ABSTRACT
One goal of microbial ecology researchers is to capture the maximum amount of information from all organisms in a sample. The recent COVID-19 pandemic, caused by the RNA virus SARS-CoV-2, has highlighted a gap in traditional DNA-based protocols, including the high-throughput methods the authors previously established as field standards. To enable simultaneous SARS-CoV-2 and microbial community profiling, the authors compared the relative performance of two total nucleic acid extraction protocols with the authors' previously benchmarked protocol. The authors included a diverse panel of environmental and host-associated sample types, including body sites commonly swabbed for COVID-19 testing. Here the authors present results comparing the cost, processing time, DNA and RNA yield, microbial community composition, limit of detection and well-to-well contamination between these protocols.
Subject(s)
DNA, Viral/isolation & purification , High-Throughput Nucleotide Sequencing/methods , Microbiota/genetics , RNA, Ribosomal, 16S/isolation & purification , SARS-CoV-2/genetics , Animals , Biodiversity , Cats , Chemical Fractionation/methods , Feces/microbiology , Feces/virology , Female , Fermented Foods/microbiology , Humans , Limit of Detection , Male , Metagenomics/methods , Mice , Saliva/microbiology , Saliva/virology , Skin/microbiology , Skin/virologyABSTRACT
Human consumption water can be obtained from several different sources. One of these sources, the underground source, which due to its low cost, easy perforation and water capitation from the free water-bearing (flat well) is more frequently used in Brazil. The waters good aspect gives the consumers a purity sensation, what leads them not to treat water, not even for a dis infection process, what would certainly minimize diseases spreading. Microbiologically contaminated water can cause a large variety of diseases. The goal of this study is to evaluate water quality relating PID (Parasite Infectious Diseases) medication consume. There were evaluated 385 water findings from rural communities from the city of Bandeirantes PR, coming from artesian or flat wells (brute water), it was evaluated also the PID medication consume from Municipal Health Office from Bandeirantes PR SUS Drugstore of Bandeirantes PR. The results have shown that the waters presented high Coliforms sample percentage...
