-dependent mechanics of tendons under semi-confined compression.

The present work aims to investigate whether it is possible to identify and quantify the contributions of the interstitial fluid and the solid skeleton to the overall time-dependent behavior of tendons based on a single mechanical test. For this purpose, the capabilities of three different time-dependent models (a viscoelastic, a poroelastic and a poroviscoelastic) were investigated in the modeling of the experimental behavior obtained from semi-confined compression with stress relaxation tests transverse to collagen fibers. The main achieved result points out that the poroviscoelastic model was the only one capable to characterize both the experimental responses of the force and volume changes of the tissue samples. Moreover, further analysis of this model shows that while the kinematics of the sample are mainly governed by the fluid flow (pore pressure contribution of the model), the behavior intrinsically associated with the viscoelastic solid skeleton makes a significant contribution to the experimental force response. This study reinforces the importance of taking both the experimental kinematics and kinetics of tendon tissues into account during the constitutive characterization procedure.

Rheological and Viscoelastic Analysis of Hybrid Formulations for Topical Application.

The rheological and viscoelastic properties of hybrid formulations composed of vehicles designed for cutaneous topical application and loaded with ultradeformable liposomes (UDL) were assessed. UDL were selected for their established ability to transport both lipophilic and hydrophilic compounds through the skin, and are applicable in pharmaceuticals and cosmetics. Formulations underwent flow analysis and were fitted to the Herschel-Bulkley model due to their prevalent non-Newtonian behavior in most cases. Linear viscoelastic regions (LVR) were identified, and G' and G″ moduli were determined via frequency sweep steps, considering the impact of temperature and aging. The formulations exhibited non-Newtonian behavior with pseudoplastic traits in most cases, with UDL incorporation inducing rheological changes. LVR and frequency sweep tests indicated predominantly elastic solid behavior, with G' higher than G″, at different temperatures and post-production times. Tan δ values also illustrated a predominant solid-like behavior over liquid. This study provides pivotal insights into the rheological and viscoelastic features of topical formulations, emphasizing the crucial role of meticulous vehicle and formulation selection when incorporating UDL or analogous liposomal drug delivery systems.

Nonlinear viscoelastic model for time-dependent mechanical characterization of PMMA acrylic bone cements.

Acrylic bone cement materials are widely used in prosthetic implants, orthopaedics and others trauma surgery. From the mechanical constitutive behaviour viewpoint, experimental analyses have provided ample evidence that such materials exhibit time-dependent properties. In this context, this work addresses the formulation of a nonlinear viscoelastic model for the behaviour of PMMA bone cements under compressive creep loading. Relying upon experimental data available for four PMMA bone cement types, a nonlinear Burgers-like rheological model is formulated and related parameters calibrated for the mechanical description of the time-dependent behaviour of these materials under isothermal conditions and one dimensional setting. The proposed model reveals relevant in reproducing both instantaneous and delayed properties of studied PMMA bone cements.

Baru oil (Dipteryx alata vog.) applied in the formation of O/W nanoemulsions: A study of physical-chemical, rheological and interfacial properties.

The oil extracted from baru (Dipteryx alata Vog.) seeds is in bioactive compounds and it presents potential to be used in food and cosmetic industries. Therefore, this study aims to provide insights into the stability of baru oil-in-water (O/W) nanoemulsions. For this purpose, the effects of the ionic strength (0, 100 and 200 mM), pH (6, 7 and 8), and storage time (28 days) on the kinetic stability of these colloidal dispersions were evaluated. The nanoemulsions were characterized in terms of interfacial properties, rheology, zeta potential (ζ), average droplet diameter, polydispersity index (PDI), microstructure, and creaming index. In general, for samples, the equilibrium interfacial tension ranged from 1.21 to 3.4 mN.m-1, and the interfacial layer presented an elastic behavior with low dilatational viscoelasticity. Results show that the nanoemulsions present a Newtonian flow behavior, with a viscosity ranging from 1.99 to 2.39 mPa.s. The nanoemulsions presented an average diameter of 237-315 nm with a low polydispersity index (<0.39), and a ζ-potential ranging from 39.4 to 50.3 mV after 28 days of storage at 25 °C. The results obtained for the ζ-potential suggest strong electrostatic repulsions between the droplets, which is an indicative of relative kinetic stability. In fact, macroscopically, all the nanoemulsions were relatively stable after 28 days of storage, except the nanoemulsions added with NaCl. Nanoemulsions produced with baru oil present a great potential to be used in the food, cosmetic, and pharmaceutical industries.

Characterisation of the soft tissue viscous and elastic properties using ultrasound elastography and rheological models: validation and applications in plantar soft tissue assessment.

Objective. The mechanical behaviour of soft tissue is influenced by its elastic and viscous characteristics. Therefore, the aim of this study was to develop a validated method to characterise the viscoelastic properties of soft tissues based on ultrasound elastography data.Approach. Plantar soft tissue was chosen as the tissue of interest, and gelatine-phantoms replicating its mechanical properties were manufactured for validation of the protocol. Both plantar soft tissue and the phantom were scanned using Reverberant shear wave ultrasound (US) elastography at 400-600 Hz. Shear wave speed was estimated using the US particle velocity data. The viscoelastic parameters were extracted by fitting the Young's modulus as a function of frequency derived from the constitutive equations of the eight rheological models (four classic and their fractional-derivative versions) to the shear wave dispersion data. Furthermore, stress-time functions derived from the eight rheological models were fitted to the phantom stress-relaxation data.Main results. The viscoelastic parameters estimated using elastography data based on the fractional-derivative (FD) models, compared to the classic models, were closer to those quantified using the mechanical test. In addition, the FD-Maxwell and FD-Kelvin-Voigt models showed to more effectively replicate the viscoelastic behaviour of the plantar soft tissue with minimum number of model parameters (R2= 0.72 for both models) . Hence the FD-KV and FD-Maxwell models can more effectively quantify the viscoelastic characteristics of the soft tissue compared to other models.Significance. In this study, a method for mechanical characterisation of the viscoelastic properties of soft tissue in ultrasound elastography was developed and fully validated. An investigation into the most valid rheological model and its applications in plantar soft tissue assessment were also presented. This proposed approach for the characterisation of viscous and elastic mechanical properties of soft tissue has implications in assessing the soft tissue function where those can be used as markers for diagnosis or prognosis of tissue status.

Multivariate Analysis on the Properties of Intact Cereal Kernels and Their Association with Viscoelasticity at Different Moisture Contents.

The viscoelastic properties of cereal kernels are strongly related to their quality, which can be applied to the development of a more selective and objective classification process. In this study, the association between the biophysical and viscoelastic properties of wheat, rye, and triticale kernels was investigated at different moisture contents (12% and 16%). A uniaxial compression test was performed under a small strain (5%), and the increase in viscoelasticity at 16% moisture content corresponded to proportional increases in biophysical properties such as the appearance and geometry. The biophysical and viscoelastic behaviors of triticale were between those of wheat and rye. A multivariate analysis showed that the appearance and geometric properties significantly influenced kernel features. The maximum force showed strong correlations with all viscoelastic properties, and it can be used to distinguish between cereal types and moisture contents. A principal component analysis was performed to discriminate the effect of the moisture content on different types of cereals and to evaluate the biophysical and viscoelastic properties. The uniaxial compression test under a small strain and the multivariate analysis can be considered a simple and non-destructive tool for assessing the quality of intact cereal kernels.

Methodologies and models for measuring viscoelastic properties of cancer cells: Towards a universal classification.

Different methods and several physical models exist to study cell viscoelasticity with the atomic force microscope (AFM). In search of a robust mechanical classification of cells through AFM, in this work, viscoelastic parameters of the cancer cell lines MDA-MB-231, DU-145, and MG-63 are obtained using two methodologies; through force-distance and force-relaxation curves. Four mechanical models were applied to fit the curves. The results show that both methodologies agree qualitatively on the parameters that quantify elasticity but disagree on the parameters that account for energy dissipation. The Fractional Zener (FZ) model represents well the information given by the Solid Linear Standard and Generalized Maxwell models. The Fractional Kelvin (FK) model concentrates the viscoelastic information mainly in two parameters, which could be an advantage over the other models. Therefore, the FZ and FK models are proposed as the basis for the classification of cancer cells. However, more research using these models is needed to obtain a broader view of the meaning of each parameter and to be able to establish a relationship between the parameters and the cellular components.

Design as strategy for evaluation of the mechanical properties of binary mixtures composed of poly(methyl vinyl ether-alt-maleic anhydride) and Pluronic F127 for biomedical applications.

The synergism between thermoresponsive and bioadhesive polymers can lead to the optimization of materials with enhanced mechanical and bioadhesive properties. Quality by Design can assure the understanding and control of formulation variables. In this approach, Design of Experiment has been widely utilized as an important strategy. Poly(methyl vinyl ether-alt-maleic anhydride) (PVMMA) is a bioadhesive polymer and Pluronic F127 (PF127) shows thermoresponsiveness. The association of these two polymers has been poorly investigated. The aim of this work was to study the mechanical, bioadhesive and rheological properties of polymer mixtures composed of PVMMA and PF127, in order to select the best conditions and formulations for biomedical applications. Textural properties (hardness, compressibility, adhesiveness, cohesiveness and elasticity), softness index, bioadhesion and rheological characteristics (flow and viscoelasticity) showed that 17.5-20% (w/w) PF127-polymer mixtures displayed improved values of the parameters. However, the rheological interaction parameter showed low synergism, due to the polymers' characteristics and system organization. The formulations displayed gelation temperatures suitable for administration, with improved bioadhesive properties mainly at 34 °C and suggests the formulations can be used for biomedical applications. DoE constituted an important tool to investigate these systems showing the main effects that significantly influence the binary mixtures.

Numerical Simulation of Rheological Models for Complex Fluids Using Hierarchical Grids.

In this work, we implement models that are able to describe complex rheological behaviour (such as shear-banding and elastoviscoplasticity) in the HiGTree/HiGFlow system, which is a recently developed Computational Fluid Dynamics (CFD) software that can simulate Newtonian, Generalised-Newtonian and viscoelastic flows using finite differences in hierarchical grids. The system uses a moving least squares (MLS) meshless interpolation technique, allowing for more complex mesh configurations while still keeping the overall order of accuracy. The selected models are the Vasquez-Cook-McKinley (VCM) model for shear-banding micellar solutions and the Saramito model for viscoelastic fluids with yield stress. Development of solvers and numerical simulations of inertial flows of these models in 2D channels and planar-contraction 4:1 are carried out in the HiGTree/HiGFlow system. Our results are compared with those predicted by two other methodologies: the OpenFOAM-based software RheoTool that uses the Finite-Volume-Method and an in-house code that uses the Vorticity-Velocity-Formulation (VVF). We found an excellent agreement between the numerical results obtained by these three different methods. A mesh convergence analysis using uniform and refined meshes is also carried out, where we show that great convergence results in tree-based grids are obtained thanks to the finite difference method and the meshless interpolation scheme used by the HiGFlow software. More importantly, we show that our methodology implemented in the HiGTreee/HiGFlow system can successfully reproduce rheological behaviour of high interest by the rheology community, such as non-monotonic flow curves of micellar solutions and plug-flow velocity profiles of yield-stress viscoelastic fluids.

Parameter Identification of Fractional Index Viscoelastic Model for Vegetable-Fiber Reinforced Composite.

In the present work, parameters for adapting the behavior of the uniaxial three-element viscoelastic constitutive model with integer and fractional index derivatives to the mechanical evolution of an epoxy-composite material reinforced with long random henequen fibers, were determined. Cyclic loading-unloading with 0.1%, 0.2%, 0.3%, …, 1.0% controlled strain and staggered fluency experiments at 5 MPa, 10 MPa, and 15 MPa constant tension were performed in stages, and the obtained data were used to determine and validate the model's parameter values. The Inverse Method of Identification was used to calculate the parameters, and the Particle Swarm Optimization (PSO) method was employed to achieve minimization of the error function. A comparison between the simulated uniaxial results and the experimental data is demonstrated graphically. There exists a strong dependence between properties of the composite and the fiber content (0 wt%, 9 wt%, 14 wt%, 22 wt%, and 28 wt% weight percentage fiber/matrix), and therefore also of the model parameter values. Both uniaxial models follow the viscoelastic behavior of the material and the fractional index version presents the best accuracy. The latter method was noted to be adequate for determination of the aforementioned constants using non-large experimental data and procedures that are easy to implement.

Estimation of the Effects of CO2 and Temperature on the Swelling of PS-CO2 Mixtures at Supercritical Conditions on Rheological Testing.

The use of supercritical CO2 as a blowing agent for polymeric foams instead of traditional blowing agents has been a trend in recent years. To achieve the final desired properties of the polymeric foams, the rheological behavior of the material needs to be reliable. The polymer swelling in the samples for rheological testing affects the results of the viscoelastic properties of the material. This study proposes a new testing methodology to control the accuracy and repeatability of the rheological characterization for PS-SCO2 samples. To develop this methodology, three polystyrene resins with different molecular weight distribution were studied at three temperatures (170, 185 and 200 °C) and three pressures (0.1 MPa, 6.89 MPa and 13.78 MPa). The CO2 concentration was estimated and used in the Sanchez-Lacombe Equation of State (SLEOS) to determine the polymer swelling, as it affects the dimensions of specimens tested in high-pressure rheometers. The correction factors provided a consistent trend in the viscosity with respect to temperature and a decrease of up to 50% in the standard deviation. The results of this study are crucial for an accurate measurement of viscoelastic properties by parallel-plate rheometry.

Force-Displacement Analysis in Diaphragm-Embedded Fiber Bragg Grating Sensors.

This paper presented the force and displacement analyses of a diaphragm-embedded fiber Bragg grating (FBG) sensor. In the first step, a numerical analysis (via finite element method) was performed considering linear elastic materials, where there is a linear variation on the strain in the optical fiber for both displacement and force (or pressure). In the second step, the experimental analysis was performed using two approaches: (i) controlling the displacement applied in the diaphragm-embedded FBG (while the force is also measured). (ii) Controlling the force applied in the sensor (also with the measurement of the displacement). Results showed reflected optical power variations and wavelength shift following the application of displacement and force. The sensitivities of both wavelength shift and optical power were different (and non-proportional) when displacement and force were compared. However, a higher correlation, determination coefficient (R2) of 0.998, was obtained in the analysis of the wavelength shift as a function of the displacement, which indicated that the strain transmission in the optical fiber is directly related to the strain in the diaphragm, whereas the force has an indirect relation with the strain and depends on the material features. Then, the possibility of simultaneous estimation of force and displacement was investigated, where the linear relation of both parameters (displacement and force) with the wavelength shift and the optical power were obtained in a limited range of displacement and force. In this range, root mean squared errors of 0.37 N and 0.05 mm were obtained for force and displacement, respectively. In addition, the force variation with a step displacement input also shows the possibility of using the proposed FBG device for the characterization of the materials' viscoelastic features such as phase delay, creep, and stress relaxation, which can be employed for in situ characterization of different viscoelastic materials.

Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications.

Dermal wound healing relies on the properties of the extracellular matrix (ECM). Thus, hydrogels that replicate skin ECM have reached clinical application. After a dermal injury, a transient, biodegradable fibrin clot is instrumental in wound healing. Human plasma, and its main constituent, fibrin would make a suitable biomaterial for improving wound healing and processed as hydrogels albeit with limited mechanical strength. To overcome this, plasma-agarose (PA) composite hydrogels have been developed and used to prepare diverse bioengineered tissues. To date, little is known about the influence of variable agarose concentrations on the viscoelastic properties of PA hydrogels and their correlation to cell biology. This study reports the characterization of the viscoelastic properties of different concentrations of agarose in PA hydrogels: 0 %, 0.5 %, 1 %, 1.5 %, and 2 % (w/v), and their influence on the cell number and mitochondrial activity of human dermal fibroblasts. Results show that agarose addition increased the stiffness, relaxation time constants 1 (τ1) and 2 (τ2), and fiber diameter, whereas the porosity decreased. Changes in cell metabolism occurred at the early stages of culturing and correlated to the displacement of fast (τ1) and intermediate (τ2) Maxwell elements. Fibroblasts seeded in low PA concentrations spread faster during 14 d than cells cultured in higher agarose concentrations. Collectively, these results confirm that PA viscoelasticity and hydrogel architecture strongly influenced cell behavior. Therefore, viscoelasticity is a key parameter in the design of PA-based implants.

Study of nonlinear viscoelastic mechanical properties of degenerated intervertebral discs / Estudio de las propiedades mecánicas viscoelásticas no lineales de discos intervertebrales degenerados

SUMMARY: Biomechanical factors are important factors in inducing intervertebral disc degeneration, in this paper, the nonlinear viscoelastic mechanical properties of degenerated intervertebral discs were analyzed experimentally. Firstly, the loading and unloading curves of intervertebral discs before and after degeneration at different strain rates were compared to analyze the changes of their apparent viscoelastic mechanical properties; The internal stress/strain distribution of the disc before and after degeneration was then tested by combining digital image technology and fiber grating technology. The results show that the intervertebral disc is strain-rate- dependent whether before or after degeneration; The modulus of elasticity and peak stress of the degenerated disc are significantly reduced, with the modulus of elasticity dropping to 50 % of the normal value and the peak stress decreasing by about 55 %; Degeneration will not change the distribution of the overall internal displacement of the intervertebral disc, but has a greater impact on the superficial and middle AF; The stress in the center of the nucleus pulposus decreases, and the stress in the outer AF increases after degeneration. Degeneration has a great impact on the nonlinear viscoelastic mechanical properties of intervertebral disc, which has reference value for the mechanism, treatment and prevention of clinical degenerative diseases.

RESUMEN: Los factores biomecánicos son importantes en la inducción de la degeneración del disco intervertebral. En este estudio se analizaron experimentalmente las propiedades mecánicas viscoelásticas no lineales de los discos intervertebrales degenerados. En primer lugar se compararon las curvas de carga y descarga de los discos intervertebrales, antes y después de la degeneración, a diferentes velocidades de deformación para analizar los cambios aparentes de sus propiedades mecánicas viscoelásticas. La distribución interna de tensión/deformación del disco antes y después de la degeneración se probó luego combinando tecnología de imagen digital y tecnología de rejilla de fibra. Los resultados mostraron que el disco intervertebral depende de la velocidad de deformación antes o después de la degeneración; El módulo de elasticidad y la tensión máxima del disco degenerado se reducen significativamente, cayendo el módulo de elasticidad al 50 % del valor normal y la tensión máxima disminuyendo en aproximadamente un 55 %; La degeneración no cambiará la distribución del desplazamiento interno general del disco intervertebral, pero tiene un mayor impacto en la FA superficial y media; El estrés en el centro del núcleo pulposo disminuye y el estrés en el FA externo aumenta después de la degeneración. La degeneración tiene un gran impacto en las propiedades mecánicas viscoelásticas no lineales del disco intervertebral, que tiene valor de referencia para el mecanismo, tratamiento y prevención de enfermedades clínicas degenerativas.

Characterization of Viscoelastic Poisson's Ratio of Engineering Elastomers via DIC-Based Creep Testing.

New data of creep and viscoelastic Poisson's ratio, ν(t), of five engineering elastomers (Ethylene Propylene-Diene Monomer, Flouroelastomer (Viton®), nitrile butadiene rubber, silicone rubber and neoprene/chloroprene rubber) at different stress (200, 400 and 600 kPa) and temperature (25, 50 and 80 °C) are presented. The ν(t) was characterized through an experimental methodological approach based on creep testing (30 min) and strain (axial and transverse) measurements by digital image correlation. Initially, creep behavior in axial and transverse directions was characterized for each elastomer and condition, and then each creep curve was fitted to a four-element creep model to obtain the corresponding functions. The obtained functions were used to estimate ν(t) for prolonged times (300 h) through a convolution equation. Overall, the characterization was achieved for the five elastomers results exhibiting ν(t) increasing with temperature and time from about 0.3 (for short-term loading) to reach and stabilize at about 0.48 (for long-term loading).

Development of new eggplant spread product: A rheological and chemical characterization.

Eggplant (Solanum melongena) is an important vegetable of the Nightshade family with high demand due to its nutritional value and medicinal properties. The principal objective of this work was to develop and standardize a spread eggplant paste (SEP) with the addition of guar gum and evaluate its physicochemical, bromatological, sensory, and rheological characteristics. A two-factor factorial design with three levels was used for the formulation, evaluating the percentage of guar gum and oil. Flow curves in steady-state and small-amplitude oscillatory shear tests were performed to evaluate the rheological properties of the pastes. Sensorial analysis was performed using descriptive analysis. The standardized eggplant showed no signs of phase separation being stable during storage. Samples presented a non-Newtonian shear-thinning behavior described by Ostwald de-Waele model (R2 > 0.969). The products exhibit more elastic than viscous behavior, with a higher storage modulus than loss modulus in the evaluated frequency range, where the modules could be well described by a power function of the oscillatory frequency. The sensory evaluation revealed that the product color, odor, taste, and spreadability were acceptable, being an alternative for the transformation and agro-industrial use of eggplant for production chain development.

Kinetic stability of the oil-in-water emulsions and dynamic interfacial properties of mixtures of sucrose esters and polysaccharides.

This article presents a study of the interfacial properties of oil-in-water emulsions containing sugar esters and polysaccharides. Sucrose fatty acid esters were synthesized using immobilized Candida antarctica lipase B. A yield of 53.4% was obtained using 2-methyl-2-butanol and 1:3 M ratio of sucrose:stearic acid. Equilibrium surface tension was 45 mN/m and low critical micellar concentration (CMC) value was obtained (ca. 10 mg/mL), characteristic of non-ionic surfactant. The interfacial properties of mixtures of sucrose esters and polysaccharides, at the oil-water interface were determined using a pendant drop tensiometer. Addition of polysaccharides increased the interfacial tension. Studies of interfacial viscoelasticity showed that the films were predominantly elastic. The presence of polysaccharides in emulsions resulted in flocculated droplets. All the emulsions presented great stability along 28 days with no creaming formation.

Coffee Cherry Pulp by-Product as a Potential Fiber Source for Bread Production: A Fundamental and Empirical Rheological Approach.

Effects of substituting of wheat flour with coffee cherry pulp powder (CCPP) (coffee by-product as fiber source) at 0, 1.2, 2.3, and 4.7% dry basis (0, 1.25, 2.5, and 5% wet basis) on dough and gluten rheological properties and baking quality were investigated. Rheological properties were analyzed during mixing, compression recovery, and creep-recovery. A rheological approach was adopted to study the viscoelasticity of dough enriched with fiber. The data obtained were analyzed with the Kelvin-Voigt model and the parameters were correlated to bread volume and crumb firmness to assess the effect of incorporating CCPP. A decrease in gluten's elastic properties was attributed to the water-binding and gelling properties of CCPP. Stiffness of dough and crumb firmness increased as the level of CCPP increased and bread volume decreased. Stiffer dough corresponded with lower compliance values and higher steady state viscosity compared to the control. A follow-up study with 5% CCPP and additives is recommended to overcome the reduction in elastic recovery and bread volume.