Effects of innovative reinforced concrete slit shaft configuration on seismic performance of elevated water tanks.

Elevated water tanks are considered crucial infrastructure due to their significant role in supporting essential services. A strong ground motion may result in a failure or significant damage to a reinforced concrete shaft of an elevated water tank because hysteric energy dissipation is limited to the formation of plastic hinges at the base of the shaft, while the nonlinear properties of the rest of the shaft remain underutilised. The innovative system of assembling RC shafts for elevated water tanks using a slit wall technique was developed to enhance energy dissipation along with the shaft height by introducing slit zones. The comparative nonlinear dynamic analysis between three-dimensional models of elevated water tanks with different shaft diameters and heights was conducted using SAP2000 software. The results of elevated water tanks with slit and solid reinforced concrete shafts were compared. The research findings showed that during a seismic event, the slit zones increased the ductility of the shaft, reduced stress concentration in the lower part of the shaft, and provided uniform stress distribution throughout the shaft's height. The effect of the innovative system is especially noticeable in the elevated water tanks with tall and slender shafts.

Enhancing Thermal Efficiency in Water Storage Tanks Using Pigmented Recycled Concrete.

The present work investigated the manufacture of elements such as water tanks from recycled concrete for applications where industries require water heating. This proposal leverages precast rejects for recycled concrete and incorporates colouring pigments. It is expected to contribute to the circularity of construction materials (due to the total replacement of natural aggregates by recycled aggregates) as well as to energy and emissions savings, which are attributed to improved thermal performance driven by the thermal behaviour that the coloration pigment gives to the manufactured concrete elements. To assess the efficacy of the proposed solution, on the one hand, mechanical tests were carried out in tensile, compression and modulus of elasticity, which showed a suitable concrete dosage for HA-30 structural concrete. Simultaneously, in search for a material that would increase the internal temperature of the tanks, thermal tests were carried out in a controlled laboratory environment on samples with different percentages of pigment, and an optimum concentration of 1% was obtained. It was also found that the thermal conductivity remained almost unaffected. Finally, two water tank prototypes were manufactured and tested under real environmental conditions: one with the optimised pigment concentration solution and other (the reference tank) without pigment. The results revealed that the colourised tank with the optimal concentration resulted in an average water temperature increase of 2 °C with respect to the reference tank. Finally, the economic and environmental benefits of this temperature increase were studied for industrial processes requiring water heating with a potential saving of 8625 kWh per month.

Quadriceps and gluteus medius activity during stable and unstable loading exercises in athletes. A cross-sectional study.

The aim of this study was to evaluate the muscle activation of the vastus medialis, vastus lateralis, and gluteus medius during different strength and stability exercises with a water tank compared with a sandbag. A cross-sectional study was conducted in the Functional Anatomy Laboratory, and the sample consisted of 28 athletes. The main outcome measures were surface electromyography (dependent variable), water tank and sandbag, and exercise type (independent variables): Isometric Single Leg Stance (ISLS), One Leg Deadlift (OLDL), Front Rack Forward Lunge (FRFL), and Lateral Lunge (LL). Repeated measures analysis of variance (ANOVA) revealed a significant Group × Time interaction in gluteus medius root mean square (RMS) (F = 14.198, p < 0.001, ŋ2 = 0.35), vastus lateralis RMS (F = 24.374, p < 0.001, ŋ2 = 0.47), and vastus medialis RMS (F = 27.261, p < 0.001, ŋ2 = 0.50). In the between-group analysis, statistically significant differences were observed in gluteus medius RMS in the ISLS: 28.5 ± 15.8 water tank and 20.8 ± 12.6 sandbag (p < 0.001, ŋ2 = 0.08) and OLDL: 29.7 ± 13.3 water tank and 26.5 ± 13.1 sandbag (p < 0.001, ŋ2 = 0.01). In vastus lateralis in ISLS: 30.4 ± 37.6 water tank and 19.0 ± 26.7 sandbag (p < 0.001, ŋ2 = 0.03). In vastus medialis in ISLS: 14.2 ± 13.0 water tank and 7.0 ± 5.6 sandbag (p < 0.001, ŋ2 = 0.12), OLDL: 21.5 ± 16.9 water tank and 15.5 ± 10.7 sandbag (p = 0.002, ŋ2 = 0.04), and LL: 51.8 ± 29.6 water tank and 54.3 ± 29.3 sandbag (p = 0.017, ŋ2 = 0.00). These results confirm significantly greater activation of the gluteus medius and vastus medialis in the ISLS and OLDL exercises, and of the vastus lateralis in the water tank ISLS exercise. However, the vastus medialis shows greater activation in the LL exercise.

The dispersal of dinoflagellate cyst caused by international ships under repair conditions: a potential invasion risk to the Yangtze River Estuary, China.

The foreign dinoflagellate cysts in ships' ballast water tank sediments (BWTS) can be viable for a long time under hostile storage conditions. It is crucial to understand the detailed mechanisms of harmful biological invasions in estuary ecosystems. To study the relationship between the abundance of dinoflagellate cysts and environmental factors, cyst assemblages were analyzed in 7 sediment samples, collected from one international commercial ship that arrived in Shanghai in August 2020. Twenty-three dinoflagellate cyst taxa were identified in 5 groups, including autotrophic (9) and heterotrophic (14) species. Distribution of dinoflagellate cysts in the different ballast water tanks is heterogeneous. Dinoflagellate cysts in BWTS of the repaired ship were dominated by Scrippsiella acuminata, Protoperidinium leonis, Protoperidinium oblongum, Lingulodinium polyedra, Alexandrium tamarense/A. catenella, Protoperidinium pentagonum, and Protoperidinium subinerme. The abundance of the dinoflagellate cysts in each tank ranged from 80.69 to 330.85 cysts g-1 DS (dry sediment). Multivariate statistical analyses revealed that the variation in cysts from different tanks had positive correlations with total nitrogen (TN), total phosphorus (TP), and pH and negative correlations with total organic carbon (TOC) except for sample TK5. For germination of dinoflagellate cysts in BWTS, 12 species were germinated in 40 days and cysts of potentially toxic dinoflagellate species were more abundant than those of non-toxic species. Results show that potentially viable and harmful/toxic dinoflagellate cysts are present in BWTS of ships arriving in Shanghai, China. Consequently, knowledge obtained in this study can be valuable for further managing of potential biological invasion of the Yangtze River Estuary.

[Water tank scale: a reliable method for assessing motor function after spinal cord injury in rats].

OBJECTIVE: To analyze the reliability of the Water Tank Scale for assessing recovery of motor function after spinal cord injury (SCI) in rats. METHODS: Thirty-six adult female SD rats were randomly divided into SCI and sham-operated groups (n= 18). The recovery of the hind limb motor function was assessed using Water Tank scoring, BBB scoring, and motor-evoked potentials (MEP) at 1, 3, 5, 7, 14 and 21 days after SCI. MEP was used as the gold standard for analyzing and comparing differences between the two scoring methods. RESULTS: The Water Tank scores of the rats were significantly higher than the BBB scores on day 3 (0.22±0.43 vs 0, P < 0.05) and also on days 5, 7 and 14 after SCI (0.67±0.49 vs 0.11±0.32, 4.33±1.19 vs 2.83±1.04, 8.61± 1.20 vs 7.06±1.0, P < 0.01). On day 21 after SCI, the scores of the Water Tank Scale of the rats did not significantly differ from the BBB scores (14.78±1.06 vs 14.50±1.47, P>0.05). Neurophysiological monitoring showed that both the Water Tank score and BBB score were significantly correlated with MEP latency, but the Water Tank score had a greater correlation coefficient with MEP latency (r=-0.90). CONCLUSION: Compared with the BBB scale, Water Tank scoring allows more objective and accurate assessment of functional recovery of the spinal cord in early stages following SCI in rats, and can thus be used as a reliable method for assessing functional recovery of the hind limbs in rat models of acute SCI.

Peroneal muscle activity during stable and unstable load exercises. A cross-sectional study.

OBJECTIVE: To evaluate the muscle activity of the peroneus longus during the execution of different unstable load exercises compared to stable load exercises. DESIGN: Cross-sectional study. SETTING: Functional Anatomy Laboratory. PARTICIPANTS: 28 healthy athletes. MAIN OUTCOME MEASURES: Surface electromyography (sEMG), unstable load (water tank), Stable Load (Sandbag), type of exercise (Isometric single leg stance, single-leg Romanian deadlift, front rack forward lunge and lateral lunge). RESULTS: The repeated measures ANOVA revealed significant Group ∗ Time interaction in Root Mean Square (RMS) (F = 51.307, P < 0.001, ŋ2 = 0.70). In the between-group analysis there were statistically significant differences in RMS isometric single leg stance in favor to unstable load (P < 0.001, ŋ2 = 0.07). In the within-group analysis of RMS statistically significant differences were found in the stable load group between isometric single leg stance and single-leg Romanian deadlift (P < 0.001, ŋ2 = 0.05). CONCLUSIONS: Single leg stance exercise with unstable load showed higher peroneus muscle activity than stable load. However, no peroneus muscle activity differences were found between unstable and stable loads for single-leg Romanian deadlift, frontal rack forward lunge and lateral lunge. Single-leg Romanian deadlift with stable load showed higher peroneus muscle activity than isometric single leg stance with stable load.

Water tank scale: a reliable method for assessing motor function after spinal cord injury in rats / 南方医科大学学报

OBJECTIVE@#To analyze the reliability of the Water Tank Scale for assessing recovery of motor function after spinal cord injury (SCI) in rats.@*METHODS@#Thirty-six adult female SD rats were randomly divided into SCI and sham-operated groups (n= 18). The recovery of the hind limb motor function was assessed using Water Tank scoring, BBB scoring, and motor-evoked potentials (MEP) at 1, 3, 5, 7, 14 and 21 days after SCI. MEP was used as the gold standard for analyzing and comparing differences between the two scoring methods.@*RESULTS@#The Water Tank scores of the rats were significantly higher than the BBB scores on day 3 (0.22±0.43 vs 0, P < 0.05) and also on days 5, 7 and 14 after SCI (0.67±0.49 vs 0.11±0.32, 4.33±1.19 vs 2.83±1.04, 8.61± 1.20 vs 7.06±1.0, P < 0.01). On day 21 after SCI, the scores of the Water Tank Scale of the rats did not significantly differ from the BBB scores (14.78±1.06 vs 14.50±1.47, P>0.05). Neurophysiological monitoring showed that both the Water Tank score and BBB score were significantly correlated with MEP latency, but the Water Tank score had a greater correlation coefficient with MEP latency (r=-0.90).@*CONCLUSION@#Compared with the BBB scale, Water Tank scoring allows more objective and accurate assessment of functional recovery of the spinal cord in early stages following SCI in rats, and can thus be used as a reliable method for assessing functional recovery of the hind limbs in rat models of acute SCI.

Identificación de amebas de vida libre en tanques de almacenamiento de agua de edificios del área metropolitana de Ambato / Identification of free-living amoebas in water storage tanks of buildings in the metropolitan area of Ambato

Naegleria fowleri, Acanthamoeba spp. y Balamuthia mandrillaris, son especies de ameba de vida libre (AVL) que causan enfermedades en el sistema nervioso central grave y puede ser fatal en humanos y otros animales. Todos los géneros de AVL tienen dos etapas: quiste y trofozoíto. Los trofozoítos se dividen por fisión binaria, se alimentan activamente y es la etapa infecciosa del organismo. En el medio ambiente, los acanthopodios permiten que los trofozoítos se adhieran a las superficies de las bacterias, hongos, algas y detritos en los que se encuentran su alimento. Acanthamoeba spp. Parecen mantener una relación endosimbiótica con algunas de las bacterias que consumen. Muchas de estas bacterias, como la Legionella, Pseudomonas, Vibrio y algunas micobacterias parecen adquirir factores de virulencia mientras están viviendo dentro de las amebas, lo que les permite sobrevivir y prosperar dentro de otras células fagocíticas (por ejemplo, macrófagos humanos). La tenacidad de AVL para proliferar en ecosistemas hostiles y sobrevivir a la desecación o desinfección hace de estos organismos una fuente potencial transmitida por el agua, por los alimentos y el aire. Conocidos por su ubicuidad, los AVL han sido aislados de diversas matrices ambientales, como suelo, agua dulce y salobre, aguas residuales, aguas termales, polvo y otros. En función de lo antes comentado, este trabajo tiene como objetivo identificar amebas de vida libre en tanques de almacenamiento de agua de edificios del área metropolitana de la ciudad de Ambato (Ecuador), con el fin de aplicar soluciones correctivas en cualquiera de sus puntos. Según el sitio de muestreo, el género de AVL identificado con mayor frecuencia en la superficie fue Balamuthia sp (70,59%) y Acanthamoeba sp., con un 37,78 %. El fondo estuvo caracterizado por Naegleria sp. con un 44,44%(AU)

Naegleria fowleri, Acanthamoeba spp. and Balamuthia mandrillaris are species of free-living amoeba (FLA) that cause severe central nervous system disease and can be fatal in humans and other animals. All genera of FLA have two stages: cyst and trophozoite. Trophozoites divide by binary fission, actively feed, and is the infective stage of the organism. In the environment, acanthopods allow trophozoites to adhere to the surfaces of bacteria, fungi, algae, and detritus on which their food is found. Acanthamoeba spp. they appear to maintain an endosymbiotic relationship with some of the bacteria they consume. Many of these bacteria, such as Legionella, Pseudomonas, Vibrio, and some mycobacteria appear to acquire virulence factors while living inside amoebas, allowing them to survive and thrive inside other phagocytic cells (eg, human macrophages). FLA's tenacity to proliferate in hostile ecosystems and survive desiccation or disinfection makes these organisms a potential waterborne, foodborne, and airborne source. Known for their ubiquity, FLAs have been isolated from various environmental matrices, such as soil, fresh and brackish water, wastewater, hot springs, dust, and others. Based on the aforementioned, this work aims to identify free-living amoebas in water storage tanks of buildings in the metropolitan area of the city of Ambato (Ecuador), in order to apply corrective solutions at any of its points. According to the sampling site, the FLA genus most frequently identified on the surface was Balamuthia sp (70.59%) and Acanthamoeba sp., with 37.78 %. The background was characterized by Naegleria sp. with 44.44%(AU)

New actions and land uses in the historical heritage: the case study of one of the oldest underground water tanks in Madrid (Spain).

The preservation of architectural heritage in Europe is generally associated with the recovery of monumental buildings such as churches and castles, leaving behind a high amount of other historical constructions of civil and industrial architecture that exist in an old country like Spain. High efforts have been devoted by professionals from very different fields aiming to recover and enhance engineering works such as bridges, but the particular case of hydraulic works deserves special attention because it has remained hidden for centuries and sometimes continues to be ignored by society and many institutions. This type of architectural heritage, in the form of underground deposits that represents the case under study in this research, occupies a large area in cities such as Madrid and must be preserved to relieve the pressures of adapting them to accommodate other uses of the land that they occupy. In this paper, the complexity of material characterization and calculation of the Mayor water tank in Madrid (Spain), as an example of this type of construction, is presented. This reservoir currently supplies drinking water to a large part of the city and can accommodate a park on its surface without altering an original 150-year-old structure. As the main conclusion of this research, and in the absence of detailed studies of pathologies, tests, characterization of materials and the terrain, it is recommended to avoid loads greater than 30 kN/m2 in the existing structure, hence the current stresses are not substantially exceeded. This is a recommendation to preserve the historical heritage of this structure against economic and political perspectives that push for a substantial change in its use. This research is an example that can easily be extrapolated to other similar historical infrastructures aiming to guarantee their prevalence in the future.

Plant growth-promoting traits of yeasts isolated from the tank bromeliad Vriesea minarum L.B. Smith and the effectiveness of Carlosrosaea vrieseae for promoting bromeliad growth.

Yeasts can play important roles in promoting plant growth; however, little information is available in this regard for yeasts in water of bromeliad tanks. Here, we characterize the ability of 79 yeast isolates from tank bromeliad Vriesea minarum, an endangered species, to solubilize phosphate, secrete siderophores, and synthesize indole-3-acetic acid (IAA). The results showed that 67.8% of all assayed yeast isolates mobilized inorganic phosphate; 40.0% secreted siderophores; and 89.9% synthetized IAA and IAA-like compounds. Among the species studied, Carlosrosaea vrieseae UFMG-CM-Y6724 is highlighted for producing IAA (76.1 µg mL-1) and siderophores, and solubilizing phosphate. In addition, evaluation of the effects of filtrate containing IAA-like compounds produced by the C. vrieseae on the development and photosynthetic performance of V. minarum seedlings found it to improve seedling growth equal to that of commercial IAA. These results demonstrate that C. vrieseae can produce compounds with great potential for future use as biofertilizer agents.

Performance of dispersed oil and suspended sediment during the oil-sediment aggregation process.

Oil-sediment aggregation is an important transport and transformation process of spilled oil, which has been considered as a pathway of spill remediation. This work focused on the individual performance of dispersed oil and sediment during the aggregation process. Dispersion of three oils was first tested and validated in a water tank. An approach of estimating the mass variation of the sediment that has participated in forming the oil-sediment aggregates (OSAs) has been developed by density analysis. Results indicated that the density of the formed OSAs increases during the aggregation. In the context of remediation, it takes longer for sediment to reach equilibrium than for dispersed oil, especially under high mixing energy at a large sediment concentration, which results in the formation of dense OSAs, as well as high aggregation degree and rate. Roncador oil possesses a relatively high capability of capturing sediment to form dense OSAs, especially at an initial sediment concentration of over 150 mg/L. Oil sinking efficiency and the characteristic change rate of aggregated oil mass seem to be proportional to oil dispersion efficiency, and decrease with the mean size of dispersed oil droplets. The process of aggregation can further promote the dispersion of oil into water column. This study also provides fundamental data for the formation kinetics of OSAs.

Metallographic Testing of 19th Century Steel in an Operating Water Tower.

The world's first steel structures were built towards the end of the 19th century. Some of them are still in use today, whereas others are maintained as precious technical heritage. In both cases, there may be a need to assess their technical condition and carry out repairs and reinforcements, which requires an understanding of the properties of the steel used. The few studies that have been undertaken of such steel structures indicate that the properties depend on the history of use. This paper presents the results of metallographic tests of a steel tank in a water tower built in 1884 in Lower Silesia. The chemical composition was consistent with that of the puddled steel used in the 19th century. The carbon content showed significant segregation and ranged from 0.011% to 0.072% mass. As a consequence, a typical microstructure for low-carbon steels (ferritic) was observed, changing locally to ferritic-pearlitic. The tested steel contained a very high content of phosphorus and silicon. The microstructure with numerous slags favoured the formation of surface delamination caused by the corrosion processes. The degree of corrosion of the steel of the tank was also assessed, as well as the type of corrosion inside the tank. Corrosion was favoured by the oxygen concentration cell. The results of the research will be used to assess the potential for continuing tank use and the reinforcements that have been planned. The results presented will add to the somewhat limited research results available for steel dating back to the 19th century, which is still present in many building structures. Such a database is especially needed by those designing technical measures aimed at maintaining these historical structures in good technical condition.

Development of a low-cost six-axis alignment instrument for flexible 2D and 3D ultrasonic probes.

BACKGROUND: The pulse-echo test is used to evaluate the performance of ultrasonic probes before manufacturing ultrasonic systems. However, commercial alignment instruments are very large and use complex programs with long operation times. OBJECTIVE: To develop a low-cost alignment instrument used in the pulse-echo test for evaluating the performance of various 2D and 3D ultrasonic probes. METHODS: The developed alignment instrument can be aligned with the X, Y, Z, azimuth, elevation, and tilt axes with manual structure to support mounting fixtures that hold 2D and 3D ultrasonic probes. Each axis has a manual lever and is designed to have no movement when fixed. In particular, tilt and azimuth directions are designed to move more than 5∘ left and right. RESULTS: The probe mounted in the X, Y, and Z axes can move at above 50 mm. The probe mounted in the azimuth, elevation, and tilt axes can move more than 5∘ in the left and right directions. The pulse-echo test using commercial ultrasonic probes showed maximum error rate of less than 5%. CONCLUSIONS: Our developed alignment instrument can reduce costs by eliminating the need for shortening inspection times for probe manufacturers.

Evaluation of Dermorphin Metabolism Using Zebrafish Water Tank Model and Human Liver Microsomes.

BACKGROUND: Dermorphin is a heptapeptide with an analgesic potential higher than morphine that does not present the same risk for the development of tolerance. These pharmacological features make dermorphin a potential doping agent in competitive sports and it is already prohibited for racehorses. For athletes, the development of an efficient strategy to monitor for its abuse necessitates an investigation of the metabolism of dermorphin in humans. METHODS: Here, human liver microsomes and zebrafish were utilized as model systems of human metabolism to evaluate the presence and kinetics of metabolites derived from dermorphin. Five hours after its administration, the presence of dermorphin metabolites could be detected in both models by liquid chromatography coupled to highresolution mass spectrometry. RESULTS: Although the two models showed common results, marked differences were also observed in relation to the formed metabolites. Six putative metabolites, based on their exact masses of m/z 479.1915, m/z 501.1733, m/z 495.1657, m/z 223.1073, m/z 180.1017 and m/z 457.2085, are proposed to represent the metabolic pattern of dermorphin. The major metabolite generated from the administration of dermorphin in both models was YAFG-OH (m/z 457.2085), which is the N-terminal tetrapeptide previously identified from studies on rats. CONCLUSION: Its extensive characterization and commercial availability suggest that it could serve as a primary target analyte for the detection of dermorphin misuse. The metabolomics approach also allowed the assignment of other confirmatory metabolites.

Phase II stanozolol metabolism study using the zebrafish water tank (ZWT) model.

Stanozolol (STAN) is an androgen anabolic steroid often misused in sports competitions and prohibited at all times by the World Anti-Doping Agency (WADA). It can be long term detected by the analysis of human urine for traces of intact glucuronide metabolites. The Zebrafish Water Tank (ZWT) experimental setup can produce phase I STAN metabolites. In the present study, we investigated the in vivo phase II metabolism of STAN through the ZWT model to determine whether the ZWT produces metabolites relevant for doping control. We added STAN to a 200 mL recipient containing eight fish at 32 ± 1 °C. We analyzed the noninvasive samples (recipient water) both with and without pretreatment using Liquid Chromatography coupled with High-Resolution Mass Spectrometry (LC-HRMS/MS) in positive ionization mode. Our data show that four hydroxylated-sulfate and four hydroxylated-glycoconjugate metabolites were formed, two of the last ones being 3'OH-STAN-Glucuronide and 16ß-OH-STAN-Glucuronide. Additionally, two STAN-Glucuronide derivatives were produced: one was confirmed to be 17epi-STAN-N-Glucuronide, and the other was presumed to be STAN-O-Glucuronide. After eight hours of the experiment, STAN-O-Glucuronide was the most intense phase II metabolite produced. The accumulation curves suggest that high concentrations of fish and substrate in water are required to form phase II metabolites.

A Gel Pad Designed to Measure Muscle Volume Using Freehand 3-Dimensional Ultrasonography.

We developed an innovative gel pad that covers the entire lower leg to remove artifacts due to the pressure of the transducer in freehand 3-dimensional ultrasonography. In comparison to the reference method in water, this study showed that this new method was valid (bias, 3.4 mL; limit of agreement, 7.7 mL for a volume of ≈220 mL) and reliable (coefficient of variation, <1.1%) for the measurement of gastrocnemius medialis muscle volume. Considering that it is easier to use than the water tank technique, it has much promise for volumetric measurement of many muscles.

A laboratory study of the expiratory airflow and particle dispersion in the stratified indoor environment.

Understanding the role of human expiratory flows on respiratory infection in ventilated environments is useful for taking appropriate interventions to minimize the infection risk. Some studies have predicted the lock-up phenomenon of exhaled flows in stratified environments; however, there is a lack of high-quality experimental data to validate the theoretical models. In addition, how thermal stratification affects the transport of exhaled particles has not been explored so far. In this study, a water tank experiment was conducted according to the similarity protocols to mimic how the expiratory airflow and particles behaved in both uniform and stratified environments. The lock-up phenomenon was visualized and compared with the predicted results by an integral model. Results showed that our previously developed theoretical model of a respiratory airflow was effective to predict the airflow dispersion in stratified environments. Stratification frequency (N) of the background fluid and the Froude Number F r 0 of the thermal flow jointly determined the lock-up layer in a power law. For the particle dispersion, it indicated that small particles such as fine droplets and droplet nuclei would be 'locked' by indoor thermal stratification, and disperse with the thermal flow over a long distance, potentially increasing the long-range airborne infection risk. Large particles such as large droplets can deposit within a short distance, hardly affected by thermal stratification, however, droplet infection could happen to the susceptible people at a close contact with the infector. This study could give some guidance in view of cross-infection control indoors for stratified environment.

The effect of the horizontal metallic drive on reference dosimetry in the SNC 3D scanner water tank.

Accurate quantification of absorbed radiation dose is important for safe and effective delivery of radiation therapy. An important aspect to this is reference dosimetry, which is performed under reference conditions specified by international codes of practice. Such measurements are usually performed in a water phantom. In the Sun Nuclear Corporation (SNC) three-dimensional (3D) scanner water tank system the detector holder is fixed to a horizontal metallic drive rail (MDR) which is in close proximity to the active volume of the detector. In this project, the dosimetric effects of the MDR on reference dosimetry were investigated for MV photons, MeV electrons, and kV photons by comparing reference dosimetry measurements in the SNC 3D scanner against similar measurements in a Standard Imaging (SI) one-dimensional (1D) tank and against measurements in the SNC 3D scanner with an additional, custom-made spacer placed beneath the chamber holder to increase the chamber - MDR separation. A second experiment investigated the difference in chamber reading dependent on chamber to MDR separation by fixing the chamber in the tank independently of the MDR and successively moving the MDR vertically to alter the separation. The results showed that measurements in the SNC 3D scanner agree with both SI 1D tank and SNC 3D scanner with spacer to within ±0.3% for MV photons, ±0.1% for electrons and ±1.2% for kV photons within the calculated setup uncertainty. The second experiment showed that the contribution of backscatter from the MDR was significant if the distance between MDR and chamber was reduced below the chamber's designed position in the SNC 3D scanner. The exception was for kV photons where the contribution of backscatter from the MDR was measured to be 0.5% at the designed distance. Further investigation would be useful for kV photons, where the experiment showed relatively large measurement uncertainties.

Comprehensive Zebrafish Water Tank Experiment for Metabolic Studies of Testolactone.

Testolactone is a potent steroid aromatase (CYP19A1) inhibitor, and its main effect is a reduction in estradiol and estrone and an increase in testosterone and androstenedione levels. In this work, we evaluated a zebrafish water tank (ZWT) as a model to investigate testolactone biotransformation and the possibility to increase knowledge regarding the applicability of the ZWT on steroid hormone elimination research, as well as on the impact of steroid hormones on the endogenous metabolism of zebrafish. High-resolution mass spectrometry combined with SIEVE software was used to discriminate the peaks of interest based on significant changes in the relative signal intensity of the m/z values between different ZWT experiments. The metabolites, 4,5-dihydrotestolactone and 1,2,4,5-tetrahydrotestolactone, the same metabolites as those described in humans, were detected in ZWT, both in quite similar proportions. The presence of testolactone in the ZWT caused a rise in testosterone and androstenedione in the water tank, similar to that in human serum. These data suggest that, while the concentration of testolactone was high enough to inhibit the aromatase enzyme, an accumulation of androgens in the water occurred, indicating that the ZWT can be considered a model to investigate the impact of steroids on live organisms.

Packaging Technology for an Implantable Inner Ear MEMS Microphone.

Current cochlear implant (CI) systems provide substantial benefits for patients with severe hearing loss. However, they do not allow for 24/7 hearing, mainly due to the external parts that cannot be worn in all everyday situations. One of the key missing parts for a totally implantable CI (TICI) is the microphone, which thus far has not been implantable. The goal of the current project was to develop a concept for a packaging technology for state-of-the-art microelectromechanical systems (MEMS) microphones that record the liquid-borne sound inside the inner ear (cochlea) as a microphone signal input for a TICI. The packaging concept incorporates requirements, such as biocompatibility, long-term hermeticity, a high sensing performance and a form factor that allows sensing inside the human cochlea and full integration into the existing CI electrode array. The present paper (1) describes the sensor packaging concept and the corresponding numerical and experimental design verification process and (2) gives insight into new engineering solutions for sensor packaging. Overall, a packaging concept was developed that enables MEMS microphone technology to be used for a TICI system.